FastQCFastQC Report
Thu 2 Feb 2017
SRR4050247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8460008
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA168560.19924331040821708No Hit
GTATCAACGCAGAGTACTTTTTTTT156740.18527169241447525No Hit
GTATCTGATCGTCTTCGAACCTCCG135210.15982254390303177No Hit
GATTAAGAGGGACGGCCGGGGGCAT126390.14939702184678785No Hit
GAATAGGACCGCGGTTCTATTTTGT125770.14866416201970495No Hit
CTATTGGAGCTGGAATTACCGCGGC123530.14601641038637314No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG117860.13931428906450205No Hit
GTCCTATTCCATTATTCCTAGCTGC116260.13742303789783653No Hit
ATACAGGACTCTTTCGAGGCCCTGT102800.12151288745826244No Hit
ATATTAAAGTTGCTGCAGTTAAAAA102410.12105189498638773No Hit
ATCAGATACCGTCGTAGTTCCGACC100110.11833322143430597No Hit
GAACTACGACGGTATCTGATCGTCT99350.11743487713013984No Hit
CTTTAATATACGCTATTGGAGCTGG99200.11725757233326493No Hit
CTCTTAATCATGGCCTCAGTTCCGA98160.11602825907493233No Hit
GCTTTGAACACTCTAATTTTTTCAA92680.10955072382910276No Hit
GTATCAACGCAGAGTACGGGCGGTG92180.10895970783951978No Hit
GTCTTGCGCCGGTCCAAGAATTTCA91260.10787223841868708No Hit
GTCCTGTATTGTTATTTTTCGTCAC89390.10566183861764669No Hit
TCGTAGTTCCGACCATAAACGATGC89310.10556727605931342No Hit
CTTATGACCCGCACTTACTGGGAAT86060.10172567212702399No Hit
TATCAACGCAGAGTACTTTTTTTTT84700.10011810863535825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51450.016.026281
GTATCAA183250.013.689951
ACGCTTA4250.012.30464517
TAGATCG4200.012.0001465
CGAACGA10350.011.75927216
CTCGACG4550.011.494102513
CGGTTCT23500.011.40996512
CGCTTAC4600.011.36855718
CGCGCCA5700.011.34519810
TCGACGC4500.011.19886914
CGACGCT4750.011.00981215
CGTCGTA16500.011.00848210
AACCGCG12650.010.9000797
AGGCCCG22600.010.81440610
CGAGCCG21550.010.6777315
TAGGCCG909.429088E-510.5661655
GCTCGTA10100.010.5456829
ACTCTAA19500.010.43655510
GCGCGCT8750.010.43215415
CGGTCCA19250.010.37448510
Produced by FastQC (version 0.11.5)