##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050245_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2537669
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.71043032010873	31.0	30.0	34.0	25.0	34.0
2	29.825217552013285	31.0	30.0	34.0	25.0	34.0
3	29.943954471603664	31.0	31.0	34.0	26.0	34.0
4	33.31382776871215	37.0	35.0	37.0	27.0	37.0
5	33.3922800806567	37.0	35.0	37.0	28.0	37.0
6	33.4478649500782	37.0	35.0	37.0	28.0	37.0
7	33.32653549379371	37.0	35.0	37.0	27.0	37.0
8	33.274328527479355	37.0	35.0	37.0	27.0	37.0
9	34.72918493310199	39.0	35.0	39.0	27.0	39.0
10	34.63196736847871	39.0	35.0	39.0	27.0	39.0
11	34.651122348895775	39.0	35.0	39.0	27.0	39.0
12	34.541197059190935	39.0	35.0	39.0	27.0	39.0
13	34.53090848333648	39.0	35.0	39.0	27.0	39.0
14	35.400886404018806	40.0	36.0	41.0	25.0	41.0
15	35.51971868671604	40.0	36.0	41.0	26.0	41.0
16	35.53182192003764	40.0	36.0	41.0	26.0	41.0
17	35.51216766252809	40.0	36.0	41.0	26.0	41.0
18	35.351316897515005	40.0	36.0	41.0	25.0	41.0
19	35.478777965132565	40.0	36.0	41.0	25.0	41.0
20	35.44217705303568	40.0	36.0	41.0	25.0	41.0
21	35.40906950433646	40.0	36.0	41.0	25.0	41.0
22	35.33234082143889	40.0	36.0	41.0	25.0	41.0
23	35.2289250489327	40.0	36.0	41.0	25.0	41.0
24	35.173333086387544	40.0	36.0	41.0	24.0	41.0
25	34.8875270967175	39.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9459804960752649
1101	2	0.9824377867100367
1101	3	0.8723843102763915
1101	4	1.0319115508323264
1101	5	0.9676098201871284
1101	6	0.9770715957837055
1101	7	0.9895505315259214
1101	8	1.0038231976672876
1101	9	1.0797232573647335
1101	10	1.2222939558684303
1101	11	1.2041726791858522
1101	12	1.2083357247616604
1101	13	1.1958964052111796
1101	14	1.6175898752171491
1101	15	1.4898597304772494
1101	16	1.4628291384580336
1101	17	1.3406342738891652
1101	18	1.5094624028835355
1101	19	1.4209362942135613
1101	20	1.3560011446276476
1101	21	1.3160410283527213
1101	22	1.3954661427860628
1101	23	1.4623815389606634
1101	24	1.5067007189198947
1101	25	1.482599881382363
1102	1	1.0531288504605172
1102	2	0.9849772756484576
1102	3	0.9432132081892348
1102	4	1.0637464653245843
1102	5	1.0428814607195775
1102	6	1.0337258485407261
1102	7	0.9820881999402076
1102	8	0.9794685513483472
1102	9	1.002046059682364
1102	10	1.1747761425454755
1102	11	1.0292004769458458
1102	12	1.1517137104528459
1102	13	1.24055108086079
1102	14	1.4804500450568767
1102	15	1.3147194616212303
1102	16	1.253063274929076
1102	17	1.2308602243228677
1102	18	1.3707061464121395
1102	19	1.2629939732222866
1102	20	1.2101223245295714
1102	21	1.1747860550979894
1102	22	1.2455444000387672
1102	23	1.203700625639101
1102	24	1.419584293240895
1102	25	1.3384638338666832
1103	1	0.8485623312474431
1103	2	0.8542369950819833
1103	3	0.7603167830081752
1103	4	0.8557092834686557
1103	5	0.8382065971060584
1103	6	0.7293717086313833
1103	7	0.7604278477996615
1103	8	0.8088362874177477
1103	9	0.8715300550596652
1103	10	0.8758389191727076
1103	11	0.8847071342085044
1103	12	1.0038984130732658
1103	13	1.0573731726785667
1103	14	1.4058399028070525
1103	15	1.257238356641885
1103	16	1.1880288657186426
1103	17	1.1430932175903408
1103	18	1.1933886991397458
1103	19	1.1218655283559258
1103	20	1.1054680068989882
1103	21	1.1100355567744487
1103	22	1.1988106347036336
1103	23	1.2501956994589634
1103	24	1.2696081775667025
1103	25	1.3028646559487882
1104	1	0.7062448248467739
1104	2	0.5903240017258291
1104	3	0.6506241871358043
1104	4	0.7748294439671284
1104	5	0.6506247247615349
1104	6	0.609090910864964
1104	7	0.6595305785219523
1104	8	0.7090100976734988
1104	9	0.7365172537235551
1104	10	0.796150458498964
1104	11	0.8346094322548083
1104	12	0.800052047459765
1104	13	0.819888600812142
1104	14	1.1299724000770226
1104	15	1.0291276663791535
1104	16	0.9549496282010992
1104	17	0.8566464027829994
1104	18	1.022705794888239
1104	19	0.9815919628855809
1104	20	0.8875979553724633
1104	21	0.8739445353746476
1104	22	1.0002020917617997
1104	23	0.9101603524005242
1104	24	1.0017648815208986
1104	25	0.9897058058207762
1105	1	0.5539265393187414
1105	2	0.6982078022473424
1105	3	0.6083083461970809
1105	4	0.7079536291040256
1105	5	0.7014762567041686
1105	6	0.6677651285963861
1105	7	0.6279913021203853
1105	8	0.6706830116239573
1105	9	0.7942095614158617
1105	10	0.7555336071299905
1105	11	0.7985150711074809
1105	12	0.8361015911364262
1105	13	0.773042121020751
1105	14	1.0932750402334719
1105	15	0.8859317732891938
1105	16	1.005772639935124
1105	17	0.9529387756643573
1105	18	1.0358210165310027
1105	19	0.7866033709690257
1105	20	0.8379687180843263
1105	21	0.7918918952181926
1105	22	0.7044475285284264
1105	23	0.8493267474461561
1105	24	0.775509992342279
1105	25	0.722174026159756
1106	1	0.4322270484555766
1106	2	0.4278356058948525
1106	3	0.387884874928762
1106	4	0.5602292252543606
1106	5	0.48353732770437574
1106	6	0.4791846845081196
1106	7	0.4450517008271788
1106	8	0.4734782136564917
1106	9	0.5721804795412311
1106	10	0.5702527523741381
1106	11	0.6029414375856561
1106	12	0.5924814549786106
1106	13	0.6767423437137623
1106	14	0.9416811696763787
1106	15	0.7567234673548882
1106	16	0.7434485650744165
1106	17	0.74857843442026
1106	18	0.886665664573691
1106	19	0.7669390226554782
1106	20	0.8169705530105418
1106	21	0.7785589454154334
1106	22	0.8483089544727989
1106	23	0.768696156289522
1106	24	0.7933578105327328
1106	25	0.6744763802570333
1107	1	0.3049539896909508
1107	2	0.23101734508285432
1107	3	0.32285798976018754
1107	4	0.48537396966423074
1107	5	0.41871435707427196
1107	6	0.25740880999016724
1107	7	0.2620114423819828
1107	8	0.18016492954884455
1107	9	0.3884658936907499
1107	10	0.42143664630651045
1107	11	0.38362443362167653
1107	12	0.3864209649009922
1107	13	0.26816549392313505
1107	14	0.5537268423952284
1107	15	0.447747814000806
1107	16	0.390510385445495
1107	17	0.4012660720011567
1107	18	0.5438367065886212
1107	19	0.4555275150999094
1107	20	0.3799947569033577
1107	21	0.3213927652817006
1107	22	0.49394867459395186
1107	23	0.5079383390052215
1107	24	0.4685500100064104
1107	25	0.4737818036337842
1108	1	0.3309377692447697
1108	2	0.36461728561357987
1108	3	0.30298525602182025
1108	4	0.39910396407893955
1108	5	0.2036624771620268
1108	6	0.36515958047893804
1108	7	0.2945881999402076
1108	8	0.2976984965401783
1108	9	0.37277974179496454
1108	10	0.40649637174328745
1108	11	0.39270944555570964
1108	12	0.40149572340750694
1108	13	0.36063080183736673
1108	14	0.7248446638909627
1108	15	0.5306512005350328
1108	16	0.4869633745802915
1108	17	0.38759290992326356
1108	18	0.511556918709104
1108	19	0.4735694590219879
1108	20	0.44361759109658294
1108	21	0.44210045469938564
1108	22	0.43525541149865177
1108	23	0.49845025194701975
1108	24	0.5712098537790098
1108	25	0.5161544168263248
1109	1	0.34189280410619816
1109	2	0.3940765402532449
1109	3	0.35253754046556196
1109	4	0.5138029590974824
1109	5	0.3733549772527738
1109	6	0.42402089479714533
1109	7	0.43044933057460355
1109	8	0.40017426842467785
1109	9	0.36470535314860797
1109	10	0.4927766204533768
1109	11	0.3199156586883376
1109	12	0.45245203415652924
1109	13	0.48930516562592175
1109	14	0.7386533973415439
1109	15	0.5403957920365201
1109	16	0.5174136419117445
1109	17	0.5450665864923607
1109	18	0.6941963781727196
1109	19	0.568263966435886
1109	20	0.612858031013829
1109	21	0.6089057091564669
1109	22	0.5796583895962826
1109	23	0.5499114182182723
1109	24	0.5249605783401776
1109	25	0.46171581286733243
1110	1	0.04824283994781098
1110	2	0.01317802603501761
1110	3	0.043157207991935564
1110	4	0.06983745854129353
1110	5	0.05299219045388526
1110	6	0.09828732061944123
1110	7	0.13527420637822019
1110	8	0.13329149636361137
1110	9	0.1034226351402836
1110	10	0.04248525998880126
1110	11	0.05003112956126188
1110	12	-0.023102472719536138
1110	13	0.011263915923720447
1110	14	0.12500293347682145
1110	15	0.07320098504105488
1110	16	0.005229429042472589
1110	17	-0.19497034250957768
1110	18	0.1007461909763947
1110	19	0.017293040546910277
1110	20	-0.03048202724067295
1110	21	0.03842758520888623
1110	22	0.038782235476993776
1110	23	0.015464232427106595
1110	24	-0.09796489257146135
1110	25	-0.16936841967347505
1111	1	-0.23740127579056391
1111	2	-0.30858413831719034
1111	3	-0.39919949199163796
1111	4	-0.26463522800470685
1111	5	-0.3008211238534386
1111	6	-0.30043900905857157
1111	7	-0.33707688467240615
1111	8	-0.37454096578904483
1111	9	-0.46538498634221526
1111	10	-0.3699951732131552
1111	11	-0.34218017845743987
1111	12	-0.3302981897964443
1111	13	-0.489702192090526
1111	14	-0.2588320226860077
1111	15	-0.3969661017043933
1111	16	-0.37285566706216144
1111	17	-0.3576924575491134
1111	18	-0.29402494575420945
1111	19	-0.46618560592670377
1111	20	-0.5150892730001075
1111	21	-0.4215599807743402
1111	22	-0.4783332302800787
1111	23	-0.5181812012584928
1111	24	-0.6199094908177045
1111	25	-0.641781388030914
1112	1	-0.587253279936931
1112	2	-0.5510374279767376
1112	3	-0.602512131244854
1112	4	-0.5146383863531909
1112	5	-0.6382965404668326
1112	6	-0.6433567501990254
1112	7	-0.5435547336497635
1112	8	-0.6465475212135914
1112	9	-0.6886932387582974
1112	10	-0.7240778396085616
1112	11	-0.6887806628391928
1112	12	-0.5296411117305624
1112	13	-0.6838141265746103
1112	14	-0.595558093894347
1112	15	-0.6420361375374952
1112	16	-0.9655821103021722
1112	17	-0.8670278277631027
1112	18	-0.8300486873864941
1112	19	-0.7790899426005495
1112	20	-0.8179901398469624
1112	21	-0.7193235759942951
1112	22	-0.8240224766698176
1112	23	-0.9314712485182781
1112	24	-0.7191924476940486
1112	25	-0.7984511012332689
1113	1	-0.5923858193112821
1113	2	-0.7011638833974523
1113	3	-0.6194191717317956
1113	4	-0.4898015371921929
1113	5	-0.5565697985509104
1113	6	-0.671547323915533
1113	7	-0.8762358884293562
1113	8	-0.8539125642146459
1113	9	-0.8001820371512167
1113	10	-0.8331490669522381
1113	11	-0.8167893069582064
1113	12	-0.7884129927056875
1113	13	-0.8765506398705298
1113	14	-0.7809052969141987
1113	15	-0.8171245802959604
1113	16	-0.8374964663123095
1113	17	-0.8745189299407556
1113	18	-0.7624210073572613
1113	19	-0.847881348772404
1113	20	-0.9301610671374547
1113	21	-0.8615161532067432
1113	22	-0.9027603063529526
1113	23	-1.285989898658066
1113	24	-1.2094355788022355
1113	25	-1.2437385163700938
1114	1	-2.489521923121515
1114	2	-2.6800115661601964
1114	3	-2.2912937942658296
1114	4	-5.511498844029372
1114	5	-3.0648057971253273
1114	6	-2.4700648479722425
1114	7	-2.3693528364441683
1114	8	-2.5909774214514236
1114	9	-3.1072952127457363
1114	10	-2.9543071114932893
1114	11	-2.6588763581757746
1114	12	-2.8050694578952147
1114	13	-2.82146060041185
1114	14	-2.7524331054170474
1114	15	-3.3589042300184033
1114	16	-3.34711027503176
1114	17	-3.1148538604951597
1114	18	-3.033943034269072
1114	19	-3.1031459759941455
1114	20	-3.406103052535812
1114	21	-3.229600467804559
1114	22	-3.3366626875812955
1114	23	-3.3960338251781153
1114	24	-3.406144143455755
1114	25	-3.372312035000256
1115	1	-2.6457591940603464
1115	2	-1.987090513796634
1115	3	-1.5513920566311334
1115	4	-1.620176172255345
1115	5	-1.4211771748050879
1115	6	-1.5381245695135775
1115	7	-1.5808988983386136
1115	8	-1.6148323461443788
1115	9	-1.8372636927069337
1115	10	-1.7750339799384847
1115	11	-1.774552285602887
1115	12	-2.181499482500719
1115	13	-2.237131105214175
1115	14	-2.4701036228813393
1115	15	-2.1895099096811137
1115	16	-2.113592288020186
1115	17	-2.106427431888143
1115	18	-2.070459264845688
1115	19	-2.0029526057447598
1115	20	-2.0530361728544975
1115	21	-1.9077919095813982
1115	22	-2.089317833142985
1115	23	-2.0907007975670453
1115	24	-2.4744591963043305
1115	25	-2.545469767043869
1116	1	-1.2477515529553038
1116	2	-1.1591207084983353
1116	3	-1.3049355449582372
1116	4	-1.1333341700295918
1116	5	-1.3966774212198771
1116	6	-1.4136076265872006
1116	7	-1.6280838966010656
1116	8	-1.5001291177447627
1116	9	-1.8609403733950884
1116	10	-2.2772417640270106
1116	11	-2.001682598909767
1116	12	-1.9789255359191316
1116	13	-1.8733009836602434
1116	14	-2.514787373023033
1116	15	-2.503318342368793
1116	16	-2.3569181957464167
1116	17	-2.101134488414374
1116	18	-2.0192826550844245
1116	19	-1.9463570529700789
1116	20	-2.148854682681119
1116	21	-2.248979535155698
1116	22	-2.0836039497909624
1116	23	-2.4267467689772815
1116	24	-2.3978045553298415
1116	25	-2.3049992925936564
1201	1	1.0002069898557195
1201	2	1.0132685442333802
1201	3	1.0137077160609458
1201	4	1.098090400314888
1201	5	1.0034309662244354
1201	6	0.9903509509773158
1201	7	1.0648490124087147
1201	8	1.0754901304752096
1201	9	1.3487019821715833
1201	10	1.2269491344065386
1201	11	1.1834970238783455
1201	12	1.2476799356453796
1201	13	1.2569803830703634
1201	14	1.639875738444438
1201	15	1.5097466423117467
1201	16	1.5086810630113092
1201	17	1.4911869143357421
1201	18	1.5684847993900561
1201	19	1.4766951535972126
1201	20	1.5200922679794786
1201	21	1.3556598074075836
1201	22	1.51905646083393
1201	23	1.5104788103354565
1201	24	1.5680909458930472
1201	25	1.5186841552503552
1202	1	0.8692534142786457
1202	2	0.8485172554023883
1202	3	0.8216393162688362
1202	4	0.9815832182988444
1202	5	0.8438752354271983
1202	6	0.7770519854186162
1202	7	0.7540331035781449
1202	8	0.7543313075564981
1202	9	0.9453836348415336
1202	10	0.9193936831965672
1202	11	0.9133874894163796
1202	12	0.976881373216159
1202	13	0.9149134979722078
1202	14	1.3227575983118953
1202	15	1.2192105921223586
1202	16	1.0907302987693157
1202	17	1.030697548276187
1202	18	1.2581932744498303
1202	19	1.203838579651908
1202	20	1.1911311934050985
1202	21	1.1735692962393998
1202	22	1.2110659295644908
1202	23	1.2716581194898566
1202	24	1.1984733556259641
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2211	9	-0.0015566041729542235
2211	10	0.0023862774140255283
2211	11	-0.11084575551679166
2211	12	-0.19094822513776677
2211	13	-0.22407827457871576
2211	14	0.09013733160693249
2211	15	-0.1803459998973196
2211	16	-0.1733691465531635
2211	17	-0.19862625488259766
2211	18	-0.0504337770534562
2211	19	-0.041463073754734125
2211	20	-0.12715563449652478
2211	21	-0.2348607157357847
2211	22	-0.21941480848353478
2211	23	-0.19584876959489605
2211	24	-0.13932338395703425
2211	25	-0.03936166006444353
2212	1	-0.4242522042949872
2212	2	-0.4990269380880292
2212	3	-0.5115407737812596
2212	4	-0.43839999190672785
2212	5	-0.46893572100593417
2212	6	-0.46544488014833973
2212	7	-0.43601476918142623
2212	8	-0.4731266509907073
2212	9	-0.6221341074408429
2212	10	-0.6557323897989846
2212	11	-0.6803020861133788
2212	12	-0.5360731130736056
2212	13	-0.49441244483482194
2212	14	-0.6084065201081685
2212	15	-0.6995635110240883
2212	16	-0.6473552147536665
2212	17	-0.7919574523960833
2212	18	-0.5527788276007044
2212	19	-0.7282613175444084
2212	20	-0.7057534363130955
2212	21	-0.7015138270264529
2212	22	-0.8013048409013876
2212	23	-0.8672509677783253
2212	24	-0.6662590518240634
2212	25	-0.6762071632933981
2213	1	-0.8257319019577594
2213	2	-0.6232163362208212
2213	3	-0.6553130986080937
2213	4	-0.5834311751632484
2213	5	-0.606010487780523
2213	6	-0.696948769365278
2213	7	-0.72728913343812
2213	8	-0.5986120880975037
2213	9	-0.6564942104727578
2213	10	-0.7720863732213061
2213	11	-0.8120446348242965
2213	12	-0.8795364213764358
2213	13	-0.9437336028965788
2213	14	-0.7195961479243209
2213	15	-0.9654088029991641
2213	16	-0.9173442673934034
2213	17	-1.0193087808195287
2213	18	-1.0197455639937232
2213	19	-0.9500648247477557
2213	20	-0.919380963820096
2213	21	-0.8849558126171218
2213	22	-1.1203040110492353
2213	23	-1.0611963471386758
2213	24	-1.0185774897659954
2213	25	-1.0137444648394904
2214	1	-1.2985001579355426
2214	2	-1.2422618991224184
2214	3	-1.2253773806213175
2214	4	-1.0478792414862923
2214	5	-1.1065029921576084
2214	6	-1.3103727246729733
2214	7	-1.3348966273952456
2214	8	-1.337437915579379
2214	9	-1.4596458877931795
2214	10	-1.4877086032951539
2214	11	-1.5147740778625547
2214	12	-1.4412601297086383
2214	13	-1.5479207449158139
2214	14	-1.5679004161202386
2214	15	-1.8431835511797203
2214	16	-1.750649179987569
2214	17	-1.7031569167551197
2214	18	-1.7446019771590855
2214	19	-1.9002699362724869
2214	20	-1.859086109975756
2214	21	-1.9087994246139743
2214	22	-1.967368141387773
2214	23	-2.008885675289939
2214	24	-2.01208725963253
2214	25	-1.9570849815329154
2215	1	-1.6772107444501216
2215	2	-1.79764255613647
2215	3	-1.6211770658039413
2215	4	-1.830191945214608
2215	5	-1.7230906572026221
2215	6	-1.653104724120034
2215	7	-1.662568450348374
2215	8	-1.8150539989123402
2215	9	-2.124142871164935
2215	10	-1.693519124259936
2215	11	-1.9017559780930995
2215	12	-2.096270229727814
2215	13	-2.0513632238573862
2215	14	-2.745275704294073
2215	15	-2.265950287558077
2215	16	-2.0528513409011637
2215	17	-2.1134386470913
2215	18	-1.9939678832400602
2215	19	-2.1661642004426156
2215	20	-2.407039797040156
2215	21	-2.282370595190592
2215	22	-2.49425648823982
2215	23	-2.2501751333670583
2215	24	-2.2603336911820477
2215	25	-2.3003302314024836
2216	1	-1.3761908009680894
2216	2	-1.1750646879743023
2216	3	-1.2326303760975748
2216	4	-1.035723033216705
2216	5	-1.7133868462945294
2216	6	-1.3770849365926665
2216	7	-1.4945641085706143
2216	8	-1.8951579270983387
2216	9	-1.8222180787405904
2216	10	-1.7685863407187696
2216	11	-1.67814724962264
2216	12	-1.9962321767905635
2216	13	-1.7701130289140607
2216	14	-1.7273246990103317
2216	15	-1.6877318512218835
2216	16	-1.7003551305994264
2216	17	-1.7357183299418963
2216	18	-2.111766988684124
2216	19	-1.8261736824859938
2216	20	-1.9150951671829475
2216	21	-2.0448747279891037
2216	22	-2.2606111416149943
2216	23	-1.9999561730232074
2216	24	-2.2187533714126104
2216	25	-2.22332792951169
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108273.0
3	8.0
4	816.0
5	4615.0
6	6050.0
7	5691.0
8	5235.0
9	4676.0
10	4255.0
11	4153.0
12	3897.0
13	3893.0
14	3681.0
15	3805.0
16	3837.0
17	4158.0
18	4658.0
19	5521.0
20	7267.0
21	9347.0
22	11729.0
23	13917.0
24	16222.0
25	18379.0
26	20831.0
27	22931.0
28	26104.0
29	31027.0
30	38657.0
31	48745.0
32	62432.0
33	81909.0
34	110441.0
35	152993.0
36	223167.0
37	386038.0
38	1078311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00115482790339	17.916064864676997	12.300891911030858	25.78188839638875
2	16.03650989976314	20.555317843684108	38.68729644406393	24.72087581248882
3	18.503116442077165	24.962744657952737	29.096600583871908	27.43753831609819
4	12.425201528071957	16.61796099236638	38.2516480548729	32.705189424688754
5	14.689591356002857	36.09817546200462	34.31675115548256	14.89548202650996
6	31.659772172265566	37.31932695771113	17.135793771312237	13.885107098711073
7	27.962370681944122	31.22605873115291	22.389214176999484	18.42235640990348
8	25.816484300194283	34.06696404843256	20.58558240523832	19.530969246134845
9	26.65702611436826	15.244315022365498	19.512401171779253	38.58625769148699
10	15.232655405370565	27.444679467932907	32.87152738252803	24.451137744168495
11	35.05940002229162	22.754506218845776	23.316318455992104	18.8697753028705
12	23.6975451213993	24.559019460617666	29.911909003178323	21.831526414804713
13	28.7921774472151	21.246791735598162	25.320608292086817	24.640422525099922
14	22.5398537720041	20.44479169352008	26.88973013062162	30.1256244038542
15	24.125705351409472	27.851341055130746	23.38274379368102	24.640209799778763
16	24.129093829194698	27.213286681387867	24.60067724916415	24.05694224025329
17	23.64783711056249	26.324277926215515	25.907867764189202	24.12001719903279
18	24.212026428537996	25.812088938106804	26.768770917502444	23.20711371585276
19	25.354317591834224	25.737023823453004	25.971549882895623	22.93710870181715
20	24.56537355324499	25.837728617933713	26.064417809400997	23.5324800194203
21	24.653949799418207	25.641310409548595	25.95505412971617	23.749685661317024
22	24.015072337303042	25.661407875296238	26.30908207482622	24.014437712574495
23	23.67683271243344	25.405145112872617	26.322968959236388	24.59505321545755
24	23.547656571370318	26.02026305235779	26.369973458344752	24.06210691792714
25	23.907551976431087	25.703618826416825	26.22779556631197	24.161033630840123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9243.0
1	9243.0
2	8652.0
3	8061.0
4	8061.0
5	8061.0
6	6648.0
7	5235.0
8	5235.0
9	5235.0
10	6890.0
11	8545.0
12	8545.0
13	8545.0
14	11866.5
15	15188.0
16	15188.0
17	15188.0
18	24316.5
19	33445.0
20	33445.0
21	33445.0
22	47275.0
23	61105.0
24	61105.0
25	61105.0
26	81070.5
27	101036.0
28	101036.0
29	101036.0
30	121881.0
31	142726.0
32	142726.0
33	142726.0
34	165053.0
35	187380.0
36	187380.0
37	187380.0
38	209625.0
39	231870.0
40	231870.0
41	231870.0
42	251741.5
43	271613.0
44	271613.0
45	271613.0
46	287107.5
47	302602.0
48	302602.0
49	302602.0
50	304942.5
51	307283.0
52	307283.0
53	307283.0
54	287367.0
55	267451.0
56	267451.0
57	267451.0
58	239901.5
59	212352.0
60	212352.0
61	212352.0
62	183067.5
63	153783.0
64	153783.0
65	153783.0
66	126165.5
67	98548.0
68	98548.0
69	98548.0
70	76743.5
71	54939.0
72	54939.0
73	54939.0
74	42813.5
75	30688.0
76	30688.0
77	30688.0
78	24547.0
79	18406.0
80	18406.0
81	18406.0
82	14014.0
83	9622.0
84	9622.0
85	9622.0
86	7058.5
87	4495.0
88	4495.0
89	4495.0
90	3164.5
91	1834.0
92	1834.0
93	1834.0
94	1009.5
95	185.0
96	185.0
97	185.0
98	109.5
99	34.0
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22422151982784203
2	0.6282143179429626
3	0.6258893496354332
4	0.6328642545580215
5	0.6473657517982054
6	0.6626947801308997
7	0.6563503750883193
8	0.6523703445957688
9	0.6452772209456789
10	0.6465776269481953
11	0.6518580634432624
12	0.6512275635632543
13	0.6480750641632144
14	0.6409031280281234
15	0.6465776269481953
16	0.6536707505982853
17	0.6540254067807898
18	0.6484297203457189
19	0.6468140644031983
20	0.6539860005382893
21	0.6503212199857428
22	0.6499665638032383
23	0.6540254067807898
24	0.6591088120633543
25	0.6455530646431824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2537669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.36180515633711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.46630252483257	58.70783531054514
2	6.733325452237206	8.936712634311418
3	1.753931966110402	3.491822741774693
4	0.7668070458912009	2.0354679908775326
5	0.47279250023307223	1.5687681889922305
6	0.3133413728204612	1.2476339478318381
7	0.22446039077497487	1.0426917702546918
8	0.1801657075969206	0.956489726672036
9	0.14098245786689406	0.8420265359481897
>10	0.84174666292895	10.407891014684868
>50	0.06269761518814554	2.88285524137325
>100	0.04003089468162979	5.225343956121853
>500	0.0026364559448160733	1.2100868138410612
>1k	6.591139862029092E-4	0.9586216123830784
>5k	1.1983890658234712E-4	0.4857525143882398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6654	0.2622091375983235	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	5562	0.21917752078777808	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	4178	0.16463928116708682	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4001	0.1576643762444984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.9406242500499473E-5	0.0	0.0	0.0	0.0
9	3.9406242500499473E-5	0.0	0.0	0.0	3.9406242500499473E-5
10	3.9406242500499473E-5	0.0	0.0	0.0	3.9406242500499473E-5
11	3.9406242500499473E-5	0.0	0.0	0.0	3.9406242500499473E-5
12	3.9406242500499473E-5	0.0	0.0	0.0	3.9406242500499473E-5
13	3.9406242500499473E-5	0.0	0.0	3.9406242500499473E-5	1.1821872750149843E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	985	0.0	15.337716	1
GTATCAA	3675	0.0	14.065052	1
TAGATCG	70	1.0900374E-4	12.215944	5
GTCGGCC	80	3.774613E-4	10.6868305	12
ATTAGCC	120	1.5219866E-6	10.293064	3
CGGTCCA	195	1.4551915E-11	10.229925	10
CCGACCA	215	1.8189894E-12	10.161751	9
GCCGTTA	75	0.0026494889	10.1329	15
CGTCGTA	180	3.947207E-10	10.026951	10
CCGCGCA	95	1.6464427E-4	9.998978	9
AAAACCG	105	4.100841E-5	9.953732	5
CGACCAT	240	0.0	9.895017	10
TTAATAC	320	0.0	9.798205	3
ATCGCCA	175	2.575689E-9	9.771204	16
GTATTAG	410	0.0	9.733416	1
TATCAAC	5350	0.0	9.590467	2
TCGCACG	120	1.7012331E-5	9.4999695	18
AACGTCT	140	1.059454E-6	9.499782	6
ATACCGT	210	6.730261E-11	9.499781	6
GATAACG	90	0.001115062	9.499028	11
>>END_MODULE