##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050247_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8460008
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.670057404201035	31.0	31.0	34.0	27.0	34.0
2	30.793620762533557	31.0	31.0	34.0	27.0	34.0
3	30.898012034976798	33.0	31.0	34.0	27.0	34.0
4	34.423982341387855	37.0	35.0	37.0	31.0	37.0
5	34.43280053635883	37.0	35.0	37.0	31.0	37.0
6	34.46494755087702	37.0	35.0	37.0	31.0	37.0
7	34.38396772201634	37.0	35.0	37.0	31.0	37.0
8	34.353826142954	37.0	35.0	37.0	31.0	37.0
9	35.92042891685209	39.0	37.0	39.0	31.0	39.0
10	35.81565525706359	39.0	37.0	39.0	30.0	39.0
11	35.70275465460553	39.0	37.0	39.0	30.0	39.0
12	35.62801394514048	39.0	37.0	39.0	30.0	39.0
13	35.63135495852959	39.0	37.0	39.0	30.0	39.0
14	36.814841900858724	40.0	38.0	41.0	31.0	41.0
15	36.76575755011106	40.0	37.0	41.0	30.0	41.0
16	36.77212267411567	40.0	37.0	41.0	31.0	41.0
17	36.74565248638063	40.0	37.0	41.0	30.0	41.0
18	36.7125375058747	40.0	37.0	41.0	30.0	41.0
19	36.73215214453698	40.0	38.0	41.0	30.0	41.0
20	36.69437936701715	40.0	37.0	41.0	30.0	41.0
21	36.64762799278677	40.0	37.0	41.0	30.0	41.0
22	36.571618253788884	40.0	37.0	41.0	30.0	41.0
23	36.49835071077947	40.0	37.0	41.0	30.0	41.0
24	36.43892440763649	40.0	37.0	41.0	30.0	41.0
25	36.21567887406253	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.574264377367367
1101	2	0.5948975546942883
1101	3	0.5929471178326402
1101	4	0.600132088434961
1101	5	0.5953406251542788
1101	6	0.6355566574111151
1101	7	0.5989273050781136
1101	8	0.624950226486817
1101	9	0.6300980575996107
1101	10	0.6487664950927083
1101	11	0.5311245895596812
1101	12	0.625984192324637
1101	13	0.5630877888269765
1101	14	0.7538056204493344
1101	15	0.7525448382466067
1101	16	0.7809148491914399
1101	17	0.7779304981155519
1101	18	0.7890751645398453
1101	19	0.8223739920140787
1101	20	0.7689435267449412
1101	21	0.7664984007095654
1101	22	0.8225229003788854
1101	23	0.8375461727889046
1101	24	0.8226547433670603
1101	25	0.8781246246346868
1102	1	0.4683830582717867
1102	2	0.4314611856604884
1102	3	0.47108353464665953
1102	4	0.4563706675885726
1102	5	0.42215313837216684
1102	6	0.3716167704111868
1102	7	0.39714478483977445
1102	8	0.41520303700712446
1102	9	0.5109610150735051
1102	10	0.5673864332279166
1102	11	0.45554426895507305
1102	12	0.421894979084513
1102	13	0.4065091221894548
1102	14	0.6237125865355395
1102	15	0.5465687936126002
1102	16	0.592344577777304
1102	17	0.5303906164416716
1102	18	0.6028151991302337
1102	19	0.6517859700290813
1102	20	0.5364204701901016
1102	21	0.5357458340704326
1102	22	0.5842673755588876
1102	23	0.6349490250564784
1102	24	0.5505212357456344
1102	25	0.6466777546525435
1103	1	0.5477478973471648
1103	2	0.5170334578231426
1103	3	0.5227765301757827
1103	4	0.5400848012442836
1103	5	0.5404691001190045
1103	6	0.4919499518642212
1103	7	0.5124148281588319
1103	8	0.5332511949073222
1103	9	0.5406684487983568
1103	10	0.5941288836166763
1103	11	0.5521792818794253
1103	12	0.47218291987382344
1103	13	0.48735276383652604
1103	14	0.6725701466999112
1103	15	0.6938013450204821
1103	16	0.6522195185020152
1103	17	0.5416117155852191
1103	18	0.6464963570378828
1103	19	0.6441520431651497
1103	20	0.6506271331904472
1103	21	0.5994686591280498
1103	22	0.699754843715624
1103	23	0.6894458770128153
1103	24	0.7451509697708616
1103	25	0.7392100969295754
1104	1	0.45639665026174825
1104	2	0.44132431308154096
1104	3	0.443189310604442
1104	4	0.43077698169923906
1104	5	0.4735593627177437
1104	6	0.43150564418150594
1104	7	0.44162536025996957
1104	8	0.45536353842059185
1104	9	0.5497639669942558
1104	10	0.5402517023802602
1104	11	0.46923988901001934
1104	12	0.45584744652969533
1104	13	0.44874989301661117
1104	14	0.500569622520807
1104	15	0.3910472690516187
1104	16	0.48985884003148783
1104	17	0.5577424869621481
1104	18	0.5653008898051795
1104	19	0.5446288868576303
1104	20	0.5994836064615541
1104	21	0.5416768782278112
1104	22	0.5189708546753948
1104	23	0.5123415697307934
1104	24	0.5499686588087584
1104	25	0.6461758901706958
1105	1	0.3460099358796249
1105	2	0.4203601389361076
1105	3	0.4300194731632736
1105	4	0.4518972959091059
1105	5	0.45462868888681385
1105	6	0.3915052766245637
1105	7	0.4395436685675591
1105	8	0.4625196389355324
1105	9	0.4680437570041036
1105	10	0.4345232447353453
1105	11	0.47392297081920276
1105	12	0.38972387455012125
1105	13	0.47000544916500786
1105	14	0.5739227543049026
1105	15	0.5537045726284973
1105	16	0.4381945960560998
1105	17	0.49125290797967125
1105	18	0.5712287939799339
1105	19	0.5372456681933997
1105	20	0.48406444882977695
1105	21	0.5067943177829122
1105	22	0.5621781630619722
1105	23	0.5608772229397516
1105	24	0.5042281055187274
1105	25	0.5433547662564564
1106	1	0.3076126208052514
1106	2	0.2796383067948831
1106	3	0.3290523554536229
1106	4	0.38782057542604775
1106	5	0.4081323407936921
1106	6	0.3376163078900234
1106	7	0.3309950999938067
1106	8	0.3304938106237856
1106	9	0.30308551827479135
1106	10	0.38045164383223096
1106	11	0.386988445296538
1106	12	0.41221617931841337
1106	13	0.3638181797934408
1106	14	0.41598031110606826
1106	15	0.46283757306893136
1106	16	0.39135548664327047
1106	17	0.42759375269780264
1106	18	0.4813418330323316
1106	19	0.41515087613579027
1106	20	0.4212260702261972
1106	21	0.4175254023053441
1106	22	0.38886530447751966
1106	23	0.23717895602344896
1106	24	0.42968922273086463
1106	25	0.46162305442335594
1107	1	0.2353002027874851
1107	2	0.1935748114401541
1107	3	0.14820625163021361
1107	4	0.13081074912357593
1107	5	0.23511637191332113
1107	6	0.12949085326007292
1107	7	0.20158852443151432
1107	8	0.23981445129810908
1107	9	0.30119140263238364
1107	10	0.3132094602730433
1107	11	0.2369660953049575
1107	12	0.19219986170534042
1107	13	0.24326350723197265
1107	14	0.2900659225055193
1107	15	0.2576605796927396
1107	16	0.20543438788703128
1107	17	0.2284569497311324
1107	18	0.24352736999313152
1107	19	0.25072278416208604
1107	20	0.2959271811158004
1107	21	0.24568971408886853
1107	22	0.22380709599611492
1107	23	0.17368895838267662
1107	24	0.18724320355688917
1107	25	0.28379451330697947
1108	1	0.25851897501143384
1108	2	0.2091100033541835
1108	3	0.21479470328286965
1108	4	0.23377341246818872
1108	5	0.23979265507369973
1108	6	0.23530860016465738
1108	7	0.24384684345022833
1108	8	0.21740816365264948
1108	9	0.22680977397630642
1108	10	0.1499210290597759
1108	11	0.1261743166472229
1108	12	0.14449857356601825
1108	13	0.17877031357814133
1108	14	0.09584609114988751
1108	15	0.24590232231459197
1108	16	0.18811873453747552
1108	17	0.17997774566735814
1108	18	0.13764947338810174
1108	19	0.27041965051965633
1108	20	0.2640925657989186
1108	21	0.29547437573707214
1108	22	0.33832013814203066
1108	23	0.2693241215836366
1108	24	0.2961451786233624
1108	25	0.2634111464488029
1109	1	0.08296746807783961
1109	2	0.06484096874214629
1109	3	0.12787651964598012
1109	4	0.11479926983602695
1109	5	0.14541017751071195
1109	6	0.07997847637169997
1109	7	0.11720986888988705
1109	8	0.1474460325072826
1109	9	0.1324194778997665
1109	10	-0.004020806934157406
1109	11	0.0400996597925527
1109	12	0.11310996655555527
1109	13	0.04131813822931463
1109	14	0.12388523389386563
1109	15	0.14448899449406838
1109	16	0.06581430882127393
1109	17	0.03741916079432883
1109	18	0.1295203920921182
1109	19	0.1337644886245073
1109	20	0.07982971499588842
1109	21	0.13018086544349217
1109	22	0.1591379141719642
1109	23	0.19240686162368092
1109	24	0.07444862660386775
1109	25	0.12881817241679272
1110	1	0.05206591415067052
1110	2	0.008461015498703262
1110	3	0.06868000791305917
1110	4	0.034718483512143905
1110	5	0.049934500016561856
1110	6	0.11377490803852908
1110	7	0.04937025197234135
1110	8	0.04506572575854051
1110	9	0.08830654438310148
1110	10	-0.07601263229454958
1110	11	0.042811725221369556
1110	12	-0.04318060869951523
1110	13	-0.060032765434918645
1110	14	-0.09026571212908152
1110	15	0.011447741091231478
1110	16	0.021972743014856633
1110	17	0.06897986034478265
1110	18	0.0016984483599031819
1110	19	-0.011836138631927895
1110	20	0.01781115699596114
1110	21	0.027885058915401828
1110	22	-0.027647154779991467
1110	23	-4.1966116786085195E-4
1110	24	-0.005860353062665524
1110	25	0.06452535232567413
1111	1	-0.0477252554352674
1111	2	-0.11909978468863258
1111	3	-0.1195511019644222
1111	4	-0.03390132804999979
1111	5	-0.08707620980342057
1111	6	-0.08593450598861807
1111	7	-0.14979452808554328
1111	8	-0.10759883414475269
1111	9	-0.15574152888000725
1111	10	-0.11349705873081462
1111	11	-0.19725110924859734
1111	12	-0.24386991406884562
1111	13	-0.23782263714853968
1111	14	-0.22193255359336916
1111	15	-0.20241505875073074
1111	16	-0.20624161567528176
1111	17	-0.21359811370862047
1111	18	-0.16648914595170083
1111	19	-0.14146604159212472
1111	20	-0.11367389871173827
1111	21	-0.10734957524373101
1111	22	-0.12944682811941277
1111	23	-0.1465171611701237
1111	24	-0.16981702374666696
1111	25	-0.11573095146414403
1112	1	-0.12415351593554291
1112	2	-0.1527540187736527
1112	3	-0.13480764561471048
1112	4	-0.16414730772186203
1112	5	-0.1079202486760309
1112	6	-0.16155206519644594
1112	7	-0.13768390889838855
1112	8	-0.183891992508876
1112	9	-0.24125507625334563
1112	10	-0.2816032222902223
1112	11	-0.2598635616487641
1112	12	-0.3245891493571307
1112	13	-0.23233034159779464
1112	14	-0.29690247191275887
1112	15	-0.28347731367350804
1112	16	-0.21015400366525938
1112	17	-0.2033246004514453
1112	18	-0.22493696662241547
1112	19	-0.33925548044491904
1112	20	-0.22401810391834687
1112	21	-0.2841658894238819
1112	22	-0.24680714295073614
1112	23	-0.2728317306128005
1112	24	-0.2522803771816413
1112	25	-0.2081659396438127
1113	1	-0.26494955319543223
1113	2	-0.24291527324504614
1113	3	-0.15020000823351864
1113	4	-0.055139173059686186
1113	5	-0.10357011703269592
1113	6	-0.18690908743796797
1113	7	-0.17583872249555554
1113	8	-0.2039302965843106
1113	9	-0.1335443779922727
1113	10	-0.2552537057865578
1113	11	-0.21630258355945386
1113	12	-0.3779532052125063
1113	13	-0.31258586579328806
1113	14	-0.25529352843668107
1113	15	-0.16228002270037933
1113	16	-0.19376518369958262
1113	17	-0.2504427916995766
1113	18	-0.1797535791843572
1113	19	-0.26723964016756696
1113	20	-0.22273122138417278
1113	21	-0.2500919330936284
1113	22	-0.2596267743493996
1113	23	-0.2896483914209895
1113	24	-0.31551367708721756
1113	25	-0.305053108898548
1114	1	-0.34708606445141754
1114	2	-0.32884845288430853
1114	3	-0.4225815333982226
1114	4	-0.3343816465244771
1114	5	-0.3002338518822114
1114	6	-0.3703429016659143
1114	7	-0.46083083061484587
1114	8	-0.4320011313060732
1114	9	-0.4615390881863277
1114	10	-0.44292223739534364
1114	11	-0.5148383550364954
1114	12	-0.5879939912459093
1114	13	-0.44468539854798905
1114	14	-0.6867348908750941
1114	15	-0.7159390140647517
1114	16	-0.63132022055796
1114	17	-0.5874153127036763
1114	18	-0.6476583553651878
1114	19	-0.49676485814747906
1114	20	-0.6127758828480836
1114	21	-0.6146325013299361
1114	22	-0.7343447088180639
1114	23	-0.595026752521278
1114	24	-0.6090642190729625
1114	25	-0.6882721553337277
1115	1	-0.4603020059078311
1115	2	-0.48326045341739743
1115	3	-0.49178164088657894
1115	4	-0.3212011489380231
1115	5	-0.38687859179440665
1115	6	-0.40284142063614325
1115	7	-0.4610027407991879
1115	8	-0.4923820822181426
1115	9	-0.4137017673434755
1115	10	-0.512105057964142
1115	11	-0.5326920650852713
1115	12	-0.5542044602083536
1115	13	-0.5204748823270506
1115	14	-0.6277704638655379
1115	15	-0.5501559180916473
1115	16	-0.6140658678332187
1115	17	-0.5166445869872263
1115	18	-0.534092928452921
1115	19	-0.5740162557333832
1115	20	-0.569247602297942
1115	21	-0.6450049763620243
1115	22	-0.8310499959559223
1115	23	-0.7037390584351968
1115	24	-0.6775740262978545
1115	25	-0.6781067792787141
1116	1	-0.40244825156552366
1116	2	-0.5235179612427139
1116	3	-0.4710422090761561
1116	4	-0.5188779738160392
1116	5	-0.49492851891189815
1116	6	-0.4895164836027419
1116	7	-0.42633787143367385
1116	8	-0.5738511226429495
1116	9	-0.5309778724191077
1116	10	-0.5160925796332876
1116	11	-0.6430842585615295
1116	12	-0.7322213815906764
1116	13	-0.6851706628754073
1116	14	-0.7228021829067544
1116	15	-0.7591043742398114
1116	16	-0.7594062605711116
1116	17	-0.7498340519939006
1116	18	-0.7307818369475001
1116	19	-0.7884093042166427
1116	20	-0.7303090518841842
1116	21	-0.8312017888660677
1116	22	-0.8519030169505157
1116	23	-0.8150811497674013
1116	24	-0.8505907763847986
1116	25	-0.8857516097991862
1201	1	0.48133745385417015
1201	2	0.5357696088239159
1201	3	0.5190027537026651
1201	4	0.524639508455806
1201	5	0.5747942673221687
1201	6	0.6038989909897126
1201	7	0.582932882484279
1201	8	0.5684205474154496
1201	9	0.6473594120951844
1201	10	0.6131905673367442
1201	11	0.49903939288800103
1201	12	0.5716204183642759
1201	13	0.5667231788407321
1201	14	0.6676346827686146
1201	15	0.6963993381894724
1201	16	0.6418415400192643
1201	17	0.6464217508842012
1201	18	0.6854897566851221
1201	19	0.682146128656619
1201	20	0.6349114034651677
1201	21	0.6931309878794423
1201	22	0.6897605094324746
1201	23	0.7102251937768642
1201	24	0.6986111150269281
1201	25	0.649441433887219
1202	1	0.3876544722015183
1202	2	0.3892096315495195
1202	3	0.437092770543849
1202	4	0.5525713427797996
1202	5	0.5602373097721411
1202	6	0.475574137707909
1202	7	0.47462503269547796
1202	8	0.4672730125174951
1202	9	0.4351670339261915
1202	10	0.5953461535295119
1202	11	0.42789376661097833
1202	12	0.4250502096389255
1202	13	0.41584838174888716
1202	14	0.5125847974813951
1202	15	0.5210059268474865
1202	16	0.503951672857248
1202	17	0.49086021269750546
1202	18	0.5885598160366499
1202	19	0.5149567071626748
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2210	15	0.024403989408455118
2210	16	0.03955922614915863
2210	17	-0.05828305585356475
2210	18	0.02109249217409115
2210	19	0.018204272117095854
2210	20	-0.04571934432583902
2210	21	-0.043996982941571616
2210	22	-0.007202724708662345
2210	23	-0.042216414464562035
2210	24	0.04851582357234463
2210	25	-0.048070238510163676
2211	1	-0.15527856773059057
2211	2	-0.15319145179279303
2211	3	-0.15693949103024707
2211	4	-0.15035078013676184
2211	5	-0.22529549619931544
2211	6	-0.17844190995325704
2211	7	-0.1304308223576811
2211	8	-0.1549835956759935
2211	9	-0.12105950781934638
2211	10	-0.18141988654457464
2211	11	-0.030493835098745592
2211	12	-0.10398203345344115
2211	13	-0.028183007176920682
2211	14	-0.03667542105964117
2211	15	-0.004418827680858328
2211	16	-0.047813399169370996
2211	17	-0.16573602090461748
2211	18	-0.06767787216096366
2211	19	-0.15411405470904782
2211	20	-0.15860680421142348
2211	21	-0.1976693188700125
2211	22	-0.17848406455789956
2211	23	-0.12385943014282219
2211	24	-0.22587195139229266
2211	25	-0.1995950566730187
2212	1	-0.28288702732414706
2212	2	-0.24822875132852928
2212	3	-0.33800774624189955
2212	4	-0.29302113746079783
2212	5	-0.2565665702469886
2212	6	-0.3228875952864598
2212	7	-0.34252052727642024
2212	8	-0.32945159367749
2212	9	-0.2963513715032349
2212	10	-0.30410859430551795
2212	11	-0.17498260452450154
2212	12	-0.19510932929937752
2212	13	-0.19869622413478538
2212	14	-0.17872085208757937
2212	15	-0.26318369366826033
2212	16	-0.21143903129203068
2212	17	-0.2088119105422095
2212	18	-0.3427464737009842
2212	19	-0.32939557068511505
2212	20	-0.3053758697878237
2212	21	-0.24344185879278513
2212	22	-0.2568553082552967
2212	23	-0.24473755243195683
2212	24	-0.3987361299381931
2212	25	-0.3685818926577866
2213	1	-0.37102067379658976
2213	2	-0.3836474212350254
2213	3	-0.4540732476701379
2213	4	-0.409282399766866
2213	5	-0.42086162115949577
2213	6	-0.28118186697231806
2213	7	-0.39633517057176704
2213	8	-0.4178905889507476
2213	9	-0.330364071664512
2213	10	-0.28626323233359585
2213	11	-0.22633728154327315
2213	12	-0.3216018316650988
2213	13	-0.22172642342479065
2213	14	-0.4197855828037049
2213	15	-0.38096355245031077
2213	16	-0.2630143521798516
2213	17	-0.3996451486987098
2213	18	-0.3436132679290722
2213	19	-0.29363166579069855
2213	20	-0.36751523156772237
2213	21	-0.2831504885077507
2213	22	-0.31278860246092677
2213	23	-0.3195902876966059
2213	24	-0.33045639461688125
2213	25	-0.2287986433296254
2214	1	-0.40011499717587284
2214	2	-0.4527393100378774
2214	3	-0.4873129481775109
2214	4	-0.5954341431137067
2214	5	-0.5456805530841393
2214	6	-0.5006302027350245
2214	7	-0.5419473856759609
2214	8	-0.5233470479132407
2214	9	-0.5270518134019184
2214	10	-0.5595853679395049
2214	11	-0.4438405808483452
2214	12	-0.4089022524382173
2214	13	-0.4950042230670988
2214	14	-0.5722917210291882
2214	15	-0.42313684321733547
2214	16	-0.49133633597941895
2214	17	-0.5841449695104828
2214	18	-0.6359402803491037
2214	19	-0.6237476742089996
2214	20	-0.6600454800449072
2214	21	-0.6579903470072139
2214	22	-0.6368322863666407
2214	23	-0.5988489733601554
2214	24	-0.6648756161936973
2214	25	-0.6036046646216704
2215	1	-0.6019636004453659
2215	2	-0.5426739631349449
2215	3	-0.7202947239744617
2215	4	-0.6554514772891622
2215	5	-0.7081903364374753
2215	6	-0.6067757333222943
2215	7	-0.6184846104183066
2215	8	-0.7229770252931118
2215	9	-0.793881670953553
2215	10	-0.7978990694911303
2215	11	-0.6822469491326402
2215	12	-0.704057368463431
2215	13	-0.7091291012119854
2215	14	-0.8632803019626465
2215	15	-0.8279398875779052
2215	16	-0.8722461621682953
2215	17	-0.8783435355302274
2215	18	-0.8579166717481073
2215	19	-0.9132566254357428
2215	20	-0.8860373316513233
2215	21	-0.831356120684255
2215	22	-0.8906995623933085
2215	23	-0.7982970078744103
2215	24	-0.9046457155650316
2215	25	-0.8105293965631262
2216	1	-0.631697635215211
2216	2	-0.7991385365486501
2216	3	-0.7373917549139719
2216	4	-0.6243043793120222
2216	5	-0.7035041106982121
2216	6	-0.6230118525437405
2216	7	-0.6653495576256816
2216	8	-0.639924673997335
2216	9	-0.6722859752413157
2216	10	-0.74571985253327
2216	11	-0.6185413352873113
2216	12	-0.6751764394028967
2216	13	-0.6579044911855547
2216	14	-0.7105843674341443
2216	15	-0.6718211591738594
2216	16	-0.8791499737785884
2216	17	-0.9533052152601869
2216	18	-0.8549892289784111
2216	19	-0.8233857878899613
2216	20	-0.7600044260384351
2216	21	-0.8174175238213124
2216	22	-0.8089885459118094
2216	23	-0.9411578474111622
2216	24	-1.075384904246377
2216	25	-1.0179566784731193
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	209017.0
3	6.0
4	1712.0
5	9789.0
6	14389.0
7	14910.0
8	12487.0
9	11495.0
10	11748.0
11	13793.0
12	22833.0
13	20659.0
14	9424.0
15	9141.0
16	9380.0
17	9833.0
18	11259.0
19	13675.0
20	17187.0
21	21540.0
22	26662.0
23	32470.0
24	38349.0
25	44482.0
26	50639.0
27	57268.0
28	66491.0
29	80963.0
30	103305.0
31	133634.0
32	172720.0
33	231351.0
34	321369.0
35	454745.0
36	686159.0
37	1274738.0
38	4240386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63148972841927	17.825602125105654	12.979575215623884	25.563332930851203
2	16.85356368830803	20.818094042276343	37.34419278968565	24.984149479729982
3	18.02921855896554	24.59074183168097	28.755538056654995	28.6245015526985
4	12.86657763283065	16.670902801202196	37.03546224952719	33.427057316439964
5	15.758305190365757	35.14047166970212	32.71286759598758	16.388355543944538
6	32.674295021815155	36.78135222619865	16.26488573140359	14.2794670205826
7	29.26878654009369	30.88200299558988	21.370212709589577	18.47899775472685
8	27.019424445966262	31.760015896276794	20.09302397097037	21.127535686786576
9	27.708181708608237	14.843213380802888	18.939680239349126	38.50892467123975
10	16.680488295617497	25.767254177568127	30.18693442657273	27.365323100241646
11	36.344931381732245	22.18545813688034	22.333890171723052	19.135720309664368
12	25.439203179665377	23.925970440535973	27.836209153437895	22.798617226360758
13	29.22109440829514	20.355698701016816	24.645040865355725	25.77816602533232
14	24.34317443684304	20.516035876687067	24.83453744560492	30.30625224086498
15	24.94679564864327	26.929553202447376	22.774401650370184	25.349249498539173
16	25.39868927757461	26.327017497759986	23.286143374977552	24.98814984968785
17	24.984857734098238	25.247648932663623	24.417531471518856	25.349961861719283
18	26.70713376993851	23.809937508256265	25.318022399916217	24.16490632188901
19	27.344742937603	24.573312026900563	24.4861298740369	23.595815161459537
20	26.3681139681809	24.367989245266898	24.22719992535667	25.036696861195534
21	26.7708600205791	24.613755107738037	24.22243802738113	24.392946844301736
22	25.59570622896915	24.418960402949796	25.020845657171613	24.964487710909438
23	24.304410115243837	23.729017843704376	25.37795217224265	26.588619868809133
24	24.8771659646644	25.328175202250307	24.786945078184097	25.007713754901197
25	24.88777248369774	24.529565382523064	24.974513216717618	25.60814891706158
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6169.0
1	6169.0
2	5852.0
3	5535.0
4	5535.0
5	5535.0
6	9635.5
7	13736.0
8	13736.0
9	13736.0
10	18572.0
11	23408.0
12	23408.0
13	23408.0
14	31694.5
15	39981.0
16	39981.0
17	39981.0
18	62602.5
19	85224.0
20	85224.0
21	85224.0
22	123548.5
23	161873.0
24	161873.0
25	161873.0
26	222030.5
27	282188.0
28	282188.0
29	282188.0
30	332055.0
31	381922.0
32	381922.0
33	381922.0
34	474929.0
35	567936.0
36	567936.0
37	567936.0
38	627874.5
39	687813.0
40	687813.0
41	687813.0
42	774670.0
43	861527.0
44	861527.0
45	861527.0
46	927950.0
47	994373.0
48	994373.0
49	994373.0
50	1022393.5
51	1050414.0
52	1050414.0
53	1050414.0
54	978087.0
55	905760.0
56	905760.0
57	905760.0
58	848043.0
59	790326.0
60	790326.0
61	790326.0
62	703365.0
63	616404.0
64	616404.0
65	616404.0
66	518241.0
67	420078.0
68	420078.0
69	420078.0
70	327247.0
71	234416.0
72	234416.0
73	234416.0
74	192069.0
75	149722.0
76	149722.0
77	149722.0
78	125195.5
79	100669.0
80	100669.0
81	100669.0
82	74275.0
83	47881.0
84	47881.0
85	47881.0
86	35671.5
87	23462.0
88	23462.0
89	23462.0
90	15889.5
91	8317.0
92	8317.0
93	8317.0
94	4496.0
95	675.0
96	675.0
97	675.0
98	437.0
99	199.0
100	199.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14690293437074764
2	0.1644561092613624
3	0.17769486742802135
4	0.1982976848248843
5	0.18819131140301523
6	0.0711937861051668
7	0.058061410816632804
8	0.06144202227704749
9	0.06793137784266871
10	0.06934981621766788
11	0.7116305327370849
12	0.6691246627662764
13	0.6670797474423191
14	0.6671979506402358
15	0.6685572874162766
16	0.6756612996110642
17	0.6664059892141946
18	0.6772806834225216
19	0.6727180399829409
20	0.678273590285021
21	0.6659213561027366
22	0.6687818734923182
23	0.653746426717327
24	0.6641719487735709
25	0.6578244370454496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8460008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.170095705638694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2623467501531	35.51273382174546
2	11.223398298095951	9.690303573426092
3	2.6739136076679335	3.4629931905490303
4	1.061843053667197	1.8335946500472216
5	0.5807140003065223	1.2534739485418434
6	0.36247306041811517	0.9388798025379473
7	0.25331273530878157	0.7654874518716056
8	0.18028452719764917	0.622632023469467
9	0.15529131769583046	0.6033546942465371
>10	1.0107713356291437	8.513682895344084
>50	0.11122880521744873	3.3324748794922603
>100	0.0920647523658111	8.278140343182008
>500	0.014705726904377324	4.409354893914254
>1k	0.01561419758926615	14.085657739412948
>5k	0.0017349108421929678	5.0618344910923305
>10k+	3.0292094070035944E-4	1.6354016011269248
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	16856	0.19924331040821708	No Hit
GTATCAACGCAGAGTACTTTTTTTT	15674	0.18527169241447525	No Hit
GTATCTGATCGTCTTCGAACCTCCG	13521	0.15982254390303177	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	12639	0.14939702184678785	No Hit
GAATAGGACCGCGGTTCTATTTTGT	12577	0.14866416201970495	No Hit
CTATTGGAGCTGGAATTACCGCGGC	12353	0.14601641038637314	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	11786	0.13931428906450205	No Hit
GTCCTATTCCATTATTCCTAGCTGC	11626	0.13742303789783653	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	10280	0.12151288745826244	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	10241	0.12105189498638773	No Hit
ATCAGATACCGTCGTAGTTCCGACC	10011	0.11833322143430597	No Hit
GAACTACGACGGTATCTGATCGTCT	9935	0.11743487713013984	No Hit
CTTTAATATACGCTATTGGAGCTGG	9920	0.11725757233326493	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	9816	0.11602825907493233	No Hit
GCTTTGAACACTCTAATTTTTTCAA	9268	0.10955072382910276	No Hit
GTATCAACGCAGAGTACGGGCGGTG	9218	0.10895970783951978	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	9126	0.10787223841868708	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	8939	0.10566183861764669	No Hit
TCGTAGTTCCGACCATAAACGATGC	8931	0.10556727605931342	No Hit
CTTATGACCCGCACTTACTGGGAAT	8606	0.10172567212702399	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8470	0.10011810863535825	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.1820319791659772E-5	0.0	0.0	0.0
6	0.0	1.1820319791659772E-5	0.0	0.0	1.1820319791659772E-5
7	0.0	1.1820319791659772E-5	0.0	0.0	1.1820319791659772E-5
8	0.0	1.1820319791659772E-5	0.0	0.0	1.1820319791659772E-5
9	0.0	1.1820319791659772E-5	0.0	0.0	2.3640639583319544E-5
10	0.0	1.1820319791659772E-5	0.0	1.1820319791659772E-5	2.3640639583319544E-5
11	0.0	1.1820319791659772E-5	0.0	2.3640639583319544E-5	2.3640639583319544E-5
12	1.1820319791659772E-5	1.1820319791659772E-5	0.0	2.3640639583319544E-5	8.27422385416184E-5
13	1.1820319791659772E-5	1.1820319791659772E-5	0.0	2.3640639583319544E-5	9.456255833327818E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5145	0.0	16.02628	1
GTATCAA	18325	0.0	13.68995	1
ACGCTTA	425	0.0	12.304645	17
TAGATCG	420	0.0	12.000146	5
CGAACGA	1035	0.0	11.759272	16
CTCGACG	455	0.0	11.4941025	13
CGGTTCT	2350	0.0	11.409965	12
CGCTTAC	460	0.0	11.368557	18
CGCGCCA	570	0.0	11.345198	10
TCGACGC	450	0.0	11.198869	14
CGACGCT	475	0.0	11.009812	15
CGTCGTA	1650	0.0	11.008482	10
AACCGCG	1265	0.0	10.900079	7
AGGCCCG	2260	0.0	10.814406	10
CGAGCCG	2155	0.0	10.67773	15
TAGGCCG	90	9.429088E-5	10.566165	5
GCTCGTA	1010	0.0	10.545682	9
ACTCTAA	1950	0.0	10.436555	10
GCGCGCT	875	0.0	10.432154	15
CGGTCCA	1925	0.0	10.374485	10
>>END_MODULE