FastQCFastQC Report
Thu 2 Feb 2017
SRR4050245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537669
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66540.2622091375983235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN55620.21917752078777808No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN41780.16463928116708682No Hit
TATCAACGCAGAGTACTTTTTTTTT40010.1576643762444984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9850.015.3377161
GTATCAA36750.014.0650521
TAGATCG701.0900374E-412.2159445
GTCGGCC803.774613E-410.686830512
ATTAGCC1201.5219866E-610.2930643
CGGTCCA1951.4551915E-1110.22992510
CCGACCA2151.8189894E-1210.1617519
GCCGTTA750.002649488910.132915
CGTCGTA1803.947207E-1010.02695110
CCGCGCA951.6464427E-49.9989789
AAAACCG1054.100841E-59.9537325
CGACCAT2400.09.89501710
TTAATAC3200.09.7982053
ATCGCCA1752.575689E-99.77120416
GTATTAG4100.09.7334161
TATCAAC53500.09.5904672
TCGCACG1201.7012331E-59.499969518
AACGTCT1401.059454E-69.4997826
ATACCGT2106.730261E-119.4997816
GATAACG900.0011150629.49902811
Produced by FastQC (version 0.11.5)