##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050245_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2537669
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.577059104240938	31.0	31.0	34.0	27.0	34.0
2	30.590162467997207	31.0	31.0	34.0	26.0	34.0
3	30.76663426159992	31.0	31.0	34.0	27.0	34.0
4	34.44471363286544	37.0	35.0	37.0	30.0	37.0
5	34.38557313818311	37.0	35.0	37.0	31.0	37.0
6	34.351783073363784	37.0	35.0	37.0	30.0	37.0
7	34.293539070698344	37.0	35.0	37.0	30.0	37.0
8	34.27071615722933	37.0	35.0	37.0	30.0	37.0
9	35.843687651935696	39.0	37.0	39.0	30.0	39.0
10	35.73088531246589	39.0	37.0	39.0	30.0	39.0
11	35.71943819308192	39.0	37.0	39.0	30.0	39.0
12	35.6468345556493	39.0	37.0	39.0	30.0	39.0
13	35.64380421560101	39.0	37.0	39.0	30.0	39.0
14	36.79418001323262	40.0	37.0	41.0	30.0	41.0
15	36.77358512871458	40.0	37.0	41.0	30.0	41.0
16	36.749042920885266	40.0	37.0	41.0	30.0	41.0
17	36.728730973188384	40.0	37.0	41.0	30.0	41.0
18	36.69107988472886	40.0	37.0	41.0	30.0	41.0
19	36.75006393662846	40.0	37.0	41.0	30.0	41.0
20	36.699914764297475	40.0	37.0	41.0	30.0	41.0
21	36.6552075940558	40.0	37.0	41.0	30.0	41.0
22	36.59884169290794	40.0	37.0	41.0	30.0	41.0
23	36.50195868728349	40.0	37.0	41.0	30.0	41.0
24	36.44746024796772	40.0	37.0	41.0	30.0	41.0
25	36.22200846524901	40.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.810524964320674
1101	2	0.9785495798132935
1101	3	0.8918266484710387
1101	4	0.7647176509260873
1101	5	0.925621232270835
1101	6	0.9846237226738239
1101	7	0.9324040148340771
1101	8	0.9324646166719432
1101	9	1.0767574486840843
1101	10	1.1090944297692786
1101	11	1.1355764366369243
1101	12	1.1392951491196968
1101	13	1.170248615567516
1101	14	1.301888073503818
1101	15	1.3560852864872501
1101	16	1.3653626940582058
1101	17	1.3351872685542645
1101	18	1.3059662791449114
1101	19	1.2762860749708125
1101	20	1.3422705217675386
1101	21	1.4013055951187
1101	22	1.4398018598945939
1101	23	1.3953038666580468
1101	24	1.453224599015293
1101	25	1.5490323959344536
1102	1	0.8251639063433025
1102	2	1.0425368152841834
1102	3	0.907053389062785
1102	4	0.8469020681244785
1102	5	0.8807488680800333
1102	6	0.9776837645711609
1102	7	0.8846147822229256
1102	8	0.9800747204544606
1102	9	1.07190176947055
1102	10	1.1882036308752504
1102	11	1.1730730705636532
1102	12	1.1531233889889165
1102	13	1.1931813724858245
1102	14	1.3034862471641162
1102	15	1.4427325953353787
1102	16	1.3439091129987375
1102	17	1.2941299454327222
1102	18	1.342168950258376
1102	19	1.3470214181712166
1102	20	1.3176496712034904
1102	21	1.3973317322825523
1102	22	1.3904393436015354
1102	23	1.4539399938216562
1102	24	1.4787043513734446
1102	25	1.4273249054890442
1103	1	0.742745380118496
1103	2	0.9320268925152284
1103	3	0.9241230948758705
1103	4	0.8301312957411895
1103	5	0.8626184796911573
1103	6	0.9247069945817756
1103	7	0.8732313365189484
1103	8	0.9195003373195263
1103	9	1.0658545131028134
1103	10	1.044890695828478
1103	11	1.1297604996997421
1103	12	1.1334408229079358
1103	13	1.1547555286134426
1103	14	1.1792340359671982
1103	15	1.145501416912488
1103	16	1.1520912699809713
1103	17	1.2026892441136567
1103	18	1.3111633321308105
1103	19	1.2434452335450743
1103	20	1.230757158560273
1103	21	1.310008573408993
1103	22	1.2481726755590685
1103	23	1.335882077821644
1103	24	1.434919860221875
1103	25	1.4069733051481634
1104	1	0.6874805438746563
1104	2	0.7949107892144092
1104	3	0.7474983518202514
1104	4	0.6357525849596186
1104	5	0.7146906386204321
1104	6	0.7543164505938122
1104	7	0.7880430903478768
1104	8	0.7921281900903949
1104	9	0.9073612682164622
1104	10	0.9029669222790631
1104	11	0.9267569460671865
1104	12	0.9172116594913149
1104	13	0.90456955938253
1104	14	1.0378624347448735
1104	15	1.024279015491949
1104	16	1.074930384945226
1104	17	0.9874170264542386
1104	18	0.9743882536710089
1104	19	0.94137470919501
1104	20	1.0683978470794457
1104	21	0.9858504390546159
1104	22	1.0703162627320353
1104	23	1.166287745968802
1104	24	1.1136966558501484
1104	25	1.1239443381104621
1105	1	0.5204987968081625
1105	2	0.727158996515584
1105	3	0.6662198316116488
1105	4	0.6214680347640495
1105	5	0.6751820010715406
1105	6	0.7354849642835148
1105	7	0.6709717083400548
1105	8	0.6716262344189445
1105	9	0.6731736867301521
1105	10	0.7132708662164831
1105	11	0.6055949512823133
1105	12	0.8002584326334272
1105	13	0.7740896050148649
1105	14	0.9597170631672967
1105	15	0.9191372293120281
1105	16	0.89505342927378
1105	17	0.9210586496615392
1105	18	0.9238626669695762
1105	19	0.8611188421806659
1105	20	0.8861211195696725
1105	21	0.8613108367078084
1105	22	0.8319402848963122
1105	23	0.8465802818227743
1105	24	0.9122869426819591
1105	25	0.9877277474976793
1106	1	0.4868833232226031
1106	2	0.6311500111970005
1106	3	0.4813045573059007
1106	4	0.4854307662955506
1106	5	0.5200932081693566
1106	6	0.5624245307913966
1106	7	0.5490974880833548
1106	8	0.559274097161385
1106	9	0.6363472595114388
1106	10	0.645985533518207
1106	11	0.6406777637833585
1106	12	0.633089831478685
1106	13	0.5648618594320993
1106	14	0.7136071669413084
1106	15	0.7118515755183878
1106	16	0.6970006719676931
1106	17	0.6069520607177878
1106	18	0.6946645959499733
1106	19	0.6857229081844949
1106	20	0.7078747952753943
1106	21	0.6513271122290334
1106	22	0.6136023949076019
1106	23	0.7935301768981802
1106	24	0.7619058091751896
1106	25	0.7872673101256638
1107	1	0.2090172442573781
1107	2	0.4993471273773409
1107	3	0.3970891459700603
1107	4	0.3212834419853152
1107	5	0.4227406317860982
1107	6	0.4917797160394102
1107	7	0.45949521582627995
1107	8	0.32944366302642436
1107	9	0.3883831722623938
1107	10	0.4710753490149884
1107	11	0.47903534273203263
1107	12	0.4323753418313885
1107	13	0.5202022480102002
1107	14	0.49314025049118726
1107	15	0.5292125515772454
1107	16	0.5125158141415085
1107	17	0.5486526743492632
1107	18	0.5474942883894371
1107	19	0.501738979287957
1107	20	0.5713451543511852
1107	21	0.5168099797872543
1107	22	0.5686802936232311
1107	23	0.614389509227415
1107	24	0.6518835065882556
1107	25	0.6418075830530512
1108	1	0.3120224016098696
1108	2	0.510617283644919
1108	3	0.42414980161883875
1108	4	0.387291953525569
1108	5	0.36159714909648244
1108	6	0.5255786325332963
1108	7	0.5104867304042884
1108	8	0.41776156649332563
1108	9	0.5525943454546081
1108	10	0.5142499686928872
1108	11	0.6248045105237949
1108	12	0.6094636979077066
1108	13	0.5533582534026138
1108	14	0.6872057289777729
1108	15	0.6392323462073293
1108	16	0.6578727403031692
1108	17	0.5847066768726847
1108	18	0.7530433897202613
1108	19	0.6674312338164583
1108	20	0.6304922222747393
1108	21	0.6886869888844487
1108	22	0.6838810476374135
1108	23	0.792660721275567
1108	24	0.8160237335358715
1108	25	0.9081109044925313
1109	1	0.2134452362581385
1109	2	0.2647009488740153
1109	3	0.24692406520621546
1109	4	0.3069583309177233
1109	5	0.3334440293863281
1109	6	0.37488805837288197
1109	7	0.36529292197881347
1109	8	0.3979050158554358
1109	9	0.4527775893108199
1109	10	0.492729264666913
1109	11	0.47556305869569826
1109	12	0.4422811766878212
1109	13	0.493838097128382
1109	14	0.5510349756211426
1109	15	0.5883700771732308
1109	16	0.5874613435472256
1109	17	0.49611278199482456
1109	18	0.5269819557325945
1109	19	0.47918226855029644
1109	20	0.4547013094541583
1109	21	0.3829128275880578
1109	22	0.4099655795901427
1109	23	0.6046812322685469
1109	24	0.5317163810136307
1109	25	0.506742515047641
1110	1	-0.08979433879441245
1110	2	0.030957856346347512
1110	3	0.02438935057196545
1110	4	0.09515410600623397
1110	5	0.14780453372374325
1110	6	-0.06958506639992379
1110	7	0.04630362378820507
1110	8	0.09697077615334138
1110	9	0.1545692124731275
1110	10	0.12688194043597179
1110	11	0.07342086690629657
1110	12	0.1753950615209945
1110	13	0.09143338356040687
1110	14	0.06235754696917439
1110	15	0.025815817686989817
1110	16	0.1895531072255423
1110	17	-0.014733660711911511
1110	18	0.08739539586232326
1110	19	0.0780857650518243
1110	20	0.11633660195303719
1110	21	0.04911022777787366
1110	22	0.1661840202318956
1110	23	0.07098536550855528
1110	24	0.12815759214694822
1110	25	0.14188809322722307
1111	1	-0.3590712593922589
1111	2	-0.1987798593191492
1111	3	-0.3266882998740641
1111	4	-0.14789516938741798
1111	5	-0.058089586603351506
1111	6	-0.28444283811646187
1111	7	-0.2521339488782175
1111	8	-0.2323352397284637
1111	9	-0.30433558509574254
1111	10	-0.32947687902834133
1111	11	-0.24737631549730565
1111	12	-0.23266027154275548
1111	13	-0.21587432074421997
1111	14	-0.3757488182632329
1111	15	-0.26813888767399163
1111	16	-0.3099875706006614
1111	17	-0.42042476266510675
1111	18	-0.3598281598092896
1111	19	-0.35401213914858687
1111	20	-0.4485402059031145
1111	21	-0.4865269345051857
1111	22	-0.40522959865366914
1111	23	-0.4461237942809433
1111	24	-0.46898122145506704
1111	25	-0.45640144685040696
1112	1	-0.6419323506517571
1112	2	-0.4903822245518761
1112	3	-0.5142387109276747
1112	4	-0.44373331557190454
1112	5	-0.5042747045959857
1112	6	-1.4277688556982255
1112	7	-0.6695717789664712
1112	8	-0.5071062757996287
1112	9	-0.649428339256211
1112	10	-0.6876955116725512
1112	11	-0.7152201349469394
1112	12	-0.643417867860947
1112	13	-0.5750125228859275
1112	14	-0.6995031166121564
1112	15	-0.7376170435557015
1112	16	-0.6978232349766884
1112	17	-0.8332270629011944
1112	18	-0.8990911145379599
1112	19	-0.8541031322575066
1112	20	-0.9320926160824072
1112	21	-0.8816762448852415
1112	22	-1.0483207004533384
1112	23	-0.887557612613179
1112	24	-0.9013695297281785
1112	25	-0.764875110689303
1113	1	-0.6755453645080927
1113	2	-0.8210889591383541
1113	3	-0.5465464752348161
1113	4	-0.4460272477464784
1113	5	-0.6025515995585238
1113	6	-1.2198998865605049
1113	7	-0.7278485796871692
1113	8	-0.7337738156312668
1113	9	-0.7160621961958071
1113	10	-0.7730213817657372
1113	11	-0.8309135118228568
1113	12	-0.87419087159914
1113	13	-0.7693627671329466
1113	14	-0.8993692396461697
1113	15	-0.8822231222831931
1113	16	-0.9139546436630894
1113	17	-0.9573874269660081
1113	18	-0.8161617181719691
1113	19	-0.9034819499892848
1113	20	-0.9920444584997625
1113	21	-0.9286538232510821
1113	22	-0.9918109878674031
1113	23	-1.0495634667452194
1113	24	-1.055106518731229
1113	25	-1.0122615058937612
1114	1	-1.1202583437999536
1114	2	-1.0800666706977644
1114	3	-1.132934927036029
1114	4	-1.0026801896941464
1114	5	-1.319609600316916
1114	6	-1.8173407860783541
1114	7	-1.500294059690873
1114	8	-1.6680745837685649
1114	9	-1.7457334299574754
1114	10	-1.7597622663274493
1114	11	-1.6296915060559272
1114	12	-1.6426116585089616
1114	13	-1.8207111671658396
1114	14	-2.0626489706637514
1114	15	-1.9155146092025461
1114	16	-1.9472404304222692
1114	17	-1.906365076095092
1114	18	-2.201967939326785
1114	19	-1.9272923333467702
1114	20	-1.8648001629350546
1114	21	-2.052268202529426
1114	22	-1.771375262194944
1114	23	-1.8842982342207577
1114	24	-1.9612716762578657
1114	25	-2.014124752536709
1115	1	-1.4342004626479223
1115	2	-2.0391474339500952
1115	3	-1.61707600666686
1115	4	-1.3516453629552672
1115	5	-1.4216151181817125
1115	6	-1.9778465710831128
1115	7	-1.5787212342528676
1115	8	-1.5319248620703334
1115	9	-1.6199122945479303
1115	10	-1.700090822337522
1115	11	-1.7022678061026895
1115	12	-1.7049843954233026
1115	13	-1.7302562805138422
1115	14	-1.8445801793007561
1115	15	-1.9172830455074887
1115	16	-1.897665968837984
1115	17	-1.8980854712475406
1115	18	-1.947873265803203
1115	19	-1.9617510494178632
1115	20	-2.02674302608645
1115	21	-2.081641047070484
1115	22	-2.1922862246227552
1115	23	-2.160894708633812
1115	24	-2.0565399268998448
1115	25	-2.129515620561307
1116	1	-1.8013794889301558
1116	2	-1.915680722540646
1116	3	-1.9117672988085062
1116	4	-1.7886425763307017
1116	5	-1.912146089099629
1116	6	-2.173940110368463
1116	7	-2.154018828733591
1116	8	-2.088065905043557
1116	9	-2.339708510351059
1116	10	-2.421262506370475
1116	11	-2.319467083457141
1116	12	-2.312960224051608
1116	13	-2.4117832973697944
1116	14	-2.6665183744404715
1116	15	-2.5256287209760657
1116	16	-2.645502570221531
1116	17	-2.6413504011018034
1116	18	-2.7091734603803843
1116	19	-2.607678735310188
1116	20	-2.6273204416028904
1116	21	-2.880683527593831
1116	22	-2.774040284561565
1116	23	-2.774620878962857
1116	24	-3.020034208009889
1116	25	-3.148942143573521
1201	1	0.9925917426279973
1201	2	1.092880582303927
1201	3	1.0067722292515242
1201	4	0.9310683615232307
1201	5	1.032823622613968
1201	6	1.0497488607979335
1201	7	1.0059105223877367
1201	8	1.105170476398328
1201	9	1.2241072738148802
1201	10	1.171955660519508
1201	11	1.211921988680551
1201	12	1.2182800597106294
1201	13	1.2533663691518626
1201	14	1.4550128704977752
1201	15	1.4815700609141373
1201	16	1.432299166087617
1201	17	1.5391401381916694
1201	18	1.5880059526455952
1201	19	1.5003979445400546
1201	20	1.540063688092168
1201	21	1.642050839697184
1201	22	1.5207689094739933
1201	23	1.6633716043539195
1201	24	1.674848154532306
1201	25	1.7476406265153912
1202	1	0.8369701138081673
1202	2	0.9915541048540923
1202	3	0.9385514392565817
1202	4	0.819110862573261
1202	5	0.8397397467519738
1202	6	0.8696722614625685
1202	7	0.9262533986085302
1202	8	0.9832826596813433
1202	9	1.1091752946179412
1202	10	1.0752842635898574
1202	11	1.1437101921809472
1202	12	1.0742271426152001
1202	13	1.1476531171260405
1202	14	1.216858408319844
1202	15	1.2693050317578596
1202	16	1.3744136378207443
1202	17	1.3104626111693705
1202	18	1.2803967771345413
1202	19	1.3169176227811903
1202	20	1.3068526505173423
1202	21	1.4265523334063133
1202	22	1.3377555394598204
1202	23	1.3519037460017884
1202	24	1.410180631432432
1202	25	1.4695002951697802
1203	1	0.7082358985377404
1203	2	0.8865747639884063
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2211	11	-0.3336804326669949
2211	12	-0.41030651033695165
2211	13	-0.3499546075914779
2211	14	-0.4469151978257173
2211	15	-0.4063564374208326
2211	16	-0.3344963284104452
2211	17	-0.49613466886189883
2211	18	-0.4565405923594881
2211	19	-0.47783269343155155
2211	20	-0.5259654973715868
2211	21	-0.3980448093298463
2211	22	-0.4826024851633477
2211	23	-0.5296349666171878
2211	24	-0.45345303627499334
2211	25	-0.46763465303813945
2212	1	-0.3736152846360703
2212	2	-0.43273370072549966
2212	3	-0.42840088277250743
2212	4	-0.44375949269889503
2212	5	-0.48098095569390864
2212	6	-0.3538805615036651
2212	7	-0.46524437536536567
2212	8	-0.46930931684623545
2212	9	-0.6261354557137082
2212	10	-0.5731995706416129
2212	11	-0.6546319846616697
2212	12	-0.6614727299269632
2212	13	-0.6045325338932983
2212	14	-0.7599636263608645
2212	15	-0.7740331049754587
2212	16	-0.7431756483347272
2212	17	-0.7093698783831712
2212	18	-0.7183025729370982
2212	19	-0.8425419284038895
2212	20	-0.6505087225008666
2212	21	-0.6661952217879303
2212	22	-0.6356854555695577
2212	23	-0.7293142838864384
2212	24	-0.844799027585978
2212	25	-0.7652571669394064
2213	1	-0.7951577360175577
2213	2	-1.084747786211711
2213	3	-0.7924457123888153
2213	4	-0.7580954745776651
2213	5	-0.8964651783829325
2213	6	-0.7537520356220426
2213	7	-0.895922680495751
2213	8	-0.8749497154216712
2213	9	-0.8840472115418123
2213	10	-0.9483077022246604
2213	11	-0.9135709355279147
2213	12	-0.9568712745616565
2213	13	-1.0386393692173712
2213	14	-1.1350143371032715
2213	15	-1.3520508671554978
2213	16	-1.3808877644401036
2213	17	-1.23529197788271
2213	18	-1.3899006851334619
2213	19	-1.2741046464820798
2213	20	-1.1995951811266181
2213	21	-1.2360752848320544
2213	22	-1.2263210624748453
2213	23	-1.244925272431317
2213	24	-1.2945155314356
2213	25	-1.3646988427655415
2214	1	-1.2440100495826236
2214	2	-1.276650461911057
2214	3	-1.363175872594109
2214	4	-1.2620949082085886
2214	5	-1.326252947791815
2214	6	-1.3539965124276563
2214	7	-1.2322148392649623
2214	8	-1.3533908678336886
2214	9	-1.4947839823087534
2214	10	-1.2652685710313136
2214	11	-1.5591041159006807
2214	12	-1.5563104727373513
2214	13	-1.4452715423123834
2214	14	-1.6482494881072611
2214	15	-1.7597846544023739
2214	16	-1.8198218245260804
2214	17	-1.7084812845540256
2214	18	-1.85605728355236
2214	19	-1.7752842898098393
2214	20	-1.8095308377421375
2214	21	-2.015817771016941
2214	22	-1.914206331530842
2214	23	-1.9888859018900433
2214	24	-2.005222361794033
2214	25	-2.0034016208672227
2215	1	-1.5692119384868732
2215	2	-3.3852350322923606
2215	3	-1.9933947732304347
2215	4	-1.5725217845156862
2215	5	-1.5634105393051172
2215	6	-1.3976638227011335
2215	7	-1.4051909336566624
2215	8	-1.4954174266479043
2215	9	-1.710480705265006
2215	10	-1.7796204084321516
2215	11	-1.7376543952676613
2215	12	-1.8079173345075503
2215	13	-1.768716518388942
2215	14	-2.099882881936658
2215	15	-2.0998527459223695
2215	16	-2.165234899703215
2215	17	-2.2313514736674094
2215	18	-2.0394845567255473
2215	19	-1.9566612367947727
2215	20	-1.9337294134833627
2215	21	-2.0167981194564533
2215	22	-2.032941532661688
2215	23	-2.1969008133532313
2215	24	-2.2942675198047056
2215	25	-2.2234638708750225
2216	1	-1.874286079332645
2216	2	-2.6746662968351345
2216	3	-2.096231276602172
2216	4	-1.9274237514158656
2216	5	-2.000698051079695
2216	6	-1.8639935233305707
2216	7	-1.9942173398998406
2216	8	-2.043628002869191
2216	9	-2.402445609228124
2216	10	-2.365999016443233
2216	11	-2.33808171103842
2216	12	-2.4172122322948653
2216	13	-2.610525779600522
2216	14	-2.676267291461123
2216	15	-2.77473500015342
2216	16	-2.616028708926997
2216	17	-2.6765945446018264
2216	18	-3.0829169940473733
2216	19	-2.8536138827215467
2216	20	-3.0067080851239396
2216	21	-3.0093540915842354
2216	22	-2.978489962822067
2216	23	-3.058504463939933
2216	24	-2.9771524315031783
2216	25	-3.152559084314575
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41434.0
3	7.0
4	767.0
5	5933.0
6	7083.0
7	5731.0
8	4606.0
9	3972.0
10	3496.0
11	3286.0
12	2920.0
13	2759.0
14	2790.0
15	2857.0
16	2886.0
17	3217.0
18	3408.0
19	4371.0
20	5859.0
21	7777.0
22	9478.0
23	11657.0
24	13796.0
25	15694.0
26	18129.0
27	20599.0
28	23973.0
29	29272.0
30	37501.0
31	49013.0
32	63842.0
33	84207.0
34	113049.0
35	157334.0
36	228378.0
37	401309.0
38	1145279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05859900209409	18.857425013747793	12.943798707806334	26.14017727635178
2	16.05148360801425	21.377031633200378	37.556085313126005	25.015399445659366
3	18.502216010047015	25.287340468753015	29.176027291187307	27.03441623001266
4	12.97765784268949	17.286257585209103	37.307268993710366	32.42881557839104
5	14.448477929024689	36.57003387286008	34.12694595481096	14.854542243304275
6	31.53253553879505	37.94321280243886	16.8159493753692	13.708302283396891
7	27.670606131062392	31.541592941278466	22.407775943126694	18.380024984532447
8	25.80126013778841	34.41971517843808	20.273882345894386	19.505142337879125
9	26.594507467156923	15.272331025894156	19.458116333555996	38.67504517339292
10	15.135932098782462	27.82189015001184	32.74457865798458	24.297599093221113
11	35.192006447176304	22.7551432585432	23.203687028210336	18.84916326607016
12	23.70586306535238	24.741038619965604	29.914945906195367	21.63815240848665
13	28.92715723526743	21.28403290601339	25.333436855980423	24.455373002738753
14	22.66048599674731	20.46718361405255	26.78743484464978	30.08489554455036
15	24.0924541182072	27.814393348184513	23.62697523068992	24.466177302918368
16	24.056919979333642	27.219968653656	24.704418636453738	24.018692730556616
17	23.53879553758019	26.278725083712732	26.13992962494882	24.042549753758255
18	24.205243796955074	25.743232459761238	26.93369692217058	23.11782682111311
19	25.172370036775426	25.568970871276548	26.521813834055024	22.736845257893005
20	24.44870573232544	25.596613143104396	26.58409173486172	23.37058938970844
21	24.51021924129547	25.415281671963637	26.43206317743235	23.642435909308542
22	23.916052060395607	25.429094743678053	26.77328161130613	23.88157158462021
23	23.584329420443602	25.159324021633072	26.806394713736935	24.449951844186387
24	23.50943674310298	25.81383609035534	26.834374942120075	23.842352224421603
25	23.76491117038821	25.39762792642371	26.83945648091847	23.998004422269606
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	565.0
1	565.0
2	1059.0
3	1553.0
4	1553.0
5	1553.0
6	3909.0
7	6265.0
8	6265.0
9	6265.0
10	7986.5
11	9708.0
12	9708.0
13	9708.0
14	12405.0
15	15102.0
16	15102.0
17	15102.0
18	24802.5
19	34503.0
20	34503.0
21	34503.0
22	49227.5
23	63952.0
24	63952.0
25	63952.0
26	85688.5
27	107425.0
28	107425.0
29	107425.0
30	127641.5
31	147858.0
32	147858.0
33	147858.0
34	168959.5
35	190061.0
36	190061.0
37	190061.0
38	210921.5
39	231782.0
40	231782.0
41	231782.0
42	251593.5
43	271405.0
44	271405.0
45	271405.0
46	286589.5
47	301774.0
48	301774.0
49	301774.0
50	304584.0
51	307394.0
52	307394.0
53	307394.0
54	287293.5
55	267193.0
56	267193.0
57	267193.0
58	239449.5
59	211706.0
60	211706.0
61	211706.0
62	182209.0
63	152712.0
64	152712.0
65	152712.0
66	125146.0
67	97580.0
68	97580.0
69	97580.0
70	75865.5
71	54151.0
72	54151.0
73	54151.0
74	42406.0
75	30661.0
76	30661.0
77	30661.0
78	24499.5
79	18338.0
80	18338.0
81	18338.0
82	13905.5
83	9473.0
84	9473.0
85	9473.0
86	6941.0
87	4409.0
88	4409.0
89	4409.0
90	3153.0
91	1897.0
92	1897.0
93	1897.0
94	1036.0
95	175.0
96	175.0
97	175.0
98	101.0
99	27.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3220278137140817
2	0.009457498200119874
3	0.0
4	0.0
5	0.04433202281306191
6	0.0035465618250449527
7	0.0039012180075494477
8	3.940624250049948E-4
9	0.008826998320111882
10	0.013240497480167824
11	0.004886374070061934
12	0.008866404562612382
13	0.015053184635190798
14	0.003152499400039958
15	0.01958490252274824
16	0.008354123410105888
17	0.004807561585060936
18	1.576249700019979E-4
19	0.004137655462552445
20	0.00374359303754745
21	0.0021279370950269714
22	3.9406242500499473E-5
23	0.002876655702536462
24	0.003467749340043954
25	0.0022855620650289693
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2537669.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.48700202625493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.08199880805397	57.682260344196145
2	7.084010779949364	9.278212566011115
3	1.7195254314080926	3.3781969623245587
4	0.7721862062586256	2.022726386156189
5	0.47750538419417093	1.5635198031135655
6	0.3142805963522989	1.2348776430081363
7	0.22769778873583196	1.0437871886622037
8	0.18959241276473351	0.9932670975109331
9	0.13828220572497202	0.8150118377855617
>10	0.8853833164921369	10.818257069435221
>50	0.06342806816565247	2.871641532445698
>100	0.042378199496771934	5.379692718075652
>500	0.0030688858479761064	1.3351248751766402
>1k	5.415680908173023E-4	0.8220942459152043
>5k	6.0174232313033594E-5	0.3158020940832349
>10k+	6.0174232313033594E-5	0.4455276360999568
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11306	0.4455269777106471	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8014	0.31580162739900275	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4062	0.16006815703702887	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3831	0.1509653150194135	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3734	0.14714290949686504	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2932	0.11553910301146447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
2	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
3	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
4	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
5	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
6	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
7	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
8	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
9	0.0	0.0	0.0	3.9406242500499473E-5	3.9406242500499473E-5
10	0.0	7.881248500099895E-5	0.0	3.9406242500499473E-5	3.9406242500499473E-5
11	0.0	7.881248500099895E-5	0.0	3.9406242500499473E-5	7.881248500099895E-5
12	0.0	7.881248500099895E-5	0.0	3.9406242500499473E-5	7.881248500099895E-5
13	0.0	7.881248500099895E-5	0.0	3.9406242500499473E-5	1.1821872750149843E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTACG	35	0.0021723486	16.283873	10
GGTATCA	1395	0.0	15.575784	1
GTATCAA	4345	0.0	12.874715	1
AACCGCG	160	0.0	12.465619	7
CGATAAC	130	1.9826984E-10	12.421672	10
ATTCCGA	155	1.8189894E-12	12.254746	6
AATTCCG	135	3.7107384E-10	11.964677	5
CGAACGA	140	6.85759E-10	11.531455	16
ACGAACG	135	4.7439244E-9	11.257543	15
AGAACCG	200	0.0	10.926565	5
GATAACG	140	8.376446E-9	10.855701	11
CGGAATT	75	0.002651912	10.131788	15
AACGAAC	150	2.4525434E-8	10.131788	14
GAACCGC	225	0.0	10.1305895	6
CCGATAA	160	6.188202E-9	10.092608	9
GGACTGT	375	0.0	9.877325	6
TCCGATA	165	1.0270014E-8	9.785422	8
ACCGTCG	175	2.586603E-9	9.768976	8
GTTCTAA	205	3.8198777E-11	9.762358	1
CAATACC	150	2.644265E-7	9.501361	4
>>END_MODULE