FastQCFastQC Report
Thu 2 Feb 2017
SRR4050244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504118
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113410.4528939930147062No Hit
TATCAACGCAGAGTACTTTTTTTTT78940.31524073546054937No Hit
GGTATCAACGCAGAGTACTTTTTTT41580.16604648822459644No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38360.15318766927117652No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36120.1442424039122757No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29790.11896404242931044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.015.8327951
GTATCAA43800.013.1995571
CGATGCA600.005828350311.0958074
TATAACG700.001477524410.8697982
CGAACCT1803.6379788E-1110.54844416
ACTAGAC1303.785044E-710.2422853
CGAACGT750.002623934510.1447394
CTAGAAC2350.010.1177583
CGTATAG856.344287E-410.1014671
CCGGTAG856.63571E-410.0522489
TAGAACC2002.5465852E-119.9862274
AGAACCG1651.00408215E-89.7988965
ATCACGA2151.0913936E-119.71496312
CAAGACG1901.031367E-99.5106944
GAGCGCT1201.7135624E-59.49416811
CGCGGTC1304.276042E-69.4937910
GCTCGTA1401.0685908E-69.493799
AACCGCG1106.8927846E-59.4932217
GTTATTC2357.2759576E-129.3083373
GTATTAC1554.0091982E-79.2325231
Produced by FastQC (version 0.11.5)