FastQCFastQC Report
Thu 2 Feb 2017
SRR4050246_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050246_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711240
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA466676.561357628929756No Hit
CTTCCGTACTCTGCGTTGATACCAC294114.13517237500703No Hit
GAGTACGGAAGCAGTGGTATCAACG231823.259377987739722No Hit
TCCGTACTCTGCGTTGATACCACTG226353.18247005230302No Hit
GCTTCCGTACTCTGCGTTGATACCA179312.5210899274506495No Hit
GTACATGGGAAGCAGTGGTATCAAC157432.2134581857038413No Hit
CCCATGTACTCTGCGTTGATACCAC145232.0419267757718913No Hit
GCGTTGATACCACTGCTTCCGTACT117281.6489511276081212No Hit
GTACATGGAAGCAGTGGTATCAACG104371.467437152016197No Hit
GTATCAACGCAGAGTACGGAAGCAG104041.462797367977054No Hit
TCCATGTACTCTGCGTTGATACCAC88991.2511950958888702No Hit
ACTCTGCGTTGATACCACTGCTTCC78021.096957426466453No Hit
TATCAACGCAGAGTACGGAAGCAGT74011.04057702041505No Hit
CAGTGGTATCAACGCAGAGTACGGA55010.7734379393734886No Hit
CATGTACTCTGCGTTGATACCACTG54610.7678139587199819No Hit
GAGTACATGGGAAGCAGTGGTATCA53310.7495360215960857No Hit
ATACCACTGCTTCCGTACTCTGCGT52030.7315392835048647No Hit
GGTATCAACGCAGAGTACGGAAGCA50280.7069343681457735No Hit
ACGCAGAGTACGGAAGCAGTGGTAT47450.6671447050222147No Hit
GTATCAACGCAGAGTACATGGGAAG39810.5597266745402396No Hit
GCGTTGATACCACTGCTTCCCATGT39670.5577582813115123No Hit
GTACTCTGCGTTGATACCACTGCTT39360.5533996963050447No Hit
AAGCAGTGGTATCAACGCAGAGTAC38620.5429953320960577No Hit
GAAGCAGTGGTATCAACGCAGAGTA36800.5174062201226028No Hit
GTGGTATCAACGCAGAGTACGGAAG35690.5017996738091222No Hit
GAGTACATGGAAGCAGTGGTATCAA33320.4684775884370958No Hit
GTACGGGAAGCAGTGGTATCAACGC30020.4220797480456668No Hit
TATCAACGCAGAGTACATGGGAAGC28940.406895000281199No Hit
GATACCACTGCTTCCGTACTCTGCG28560.40155221866036783No Hit
GTTGATACCACTGCTTCCGTACTCT27660.3888982621899781No Hit
CCACTGCTTCCGTACTCTGCGTTGA26810.3769473033012766No Hit
GCTTCCCATGTACTCTGCGTTGATA26420.37146392216410773No Hit
ATCAACGCAGAGTACGGAAGCAGTG26380.3709015240987571No Hit
CAGTGGTATCAACGCAGAGTACATG24690.34714020583769195No Hit
ACGCAGAGTACATGGGAAGCAGTGG23900.3360328440470165No Hit
CCCGTACTCTGCGTTGATACCACTG23360.32844047016478267No Hit
TTCCCGTACTCTGCGTTGATACCAC23260.32703447500140603No Hit
CGGAAGCAGTGGTATCAACGCAGAG22150.3114279286879253No Hit
CTGCTTCCGTACTCTGCGTTGATAC21720.30538214948540576No Hit
GCTTCCATGTACTCTGCGTTGATAC20970.294837185760081No Hit
CATGGAAGCAGTGGTATCAACGCAG20960.29469658624374334No Hit
AAAAAGTACTCTGCGTTGATACCAC19810.27852764186491197No Hit
GCGTTGATACCACTGCTTCCATGTA19260.2707946684663405No Hit
GGTATCAACGCAGAGTACATGGGAA18270.25687531634891175No Hit
GCAGAGTACGGAAGCAGTGGTATCA17310.24337776278049603No Hit
GTATCAACGCAGAGTACATGGAAGC16960.2384567797086778No Hit
CACTGCTTCCGTACTCTGCGTTGAT16270.228755413081379No Hit
GAGTACGGGAAGCAGTGGTATCAAC16140.22692761936898936No Hit
CTTCCCGTACTCTGCGTTGATACCA15910.22369383049322308No Hit
ATACCACTGCTTCCCATGTACTCTG15670.22031944210111915No Hit
GCAGTGGTATCAACGCAGAGTACGG15490.2177886508070412No Hit
CCATGTACTCTGCGTTGATACCACT15210.2138518643495866No Hit
TGATACCACTGCTTCCGTACTCTGC14720.2069624880490411No Hit
GTATCAACGCAGAGTACGGGAAGCA14370.20204150497722287No Hit
GGGAAGCAGTGGTATCAACGCAGAG14180.19937011416680728No Hit
CTGCTTCCCATGTACTCTGCGTTGA13470.18938754850683315No Hit
TATCAACGCAGAGTACATGGAAGCA13380.18812215285979417No Hit
TACCACTGCTTCCGTACTCTGCGTT13320.18727855576176816No Hit
AAAGTACTCTGCGTTGATACCACTG13100.18418536640233957No Hit
TACCACTGCTTCCCATGTACTCTGC12500.17574939542207973No Hit
GCGTTGATACCACTGCTTCCCGTAC11930.1677352229908329No Hit
CTGCGTTGATACCACTGCTTCCGTA11870.16689162589280693No Hit
CATGGGAAGCAGTGGTATCAACGCA11630.16351723750070302No Hit
GTATCAACGCAGAGTACTTTTTTTT11280.15859625442888478No Hit
GTGGTATCAACGCAGAGTACATGGG10940.1538158708734042No Hit
ACGCAGAGTACATGGAAGCAGTGGT10880.15297227377537823No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10330.14523930037680668No Hit
GCAGTGGTATCAACGCAGAGTACAT10220.14369270569709242No Hit
CCCCATGTACTCTGCGTTGATACCA10090.14186491198470277No Hit
GCCCATGTACTCTGCGTTGATACCA9910.1393341206906248No Hit
TCAACGCAGAGTACGGAAGCAGTGG9880.13891232214161184No Hit
GCAGAGTACATGGGAAGCAGTGGTA9710.13652213036387156No Hit
TCCCATGTACTCTGCGTTGATACCA9540.13413193858613126No Hit
CTTCCCATGTACTCTGCGTTGATAC9320.13103874922670264No Hit
GTACGGGAGCAGTGGTATCAACGCA9160.12878915696530002No Hit
ATACCACTGCTTCCATGTACTCTGC9050.12724256228558573No Hit
CTCTGCGTTGATACCACTGCTTCCG8930.12555536808953377No Hit
GATACCACTGCTTCCCATGTACTCT8860.12457117147517012No Hit
TACGGAAGCAGTGGTATCAACGCAG8820.12400877340981947No Hit
TATCAACGCAGAGTACGGGAAGCAG8650.12161858163207918No Hit
CCACTGCTTCCCATGTACTCTGCGT8600.12091558405039086No Hit
ACGGAAGCAGTGGTATCAACGCAGA8420.11838479275631292No Hit
GCTTCCCGTACTCTGCGTTGATACC8380.11782239469096227No Hit
GTTGATACCACTGCTTCCCATGTAC8180.11501040436420898No Hit
GGTATCAACGCAGAGTACATGGAAG8030.11290141161914403No Hit
ATCAACGCAGAGTACATGGGAAGCA7770.10924582419436478No Hit
ACATGGGAAGCAGTGGTATCAACGC7670.10783982903098813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATTA453.4933146E-516.9119768
CACTCTA350.002148925816.3079789
CTACGGA350.002214791516.2344461
GCACGCC601.4497527E-615.848248515
ACGCCCC601.4508223E-615.84712817
AGGCCCG859.968062E-1015.66844910
TGGGAGT456.686264E-414.7979796
AACACTC456.686264E-414.7979797
TGGGAGG653.3184642E-614.6353656
CACGCCC653.3355682E-614.62811816
ACTCTAA400.00522773914.2694810
GTATTAG400.00538686314.205141
GTCCAGG400.00538686314.205141
GCCGCCT954.829417E-914.01120518
CGCATCG759.549331E-713.94645813
CCGCATC759.549331E-713.94645812
GCATCGC759.549331E-713.94645814
TCGCCAG759.556952E-713.94547217
ATCGCCA759.556952E-713.94547216
GCCCCCC707.1894992E-613.58229219
Produced by FastQC (version 0.11.5)