FastQCFastQC Report
Thu 2 Feb 2017
SRR4050244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504118
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN81100.3238665270566323No Hit
GTATCAACGCAGAGTACTTTTTTTT63140.25214466730401686No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN36750.14675825979446655No Hit
TATCAACGCAGAGTACTTTTTTTTT36680.1464787202520009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.014.7835831
GTATCAA37800.013.4259081
CTATACG550.003070017312.090554
CTCGACG700.00149181610.85725713
TAGATCG700.001492316710.8568215
CGAACGT1502.1354936E-910.7663484
TACCGTC1701.5625119E-910.0583237
ATTAGAC1054.10824E-59.9520863
AACCGCG1151.02754E-59.912557
GTCTTAA2800.09.5037321
TCGAACG1901.0531949E-99.499723
CGAACCT2700.09.49971916
TTAGTAC900.00111431029.4997193
TAAGGCG800.00451816069.4997195
ATCGATC800.00451816069.4997198
TATCAAC52850.09.3650952
CGGTTCT2650.09.32085312
TCGCCAG2054.2382453E-109.26801817
ATTCGAA1856.6556822E-99.2468751
CGTCGTA1651.06634616E-79.21203310
Produced by FastQC (version 0.11.5)