##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	711240
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114175243237163	31.0	31.0	34.0	28.0	34.0
2	30.88965609358304	31.0	31.0	34.0	27.0	34.0
3	31.276155728024296	33.0	31.0	34.0	27.0	34.0
4	34.79694055452449	37.0	35.0	37.0	32.0	37.0
5	34.695441763680336	37.0	35.0	37.0	32.0	37.0
6	34.75204009898206	37.0	35.0	37.0	32.0	37.0
7	34.60749114223047	37.0	35.0	37.0	32.0	37.0
8	34.64074292784433	37.0	35.0	37.0	32.0	37.0
9	36.26489792475114	39.0	37.0	39.0	32.0	39.0
10	36.23463247286429	39.0	37.0	39.0	32.0	39.0
11	36.229821157415216	39.0	37.0	39.0	32.0	39.0
12	36.190075080141725	39.0	37.0	39.0	32.0	39.0
13	36.09567375288229	39.0	37.0	39.0	32.0	39.0
14	37.33858894325404	40.0	38.0	41.0	32.0	41.0
15	37.303304088633936	40.0	38.0	41.0	32.0	41.0
16	37.12637084528429	40.0	37.0	41.0	32.0	41.0
17	37.17785698217198	40.0	38.0	41.0	32.0	41.0
18	37.06910888026545	40.0	37.0	41.0	32.0	41.0
19	37.16979219391485	40.0	38.0	41.0	32.0	41.0
20	37.170343344018896	40.0	38.0	41.0	32.0	41.0
21	37.13654181429616	40.0	38.0	41.0	31.0	41.0
22	37.120912772060066	40.0	38.0	41.0	31.0	41.0
23	37.03482509420168	40.0	37.0	41.0	31.0	41.0
24	36.95939204769135	40.0	37.0	41.0	31.0	41.0
25	36.73383105562117	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8118236763278581
1101	2	1.0041266360834875
1101	3	0.7397696521568946
1101	4	0.7849056922506321
1101	5	0.828632388717871
1101	6	0.7468931790994091
1101	7	0.8974730323335294
1101	8	0.7726872626210266
1101	9	0.837279355162039
1101	10	0.8887076006994974
1101	11	0.8709078361699127
1101	12	1.0091337253778931
1101	13	1.0433626705526393
1101	14	1.059805989119866
1101	15	1.2207069748581745
1101	16	1.0470925405592908
1101	17	1.121559152046224
1101	18	1.0915589081704198
1101	19	1.1328592589875228
1101	20	1.0233156768773881
1101	21	0.8800487668178789
1101	22	1.15747176105328
1101	23	1.2412644535241952
1101	24	1.2255071128007557
1101	25	1.0636368286239204
1102	1	0.28497753259666325
1102	2	0.5310385270513649
1102	3	0.4282863171476521
1102	4	0.32697232127378584
1102	5	0.35333787977237563
1102	6	0.2310732337082939
1102	7	0.31297623074322445
1102	8	0.2978549180745702
1102	9	0.2400836681807661
1102	10	0.2579634193299398
1102	11	0.2669691899441915
1102	12	0.33171480719065727
1102	13	0.35487887918022665
1102	14	0.31173779763297205
1102	15	0.3308007226827172
1102	16	0.3061888641285151
1102	17	0.24639381708339414
1102	18	0.28529087602389325
1102	19	0.2566477694645357
1102	20	0.45732672241314987
1102	21	0.4939790592770592
1102	22	0.6603453020447461
1102	23	0.6697643336321732
1102	24	0.4501344806681473
1102	25	0.5248633626616481
1103	1	0.44627278699979556
1103	2	0.6814750180004836
1103	3	0.5528525017122305
1103	4	0.6235683034166399
1103	5	0.5338070119886211
1103	6	0.5699385101270806
1103	7	0.6216655348038884
1103	8	0.4688450874728076
1103	9	0.5613020206758748
1103	10	0.5284100772311149
1103	11	0.4720110044011392
1103	12	0.6030921616427136
1103	13	0.7309051297917719
1103	14	0.7478618191593895
1103	15	0.6449442525261588
1103	16	0.868728996833994
1103	17	0.7972400438446456
1103	18	0.8968253977949274
1103	19	0.6492794047385075
1103	20	0.7626429681323259
1103	21	0.7807559061459415
1103	22	0.8667726503813427
1103	23	0.9466475130597658
1103	24	1.0185569213481855
1103	25	0.6062620447800455
1104	1	0.41202435402004767
1104	2	0.7035093568384774
1104	3	0.4004833253019129
1104	4	0.4078374542122063
1104	5	0.502328174295755
1104	6	0.43742359329969105
1104	7	0.41493751828990355
1104	8	0.5617936846883325
1104	9	0.7173806278359507
1104	10	0.4479637401409491
1104	11	0.7098053857294886
1104	12	0.5261970465269954
1104	13	0.4839836088780771
1104	14	0.48380383941011473
1104	15	0.5228183786110208
1104	16	0.5547985258238697
1104	17	0.5482250818147492
1104	18	0.5708117315702381
1104	19	0.7040064569323334
1104	20	0.6519542702658825
1104	21	0.6501470283735244
1104	22	0.8665767728500029
1104	23	0.879950271919455
1104	24	0.8069178407671842
1104	25	0.6243251550138922
1105	1	0.4429583930604011
1105	2	0.6567946202732102
1105	3	0.5006803899698049
1105	4	0.43695608750754644
1105	5	0.5272078169128633
1105	6	0.4478977904301118
1105	7	0.5976953643493417
1105	8	0.4414125590637141
1105	9	0.45861499416072604
1105	10	0.4690765071553571
1105	11	0.5656130403859905
1105	12	0.7172611957336201
1105	13	0.729751010473592
1105	14	0.5400019706745383
1105	15	0.5756970934352523
1105	16	0.5795777184249005
1105	17	0.521139191571919
1105	18	0.5510039015828028
1105	19	0.568135938829414
1105	20	0.634358166995959
1105	21	0.6797852622065506
1105	22	0.7706552261389206
1105	23	0.7549749278324924
1105	24	0.7333710785451544
1105	25	0.6754204254618656
1106	1	0.2942679013944556
1106	2	0.6598603105409993
1106	3	0.4392519371864907
1106	4	0.5444583624013859
1106	5	0.3288433933637407
1106	6	0.33400324439781315
1106	7	0.26943089169139967
1106	8	0.48686168810436214
1106	9	0.4941565994413679
1106	10	0.34057028189031513
1106	11	0.32797781058560105
1106	12	0.5147658538495747
1106	13	0.4418063228923472
1106	14	0.6120032633919905
1106	15	0.5121354763107178
1106	16	0.5188245119078729
1106	17	0.6013969045453109
1106	18	0.4486399980094191
1106	19	0.453253799995899
1106	20	0.45035987858275206
1106	21	0.5425044464223987
1106	22	0.730079966873241
1106	23	0.5545432396446088
1106	24	0.6954841660455173
1106	25	0.731970494791959
1107	1	0.5219213719054565
1107	2	0.661125666585388
1107	3	0.5722105500612855
1107	4	0.6161906501147527
1107	5	0.52523168769617
1107	6	0.4508494771082141
1107	7	0.4996357588604923
1107	8	0.4221801405388419
1107	9	0.41991788152493115
1107	10	0.3974069817122441
1107	11	0.5219630975615175
1107	12	0.5335240602276201
1107	13	0.5831493809710153
1107	14	0.5496896493480676
1107	15	0.6260615874318205
1107	16	0.5493783187679568
1107	17	0.6230581452597406
1107	18	0.4324754567571887
1107	19	0.357629935398883
1107	20	0.6122633985568555
1107	21	0.7576869202968837
1107	22	0.575675952268135
1107	23	0.6292352194276916
1107	24	0.5267637314896945
1107	25	0.7641961544498557
1108	1	0.06739590730054701
1108	2	0.14944670503055946
1108	3	0.12910514503929704
1108	4	0.19069412578505762
1108	5	0.1661275594510272
1108	6	0.0534030308037714
1108	7	0.021674345894112435
1108	8	0.12301713869461395
1108	9	0.2020004464277534
1108	10	0.10729136247547899
1108	11	0.10270150017181834
1108	12	0.2186496099509867
1108	13	0.1256199441714685
1108	14	0.1514097514902133
1108	15	0.3292428897442008
1108	16	0.24872929053699977
1108	17	0.3604889865138219
1108	18	0.23965023332124247
1108	19	0.268067586129483
1108	20	0.3375152073240528
1108	21	0.5041757839654935
1108	22	0.45265140977983975
1108	23	0.33620783965751144
1108	24	0.25592034944968844
1108	25	0.15869537419733604
1109	1	-0.09075632258924671
1109	2	0.24082326286349698
1109	3	-0.12122444606174199
1109	4	0.016991890745408966
1109	5	0.1412048073653338
1109	6	0.15064270533255808
1109	7	0.10984511528334195
1109	8	0.16491297417372408
1109	9	0.04817238311879635
1109	10	0.019802949662619085
1109	11	0.031483214732190845
1109	12	0.04084722728198642
1109	13	0.10771645256574658
1109	14	0.26751079176212755
1109	15	1.4188380469448703E-4
1109	16	-0.017098807104360958
1109	17	-0.1518449304704248
1109	18	-0.15743580368908994
1109	19	-0.0625303127272474
1109	20	-0.01527601731287831
1109	21	0.02281141413681098
1109	22	0.04932116636307882
1109	23	0.25906635581090853
1109	24	0.15411360656508322
1109	25	0.11605710043073003
1110	1	-0.23212321571023153
1110	2	0.05903860483426726
1110	3	-0.12761219030448245
1110	4	0.017900182933352937
1110	5	-0.012049081337934808
1110	6	-0.032777868599204396
1110	7	-0.08435121937407075
1110	8	-0.22970409528464586
1110	9	-0.1118380115056965
1110	10	-0.12519958712073986
1110	11	-0.13418730042045013
1110	12	-0.17068585608537745
1110	13	4.6372707045350126E-4
1110	14	-0.10911367767891278
1110	15	-0.28615824860301586
1110	16	-0.369288484252273
1110	17	-0.34590267013457776
1110	18	-0.2779589997886305
1110	19	-0.25479869203204686
1110	20	-0.2207554693676741
1110	21	-0.15640181178540757
1110	22	-0.266286857297068
1110	23	-0.13108844984673595
1110	24	-0.18830759846888867
1110	25	-0.21725890852844287
1111	1	0.23684343917370754
1111	2	0.4171305886526575
1111	3	0.34095542290788217
1111	4	0.25281083059055476
1111	5	0.19005531361905526
1111	6	0.2205295427357754
1111	7	0.26972599676040687
1111	8	0.28829991084237605
1111	9	0.35692362393674415
1111	10	0.14839139516589483
1111	11	0.2970738440750509
1111	12	0.31344202577314917
1111	13	0.3281452792482966
1111	14	0.33332377568113003
1111	15	0.28272278513485105
1111	16	0.3084759397687762
1111	17	0.32922713623595
1111	18	0.1666181967079723
1111	19	0.34578416017328806
1111	20	0.5096793132410227
1111	21	0.4315507431024628
1111	22	0.5076693895117756
1111	23	0.3991695918641156
1111	24	0.32851106536992347
1111	25	0.21142338988874343
1112	1	-0.12150738541399875
1112	2	0.06917706541427648
1112	3	0.12600040041912663
1112	4	0.1487703508304321
1112	5	0.07594736759207166
1112	6	0.09216372564431907
1112	7	0.04293439256250764
1112	8	0.06809751204177417
1112	9	0.04698359395173668
1112	10	0.10596260955870207
1112	11	-0.01972525637471989
1112	12	0.056230631470299386
1112	13	0.15273298539521818
1112	14	0.029650612262834386
1112	15	0.11883972666409193
1112	16	0.0913919817477904
1112	17	0.058972263672231406
1112	18	0.1357936090018228
1112	19	0.16163123663505985
1112	20	0.25683073752887964
1112	21	0.1228625871804212
1112	22	0.042526960726171126
1112	23	0.015848752283901035
1112	24	0.10742410884818554
1112	25	0.10960929693521848
1113	1	-0.35065563180362247
1113	2	-0.20616186768327793
1113	3	-0.19040838236896462
1113	4	-0.1791326848312238
1113	5	-0.10800488704983735
1113	6	0.04206057209289327
1113	7	0.07044691835089623
1113	8	-0.10816980301864731
1113	9	-0.13970959791387116
1113	10	-0.2571084190295707
1113	11	-0.21828847476552937
1113	12	-0.1752164654940387
1113	13	-0.25045003847746017
1113	14	-0.3585751466525764
1113	15	-0.3584812282430718
1113	16	-0.2706990881019067
1113	17	-0.2946970555143267
1113	18	-0.3500596182307163
1113	19	-0.24757890188542575
1113	20	-0.33528538389759177
1113	21	-0.4061898737685894
1113	22	-0.32150192226823293
1113	23	-0.3703213037137374
1113	24	-0.43723401080993085
1113	25	-0.3699515636601234
1114	1	-0.5604236415716315
1114	2	-1.002450896115164
1114	3	-0.5880448728626
1114	4	-0.5080616923316583
1114	5	-0.3816705071440296
1114	6	-0.5998372126620382
1114	7	-0.4544266208083556
1114	8	-0.6526273296190297
1114	9	-0.5350654325554203
1114	10	-0.6415450141804513
1114	11	-0.664036774085254
1114	12	-0.5632157677790559
1114	13	-0.556755637640201
1114	14	-0.549225765874624
1114	15	-0.6882562151609193
1114	16	-0.6809162518904941
1114	17	-0.8121748158492323
1114	18	-0.7765509840077911
1114	19	-0.7661250382887062
1114	20	-0.6068098911363791
1114	21	-0.608248132969706
1114	22	-0.7525938462172945
1114	23	-0.6706396361749256
1114	24	-0.7386643265259636
1114	25	-0.9010522090464832
1115	1	-0.546692010639326
1115	2	-0.8240615808612368
1115	3	-0.4945017220440917
1115	4	-0.4044177052258533
1115	5	-0.2270735081420696
1115	6	-0.22690355353228142
1115	7	-0.3547948625401247
1115	8	-0.4174043196730608
1115	9	-0.4057319959767369
1115	10	-0.4301774148641613
1115	11	-0.8106437305271896
1115	12	-0.5021690894112467
1115	13	-0.47465551259861627
1115	14	-0.4560009548709658
1115	15	-0.5528384730219003
1115	16	-0.6757927016272234
1115	17	-0.6857680980187624
1115	18	-0.6604673667497636
1115	19	-0.6223630646965717
1115	20	-0.6887149971922923
1115	21	-0.5042685984914925
1115	22	-0.5375538673252365
1115	23	-0.6706009191704183
1115	24	-0.5403414898027421
1115	25	-0.5974936861432241
1116	1	-0.7053930690313344
1116	2	-1.1493691575831733
1116	3	-0.7366287422080013
1116	4	-0.5450677009607929
1116	5	-0.45385942162032933
1116	6	-0.5527846173048232
1116	7	-0.5361545649042796
1116	8	-0.5625713461361386
1116	9	-0.6805556519894935
1116	10	-0.6974722488875642
1116	11	-0.9574583839743482
1116	12	-0.7756088962366618
1116	13	-0.7391360606265138
1116	14	-1.0425686283114288
1116	15	-0.9261555772403796
1116	16	-0.9257923735453844
1116	17	-0.9962135675366781
1116	18	-1.0374386464657732
1116	19	-1.1326864877712168
1116	20	-1.0005220063326519
1116	21	-1.1319173672003586
1116	22	-1.189319275688696
1116	23	-1.0395886554713627
1116	24	-1.4222451518522021
1116	25	-1.294731237077933
1201	1	0.6072782217824049
1201	2	0.8081450814062805
1201	3	0.69651097846344
1201	4	0.5039219417016625
1201	5	0.6361422701407946
1201	6	0.663911185247855
1201	7	0.7628660933787685
1201	8	0.702309572678125
1201	9	0.7212714500236999
1201	10	0.7913865642480218
1201	11	0.8797791580847161
1201	12	0.680301929154794
1201	13	0.7440873082338015
1201	14	1.0149662877584262
1201	15	1.0036889687097315
1201	16	0.91852424894752
1201	17	1.0492271362359489
1201	18	1.1489370372876806
1201	19	1.1289725659703933
1201	20	1.1145102311154176
1201	21	1.063338182715988
1201	22	0.9912442687388321
1201	23	0.9697009928302975
1201	24	1.185322659572826
1201	25	1.1787663850578198
1202	1	0.23160554731413896
1202	2	0.455307747617379
1202	3	0.3093162326798158
1202	4	-0.001542974825007093
1202	5	0.15333711083439994
1202	6	0.11516352240605698
1202	7	0.15561307286871795
1202	8	0.2023855006604336
1202	9	0.26674851502754393
1202	10	0.3936463708214859
1202	11	0.24444891881665853
1202	12	0.24941537505135614
1202	13	0.3675490547672169
1202	14	0.4412250313401387
1202	15	0.3412467451587702
1202	16	0.5546346482338009
1202	17	0.6362434512611514
1202	18	0.5746595394943412
1202	19	0.48640259358919735
1202	20	0.3951580866244697
1202	21	0.5840389979926996
1202	22	0.3933873360394955
1202	23	0.47913884103127913
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2210	18	0.07901523193376647
2210	19	0.24274450249773594
2210	20	0.23706526876940615
2210	21	0.2762729865501612
2210	22	0.08752726373811015
2210	23	0.0016147664648613613
2210	24	0.29701110357577676
2210	25	0.18692885332573894
2211	1	-0.07005934964076133
2211	2	-0.4659306744494742
2211	3	-0.1302066461881175
2211	4	-0.16053234116560589
2211	5	-0.18249775710406624
2211	6	-0.05275989609572207
2211	7	-0.02278242265911956
2211	8	-0.1251522403666172
2211	9	-0.1472947890499725
2211	10	0.06694817421762167
2211	11	-0.04970709965673592
2211	12	-0.13778815548086953
2211	13	-0.24065556836967517
2211	14	-0.0698737117370456
2211	15	-0.06835603576801219
2211	16	-0.01051297308550403
2211	17	-0.012331118579197664
2211	18	0.19731937353470386
2211	19	0.169597684220669
2211	20	0.19003652366974677
2211	21	0.19709280236003934
2211	22	0.0878752395893514
2211	23	0.04013148689571011
2211	24	0.1538775466201585
2211	25	0.15573186228439795
2212	1	-0.2470037281517179
2212	2	-0.8883352498566595
2212	3	-0.47304796500854707
2212	4	-0.1310363367348728
2212	5	-0.1204923995373548
2212	6	-0.20886627883395903
2212	7	-0.1350697871658113
2212	8	-0.09500518985403517
2212	9	0.04587148766721327
2212	10	-0.007546869468022521
2212	11	-0.17369973017678575
2212	12	-0.09667278695035719
2212	13	-0.003663491690915066
2212	14	-0.10271989079731725
2212	15	-0.026413296175142875
2212	16	0.02067620327338915
2212	17	-1.2324592932765697
2212	18	-0.5055707737418658
2212	19	-0.21162596593249106
2212	20	-0.11079875941096162
2212	21	-0.12865629623500752
2212	22	-0.12460992537623383
2212	23	-0.11106944446187583
2212	24	-0.2565369301128513
2212	25	-0.038838232546794416
2213	1	-0.39127002121938403
2213	2	-0.882121942615953
2213	3	-0.48218335017133285
2213	4	-0.39636427363815585
2213	5	-0.34663765838809013
2213	6	-0.25620660973593345
2213	7	-0.20402474773035806
2213	8	-0.4625413637258191
2213	9	-0.3175239639131675
2213	10	-0.3538271873236809
2213	11	-0.4707998902906425
2213	12	-0.41657286404221594
2213	13	-0.2712219035986365
2213	14	-0.47495496166252593
2213	15	-0.5605887098026088
2213	16	-0.4112768892961398
2213	17	-1.5003038345800093
2213	18	-1.2434517854242486
2213	19	-0.978797436014915
2213	20	-0.6488376611484981
2213	21	-0.48192602878556556
2213	22	-0.5752222126323545
2213	23	-0.5660477994402342
2213	24	-0.4362402747134553
2213	25	-0.28616892696653196
2214	1	-0.7669072511035075
2214	2	-2.08857717558546
2214	3	-1.0245638496319707
2214	4	-0.7252836361472887
2214	5	-0.638930439059223
2214	6	-0.7944607013462459
2214	7	-0.7083795144128757
2214	8	-0.757633896174525
2214	9	-0.8566617679566377
2214	10	-0.923644094286665
2214	11	-0.8426463635603341
2214	12	-0.8742228124258702
2214	13	-0.734414084592224
2214	14	-0.7932712248710914
2214	15	-0.8264418873976354
2214	16	-0.8260997698361976
2214	17	-0.794427835600743
2214	18	-0.7237672540890685
2214	19	-0.7578686027763126
2214	20	-0.8727453084870618
2214	21	-0.6750052337778527
2214	22	-0.7999954486872412
2214	23	-0.7146506783564064
2214	24	-0.6619450698139673
2214	25	-0.7439732894176672
2215	1	-0.8962632405691267
2215	2	-1.988084623290245
2215	3	-1.2478789819448295
2215	4	-1.1425347798122658
2215	5	-1.0513954146508908
2215	6	-0.9411667269238819
2215	7	-1.204516130296362
2215	8	-1.3026303132686294
2215	9	-1.4164206062038218
2215	10	-1.4938991137150026
2215	11	-1.4329083578064612
2215	12	-1.42859349781844
2215	13	-1.565796095928782
2215	14	-1.4604323537729442
2215	15	-1.5424154227035842
2215	16	-1.4213834914178847
2215	17	-1.5261024921829218
2215	18	-1.603603734932726
2215	19	-1.5776926477886448
2215	20	-1.4855842078806205
2215	21	-1.4494583012475388
2215	22	-1.400314337922751
2215	23	-1.5422382756307087
2215	24	-1.5614355402746227
2215	25	-1.3955026207901327
2216	1	-0.8084379608467209
2216	2	-1.7794640114631015
2216	3	-0.9711960189905895
2216	4	-0.8362450408472597
2216	5	-0.730021633028997
2216	6	-0.7910485444931226
2216	7	-0.6568941387474041
2216	8	-0.6958987885549917
2216	9	-0.7565740438307813
2216	10	-0.701695425167081
2216	11	-0.9031488245436563
2216	12	-1.026525288925022
2216	13	-0.9364388343331314
2216	14	-0.9894742223441355
2216	15	-1.0794739395255277
2216	16	-1.0597158140019474
2216	17	-1.1514265698006199
2216	18	-1.0784936483092196
2216	19	-0.9943418144275071
2216	20	-1.181601201552482
2216	21	-1.0987498935799778
2216	22	-1.0924702039647798
2216	23	-1.2579037117346772
2216	24	-1.279909485513599
2216	25	-1.1443520348499447
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8754.0
3	0.0
4	110.0
5	1227.0
6	2045.0
7	1433.0
8	1121.0
9	737.0
10	822.0
11	717.0
12	663.0
13	602.0
14	556.0
15	585.0
16	615.0
17	726.0
18	748.0
19	1194.0
20	1596.0
21	1896.0
22	2317.0
23	2765.0
24	3329.0
25	3794.0
26	4279.0
27	4940.0
28	5602.0
29	6859.0
30	8970.0
31	11617.0
32	15510.0
33	20986.0
34	29456.0
35	42032.0
36	64862.0
37	119856.0
38	337919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.163179061692226	14.40128469703266	14.33268300241585	23.102853238859268
2	9.297443382350872	22.86894627756358	38.2900668472132	29.54354349287235
3	22.500843597098026	30.17813958719982	26.62631460547776	20.694702210224396
4	14.40995030760284	14.874628433513712	31.073871148980032	39.64155010990342
5	17.18141368216558	32.36315653983094	29.484840342848944	20.970589435154537
6	38.54865010502857	28.457465892095634	12.99427794794016	19.99960605493564
7	25.08510482663936	32.12756439832998	22.658421579770653	20.128909195260015
8	26.236784774485	33.76923477216438	24.615585843939034	15.378394609411588
9	28.53884200356363	21.464040963445385	14.69975656507841	35.297360467912576
10	13.772402882756197	25.391597820355074	29.996835999296888	30.839163297591842
11	30.50768560057378	26.185466972309335	16.759953309800725	26.546894117316157
12	27.049349779978538	23.51044275715228	25.1131046722742	24.327102790594978
13	33.14973374074913	18.89589945285728	26.80605611482263	21.148310691570966
14	32.906926382576614	18.885276515321962	22.578803491877782	25.628993610223645
15	36.369963457517784	16.82388323277941	26.39255821277876	20.41359509692405
16	27.898795756676765	20.210619556124207	32.73376591421963	19.156818772979396
17	25.38172768685676	28.64166807266183	28.570243518362297	17.406360722119114
18	28.162926719532084	16.590883527360667	38.7412125302289	16.504977222878352
19	24.216579494966535	35.202322704009894	18.89446600303695	21.686631797986614
20	18.927788088408974	28.346127889320062	30.277262246217873	22.44882177605309
21	20.060320160853763	33.96840573393044	21.121055110692417	24.85021899452338
22	19.069708580869687	31.081941856786305	17.086792983442894	32.761556578901114
23	23.233249207364654	24.506527378433287	18.680745463876217	33.579477950325845
24	17.85983940429708	30.059601224362968	19.49307323822071	32.58748613311924
25	27.24484481666549	27.410373706805547	18.81708776127806	26.527693715250898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	356.0
1	356.0
2	463.0
3	570.0
4	570.0
5	570.0
6	1318.5
7	2067.0
8	2067.0
9	2067.0
10	1785.5
11	1504.0
12	1504.0
13	1504.0
14	1388.0
15	1272.0
16	1272.0
17	1272.0
18	1982.0
19	2692.0
20	2692.0
21	2692.0
22	3932.5
23	5173.0
24	5173.0
25	5173.0
26	7682.5
27	10192.0
28	10192.0
29	10192.0
30	11926.5
31	13661.0
32	13661.0
33	13661.0
34	16161.5
35	18662.0
36	18662.0
37	18662.0
38	21023.0
39	23384.0
40	23384.0
41	23384.0
42	31035.5
43	38687.0
44	38687.0
45	38687.0
46	96961.5
47	155236.0
48	155236.0
49	155236.0
50	227508.0
51	299780.0
52	299780.0
53	299780.0
54	184254.0
55	68728.0
56	68728.0
57	68728.0
58	46487.5
59	24247.0
60	24247.0
61	24247.0
62	20701.0
63	17155.0
64	17155.0
65	17155.0
66	14308.0
67	11461.0
68	11461.0
69	11461.0
70	8986.0
71	6511.0
72	6511.0
73	6511.0
74	5382.0
75	4253.0
76	4253.0
77	4253.0
78	3704.5
79	3156.0
80	3156.0
81	3156.0
82	2346.5
83	1537.0
84	1537.0
85	1537.0
86	1109.5
87	682.0
88	682.0
89	682.0
90	465.0
91	248.0
92	248.0
93	248.0
94	134.5
95	21.0
96	21.0
97	21.0
98	13.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1891063494741578
2	0.00927956807828581
3	0.0
4	0.15058208199763792
5	0.052443619593948596
6	0.06762836735841629
7	0.04949102975085766
8	0.03135369214329903
9	0.023480119228389856
10	0.01616894437883134
11	0.023901917777402845
12	0.024323716326415838
13	0.012794555986727407
14	0.014622349699117035
15	0.0018277937123896294
16	7.029975816883191E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.004920983071818233
22	0.009138968561948147
23	7.029975816883191E-4
24	0.0018277937123896294
25	0.1110736179067544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	711240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.262184377873382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.10634206293754	20.495459739280342
2	6.058638298969866	2.8187432237896437
3	1.6197276118628718	1.1303520704725998
4	0.807436096005985	0.7513090935456599
5	0.5346153686303998	0.6218160638162553
6	0.38362046540652106	0.5354309998447254
7	0.28314988285311643	0.46106793470517105
8	0.19958473145904382	0.37142214577668986
9	0.1830780893417783	0.3832916642835501
>10	1.3960434943015119	6.5649340177488265
>50	0.20903476613291658	3.4280662436343694
>100	0.15208036608510742	6.913356189880184
>500	0.019994629804018105	3.3205251473473445
>1k	0.03514207662524395	16.517213018060524
>5k	0.006058978728490335	9.683631743643588
>10k+	0.005453080855641302	26.00338070417053
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	41619	5.85161127045723	No Hit
CTTCCGTACTCTGCGTTGATACCAC	27841	3.914431134356898	No Hit
GAGTACGGAAGCAGTGGTATCAACG	23895	3.359625442888477	No Hit
TCCGTACTCTGCGTTGATACCACTG	22753	3.1990607952308645	No Hit
GCTTCCGTACTCTGCGTTGATACCA	17317	2.434761824419324	No Hit
CCCATGTACTCTGCGTTGATACCAC	15411	2.166779146279737	No Hit
GTACATGGGAAGCAGTGGTATCAAC	13856	1.9481468983746697	No Hit
GCGTTGATACCACTGCTTCCGTACT	11412	1.6045216804454192	No Hit
GTATCAACGCAGAGTACGGAAGCAG	10389	1.4606883752319892	No Hit
TCCATGTACTCTGCGTTGATACCAC	9466	1.3309150216523256	No Hit
GTACATGGAAGCAGTGGTATCAACG	9188	1.2918283561104549	No Hit
TATCAACGCAGAGTACGGAAGCAGT	8479	1.1921432990270513	No Hit
ACTCTGCGTTGATACCACTGCTTCC	8147	1.145464259602947	No Hit
CAGTGGTATCAACGCAGAGTACGGA	6327	0.8895731398683989	No Hit
CATGTACTCTGCGTTGATACCACTG	5936	0.8345987289803724	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	5640	0.7929812721444239	No Hit
ATACCACTGCTTCCGTACTCTGCGT	5237	0.7363196670603452	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5154	0.7246499072043192	No Hit
GGTATCAACGCAGAGTACGGAAGCA	5131	0.7214161183285529	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4336	0.6096395028401101	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	4103	0.5768798155334346	No Hit
GCGTTGATACCACTGCTTCCCATGT	4069	0.572099431977954	No Hit
GTGGTATCAACGCAGAGTACGGAAG	4044	0.5685844440695125	No Hit
GTATCAACGCAGAGTACATGGGAAG	3808	0.5354029582138238	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3279	0.4610258140711996	No Hit
GTACTCTGCGTTGATACCACTGCTT	3252	0.45722962713008264	No Hit
TATCAACGCAGAGTACATGGGAAGC	3204	0.45048085034587476	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	3047	0.42840672628086157	No Hit
GTACGGGAAGCAGTGGTATCAACGC	2847	0.4002868230133288	No Hit
GATACCACTGCTTCCGTACTCTGCG	2837	0.3988808278499522	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2722	0.38271188347112084	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	2721	0.3825712839547832	No Hit
GTTGATACCACTGCTTCCGTACTCT	2664	0.3745571115235363	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2644	0.3717451211967831	No Hit
CAGTGGTATCAACGCAGAGTACATG	2560	0.35993476182441936	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	2516	0.3537483831055621	No Hit
CCCGTACTCTGCGTTGATACCACTG	2408	0.3385636353410944	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2355	0.33111186097519824	No Hit
TTCCCGTACTCTGCGTTGATACCAC	2289	0.32183229289691245	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	2223	0.3125527248186266	No Hit
AAAAAGTACTCTGCGTTGATACCAC	2178	0.3062257465834317	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1990	0.279793037511951	No Hit
GCGTTGATACCACTGCTTCCATGTA	1960	0.27557505202182103	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1919	0.2698104718519768	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1766	0.24829874585231426	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1741	0.24478375794387267	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1736	0.24408076036218437	No Hit
CCATGTACTCTGCGTTGATACCACT	1717	0.24140936955176873	No Hit
GGTATCAACGCAGAGTACATGGGAA	1698	0.23873797874135314	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1678	0.23592598841459983	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1658	0.2331139980878466	No Hit
GTATCAACGCAGAGTACATGGAAGC	1646	0.2314268038917946	No Hit
ATACCACTGCTTCCCATGTACTCTG	1544	0.21708565322535292	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1524	0.21427366289859962	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1489	0.20935267982678138	No Hit
TGATACCACTGCTTCCGTACTCTGC	1465	0.20597829143467744	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1424	0.20021371126483328	No Hit
AAAGTACTCTGCGTTGATACCACTG	1386	0.19487092964400202	No Hit
TATCAACGCAGAGTACATGGAAGCA	1369	0.19248073786626174	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1362	0.1914965412518981	No Hit
TACCACTGCTTCCGTACTCTGCGTT	1358	0.19093414318654744	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1348	0.1895281480231708	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1288	0.18109217704291097	No Hit
CTGCGTTGATACCACTGCTTCCGTA	1282	0.180248579944885	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	1269	0.17842078623249535	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1266	0.17799898768348238	No Hit
TACCACTGCTTCCCATGTACTCTGC	1252	0.17603059445475508	No Hit
GTGGTATCAACGCAGAGTACATGGG	1203	0.16914121815420957	No Hit
GCGTTGATACCACTGCTTCCCGTAC	1180	0.16590742927844326	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1146	0.16112704572296271	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1132	0.15915865249423541	No Hit
CCCCATGTACTCTGCGTTGATACCA	1107	0.15564366458579385	No Hit
TCCCATGTACTCTGCGTTGATACCA	1057	0.14861368876891062	No Hit
TATCAACGCAGAGTACGGGAAGCAG	1055	0.1483324897362353	No Hit
TACGGAAGCAGTGGTATCAACGCAG	1048	0.14734829312187167	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	1013	0.14242731005005344	No Hit
GCCCATGTACTCTGCGTTGATACCA	1007	0.14158371295202743	No Hit
ATACCACTGCTTCCATGTACTCTGC	955	0.13427253810246895	No Hit
CTCTGCGTTGATACCACTGCTTCCG	948	0.13328834148810528	No Hit
GATACCACTGCTTCCCATGTACTCT	933	0.13117934874304032	No Hit
GTTGATACCACTGCTTCCCATGTAC	929	0.13061695067768966	No Hit
CCACTGCTTCCCATGTACTCTGCGT	923	0.12977335357966369	No Hit
GCTTCCCGTACTCTGCGTTGATACC	918	0.12907035599797537	No Hit
ACATGGGAAGCAGTGGTATCAACGC	916	0.12878915696530002	No Hit
CTTCCCATGTACTCTGCGTTGATAC	907	0.12752376131826107	No Hit
GTACGGGAGCAGTGGTATCAACGCA	846	0.11894719082166356	No Hit
ATCAACGCAGAGTACATGGGAAGCA	823	0.1157134019458973	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	786	0.11051121984140375	No Hit
GGTATCAACGCAGAGTACTTTTTTT	761	0.10699623193296216	No Hit
GGTATCAACGCAGAGTACATGGAAG	734	0.10320004499184522	No Hit
AACGCAGAGTACGGAAGCAGTGGTA	723	0.10165345031213092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.405995163376638E-4	0.0	0.0	0.0	0.0
6	1.405995163376638E-4	0.0	0.0	0.0	0.0
7	1.405995163376638E-4	0.0	0.0	0.0	0.0
8	1.405995163376638E-4	0.0	0.0	0.0	0.0
9	1.405995163376638E-4	0.0	0.0	0.0	0.0
10	1.405995163376638E-4	0.0	0.0	4.217985490129914E-4	0.0
11	1.405995163376638E-4	0.0	0.0	9.841966143636467E-4	0.0
12	1.405995163376638E-4	0.0	0.0	0.001405995163376638	0.0
13	1.405995163376638E-4	0.0	0.0	0.0016871941960519656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGTT	25	0.006042871	18.992403	7
TTATCAA	65	9.824362E-9	17.56357	2
TAGAGTG	40	2.7594846E-4	16.624199	5
AAGCTCG	40	2.7669003E-4	16.618353	7
GCTCGTA	35	0.002175465	16.278057	9
ATTGACG	35	0.0021764874	16.276913	11
GTTATCA	75	5.567017E-8	15.25724	1
CGGTCCA	75	5.8687874E-8	15.191786	10
GACATGG	85	1.679291E-8	14.550212	2
AGGAATT	40	0.0052893884	14.244303	7
GTGGGTA	40	0.0052918573	14.2433	9
AGTGTTC	40	0.0052918573	14.2433	8
TGACGGA	40	0.0053017433	14.239295	13
CCGACCA	95	4.9167284E-9	13.993418	9
CGACCAT	95	4.9203663E-9	13.992434	10
AGGCCCG	75	9.710475E-7	13.925804	10
ACGATGC	105	1.3624231E-9	13.57364	19
GGTCCAA	85	2.7098213E-7	13.404517	11
AAGAATT	85	2.7191345E-7	13.400747	16
GCTTACG	50	0.0014563006	13.350083	1
>>END_MODULE