FastQCFastQC Report
Thu 2 Feb 2017
SRR4050245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537669
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113060.4455269777106471No Hit
TATCAACGCAGAGTACTTTTTTTTT80140.31580162739900275No Hit
GGTATCAACGCAGAGTACTTTTTTT40620.16006815703702887No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38310.1509653150194135No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37340.14714290949686504No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29320.11553910301146447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG350.002172348616.28387310
GGTATCA13950.015.5757841
GTATCAA43450.012.8747151
AACCGCG1600.012.4656197
CGATAAC1301.9826984E-1012.42167210
ATTCCGA1551.8189894E-1212.2547466
AATTCCG1353.7107384E-1011.9646775
CGAACGA1406.85759E-1011.53145516
ACGAACG1354.7439244E-911.25754315
AGAACCG2000.010.9265655
GATAACG1408.376446E-910.85570111
CGGAATT750.00265191210.13178815
AACGAAC1502.4525434E-810.13178814
GAACCGC2250.010.13058956
CCGATAA1606.188202E-910.0926089
GGACTGT3750.09.8773256
TCCGATA1651.0270014E-89.7854228
ACCGTCG1752.586603E-99.7689768
GTTCTAA2053.8198777E-119.7623581
CAATACC1502.644265E-79.5013614
Produced by FastQC (version 0.11.5)