FastQCFastQC Report
Thu 2 Feb 2017
SRR4050247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8460008
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT264240.3123401301748178No Hit
TATCAACGCAGAGTACTTTTTTTTT164010.1938650649030119No Hit
GTACAAAGGGCAGGGACTTAATCAA163620.1934040724311372No Hit
GATTAAGAGGGACGGCCGGGGGCAT135540.16021261445615653No Hit
GAATAGGACCGCGGTTCTATTTTGT133140.1573757377061582No Hit
CTATTGGAGCTGGAATTACCGCGGC122910.14528355055929026No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG119630.14140648566762584No Hit
GTATCTGATCGTCTTCGAACCTCCG118970.1406263445613763No Hit
ATACAGGACTCTTTCGAGGCCCTGT108530.1282859306988835No Hit
ATATTAAAGTTGCTGCAGTTAAAAA107770.12738758639471737No Hit
ATCAGATACCGTCGTAGTTCCGACC107100.12659562496867616No Hit
GTCCTATTCCATTATTCCTAGCTGC105270.12443250644680241No Hit
TCGTAGTTCCGACCATAAACGATGC99760.11791951024159787No Hit
CTTTAATATACGCTATTGGAGCTGG99710.11786040864263958No Hit
GGTATCAACGCAGAGTACTTTTTTT98800.11678475954159855No Hit
CTCTTAATCATGGCCTCAGTTCCGA97030.11469256293847477No Hit
GAACTACGACGGTATCTGATCGTCT96200.113711476395767No Hit
GCTTTGAACACTCTAATTTTTTCAA93420.11042542749368559No Hit
CTTATGACCCGCACTTACTGGGAAT87920.1039242516082727No Hit
GTATCAACGCAGAGTACGGGCGGTG87390.10329777465931474No Hit
GAATAATTGCAATCCCCGATCCCCA85620.10120557805619096No Hit
GAATAACGCCGCCGCATCGCCAGTC84910.10036633535098312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58600.016.357761
GTATCAA189700.013.3276211
GCTCGTA11350.010.9596129
ACGCTTA5300.010.923985517
CGCTTAC5400.010.89745518
CGAGCCG23100.010.8883915
CTCGACG5150.010.87388713
CGTCGTA19000.010.84458810
CGAACGA9900.010.83360516
CGGTTCT25750.010.80316512
CGACGCT5400.010.72175315
TCGACGC5500.010.69938314
AGGCCCG24000.010.68216910
CGACCAT18200.010.64303910
GCGCGCT8050.010.61149115
GGTTCTA26900.010.51487313
GCGTTAT7300.010.4317951
CCGTCGT20050.010.4190479
AAGCTCG12050.010.4026787
GCCGCCT25250.010.29950518
Produced by FastQC (version 0.11.5)