##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050246_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	711240
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62263933412069	31.0	31.0	34.0	27.0	34.0
2	30.712843765817446	31.0	31.0	34.0	27.0	34.0
3	30.861408244755637	31.0	31.0	34.0	27.0	34.0
4	34.32523480119228	37.0	35.0	37.0	30.0	37.0
5	34.32671250210899	37.0	35.0	37.0	30.0	37.0
6	34.335283448624935	37.0	35.0	37.0	30.0	37.0
7	34.203690737303866	37.0	35.0	37.0	30.0	37.0
8	34.13565463134807	37.0	35.0	37.0	30.0	37.0
9	35.59653984590293	39.0	37.0	39.0	30.0	39.0
10	35.59552049940948	39.0	37.0	39.0	30.0	39.0
11	35.421021033687644	39.0	37.0	39.0	30.0	39.0
12	35.39270007311175	39.0	37.0	39.0	30.0	39.0
13	35.33734463753445	39.0	37.0	39.0	30.0	39.0
14	36.53317023789438	40.0	37.0	41.0	30.0	41.0
15	36.465187559754796	40.0	37.0	41.0	30.0	41.0
16	36.32908160395928	40.0	37.0	41.0	29.0	41.0
17	36.413565041336255	40.0	37.0	41.0	30.0	41.0
18	36.32191102862606	40.0	37.0	41.0	30.0	41.0
19	36.3786471514538	40.0	37.0	41.0	29.0	41.0
20	36.39316686350599	40.0	37.0	41.0	29.0	41.0
21	36.338330240143975	40.0	37.0	41.0	29.0	41.0
22	36.31453236600866	40.0	37.0	41.0	30.0	41.0
23	36.210231426803894	40.0	37.0	41.0	29.0	41.0
24	36.115480006748776	40.0	37.0	41.0	29.0	41.0
25	35.87756594117316	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8375287493331456
1101	2	0.7903499285604134
1101	3	0.7513277210325313
1101	4	0.7116881081947497
1101	5	0.8313236775497117
1101	6	0.7088784194882933
1101	7	0.8000815719335179
1101	8	0.7218083392051184
1101	9	0.9383834618068505
1101	10	0.8635298829499902
1101	11	0.5307336394103928
1101	12	0.7933403098296736
1101	13	0.8393755520799431
1101	14	1.15706635220603
1101	15	1.2092462973557545
1101	16	1.3507273345211956
1101	17	1.337691936659347
1101	18	1.3478235999074144
1101	19	1.3272606271661544
1101	20	1.444634520273219
1101	21	1.2663818119617147
1101	22	1.4615504909442123
1101	23	1.3701730822699218
1101	24	1.4041489543652332
1101	25	1.4164924306277413
1102	1	0.5448781301488772
1102	2	0.621511024760224
1102	3	0.5629019419277768
1102	4	0.5504449439736234
1102	5	0.6367756704644805
1102	6	0.6142778146137147
1102	7	0.5247727532953377
1102	8	0.5918434559644723
1102	9	0.5514319677975834
1102	10	0.6884991364481294
1102	11	0.6161605710255316
1102	12	0.6426592458073301
1102	13	0.5811010689692466
1102	14	0.769401307304534
1102	15	0.7788764884868371
1102	16	0.8195747467873105
1102	17	0.6639451950150459
1102	18	0.8469166747896537
1102	19	0.8819139845294544
1102	20	0.7288153483790083
1102	21	0.8111034691014822
1102	22	0.7619573503147379
1102	23	0.7977099587347283
1102	24	0.8860916691846583
1102	25	0.9356519488938702
1103	1	0.6538307812007353
1103	2	0.569974897680968
1103	3	0.5297599715266017
1103	4	0.5545117247955389
1103	5	0.6514470409390398
1103	6	0.5176497263064732
1103	7	0.39926249461138497
1103	8	0.4390066470114391
1103	9	0.5813938990598118
1103	10	0.6224579029598729
1103	11	0.6424207379775879
1103	12	0.6660011917466662
1103	13	0.6993874406668965
1103	14	0.6466831382732252
1103	15	0.7754518309525906
1103	16	0.7606828681180318
1103	17	0.7790171281119171
1103	18	0.7938077278718438
1103	19	0.8979899996957954
1103	20	0.809594840184289
1103	21	0.9054898591455114
1103	22	0.9043424561121967
1103	23	0.8005746694734768
1103	24	0.8831084714106865
1103	25	0.8353373849261629
1104	1	0.42603180428415044
1104	2	0.4095559081076807
1104	3	0.466440676083014
1104	4	0.48991734196043524
1104	5	0.6031943061873548
1104	6	0.4844767363112936
1104	7	0.5542628336301902
1104	8	0.3283075902370882
1104	9	0.5578038405979058
1104	10	0.6656479569015303
1104	11	0.5777594931005368
1104	12	0.6149506101410722
1104	13	0.5656134456693067
1104	14	0.6074191104284807
1104	15	0.6132049649930948
1104	16	0.7932588083301795
1104	17	0.7649600900815443
1104	18	0.6782795365748342
1104	19	0.8134123417883714
1104	20	0.6930260495020306
1104	21	0.7295099064574657
1104	22	0.7624564945041925
1104	23	0.651616398921405
1104	24	0.6667946982067363
1104	25	0.916446692122399
1105	1	0.16007486264012982
1105	2	0.0948624450294524
1105	3	0.09610703592053937
1105	4	0.15252308843190576
1105	5	0.3322294746511645
1105	6	0.08482538918526217
1105	7	0.12999757983865834
1105	8	0.025950302314470264
1105	9	0.0728119861810228
1105	10	0.16231940864169303
1105	11	0.3426160504775879
1105	12	0.3030158697769707
1105	13	0.3567915599850764
1105	14	0.34773664206110055
1105	15	0.21863364913441075
1105	16	0.4907965044816649
1105	17	0.43494160727858144
1105	18	0.44964342862942175
1105	19	0.5096140527260999
1105	20	0.631475758744898
1105	21	0.6852306261909646
1105	22	0.7502527307334077
1105	23	0.6981539781856014
1105	24	0.7109671361834131
1105	25	0.9107694398504051
1106	1	0.2609519605977795
1106	2	0.27314551404178644
1106	3	0.3897326389479545
1106	4	0.36380927007675723
1106	5	0.2614820817517156
1106	6	0.2555679073121908
1106	7	0.38506463504274535
1106	8	0.3675192929837934
1106	9	0.3404631763477042
1106	10	0.299124482738236
1106	11	0.46699045467205735
1106	12	0.524145480950672
1106	13	0.5054964954214256
1106	14	0.6941334629872387
1106	15	0.7065767117914845
1106	16	0.6440539358015371
1106	17	0.5912611179236436
1106	18	0.6456029644066419
1106	19	0.7367612997434634
1106	20	0.6527387942476821
1106	21	0.6969923689763036
1106	22	0.5525500124158214
1106	23	0.41839800568653374
1106	24	0.48977162530725593
1106	25	0.4902291345091001
1107	1	0.4352392422856397
1107	2	0.5265233941432328
1107	3	0.46119422860207493
1107	4	0.46659898894647966
1107	5	0.6004041458918721
1107	6	0.7661682994196823
1107	7	0.48427502409251844
1107	8	0.5431708569171008
1107	9	0.6353370004749053
1107	10	0.5631234395164739
1107	11	0.8009886919870226
1107	12	0.705056321463644
1107	13	0.7814592272235004
1107	14	0.9169440462920946
1107	15	0.9443197554808904
1107	16	0.7612724907595378
1107	17	0.9751145632534275
1107	18	1.043815098154866
1107	19	0.9383054478090003
1107	20	1.0482925111748571
1107	21	0.9322181905134386
1107	22	0.9499239714423879
1107	23	1.1172720456527188
1107	24	1.1624679938163496
1107	25	1.1783687823318232
1108	1	-0.06516152378046769
1108	2	0.0968560654441255
1108	3	-0.030786903473398297
1108	4	-0.0938670346029582
1108	5	0.07144968426610632
1108	6	-0.05385227087397482
1108	7	-0.24587818912921477
1108	8	-0.10045235204871261
1108	9	0.06863250338312099
1108	10	-0.08325902608147828
1108	11	-0.12942342320662448
1108	12	-0.10739225280220666
1108	13	-0.15232925341204862
1108	14	-0.15816589838090778
1108	15	-0.222668469799288
1108	16	0.07106820646389878
1108	17	-0.10249573607980977
1108	18	-0.07606715464695668
1108	19	-0.16536173902601092
1108	20	-0.033325448819468306
1108	21	-0.0425761065499799
1108	22	-0.24288351898179172
1108	23	-0.08706190242502032
1108	24	-0.06329807229231932
1108	25	-0.08893863716030381
1109	1	0.12589574267333958
1109	2	0.11720574100288417
1109	3	0.20106241159509608
1109	4	0.34313900790056096
1109	5	0.3014853899687182
1109	6	0.2735733136352394
1109	7	0.48589473633512625
1109	8	0.30789042269636724
1109	9	0.5544978516853831
1109	10	0.4128305128343044
1109	11	0.6134208449981386
1109	12	0.5215894480252032
1109	13	0.5396137891258022
1109	14	0.4979727358759618
1109	15	0.4670043423681136
1109	16	0.6879374343280773
1109	17	0.5963000547671697
1109	18	0.45878882090837436
1109	19	0.6009616035770762
1109	20	0.5303809061089453
1109	21	0.5693552629697152
1109	22	0.7188392811692736
1109	23	0.6292820396218772
1109	24	0.7609775139335184
1109	25	0.8535906312161146
1110	1	-0.10587220970938205
1110	2	-0.04321548878018788
1110	3	-0.15781942379860325
1110	4	-0.01867312163626167
1110	5	0.028824489843735535
1110	6	-0.26716247728431597
1110	7	-0.36204428186783844
1110	8	-0.4090218788467368
1110	9	-0.5303717823990866
1110	10	-0.3707732044814094
1110	11	-0.12607749062448903
1110	12	-0.1813321839308415
1110	13	-0.4023470825377089
1110	14	-0.34278904160482426
1110	15	-0.11411456471136461
1110	16	-0.17227219059922305
1110	17	-0.2340398742028995
1110	18	-0.021467579829149486
1110	19	-0.06105965590492701
1110	20	-0.11849857811968434
1110	21	-0.16523285003470534
1110	22	-0.13632647596774916
1110	23	-0.3484759388711467
1110	24	-0.08349167961686277
1110	25	-0.09703985225766587
1111	1	0.05993981652682123
1111	2	0.01849222105053272
1111	3	0.20551744435269015
1111	4	0.1291584639259753
1111	5	0.13982170126512727
1111	6	0.07460964478473642
1111	7	0.10352030711138838
1111	8	-0.07277664103203563
1111	9	0.08933207840763657
1111	10	0.16495280785117927
1111	11	0.2735399635210669
1111	12	0.29982252326840353
1111	13	0.29154946512341695
1111	14	0.24291615457757132
1111	15	0.2767561787786761
1111	16	0.19475895507455476
1111	17	0.37555754930757246
1111	18	0.12644020069792816
1111	19	0.11805114100014436
1111	20	0.3009586309451606
1111	21	0.12736995425420616
1111	22	0.20139238817741756
1111	23	0.1383549004517377
1111	24	0.11608571325851358
1111	25	0.3267691104696411
1112	1	-0.03860591060960772
1112	2	0.0013932705257957423
1112	3	-0.06958000692167587
1112	4	0.042981552381746724
1112	5	-0.09803437069889043
1112	6	-0.17568270903835526
1112	7	-0.20372107219895952
1112	8	-0.13301652109201
1112	9	0.0643545671632566
1112	10	-0.04961240954012425
1112	11	-0.024840846074134504
1112	12	-0.02264374359815946
1112	13	0.0032061367875826363
1112	14	-0.0010943401750509452
1112	15	0.034072520607765
1112	16	0.11089838535941254
1112	17	-0.010604369732909902
1112	18	-0.09227794023160385
1112	19	7.647841785569653E-4
1112	20	0.16808756647738932
1112	21	0.14814206819723807
1112	22	0.11744186343977958
1112	23	0.07651082249071806
1112	24	0.12162544339344805
1112	25	0.1642503698399551
1113	1	-0.6337576936924272
1113	2	-0.593191168024159
1113	3	-0.48612878381527835
1113	4	-0.5160812239224128
1113	5	-0.5121664072447203
1113	6	-0.7003290397191648
1113	7	-0.7040010604099791
1113	8	-0.6033377707236056
1113	9	-0.6687429866239469
1113	10	-0.5442705291982435
1113	11	-0.5172664281548904
1113	12	-0.5772046041935042
1113	13	-0.7690971346536131
1113	14	-0.5811046555301971
1113	15	-0.5420695365687749
1113	16	-0.6903320891469278
1113	17	-0.6046951448581694
1113	18	-0.742300044564054
1113	19	-0.6769098400477915
1113	20	-0.6778968131276741
1113	21	-0.5895605548502161
1113	22	-0.665928310447633
1113	23	-0.8560709635393451
1113	24	-0.629339779123498
1113	25	-0.5160030931721309
1114	1	-0.4766366401428037
1114	2	-0.2870948833564455
1114	3	-0.41429030483394413
1114	4	-0.3886217786058239
1114	5	-0.2987615581255838
1114	6	-0.48940543950985216
1114	7	-0.328316427582493
1114	8	-0.36128300009740144
1114	9	-0.5774469810422289
1114	10	-0.4222314571591781
1114	11	-0.08319177265166644
1114	12	-0.05252293665469665
1114	13	-0.02294328849976779
1114	14	-0.23406994420976446
1114	15	-0.1361127948977483
1114	16	-0.2191215536506732
1114	17	-0.28232029748332366
1114	18	-0.21646278658393925
1114	19	-0.31743835914774365
1114	20	-0.20630253439053803
1114	21	-0.3384049644089089
1114	22	-0.36918140655787113
1114	23	-0.2585173266999945
1114	24	-0.31562531898047297
1114	25	-0.520642897811932
1115	1	-0.25652473366014306
1115	2	-0.3434008796166701
1115	3	-0.35098083264709246
1115	4	-0.21221993624999413
1115	5	-0.11539495684763779
1115	6	-0.2294551693511977
1115	7	-0.503764684456911
1115	8	-0.1420529809396598
1115	9	-0.13504067619314242
1115	10	-0.23928357311516635
1115	11	-0.18736919404180696
1115	12	-0.03105364067323535
1115	13	-0.15813159653799147
1115	14	-0.002436143978037819
1115	15	-0.08837616230204759
1115	16	-0.20659158382125753
1115	17	-0.11737008468319488
1115	18	-0.13913058894940633
1115	19	-0.32055579499223086
1115	20	-0.18189934984522438
1115	21	-0.24502098349783097
1115	22	-0.24411974614328358
1115	23	-0.23640131334693137
1115	24	-0.33101891318037246
1115	25	-0.29134072162948854
1116	1	-1.1289520799373953
1116	2	-1.1159588024649452
1116	3	-1.2373044132010236
1116	4	-1.2140558822083491
1116	5	-1.0580974325531756
1116	6	-1.1725329706779632
1116	7	-1.3181917551454276
1116	8	-1.1551552571714367
1116	9	-1.2742070858623649
1116	10	-1.308659102147132
1116	11	-1.1289063230632692
1116	12	-1.4879764955101464
1116	13	-1.277677400043217
1116	14	-1.4486825596839736
1116	15	-1.5694898032886542
1116	16	-1.7603044859675734
1116	17	-1.6503534640573463
1116	18	-1.6864983883732947
1116	19	-1.5832751997205463
1116	20	-1.676676287004426
1116	21	-1.756839147420635
1116	22	-1.9695533062905284
1116	23	-2.230888882326134
1116	24	-2.4432511163811412
1116	25	-2.4141207558812283
1201	1	0.6861233914060492
1201	2	0.6804245882002924
1201	3	0.6381744491836052
1201	4	0.6509942127182455
1201	5	0.7538313631008791
1201	6	0.762435731741256
1201	7	0.626322239478533
1201	8	0.6780446150066055
1201	9	0.9359021267168188
1201	10	0.8845663344212227
1201	11	0.8724771615886979
1201	12	0.8855230063601951
1201	13	0.877539803287668
1201	14	1.0473605990220207
1201	15	0.9747271932714341
1201	16	0.9983338343016044
1201	17	1.017489916457329
1201	18	1.0889522779926182
1201	19	0.9741864067006247
1201	20	1.0565141865007135
1201	21	1.0958723697131418
1201	22	1.2264165427667848
1201	23	1.039804175814055
1201	24	1.176568805045953
1201	25	1.2678319124020092
1202	1	0.5142936219423859
1202	2	0.6112418580198664
1202	3	0.46847635782326336
1202	4	0.5668214006305092
1202	5	0.4736647011797075
1202	6	0.4000286248821112
1202	7	0.5572334701762145
1202	8	0.5584542464711646
1202	9	0.6139447033328977
1202	10	0.5276469224834486
1202	11	0.5564544591219871
1202	12	0.5564074724441141
1202	13	0.5494208241393892
1202	14	0.4455573094421865
1202	15	0.41444986631604763
1202	16	0.24650064807873662
1202	17	0.44816197401073765
1202	18	0.42232642261644315
1202	19	0.26607539999049123
1202	20	0.4945949169279018
1202	21	0.4198428337280262
1202	22	0.5717759193980498
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2210	19	-0.05148962464515705
2210	20	-0.0192904712172961
2210	21	0.0736662788511353
2210	22	0.0383512022677337
2210	23	-0.10902035743777105
2210	24	-0.15448661042147194
2210	25	-0.2689900398321825
2211	1	0.1580523529479052
2211	2	0.17061013076408216
2211	3	0.05852989374157147
2211	4	0.040817751775520605
2211	5	0.09760351531893718
2211	6	0.1750743210627803
2211	7	0.09076138631068886
2211	8	-0.0979395426099714
2211	9	0.01073972967338932
2211	10	0.08731100212218479
2211	11	-0.008694872063749415
2211	12	1.7594968835510372E-4
2211	13	-0.05253423715077332
2211	14	0.15310346301212974
2211	15	0.02501666994301388
2211	16	0.03769004827469047
2211	17	-0.083280759997308
2211	18	-0.07941593128829538
2211	19	-0.128680610617522
2211	20	0.050056144851389206
2211	21	0.19829287399537776
2211	22	0.3350201173937464
2211	23	0.15173515549393102
2211	24	0.03101816870180585
2211	25	-0.14733691745634303
2212	1	-0.17785803779277032
2212	2	-0.2931245679575589
2212	3	-0.17505097273746628
2212	4	-0.11974422758541436
2212	5	-0.1584090534873681
2212	6	-0.11861075800088372
2212	7	-0.22262687903580058
2212	8	-0.3311654985513286
2212	9	-0.24442286230382138
2212	10	-0.2765604614881809
2212	11	-0.2724001832886458
2212	12	-0.3248791518680534
2212	13	-0.380519046075527
2212	14	-0.32593760036314023
2212	15	-0.3637256582249009
2212	16	-0.2802532790681127
2212	17	-0.3490951892582146
2212	18	-0.295513497777506
2212	19	-0.28673334987130517
2212	20	-0.28914112437198725
2212	21	-0.2591687244583838
2212	22	-0.2719334164527396
2212	23	-0.2546057620741422
2212	24	-0.29368654347026535
2212	25	-0.16609744326648013
2213	1	-0.39824809294309915
2213	2	-0.514986039819032
2213	3	-0.4914033418295638
2213	4	-0.5334591656154188
2213	5	-0.43281826299931936
2213	6	-0.4517691521181817
2213	7	-0.6268906517927277
2213	8	-0.5552945088104764
2213	9	-0.7493263492278572
2213	10	-0.6520096698140989
2213	11	-0.46089422349501774
2213	12	-0.6554082688697704
2213	13	-0.9376122382714627
2213	14	-0.8833628805623945
2213	15	-0.9146166621980925
2213	16	-1.0171424743477218
2213	17	-0.8858346484291815
2213	18	-0.8020559279500716
2213	19	-0.8708991270165356
2213	20	-0.6706464911513308
2213	21	-0.887037431094214
2213	22	-1.0217999882371203
2213	23	-0.9924074580950162
2213	24	-0.8347987417742431
2213	25	-0.8252335696971258
2214	1	-0.5748361655075627
2214	2	-0.4550791812246189
2214	3	-0.5095125596969368
2214	4	-0.7187603962882889
2214	5	-0.6690101656126117
2214	6	-0.7056200794725243
2214	7	-0.4032874828970847
2214	8	-0.517080289377212
2214	9	-0.49374931908159425
2214	10	-0.5306242639008403
2214	11	-0.7900310706062399
2214	12	-0.8177800979442722
2214	13	-0.5908236159059257
2214	14	-0.8976102968453219
2214	15	-0.9453356372946544
2214	16	-0.8656666238379387
2214	17	-0.7960243819960411
2214	18	-0.9020191005574532
2214	19	-0.769892753267797
2214	20	-0.862550554343656
2214	21	-1.0836406115890327
2214	22	-0.9693832993386948
2214	23	-0.9959307086488778
2214	24	-0.8398661330029427
2214	25	-0.8873015002876414
2215	1	-0.9169785663869838
2215	2	-0.8752617290420908
2215	3	-0.8130550738994629
2215	4	-0.9947651131326154
2215	5	-0.8502295137288769
2215	6	-0.7678247019516746
2215	7	-0.7352265600565318
2215	8	-0.978480756392905
2215	9	-0.9071402618633329
2215	10	-0.9270351330154796
2215	11	-0.971633114100527
2215	12	-0.8999987690929316
2215	13	-0.8099107070461358
2215	14	-1.1086671515137425
2215	15	-1.1303543511693803
2215	16	-1.1242116598685996
2215	17	-1.2554823693296058
2215	18	-1.356560053560905
2215	19	-1.3880016721922601
2215	20	-1.3861867687860538
2215	21	-1.549363909390415
2215	22	-1.7470606284220551
2215	23	-1.7284570569780655
2215	24	-1.6692922243391024
2215	25	-1.6530572670684052
2216	1	-1.1263371233117923
2216	2	-1.1306869012129255
2216	3	-1.2119732777882
2216	4	-1.098764997992248
2216	5	-1.0875828814865685
2216	6	-1.1337748160780237
2216	7	-1.010951110273112
2216	8	-0.8857397679259122
2216	9	-1.351990314428832
2216	10	-1.1555442811141319
2216	11	-1.2616566589977438
2216	12	-1.3561469704498847
2216	13	-1.2247182819740559
2216	14	-1.339904995536486
2216	15	-1.3758794141531254
2216	16	-1.6627460668858802
2216	17	-1.5045813632251992
2216	18	-1.4819040844813287
2216	19	-1.709014331745081
2216	20	-1.6892970634472704
2216	21	-1.7491259090122782
2216	22	-1.7777455369868633
2216	23	-2.0664117060648977
2216	24	-2.2987057477063857
2216	25	-2.1947861651423537
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16679.0
3	1.0
4	130.0
5	999.0
6	1914.0
7	1931.0
8	1709.0
9	1492.0
10	1337.0
11	1913.0
12	2014.0
13	1778.0
14	866.0
15	750.0
16	800.0
17	898.0
18	990.0
19	1353.0
20	1831.0
21	2346.0
22	2946.0
23	3259.0
24	3741.0
25	4265.0
26	4844.0
27	5215.0
28	5811.0
29	6854.0
30	8938.0
31	11221.0
32	14747.0
33	19530.0
34	26990.0
35	39038.0
36	59593.0
37	111245.0
38	341272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.57496034999056	13.328178127843463	13.539316188753764	22.55754533341221
2	9.088838012521586	21.776647747408212	40.39688254121829	28.737631698851906
3	21.840182943808912	31.587341323529827	26.40182381251072	20.17065192015054
4	13.644816168299755	14.052455824970952	31.41708935720093	40.88563864952836
5	17.65946823996006	32.07493302017482	28.90462494813898	21.360973791726146
6	38.72700389827624	27.657221799397043	13.55055449497557	20.065219807351156
7	25.77704242710743	32.63353086503126	22.17560624222676	19.413820465634547
8	26.314812000877946	33.956057561892536	24.445654437297044	15.283475999932467
9	29.833838345473318	21.445713305030147	14.028365082982358	34.69208326651418
10	13.852799239339431	25.05228838150053	29.359697648675525	31.73521473048451
11	30.05417780337235	27.1788914562071	16.54311939067304	26.223811349747507
12	27.76913723383197	23.0587054265758	24.74139637474196	24.43076096485027
13	32.46318550997504	19.223680007237096	27.18943383851662	21.123700644271246
14	33.65443593635611	19.012185504591823	21.88288800133416	25.45049055771791
15	37.10715204491549	16.60668700227009	26.10995360881574	20.17620734399869
16	27.657502847501124	20.34373507358282	33.32819888134894	18.670563197567116
17	25.04271535392621	29.287823050321443	28.133766540275385	17.535695055476964
18	28.127543752543755	16.650556233889567	38.92153371320038	16.3003663003663
19	23.80837957714825	35.1959484481584	18.554394316745867	22.44127765794748
20	18.516068760926743	29.463093224111837	29.617286620224913	22.403551394736507
21	19.57441696113074	34.903038869257955	20.794487632508833	24.728056537102475
22	18.69343651605682	31.314336105097144	16.495904535253782	33.49632284359225
23	23.4784562807934	25.14423253592033	17.787171855080576	33.590139328205694
24	17.235907457570118	30.460776292678933	18.68624566728416	33.61707058246679
25	26.868601225173283	28.601507796878423	17.938402187506625	26.59148879044167
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	251.0
2	361.0
3	471.0
4	471.0
5	471.0
6	1059.0
7	1647.0
8	1647.0
9	1647.0
10	1624.5
11	1602.0
12	1602.0
13	1602.0
14	1820.5
15	2039.0
16	2039.0
17	2039.0
18	3129.5
19	4220.0
20	4220.0
21	4220.0
22	4872.5
23	5525.0
24	5525.0
25	5525.0
26	7491.0
27	9457.0
28	9457.0
29	9457.0
30	11309.5
31	13162.0
32	13162.0
33	13162.0
34	15848.0
35	18534.0
36	18534.0
37	18534.0
38	21283.0
39	24032.0
40	24032.0
41	24032.0
42	31614.0
43	39196.0
44	39196.0
45	39196.0
46	97452.0
47	155708.0
48	155708.0
49	155708.0
50	227514.5
51	299321.0
52	299321.0
53	299321.0
54	182993.5
55	66666.0
56	66666.0
57	66666.0
58	45349.5
59	24033.0
60	24033.0
61	24033.0
62	20684.5
63	17336.0
64	17336.0
65	17336.0
66	14498.0
67	11660.0
68	11660.0
69	11660.0
70	9062.0
71	6464.0
72	6464.0
73	6464.0
74	5366.0
75	4268.0
76	4268.0
77	4268.0
78	3688.0
79	3108.0
80	3108.0
81	3108.0
82	2329.5
83	1551.0
84	1551.0
85	1551.0
86	1139.5
87	728.0
88	728.0
89	728.0
90	481.0
91	234.0
92	234.0
93	234.0
94	127.5
95	21.0
96	21.0
97	21.0
98	13.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17968618187953433
2	0.020808728417974244
3	0.028401102300208085
4	0.04471064619537709
5	0.028822900849221077
6	0.05806760024745515
7	0.06580057364602666
8	0.06917496203813059
9	0.05145942297958495
10	0.0392272650582082
11	0.6317136269051234
12	0.559586075023902
13	0.5306225746583432
14	0.5171250210899274
15	0.5311849727236939
16	0.5064394578482649
17	0.5130476351161352
18	0.5095326472076936
19	0.5283729823969405
20	0.5183904167369665
21	0.5258421911028626
22	0.5100950452730443
23	0.5204994094820314
24	0.4981440863843428
25	0.5040492660705247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	711240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.946514246571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.46100934432106	21.18332820529834
2	5.785830065499919	2.7710092418346526
3	1.5929180059551233	1.1443450116967149
4	0.8568215598219792	0.8207155875618485
5	0.5427411582764632	0.6498379439433886
6	0.3496323921051873	0.5023486235165739
7	0.24640399029341215	0.4130361664781195
8	0.21228775881626363	0.4066841472693028
9	0.17733309905602396	0.3821858624640298
>10	1.358219576258061	6.639663512174565
>50	0.20133706633053886	3.3787309550681357
>100	0.1518858570563714	6.861641914967722
>500	0.022959490020149166	3.8997719535215025
>1k	0.03002394848788737	15.239744333280372
>5k	0.004709638978492137	7.13696471528683
>10k+	0.00588704872311517	28.569991825637896
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	46667	6.561357628929756	No Hit
CTTCCGTACTCTGCGTTGATACCAC	29411	4.13517237500703	No Hit
GAGTACGGAAGCAGTGGTATCAACG	23182	3.259377987739722	No Hit
TCCGTACTCTGCGTTGATACCACTG	22635	3.18247005230302	No Hit
GCTTCCGTACTCTGCGTTGATACCA	17931	2.5210899274506495	No Hit
GTACATGGGAAGCAGTGGTATCAAC	15743	2.2134581857038413	No Hit
CCCATGTACTCTGCGTTGATACCAC	14523	2.0419267757718913	No Hit
GCGTTGATACCACTGCTTCCGTACT	11728	1.6489511276081212	No Hit
GTACATGGAAGCAGTGGTATCAACG	10437	1.467437152016197	No Hit
GTATCAACGCAGAGTACGGAAGCAG	10404	1.462797367977054	No Hit
TCCATGTACTCTGCGTTGATACCAC	8899	1.2511950958888702	No Hit
ACTCTGCGTTGATACCACTGCTTCC	7802	1.096957426466453	No Hit
TATCAACGCAGAGTACGGAAGCAGT	7401	1.04057702041505	No Hit
CAGTGGTATCAACGCAGAGTACGGA	5501	0.7734379393734886	No Hit
CATGTACTCTGCGTTGATACCACTG	5461	0.7678139587199819	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5331	0.7495360215960857	No Hit
ATACCACTGCTTCCGTACTCTGCGT	5203	0.7315392835048647	No Hit
GGTATCAACGCAGAGTACGGAAGCA	5028	0.7069343681457735	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	4745	0.6671447050222147	No Hit
GTATCAACGCAGAGTACATGGGAAG	3981	0.5597266745402396	No Hit
GCGTTGATACCACTGCTTCCCATGT	3967	0.5577582813115123	No Hit
GTACTCTGCGTTGATACCACTGCTT	3936	0.5533996963050447	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3862	0.5429953320960577	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	3680	0.5174062201226028	No Hit
GTGGTATCAACGCAGAGTACGGAAG	3569	0.5017996738091222	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3332	0.4684775884370958	No Hit
GTACGGGAAGCAGTGGTATCAACGC	3002	0.4220797480456668	No Hit
TATCAACGCAGAGTACATGGGAAGC	2894	0.406895000281199	No Hit
GATACCACTGCTTCCGTACTCTGCG	2856	0.40155221866036783	No Hit
GTTGATACCACTGCTTCCGTACTCT	2766	0.3888982621899781	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	2681	0.3769473033012766	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2642	0.37146392216410773	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	2638	0.3709015240987571	No Hit
CAGTGGTATCAACGCAGAGTACATG	2469	0.34714020583769195	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2390	0.3360328440470165	No Hit
CCCGTACTCTGCGTTGATACCACTG	2336	0.32844047016478267	No Hit
TTCCCGTACTCTGCGTTGATACCAC	2326	0.32703447500140603	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2215	0.3114279286879253	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2172	0.30538214948540576	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2097	0.294837185760081	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2096	0.29469658624374334	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1981	0.27852764186491197	No Hit
GCGTTGATACCACTGCTTCCATGTA	1926	0.2707946684663405	No Hit
GGTATCAACGCAGAGTACATGGGAA	1827	0.25687531634891175	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1731	0.24337776278049603	No Hit
GTATCAACGCAGAGTACATGGAAGC	1696	0.2384567797086778	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1627	0.228755413081379	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1614	0.22692761936898936	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1591	0.22369383049322308	No Hit
ATACCACTGCTTCCCATGTACTCTG	1567	0.22031944210111915	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1549	0.2177886508070412	No Hit
CCATGTACTCTGCGTTGATACCACT	1521	0.2138518643495866	No Hit
TGATACCACTGCTTCCGTACTCTGC	1472	0.2069624880490411	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1437	0.20204150497722287	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1418	0.19937011416680728	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1347	0.18938754850683315	No Hit
TATCAACGCAGAGTACATGGAAGCA	1338	0.18812215285979417	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1332	0.18727855576176816	No Hit
AAAGTACTCTGCGTTGATACCACTG	1310	0.18418536640233957	No Hit
TACCACTGCTTCCCATGTACTCTGC	1250	0.17574939542207973	No Hit
GCGTTGATACCACTGCTTCCCGTAC	1193	0.1677352229908329	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1187	0.16689162589280693	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1163	0.16351723750070302	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1128	0.15859625442888478	No Hit
GTGGTATCAACGCAGAGTACATGGG	1094	0.1538158708734042	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1088	0.15297227377537823	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1033	0.14523930037680668	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1022	0.14369270569709242	No Hit
CCCCATGTACTCTGCGTTGATACCA	1009	0.14186491198470277	No Hit
GCCCATGTACTCTGCGTTGATACCA	991	0.1393341206906248	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	988	0.13891232214161184	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	971	0.13652213036387156	No Hit
TCCCATGTACTCTGCGTTGATACCA	954	0.13413193858613126	No Hit
CTTCCCATGTACTCTGCGTTGATAC	932	0.13103874922670264	No Hit
GTACGGGAGCAGTGGTATCAACGCA	916	0.12878915696530002	No Hit
ATACCACTGCTTCCATGTACTCTGC	905	0.12724256228558573	No Hit
CTCTGCGTTGATACCACTGCTTCCG	893	0.12555536808953377	No Hit
GATACCACTGCTTCCCATGTACTCT	886	0.12457117147517012	No Hit
TACGGAAGCAGTGGTATCAACGCAG	882	0.12400877340981947	No Hit
TATCAACGCAGAGTACGGGAAGCAG	865	0.12161858163207918	No Hit
CCACTGCTTCCCATGTACTCTGCGT	860	0.12091558405039086	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	842	0.11838479275631292	No Hit
GCTTCCCGTACTCTGCGTTGATACC	838	0.11782239469096227	No Hit
GTTGATACCACTGCTTCCCATGTAC	818	0.11501040436420898	No Hit
GGTATCAACGCAGAGTACATGGAAG	803	0.11290141161914403	No Hit
ATCAACGCAGAGTACATGGGAAGCA	777	0.10924582419436478	No Hit
ACATGGGAAGCAGTGGTATCAACGC	767	0.10783982903098813	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.811990326753276E-4	0.0
11	0.0	0.0	0.0	8.435970980259828E-4	0.0
12	0.0	0.0	0.0	0.0012653956470389742	0.0
13	0.0	0.0	0.0	0.0015465946797143019	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCATTA	45	3.4933146E-5	16.911976	8
CACTCTA	35	0.0021489258	16.307978	9
CTACGGA	35	0.0022147915	16.234446	1
GCACGCC	60	1.4497527E-6	15.8482485	15
ACGCCCC	60	1.4508223E-6	15.847128	17
AGGCCCG	85	9.968062E-10	15.668449	10
TGGGAGT	45	6.686264E-4	14.797979	6
AACACTC	45	6.686264E-4	14.797979	7
TGGGAGG	65	3.3184642E-6	14.635365	6
CACGCCC	65	3.3355682E-6	14.628118	16
ACTCTAA	40	0.005227739	14.26948	10
GTATTAG	40	0.005386863	14.20514	1
GTCCAGG	40	0.005386863	14.20514	1
GCCGCCT	95	4.829417E-9	14.011205	18
CGCATCG	75	9.549331E-7	13.946458	13
CCGCATC	75	9.549331E-7	13.946458	12
GCATCGC	75	9.549331E-7	13.946458	14
TCGCCAG	75	9.556952E-7	13.945472	17
ATCGCCA	75	9.556952E-7	13.945472	16
GCCCCCC	70	7.1894992E-6	13.582292	19
>>END_MODULE