##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050244_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504118 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.786325564530106 31.0 30.0 34.0 26.0 34.0 2 29.8455739705557 31.0 31.0 34.0 25.0 34.0 3 29.995114048139904 31.0 31.0 34.0 26.0 34.0 4 33.41950898480024 37.0 35.0 37.0 28.0 37.0 5 33.452974660139816 37.0 35.0 37.0 28.0 37.0 6 33.494571342085315 37.0 35.0 37.0 28.0 37.0 7 33.37792508180525 37.0 35.0 37.0 28.0 37.0 8 33.348687641716566 37.0 35.0 37.0 27.0 37.0 9 34.82081515327952 39.0 35.0 39.0 27.0 39.0 10 34.71107751312039 39.0 35.0 39.0 27.0 39.0 11 34.74022629923989 39.0 35.0 39.0 27.0 39.0 12 34.66009389333889 39.0 35.0 39.0 27.0 39.0 13 34.65133232539361 39.0 35.0 39.0 27.0 39.0 14 35.7202168587902 40.0 36.0 41.0 27.0 41.0 15 35.70671310217809 40.0 36.0 41.0 27.0 41.0 16 35.70357666851163 40.0 36.0 41.0 27.0 41.0 17 35.67024716886345 40.0 36.0 41.0 27.0 41.0 18 35.6105842456306 40.0 36.0 41.0 27.0 41.0 19 35.651603878092004 40.0 36.0 41.0 27.0 41.0 20 35.61949237216457 40.0 36.0 41.0 26.0 41.0 21 35.544430414221694 40.0 36.0 41.0 26.0 41.0 22 35.45579761017652 40.0 36.0 41.0 25.0 41.0 23 35.38304105477457 40.0 36.0 41.0 25.0 41.0 24 35.325202326727414 40.0 36.0 41.0 25.0 41.0 25 34.98943420397921 39.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0483340263328138 1101 2 1.161621561154746 1101 3 1.1465909572427009 1101 4 1.111391133007814 1101 5 1.143442388592014 1101 6 1.1374063877169434 1101 7 1.1396601991896773 1101 8 1.1882019166669338 1101 9 1.3081779263005373 1101 10 1.2794260100388115 1101 11 1.2865155209404904 1101 12 1.3099250017264126 1101 13 1.2445304767080287 1101 14 1.4665870921184236 1101 15 1.5147028654426364 1101 16 1.5172366111968785 1101 17 1.5072470949185544 1101 18 1.5909542327151769 1101 19 1.6256749676424818 1101 20 1.6392584946509672 1101 21 1.6835285284121184 1101 22 1.6571167955281325 1101 23 1.705173759042573 1101 24 1.618269486938324 1101 25 1.5867679939759398 1102 1 1.072019365617475 1102 2 1.128598220279045 1102 3 1.1261312073420875 1102 4 1.1228387676485312 1102 5 1.169722598820485 1102 6 1.1147665763297496 1102 7 1.077829233217841 1102 8 1.0748378111431762 1102 9 1.3124656392629674 1102 10 1.3681745924910658 1102 11 1.3487947354281076 1102 12 1.259528872730911 1102 13 1.28249866997092 1102 14 1.511521673347879 1102 15 1.4311584738539196 1102 16 1.4956424928383 1102 17 1.4713275187441752 1102 18 1.6046853009444817 1102 19 1.5319997797954699 1102 20 1.537746595093644 1102 21 1.5571460607169314 1102 22 1.6012062767975124 1102 23 1.4857048786276579 1102 24 1.4548843478339037 1102 25 1.4703943731822946 1103 1 0.9115032734127126 1103 2 1.0326679232899991 1103 3 0.9598055976604414 1103 4 1.0872028816566797 1103 5 1.1060407154362366 1103 6 1.0677273094758917 1103 7 1.0503614500960197 1103 8 1.1045563137276417 1103 9 1.2711625534963318 1103 10 1.3333106439369686 1103 11 1.292063866368558 1103 12 1.3186124944908997 1103 13 1.2394549531613706 1103 14 1.5687105254389593 1103 15 1.484348431823534 1103 16 1.4784432818849709 1103 17 1.5154715147063982 1103 18 1.4958073593717316 1103 19 1.4531071670563307 1103 20 1.5016332059919932 1103 21 1.522845861355492 1103 22 1.467567621443152 1103 23 1.5306220563354174 1103 24 1.5890313029155578 1103 25 1.5421192287356646 1104 1 0.9171607043679622 1104 2 0.9817153511220589 1104 3 0.9440792334967867 1104 4 1.0233102746875105 1104 5 1.0058589013105035 1104 6 0.909897032404551 1104 7 0.9021003962433483 1104 8 0.9238018408770614 1104 9 1.1293564588191316 1104 10 1.1784016625422424 1104 11 1.111673021769441 1104 12 1.0877765956821719 1104 13 1.1421052007855224 1104 14 1.3266043816831044 1104 15 1.297472511598663 1104 16 1.3663342380264965 1104 17 1.4035097068270375 1104 18 1.3847892002676403 1104 19 1.3717102099332266 1104 20 1.4227872237495447 1104 21 1.4013237536501393 1104 22 1.537941957765824 1104 23 1.598585961212052 1104 24 1.5066985049857848 1104 25 1.4094540344287907 1105 1 0.6728169362717651 1105 2 0.8204789622994078 1105 3 0.7774179602059199 1105 4 0.8612946849928278 1105 5 0.8515603807986452 1105 6 0.7983597499846837 1105 7 0.7006609326287645 1105 8 0.8470580773226573 1105 9 1.0331409054587724 1105 10 1.066424678063811 1105 11 0.8598233777322477 1105 12 0.8564896226717593 1105 13 0.8499607945678065 1105 14 1.0018104654110047 1105 15 0.9325561373097386 1105 16 1.0501637619333408 1105 17 0.9698760476202608 1105 18 1.0383908398538821 1105 19 1.1070850779374553 1105 20 1.0807725424128947 1105 21 0.9946169489130554 1105 22 1.2310038055931471 1105 23 1.0776775464103636 1105 24 1.0859763035801038 1105 25 1.0460639854909957 1106 1 0.5412526360882524 1106 2 0.686310543217985 1106 3 0.6626205116086403 1106 4 0.7477343075170779 1106 5 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0.615476542310077 1107 18 0.6402561895981123 1107 19 0.6617463017094636 1107 20 0.6425235625132757 1107 21 0.61983347098667 1107 22 0.638242999770334 1107 23 0.5389133236017614 1107 24 0.5391181093068198 1107 25 0.5731487429086926 1108 1 0.5576440435644123 1108 2 0.7082103783787481 1108 3 0.6574477591813412 1108 4 0.7410361888568957 1108 5 0.7450106988338163 1108 6 0.6753666682639334 1108 7 0.6434747250525348 1108 8 0.6423861566237647 1108 9 0.6863018009116928 1108 10 0.7379053017667871 1108 11 0.6669592374395137 1108 12 0.6525986295573674 1108 13 0.6575816542255382 1108 14 0.7490126102302241 1108 15 0.8393053227053215 1108 16 0.7445182527088363 1108 17 0.7887651888436267 1108 18 0.7502282933657938 1108 19 0.8281773669202934 1108 20 0.7641702790619362 1108 21 0.7936273186994072 1108 22 0.811714452373117 1108 23 0.7952231895655544 1108 24 0.7543557797533751 1108 25 0.8391235317549359 1109 1 0.3119093915397535 1109 2 0.46433600628585125 1109 3 0.3344330719564006 1109 4 0.4153770413791804 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-2.0549140854519976 2216 19 -2.1714588230161382 2216 20 -2.5710167305205687 2216 21 -2.734369309382558 2216 22 -2.499111753827087 2216 23 -2.445892592460652 2216 24 -2.4269262570177332 2216 25 -2.7983262541338405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104937.0 3 10.0 4 773.0 5 4484.0 6 5810.0 7 5428.0 8 4977.0 9 4363.0 10 4049.0 11 3821.0 12 3658.0 13 3523.0 14 3378.0 15 3499.0 16 3716.0 17 3887.0 18 4350.0 19 5398.0 20 6921.0 21 9091.0 22 11200.0 23 13336.0 24 15465.0 25 17601.0 26 19529.0 27 21733.0 28 24456.0 29 28820.0 30 36340.0 31 46471.0 32 59443.0 33 78271.0 34 106278.0 35 147525.0 36 217894.0 37 380777.0 38 1092906.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.037790010062906 17.879373453981263 12.327136562816971 25.755699973138864 2 15.972660843694406 20.53809892352072 38.76644118563488 24.722799047149994 3 18.391372163316415 24.963659970718883 29.101095448318347 27.54387241764636 4 12.558269902650254 16.641104047767648 38.093556772338 32.707069277244095 5 14.776421399100158 36.10055496993494 34.23905896017641 14.883964670788489 6 31.739507437979302 37.318957154600895 17.05160977916915 13.889925628250646 7 27.912348273816107 31.27734591055823 22.41933245507296 18.3909733605527 8 25.777008574303796 34.12132893262426 20.53352296174263 19.568139531329308 9 26.64907089329851 15.276150153101728 19.48284834362993 38.59193060996983 10 15.240930085919787 27.36709697603849 32.87049138268688 24.521481555354846 11 35.102519830903915 22.695611360534944 23.27914649189168 18.922722316669464 12 23.729335730950947 24.610182688970248 29.84808698026872 21.812394599810084 13 28.81300646896714 21.25508580511372 25.3011263178047 24.630781408114444 14 22.769944270478238 20.468119938136407 26.54804785780223 30.213887933583123 15 24.12095611658483 27.794961355050873 23.328871413034385 24.755211115329914 16 24.10599014954259 27.22248632752329 24.538626807948255 24.132896714985872 17 23.63872720888449 26.311297346454722 25.914269085491597 24.135706359169195 18 24.38246316459296 25.848277260172146 26.525998068481616 23.243261506753278 19 25.35355168704833 25.7446930039444 25.92739187903098 22.97436342997629 20 24.619814569983923 25.8804024260112 25.977457353952044 23.52232565005283 21 24.69425144040784 25.669887683381766 25.853415249720967 23.782445626489427 22 24.063005694550537 25.663288229854373 26.214561911393165 24.059144164201925 23 23.726923214599573 25.404905806535687 26.155211780950072 24.712959197914667 24 23.61335905372967 26.02963369350469 26.318103276293687 24.03890397647195 25 23.95949895380744 25.637147759715994 26.196364028352182 24.206989258124384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11376.0 1 11376.0 2 9275.0 3 7174.0 4 7174.0 5 7174.0 6 6056.5 7 4939.0 8 4939.0 9 4939.0 10 6746.5 11 8554.0 12 8554.0 13 8554.0 14 11698.5 15 14843.0 16 14843.0 17 14843.0 18 23850.0 19 32857.0 20 32857.0 21 32857.0 22 46444.5 23 60032.0 24 60032.0 25 60032.0 26 79550.5 27 99069.0 28 99069.0 29 99069.0 30 119512.0 31 139955.0 32 139955.0 33 139955.0 34 161962.0 35 183969.0 36 183969.0 37 183969.0 38 205122.0 39 226275.0 40 226275.0 41 226275.0 42 247139.0 43 268003.0 44 268003.0 45 268003.0 46 283056.5 47 298110.0 48 298110.0 49 298110.0 50 301177.5 51 304245.0 52 304245.0 53 304245.0 54 284155.0 55 264065.0 56 264065.0 57 264065.0 58 237370.5 59 210676.0 60 210676.0 61 210676.0 62 181810.0 63 152944.0 64 152944.0 65 152944.0 66 125231.5 67 97519.0 68 97519.0 69 97519.0 70 75772.0 71 54025.0 72 54025.0 73 54025.0 74 42330.5 75 30636.0 76 30636.0 77 30636.0 78 24700.0 79 18764.0 80 18764.0 81 18764.0 82 14153.0 83 9542.0 84 9542.0 85 9542.0 86 6985.5 87 4429.0 88 4429.0 89 4429.0 90 3154.5 91 1880.0 92 1880.0 93 1880.0 94 1040.5 95 201.0 96 201.0 97 201.0 98 118.5 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39167483321472873 2 0.6726120733927076 3 0.6865091820752856 4 0.6954145132138342 5 0.7059172131664722 6 0.7064363580310513 7 0.724526559850614 8 0.7129057017281134 9 0.7002864880968069 10 0.6940567497218582 11 0.7076743188619705 12 0.70927168767606 13 0.710270043184866 14 0.7189357690013011 15 0.7145829389829074 16 0.7082733321672541 17 0.7147027416439641 18 0.7184565583570742 19 0.7212120195613786 20 0.7147027416439641 21 0.7125462937449433 22 0.7210922169003218 23 0.710789188049445 24 0.7145829389829074 25 0.7172185975261549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2504118.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.34815915217965 #Duplication Level Percentage of deduplicated Percentage of total 1 88.5166196449612 58.72914767816836 2 6.798045802814448 9.020756496978795 3 1.6554441509988262 3.2950701599404506 4 0.7700848655958713 2.0437485289295894 5 0.46330658138573655 1.536976939901656 6 0.31604298369219197 1.2581322108563562 7 0.2192894849746982 1.0184617554650548 8 0.17900302187955877 0.9501216787508848 9 0.13117181336971645 0.7832707514760556 >10 0.844021477047789 10.47823459548122 >50 0.06256772238417138 2.8633638091645914 >100 0.0405313788236061 5.187218289343039 >500 0.003024275056430019 1.3374865196698285 >1k 7.258260135390349E-4 0.9191608031199864 >5k 1.2097100225650582E-4 0.5788497827542213 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 8110 0.3238665270566323 No Hit GTATCAACGCAGAGTACTTTTTTTT 6314 0.25214466730401686 No Hit GNNNNNNNNNNNNNNNNNNNNNNNN 3675 0.14675825979446655 No Hit TATCAACGCAGAGTACTTTTTTTTT 3668 0.1464787202520009 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.9934220352235794E-5 2 0.0 0.0 0.0 0.0 3.9934220352235794E-5 3 0.0 0.0 0.0 0.0 3.9934220352235794E-5 4 0.0 0.0 0.0 0.0 3.9934220352235794E-5 5 0.0 3.9934220352235794E-5 0.0 0.0 3.9934220352235794E-5 6 0.0 3.9934220352235794E-5 0.0 0.0 7.986844070447159E-5 7 0.0 3.9934220352235794E-5 0.0 0.0 7.986844070447159E-5 8 0.0 3.9934220352235794E-5 0.0 0.0 7.986844070447159E-5 9 0.0 3.9934220352235794E-5 0.0 0.0 1.198026610567074E-4 10 0.0 3.9934220352235794E-5 0.0 0.0 1.198026610567074E-4 11 0.0 3.9934220352235794E-5 0.0 0.0 1.198026610567074E-4 12 0.0 3.9934220352235794E-5 0.0 3.9934220352235794E-5 1.5973688140894318E-4 13 0.0 3.9934220352235794E-5 0.0 3.9934220352235794E-5 1.5973688140894318E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1215 0.0 14.783583 1 GTATCAA 3780 0.0 13.425908 1 CTATACG 55 0.0030700173 12.09055 4 CTCGACG 70 0.001491816 10.857257 13 TAGATCG 70 0.0014923167 10.856821 5 CGAACGT 150 2.1354936E-9 10.766348 4 TACCGTC 170 1.5625119E-9 10.058323 7 ATTAGAC 105 4.10824E-5 9.952086 3 AACCGCG 115 1.02754E-5 9.91255 7 GTCTTAA 280 0.0 9.503732 1 TCGAACG 190 1.0531949E-9 9.49972 3 CGAACCT 270 0.0 9.499719 16 TTAGTAC 90 0.0011143102 9.499719 3 TAAGGCG 80 0.0045181606 9.499719 5 ATCGATC 80 0.0045181606 9.499719 8 TATCAAC 5285 0.0 9.365095 2 CGGTTCT 265 0.0 9.320853 12 TCGCCAG 205 4.2382453E-10 9.268018 17 ATTCGAA 185 6.6556822E-9 9.246875 1 CGTCGTA 165 1.06634616E-7 9.212033 10 >>END_MODULE