FastQCFastQC Report
Thu 2 Feb 2017
SRR4050246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711240
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA416195.85161127045723No Hit
CTTCCGTACTCTGCGTTGATACCAC278413.914431134356898No Hit
GAGTACGGAAGCAGTGGTATCAACG238953.359625442888477No Hit
TCCGTACTCTGCGTTGATACCACTG227533.1990607952308645No Hit
GCTTCCGTACTCTGCGTTGATACCA173172.434761824419324No Hit
CCCATGTACTCTGCGTTGATACCAC154112.166779146279737No Hit
GTACATGGGAAGCAGTGGTATCAAC138561.9481468983746697No Hit
GCGTTGATACCACTGCTTCCGTACT114121.6045216804454192No Hit
GTATCAACGCAGAGTACGGAAGCAG103891.4606883752319892No Hit
TCCATGTACTCTGCGTTGATACCAC94661.3309150216523256No Hit
GTACATGGAAGCAGTGGTATCAACG91881.2918283561104549No Hit
TATCAACGCAGAGTACGGAAGCAGT84791.1921432990270513No Hit
ACTCTGCGTTGATACCACTGCTTCC81471.145464259602947No Hit
CAGTGGTATCAACGCAGAGTACGGA63270.8895731398683989No Hit
CATGTACTCTGCGTTGATACCACTG59360.8345987289803724No Hit
ACGCAGAGTACGGAAGCAGTGGTAT56400.7929812721444239No Hit
ATACCACTGCTTCCGTACTCTGCGT52370.7363196670603452No Hit
GAGTACATGGGAAGCAGTGGTATCA51540.7246499072043192No Hit
GGTATCAACGCAGAGTACGGAAGCA51310.7214161183285529No Hit
AAGCAGTGGTATCAACGCAGAGTAC43360.6096395028401101No Hit
GAAGCAGTGGTATCAACGCAGAGTA41030.5768798155334346No Hit
GCGTTGATACCACTGCTTCCCATGT40690.572099431977954No Hit
GTGGTATCAACGCAGAGTACGGAAG40440.5685844440695125No Hit
GTATCAACGCAGAGTACATGGGAAG38080.5354029582138238No Hit
GAGTACATGGAAGCAGTGGTATCAA32790.4610258140711996No Hit
GTACTCTGCGTTGATACCACTGCTT32520.45722962713008264No Hit
TATCAACGCAGAGTACATGGGAAGC32040.45048085034587476No Hit
ATCAACGCAGAGTACGGAAGCAGTG30470.42840672628086157No Hit
GTACGGGAAGCAGTGGTATCAACGC28470.4002868230133288No Hit
GATACCACTGCTTCCGTACTCTGCG28370.3988808278499522No Hit
ACGCAGAGTACATGGGAAGCAGTGG27220.38271188347112084No Hit
CCACTGCTTCCGTACTCTGCGTTGA27210.3825712839547832No Hit
GTTGATACCACTGCTTCCGTACTCT26640.3745571115235363No Hit
GCTTCCCATGTACTCTGCGTTGATA26440.3717451211967831No Hit
CAGTGGTATCAACGCAGAGTACATG25600.35993476182441936No Hit
CGGAAGCAGTGGTATCAACGCAGAG25160.3537483831055621No Hit
CCCGTACTCTGCGTTGATACCACTG24080.3385636353410944No Hit
CATGGAAGCAGTGGTATCAACGCAG23550.33111186097519824No Hit
TTCCCGTACTCTGCGTTGATACCAC22890.32183229289691245No Hit
CTGCTTCCGTACTCTGCGTTGATAC22230.3125527248186266No Hit
AAAAAGTACTCTGCGTTGATACCAC21780.3062257465834317No Hit
GCTTCCATGTACTCTGCGTTGATAC19900.279793037511951No Hit
GCGTTGATACCACTGCTTCCATGTA19600.27557505202182103No Hit
GCAGAGTACGGAAGCAGTGGTATCA19190.2698104718519768No Hit
GCAGTGGTATCAACGCAGAGTACGG17660.24829874585231426No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17410.24478375794387267No Hit
GTATCAACGCAGAGTACTTTTTTTT17360.24408076036218437No Hit
CCATGTACTCTGCGTTGATACCACT17170.24140936955176873No Hit
GGTATCAACGCAGAGTACATGGGAA16980.23873797874135314No Hit
CACTGCTTCCGTACTCTGCGTTGAT16780.23592598841459983No Hit
GAGTACGGGAAGCAGTGGTATCAAC16580.2331139980878466No Hit
GTATCAACGCAGAGTACATGGAAGC16460.2314268038917946No Hit
ATACCACTGCTTCCCATGTACTCTG15440.21708565322535292No Hit
CTTCCCGTACTCTGCGTTGATACCA15240.21427366289859962No Hit
GGGAAGCAGTGGTATCAACGCAGAG14890.20935267982678138No Hit
TGATACCACTGCTTCCGTACTCTGC14650.20597829143467744No Hit
CTGCTTCCCATGTACTCTGCGTTGA14240.20021371126483328No Hit
AAAGTACTCTGCGTTGATACCACTG13860.19487092964400202No Hit
TATCAACGCAGAGTACATGGAAGCA13690.19248073786626174No Hit
CATGGGAAGCAGTGGTATCAACGCA13620.1914965412518981No Hit
TACCACTGCTTCCGTACTCTGCGTT13580.19093414318654744No Hit
GTATCAACGCAGAGTACGGGAAGCA13480.1895281480231708No Hit
TATCAACGCAGAGTACTTTTTTTTT12880.18109217704291097No Hit
CTGCGTTGATACCACTGCTTCCGTA12820.180248579944885No Hit
TCAACGCAGAGTACGGAAGCAGTGG12690.17842078623249535No Hit
ACGCAGAGTACATGGAAGCAGTGGT12660.17799898768348238No Hit
TACCACTGCTTCCCATGTACTCTGC12520.17603059445475508No Hit
GTGGTATCAACGCAGAGTACATGGG12030.16914121815420957No Hit
GCGTTGATACCACTGCTTCCCGTAC11800.16590742927844326No Hit
GCAGTGGTATCAACGCAGAGTACAT11460.16112704572296271No Hit
GCAGAGTACATGGGAAGCAGTGGTA11320.15915865249423541No Hit
CCCCATGTACTCTGCGTTGATACCA11070.15564366458579385No Hit
TCCCATGTACTCTGCGTTGATACCA10570.14861368876891062No Hit
TATCAACGCAGAGTACGGGAAGCAG10550.1483324897362353No Hit
TACGGAAGCAGTGGTATCAACGCAG10480.14734829312187167No Hit
ACGGAAGCAGTGGTATCAACGCAGA10130.14242731005005344No Hit
GCCCATGTACTCTGCGTTGATACCA10070.14158371295202743No Hit
ATACCACTGCTTCCATGTACTCTGC9550.13427253810246895No Hit
CTCTGCGTTGATACCACTGCTTCCG9480.13328834148810528No Hit
GATACCACTGCTTCCCATGTACTCT9330.13117934874304032No Hit
GTTGATACCACTGCTTCCCATGTAC9290.13061695067768966No Hit
CCACTGCTTCCCATGTACTCTGCGT9230.12977335357966369No Hit
GCTTCCCGTACTCTGCGTTGATACC9180.12907035599797537No Hit
ACATGGGAAGCAGTGGTATCAACGC9160.12878915696530002No Hit
CTTCCCATGTACTCTGCGTTGATAC9070.12752376131826107No Hit
GTACGGGAGCAGTGGTATCAACGCA8460.11894719082166356No Hit
ATCAACGCAGAGTACATGGGAAGCA8230.1157134019458973No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7860.11051121984140375No Hit
GGTATCAACGCAGAGTACTTTTTTT7610.10699623193296216No Hit
GGTATCAACGCAGAGTACATGGAAG7340.10320004499184522No Hit
AACGCAGAGTACGGAAGCAGTGGTA7230.10165345031213092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTT250.00604287118.9924037
TTATCAA659.824362E-917.563572
TAGAGTG402.7594846E-416.6241995
AAGCTCG402.7669003E-416.6183537
GCTCGTA350.00217546516.2780579
ATTGACG350.002176487416.27691311
GTTATCA755.567017E-815.257241
CGGTCCA755.8687874E-815.19178610
GACATGG851.679291E-814.5502122
AGGAATT400.005289388414.2443037
GTGGGTA400.005291857314.24339
AGTGTTC400.005291857314.24338
TGACGGA400.005301743314.23929513
CCGACCA954.9167284E-913.9934189
CGACCAT954.9203663E-913.99243410
AGGCCCG759.710475E-713.92580410
ACGATGC1051.3624231E-913.5736419
GGTCCAA852.7098213E-713.40451711
AAGAATT852.7191345E-713.40074716
GCTTACG500.001456300613.3500831
Produced by FastQC (version 0.11.5)