FastQCFastQC Report
Thu 2 Feb 2017
SRR4050248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498597
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA350477.0291237211615805No Hit
CTTCCGTACTCTGCGTTGATACCAC227404.560797598060157No Hit
GAGTACGGAAGCAGTGGTATCAACG198123.9735497806846007No Hit
TCCGTACTCTGCGTTGATACCACTG193343.877680772246925No Hit
GCTTCCGTACTCTGCGTTGATACCA166203.333353389611249No Hit
CCCATGTACTCTGCGTTGATACCAC105942.1247620824032234No Hit
GTACATGGGAAGCAGTGGTATCAAC96681.939040948902621No Hit
GCGTTGATACCACTGCTTCCGTACT85781.720427519620054No Hit
GTATCAACGCAGAGTACGGAAGCAG80321.610920242199612No Hit
GTACATGGAAGCAGTGGTATCAACG78381.5720110630428983No Hit
TCCATGTACTCTGCGTTGATACCAC74271.4895797608088295No Hit
TATCAACGCAGAGTACGGAAGCAGT68671.3772646044801713No Hit
ACTCTGCGTTGATACCACTGCTTCC58021.1636652446765623No Hit
CAGTGGTATCAACGCAGAGTACGGA47290.948461382639687No Hit
ACGCAGAGTACGGAAGCAGTGGTAT45100.9045381340040152No Hit
CATGTACTCTGCGTTGATACCACTG43680.8760582193635341No Hit
GGTATCAACGCAGAGTACGGAAGCA42760.8576064436809688No Hit
ATACCACTGCTTCCGTACTCTGCGT38660.7753757042260583No Hit
GAGTACATGGGAAGCAGTGGTATCA35250.7069837965330718No Hit
AAGCAGTGGTATCAACGCAGAGTAC35040.7027719781707471No Hit
GAAGCAGTGGTATCAACGCAGAGTA31340.6285637498821693No Hit
GTGGTATCAACGCAGAGTACGGAAG31030.6223463037282615No Hit
GTACGGGAAGCAGTGGTATCAACGC29570.593064137971147No Hit
GAGTACATGGAAGCAGTGGTATCAA27870.558968465514233No Hit
GTATCAACGCAGAGTACATGGGAAG23720.47573491216353087No Hit
ATCAACGCAGAGTACGGAAGCAGTG23290.46711071265972315No Hit
CGGAAGCAGTGGTATCAACGCAGAG21880.4388313607984003No Hit
GCGTTGATACCACTGCTTCCCATGT21530.43181166352785916No Hit
CCCGTACTCTGCGTTGATACCACTG20560.41235707394950233No Hit
GCTTCCCATGTACTCTGCGTTGATA20470.41055200893707744No Hit
CATGGAAGCAGTGGTATCAACGCAG20280.4067413161330694No Hit
GTTGATACCACTGCTTCCGTACTCT20230.4057385022372778No Hit
CCACTGCTTCCGTACTCTGCGTTGA20090.4029306233290613No Hit
GTACTCTGCGTTGATACCACTGCTT20040.4019278094332697No Hit
GATACCACTGCTTCCGTACTCTGCG19980.40072443275831987No Hit
TATCAACGCAGAGTACATGGGAAGC19790.39691373995431184No Hit
TTCCCGTACTCTGCGTTGATACCAC19730.3957103632793619No Hit
GCTTCCATGTACTCTGCGTTGATAC17780.3566006213434898No Hit
CTGCTTCCGTACTCTGCGTTGATAC16940.3397533478941911No Hit
ACGCAGAGTACATGGGAAGCAGTGG16830.3375471573234496No Hit
CAGTGGTATCAACGCAGAGTACATG16800.33694546898597466No Hit
GAGTACGGGAAGCAGTGGTATCAAC15600.31287793548697646No Hit
CTTCCCGTACTCTGCGTTGATACCA14730.29542897370020277No Hit
AAAAAGTACTCTGCGTTGATACCAC14430.28941209032545323No Hit
GCAGTGGTATCAACGCAGAGTACGG13990.2805873280424872No Hit
GCAGAGTACGGAAGCAGTGGTATCA13730.27537269578437096No Hit
CCATGTACTCTGCGTTGATACCACT13730.27537269578437096No Hit
GCGTTGATACCACTGCTTCCATGTA13700.27477100744689603No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12540.25150572506453106No Hit
GTATCAACGCAGAGTACATGGAAGC12120.2430820883398817No Hit
CACTGCTTCCGTACTCTGCGTTGAT11830.2372657677442905No Hit
TGATACCACTGCTTCCGTACTCTGC11570.2320511354861742No Hit
GTATCAACGCAGAGTACTTTTTTTT11190.2244297498781581No Hit
GCCCATGTACTCTGCGTTGATACCA11090.22242412208657494No Hit
TATCAACGCAGAGTACATGGAAGCA11030.221220745411625No Hit
GGTATCAACGCAGAGTACATGGGAA10740.2154044248160338No Hit
CCCCATGTACTCTGCGTTGATACCA10600.21259654590781735No Hit
GGGAAGCAGTGGTATCAACGCAGAG10410.2087858531038093No Hit
GTATCAACGCAGAGTACGGGAAGCA10250.20557684863727618No Hit
ACGGAAGCAGTGGTATCAACGCAGA10160.20377178362485132No Hit
AAAGTACTCTGCGTTGATACCACTG10060.20176615583326812No Hit
TACGGAAGCAGTGGTATCAACGCAG9670.19394420744609375No Hit
TACCACTGCTTCCGTACTCTGCGTT9480.1901335146420857No Hit
ACGCAGAGTACATGGAAGCAGTGGT9230.18511944516312773No Hit
GCTTCCCGTACTCTGCGTTGATACC9030.18110818957996136No Hit
TCAACGCAGAGTACGGAAGCAGTGG9010.18070706402164474No Hit
TATCAACGCAGAGTACTTTTTTTTT8940.1793031245675365No Hit
CTGCGTTGATACCACTGCTTCCGTA8830.17709693399679502No Hit
GCGTTGATACCACTGCTTCCCGTAC8750.17549243176352847No Hit
CATGGGAAGCAGTGGTATCAACGCA8630.17308567841362865No Hit
TATCAACGCAGAGTACGGGAAGCAG8610.172684552855312No Hit
TCCCATGTACTCTGCGTTGATACCA8410.16867329727214564No Hit
ATACCACTGCTTCCCATGTACTCTG8280.16606598114308752No Hit
CTGCTTCCCATGTACTCTGCGTTGA8220.1648626044681376No Hit
GCAGTGGTATCAACGCAGAGTACAT8110.16265641389739607No Hit
GTACAAGCAGTGGTATCAACGCAGA7930.15904628387254635No Hit
TACCACTGCTTCCCATGTACTCTGC6990.14019338263166445No Hit
ATACCACTGCTTCCATGTACTCTGC6770.13578100149018144No Hit
GTGGTATCAACGCAGAGTACATGGG6770.13578100149018144No Hit
CTCTGCGTTGATACCACTGCTTCCG6760.13558043871102315No Hit
ACATGGGAAGCAGTGGTATCAACGC6520.1307669320112235No Hit
GCAGAGTACATGGGAAGCAGTGGTA6250.1253517369739489No Hit
CTTCCCATGTACTCTGCGTTGATAC5880.11793091414509112No Hit
CCACTGCTTCCCATGTACTCTGCGT5850.11732922580761616No Hit
ACATGGAAGCAGTGGTATCAACGCA5720.11472190967855803No Hit
GGTATCAACGCAGAGTACATGGAAG5680.11391965856192476No Hit
AGCAGTGGTATCAACGCAGAGTACG5610.11251571910781653No Hit
GGAAGCAGTGGTATCAACGCAGAGT5560.11151290521202493No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5510.11051009131623335No Hit
GCTTGTACTCTGCGTTGATACCACT5360.10750164962885858No Hit
ATCAACGCAGAGTACATGGGAAGCA5150.10328983126653389No Hit
GATACCACTGCTTCCCATGTACTCT5090.10208645459158398No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5050.10128420347495072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGAGC250.006040813418.9921728
GTCCTGT402.695962E-416.6733671
CTTCGTA402.7188315E-416.654922
CGTCCGT350.002125749416.3330961
GTCCGTA350.00214153116.3150252
GGACGGA1003.8198777E-1115.2442211
AAAAAGT2200.014.7245311
TAGAACA653.298861E-614.6416874
AGAACAG653.3613887E-614.6152315
GACGGAA1058.54925E-1114.5022442
GTAGAAC400.005209900414.2756463
GGACATG500.001464625213.3386941
GTTATCA500.001464625213.3386941
TGTAGAA500.001476898713.3239372
AAAAGTA2550.013.0626832
TTATCAA550.00302242212.112672
GCCCATG2000.011.9095481
GTACAAA657.81379E-411.7263231
ATAGAAC657.886723E-411.713353
ACCCCGT600.005740630411.1155781
Produced by FastQC (version 0.11.5)