##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050244_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2504118
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.615850371268447	31.0	31.0	34.0	27.0	34.0
2	30.73749599659441	31.0	31.0	34.0	27.0	34.0
3	30.849615313655345	31.0	31.0	34.0	27.0	34.0
4	34.50346149822013	37.0	35.0	37.0	31.0	37.0
5	34.42307990278413	37.0	35.0	37.0	31.0	37.0
6	34.45910096888405	37.0	35.0	37.0	31.0	37.0
7	34.36552311033266	37.0	35.0	37.0	30.0	37.0
8	34.343068896913	37.0	35.0	37.0	30.0	37.0
9	35.94353061636872	39.0	37.0	39.0	31.0	39.0
10	35.83079671165656	39.0	37.0	39.0	30.0	39.0
11	35.82417761463318	39.0	37.0	39.0	30.0	39.0
12	35.73082139100474	39.0	37.0	39.0	30.0	39.0
13	35.72847924898108	39.0	37.0	39.0	30.0	39.0
14	36.901669170542284	40.0	37.0	41.0	31.0	41.0
15	36.87600105106868	40.0	37.0	41.0	31.0	41.0
16	36.84400695174908	40.0	37.0	41.0	31.0	41.0
17	36.8390095834142	40.0	37.0	41.0	31.0	41.0
18	36.81707571288573	40.0	37.0	41.0	31.0	41.0
19	36.852053697150055	40.0	37.0	41.0	31.0	41.0
20	36.826456660588676	40.0	37.0	41.0	30.0	41.0
21	36.757555354819544	40.0	37.0	41.0	30.0	41.0
22	36.691898704454026	40.0	37.0	41.0	30.0	41.0
23	36.605736231279835	40.0	37.0	41.0	30.0	41.0
24	36.540940962047316	40.0	37.0	41.0	30.0	41.0
25	36.26061631280954	40.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9689807445754717
1101	2	1.0182150611392728
1101	3	1.01991038330711
1101	4	0.8974866760646378
1101	5	0.9913158938627902
1101	6	0.9704040572249397
1101	7	1.0596958206900524
1101	8	1.0112096672640263
1101	9	1.1121831551880277
1101	10	1.1280806047464722
1101	11	1.1768915460532696
1101	12	1.1754291720328496
1101	13	1.2326926512796845
1101	14	1.398680226843524
1101	15	1.4227052518604282
1101	16	1.4371794008813978
1101	17	1.3406807225337403
1101	18	1.332803414837457
1101	19	1.3909997452433984
1101	20	1.4425354166286937
1101	21	1.3913092068204165
1101	22	1.4618890142666814
1101	23	1.5397853414804032
1101	24	1.6456071259808525
1101	25	1.4675479208896292
1102	1	0.972940754764064
1102	2	1.105062901450843
1102	3	1.0272899294193643
1102	4	0.9277273918751661
1102	5	1.0104894372099267
1102	6	0.9181465698688243
1102	7	1.063160932812039
1102	8	1.0548895819903024
1102	9	1.2644886544269838
1102	10	1.3204552609124462
1102	11	1.2603788848284978
1102	12	1.258190841954658
1102	13	1.286038105239676
1102	14	1.4486981229322708
1102	15	1.4645074602419115
1102	16	1.5149267807660252
1102	17	1.5307792108818745
1102	18	1.4483356098391909
1102	19	1.5608290450418991
1102	20	1.5723907576100729
1102	21	1.5662288395522168
1102	22	1.5852302615105387
1102	23	1.6240436776106506
1102	24	1.6553520107337576
1102	25	1.7293499889096253
1103	1	0.8851669730288698
1103	2	0.9150606811391526
1103	3	0.8172684301140407
1103	4	0.8666045829747873
1103	5	0.8414106410310396
1103	6	0.8024474885956963
1103	7	0.9126701506051731
1103	8	0.8945786450304354
1103	9	1.0365145935779978
1103	10	1.0844140416619155
1103	11	0.9854133074366374
1103	12	1.1344130924330358
1103	13	1.2241672340570275
1103	14	1.2915714473454685
1103	15	1.3351515567208665
1103	16	1.3377161999503784
1103	17	1.2692565903385926
1103	18	1.2093250056149998
1103	19	1.2607336610737931
1103	20	1.2909631010810187
1103	21	1.3198229253573501
1103	22	1.3402616783377042
1103	23	1.3438445417046267
1103	24	1.385221191213212
1103	25	1.3426339854395621
1104	1	0.8309484391422828
1104	2	0.8919100542708165
1104	3	0.8633711383637177
1104	4	0.7906430874141748
1104	5	0.8374553472283353
1104	6	0.8228920966356696
1104	7	0.9238768163795044
1104	8	0.8863624312259475
1104	9	1.0169377854755552
1104	10	1.0589187933685125
1104	11	1.032458549037493
1104	12	1.0772007603043647
1104	13	1.0678461257716592
1104	14	1.2678207223198115
1104	15	1.2798934475877743
1104	16	1.2408516570511665
1104	17	1.188315841665947
1104	18	1.229561029351224
1104	19	1.2536696803483238
1104	20	1.2921588356993183
1104	21	1.2145257763813078
1104	22	1.332178199999234
1104	23	1.337503355785742
1104	24	1.2735795100460336
1104	25	1.2423205512718951
1105	1	0.7008710016202855
1105	2	0.6453070294385803
1105	3	0.6677306891806722
1105	4	0.5733482343978551
1105	5	0.643692360547135
1105	6	0.6312912859261317
1105	7	0.6691144655489225
1105	8	0.7323781458198511
1105	9	0.8367487902330808
1105	10	0.8386763185470656
1105	11	0.8472588238157996
1105	12	0.7954615953124815
1105	13	0.917341135479731
1105	14	1.0108226752895746
1105	15	1.0072441078505179
1105	16	1.007515201237105
1105	17	1.0373180671431186
1105	18	0.9687158022049402
1105	19	0.9391464547689594
1105	20	1.0869039844997204
1105	21	1.1162099289400373
1105	22	1.1324998155139951
1105	23	1.0748297742435824
1105	24	1.1094502847618841
1105	25	1.101341398088664
1106	1	0.6173281816018807
1106	2	0.6627349892445125
1106	3	0.6422384958564464
1106	4	0.6341649203613926
1106	5	0.5973134184352702
1106	6	0.6471406351120095
1106	7	0.6925081416336809
1106	8	0.7238425330377609
1106	9	0.8660198203737011
1106	10	0.8023792938968057
1106	11	0.8634285630603173
1106	12	0.9316776087101388
1106	13	0.8845336315215704
1106	14	0.9758280180898282
1106	15	1.0237929627828848
1106	16	0.9025387585646811
1106	17	0.9941226975152446
1106	18	1.0596781742713617
1106	19	0.9757421724796487
1106	20	1.0541109908368824
1106	21	0.9433702039128704
1106	22	1.0138444763686891
1106	23	1.002013142488316
1106	24	0.9976455845760697
1106	25	1.0044185799921905
1107	1	0.4702369155537305
1107	2	0.453773978295132
1107	3	0.46891047756820115
1107	4	0.43581789136264604
1107	5	0.3292403100970489
1107	6	0.352513756691053
1107	7	0.4819598357809127
1107	8	0.4317519978751463
1107	9	0.49293072512578107
1107	10	0.5220222287401199
1107	11	0.4845826831259288
1107	12	0.6310872791111706
1107	13	0.5244413989132184
1107	14	0.604096491764885
1107	15	0.5683906492277089
1107	16	0.5980515090249838
1107	17	0.5843027105796139
1107	18	0.5899935485405692
1107	19	0.6754247469185231
1107	20	0.7244285427239063
1107	21	0.7154324367277525
1107	22	0.6833235317691475
1107	23	0.7444790894716817
1107	24	0.7549849687192847
1107	25	0.586160624786018
1108	1	0.4804369842433083
1108	2	0.5371022282336568
1108	3	0.46614752990009833
1108	4	0.45454420249765803
1108	5	0.4846214153114232
1108	6	0.3957658295883135
1108	7	0.47320521651148795
1108	8	0.527065160167119
1108	9	0.6389859094137194
1108	10	0.6373522633261643
1108	11	0.6532435116362478
1108	12	0.5982774471783401
1108	13	0.6661880219862653
1108	14	0.6466438851863643
1108	15	0.6522083434560457
1108	16	0.7635914927202023
1108	17	0.6332128143874414
1108	18	0.7558950253533894
1108	19	0.7467276788702719
1108	20	0.6928253223818572
1108	21	0.7127812256735027
1108	22	0.7851099641982131
1108	23	0.7738549002404582
1108	24	0.594144264790387
1108	25	0.6772294956649816
1109	1	0.32502195752188
1109	2	0.40849545061990256
1109	3	0.35962020062928346
1109	4	0.3321222071941179
1109	5	0.3148621989412632
1109	6	0.3582071754053615
1109	7	0.32639619622446503
1109	8	0.4107560296255457
1109	9	0.47828437212221786
1109	10	0.4943964127733622
1109	11	0.5102592492125382
1109	12	0.5261379946129381
1109	13	0.43640701599421305
1109	14	0.5292798370985068
1109	15	0.5330817446620415
1109	16	0.5811054705045251
1109	17	0.5348462242615923
1109	18	0.4909138675607707
1109	19	0.5373343465148608
1109	20	0.5709785406255463
1109	21	0.651953883408062
1109	22	0.5387610957231672
1109	23	0.5253440659632886
1109	24	0.6787495525566598
1109	25	0.5711965202641451
1110	1	0.1193640570835548
1110	2	0.1548702703654996
1110	3	0.14810919122155752
1110	4	0.12585137520731848
1110	5	0.02817139506894506
1110	6	0.19004461201524236
1110	7	0.17351952110215052
1110	8	0.1848203104585764
1110	9	0.20467966891350642
1110	10	0.2040459936100092
1110	11	0.17846012082443963
1110	12	0.17335873009690772
1110	13	0.27502924415531993
1110	14	0.2477708102774301
1110	15	0.2664551896689886
1110	16	0.2433360692965323
1110	17	0.16071354539971594
1110	18	0.10061881073751522
1110	19	0.12127915895224106
1110	20	0.18117350224107298
1110	21	0.12242816945295232
1110	22	0.23404430665174658
1110	23	0.16157693831496545
1110	24	0.2722083601433596
1110	25	0.2204682888355194
1111	1	-0.24010060171171688
1111	2	-0.19438570549864664
1111	3	-0.16261732961771358
1111	4	-0.13192843103731633
1111	5	-0.2643556250187018
1111	6	-0.23088694377817376
1111	7	-0.13992619944789908
1111	8	-0.20757264356129213
1111	9	-0.25631156918191067
1111	10	-0.20151161190918288
1111	11	-0.16429266591529057
1111	12	-0.24859740606843417
1111	13	-0.10045137389823111
1111	14	-0.11985517721129213
1111	15	-0.24706983067460442
1111	16	-0.24895510266910748
1111	17	-0.20777682615548088
1111	18	-0.3455313381302503
1111	19	-0.37911261978968014
1111	20	-0.2938095923576114
1111	21	-0.2893695163077439
1111	22	-0.19889177403171487
1111	23	-0.2623792915556038
1111	24	-0.17842687735625873
1111	25	-0.26247602019218874
1112	1	-0.4805049573822018
1112	2	-0.5599512819163124
1112	3	-0.31228752748758026
1112	4	-0.36818201578105914
1112	5	-0.4138517923527729
1112	6	-0.4045540125219489
1112	7	-0.4350578521217443
1112	8	-0.48831779096230576
1112	9	-0.5357593046737605
1112	10	-0.5040978197895143
1112	11	-0.47317387210096484
1112	12	-0.5195861733958864
1112	13	-0.4201258968548558
1112	14	-0.5140105471089171
1112	15	-0.5049610598779566
1112	16	-0.5368568992348273
1112	17	-0.39625965647399397
1112	18	-0.5046488264302624
1112	19	-0.4248185732316614
1112	20	-0.5078156839969594
1112	21	-0.3992399577062429
1112	22	-0.4248989543604438
1112	23	-0.3497832466705546
1112	24	-0.5100863412509185
1112	25	-0.48064612049083166
1113	1	-0.5542091450837674
1113	2	-0.5261079603632552
1113	3	-0.4761223283890921
1113	4	-0.6679544305028031
1113	5	-0.61297886705416
1113	6	-0.6406478014967547
1113	7	-0.6084009054729478
1113	8	-0.646699705989775
1113	9	-0.7585187154684974
1113	10	-0.661552506920458
1113	11	-0.6926366074950039
1113	12	-0.6937890178807535
1113	13	-0.7519530689035818
1113	14	-0.8935738968286842
1113	15	-0.8463316927681106
1113	16	-0.8526517811045977
1113	17	-0.8338432350151948
1113	18	-0.8461016858152277
1113	19	-0.9427590699459145
1113	20	-0.9216829340118551
1113	21	-0.9150850584419814
1113	22	-0.8975716134438798
1113	23	-0.780610566616609
1113	24	-0.8246497020077967
1113	25	-0.8729862534218285
1114	1	-0.9105219391589294
1114	2	-1.4220494447045695
1114	3	-1.023080935040074
1114	4	-0.7891722134845693
1114	5	-1.0026047480952869
1114	6	-0.9440902047926514
1114	7	-0.9211016168440764
1114	8	-1.0524497075689254
1114	9	-1.0691299618324308
1114	10	-1.0708362587064215
1114	11	-1.0553084453520754
1114	12	-1.1510609268162
1114	13	-1.0271963937804287
1114	14	-1.2848680253488993
1114	15	-1.3570056951136777
1114	16	-1.2395101045169952
1114	17	-1.2966148529991557
1114	18	-1.2025339412151155
1114	19	-1.2285732260242384
1114	20	-1.2199791916697649
1114	21	-1.2734557345210433
1114	22	-1.2631156761107647
1114	23	-1.451182720979702
1114	24	-1.5434296539151973
1114	25	-1.6336994574776185
1115	1	-0.8207816375760508
1115	2	-1.2520720515186667
1115	3	-1.1660484993075606
1115	4	-0.9613626247935869
1115	5	-1.1456633363880826
1115	6	-1.0914895606621684
1115	7	-1.2150292023985614
1115	8	-1.2035422137939307
1115	9	-1.6062596619605145
1115	10	-1.3719719524508704
1115	11	-1.5459912507069475
1115	12	-1.4685630644000511
1115	13	-1.8661699199260013
1115	14	-1.6764904288592462
1115	15	-1.7920268340326544
1115	16	-1.7836511131928248
1115	17	-1.7218705500470648
1115	18	-1.700266442782194
1115	19	-1.7354052803834037
1115	20	-1.904225452951522
1115	21	-1.9039162959733247
1115	22	-2.0036027714296267
1115	23	-1.860297494732194
1115	24	-1.8041018437118552
1115	25	-1.7025017187624982
1116	1	-1.5450741776966588
1116	2	-1.545984621823333
1116	3	-1.5691858810925794
1116	4	-1.554031083049992
1116	5	-1.8409938204571716
1116	6	-1.7491193514291012
1116	7	-1.89679603172916
1116	8	-2.0159640879866814
1116	9	-2.1274763312394143
1116	10	-2.023387220784109
1116	11	-2.215726845773972
1116	12	-2.4316316624846124
1116	13	-2.2931280711966124
1116	14	-2.6210021675399418
1116	15	-2.541991820972939
1116	16	-2.607317065821313
1116	17	-2.7240409862767976
1116	18	-2.737033392246495
1116	19	-2.5815641827821807
1116	20	-2.600342781621258
1116	21	-2.6090437142553355
1116	22	-2.7241790098306495
1116	23	-2.930306621158529
1116	24	-2.8256013562678177
1116	25	-3.08582189548477
1201	1	0.9378492933509435
1201	2	1.0672444309833367
1201	3	0.9978168934823124
1201	4	0.9342335038060625
1201	5	1.0139370540356865
1201	6	1.033227355731178
1201	7	1.0040449490403631
1201	8	1.0389142209153732
1201	9	1.2339704862796097
1201	10	1.1013079304598676
1201	11	1.1770763998002138
1201	12	1.1878370358431596
1201	13	1.28471612753642
1201	14	1.4457844455425857
1201	15	1.4392595560310966
1201	16	1.4970041111595123
1201	17	1.4658346580962274
1201	18	1.4326876064173106
1201	19	1.4264870982789617
1201	20	1.4495233279885298
1201	21	1.4657573198704057
1201	22	1.469893029296827
1201	23	1.4494419202569802
1201	24	1.4492295321830113
1201	25	1.5875178335322815
1202	1	0.8384680799952591
1202	2	0.9377963364474198
1202	3	0.7979680030681067
1202	4	0.8642781838987332
1202	5	0.9436643910717848
1202	6	0.9529932877583676
1202	7	1.0041988539596005
1202	8	1.0223531629156
1202	9	1.0906434804178105
1202	10	1.1986141338703078
1202	11	1.149625740667048
1202	12	1.0766377828596632
1202	13	1.1698201672618254
1202	14	1.2877394435826233
1202	15	1.322641718830667
1202	16	1.2706143376202945
1202	17	1.3886063814726128
1202	18	1.3661093078349822
1202	19	1.3472714688425853
1202	20	1.3330697368252729
1202	21	1.3724843948237933
1202	22	1.3144335491900705
1202	23	1.3533364175865472
1202	24	1.3928628451895833
1202	25	1.408609787722419
1203	1	0.699266633472039
1203	2	0.8392656121941542
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2211	8	-0.36433374137524766
2211	9	-0.2860265686266672
2211	10	-0.44117598068549313
2211	11	-0.3672319332442129
2211	12	-0.39640049869819904
2211	13	-0.40569950919512365
2211	14	-0.4766174555375571
2211	15	-0.46944177735039716
2211	16	-0.4888033188773875
2211	17	-0.4301228478550314
2211	18	-0.41306248701813786
2211	19	-0.36686682995084396
2211	20	-0.5154944114264524
2211	21	-0.4177084492623422
2211	22	-0.40128285542323283
2211	23	-0.4882784817067929
2211	24	-0.45289384856037884
2211	25	-0.3425530057979813
2212	1	-0.5318160178393789
2212	2	-0.47889882390077076
2212	3	-0.5149369435933018
2212	4	-0.4537853063556412
2212	5	-0.530434456675394
2212	6	-0.5673792987872304
2212	7	-0.6492835488175785
2212	8	-0.5792691565017378
2212	9	-0.657406285379956
2212	10	-0.6839277874586358
2212	11	-0.7321246895083888
2212	12	-0.8676875421661734
2212	13	-0.8479503921166298
2212	14	-0.8518507064682694
2212	15	-0.8361112235376922
2212	16	-0.868191444697473
2212	17	-0.8141285370592044
2212	18	-0.8743191896457034
2212	19	-0.9303753171492914
2212	20	-0.9576402149095955
2212	21	-0.934816398725232
2212	22	-0.9373461186685859
2212	23	-0.9794579102712504
2212	24	-1.03423211317007
2212	25	-0.850517688259778
2213	1	-0.949119314737807
2213	2	-0.9046287415602627
2213	3	-0.8055916884928429
2213	4	-0.9171463850662107
2213	5	-0.7755405128215287
2213	6	-0.7770097415631838
2213	7	-0.9196844683519885
2213	8	-0.922890192140251
2213	9	-1.0648745799887678
2213	10	-1.004424286256274
2213	11	-0.9985978752552995
2213	12	-1.081517405410544
2213	13	-1.1193808319368088
2213	14	-1.202874898583758
2213	15	-1.2082076901996288
2213	16	-1.285786151952017
2213	17	-1.308349389106418
2213	18	-1.3374887542480707
2213	19	-1.2236981474866795
2213	20	-1.300001435519512
2213	21	-1.2364691204757392
2213	22	-1.2668321130257212
2213	23	-1.2734697358090656
2213	24	-1.2965564932882927
2213	25	-1.3016992030758132
2214	1	-1.067568677122111
2214	2	-1.6485151934277624
2214	3	-1.2482471590170654
2214	4	-1.10289804810634
2214	5	-1.2839810755309315
2214	6	-1.281143978047929
2214	7	-1.2082956286484645
2214	8	-1.3535041906590592
2214	9	-1.5458294414707936
2214	10	-1.466974549938918
2214	11	-1.369581688305324
2214	12	-1.4590988493827268
2214	13	-1.537005942072561
2214	14	-1.737034907702217
2214	15	-1.8713642556217494
2214	16	-1.7309006545477956
2214	17	-1.7014549830799979
2214	18	-1.737030068257539
2214	19	-1.8331857562983487
2214	20	-1.9697273883413118
2214	21	-1.9113919932882766
2214	22	-1.9122997882862904
2214	23	-1.813271290668105
2214	24	-1.8348148891926783
2214	25	-1.7437415374874021
2215	1	-1.224704282830455
2215	2	-1.3073704101965191
2215	3	-1.4683191760844316
2215	4	-1.292707718084415
2215	5	-1.477265873256414
2215	6	-1.3688320058033199
2215	7	-1.2713743761751957
2215	8	-1.3686476962763905
2215	9	-1.638497836902033
2215	10	-1.5317227972465801
2215	11	-1.6008195722536414
2215	12	-1.6558497145573199
2215	13	-1.8173245804596192
2215	14	-1.8821469382906812
2215	15	-2.0519798480819205
2215	16	-1.9095157667003022
2215	17	-1.956895832027712
2215	18	-1.9458555706035412
2215	19	-1.9202237915424618
2215	20	-1.875988199877824
2215	21	-1.8290004369493857
2215	22	-1.7071535500382495
2215	23	-1.778551276815918
2215	24	-1.989064174687492
2215	25	-2.0577852347468166
2216	1	-1.7999293178002986
2216	2	-1.7428684685811682
2216	3	-1.836756177151205
2216	4	-1.7228946276467951
2216	5	-1.7587480868340393
2216	6	-1.815193461118625
2216	7	-1.745831812559139
2216	8	-1.858964628505774
2216	9	-2.179345076761564
2216	10	-2.2440295545542526
2216	11	-2.184359556841777
2216	12	-2.2664235264263084
2216	13	-2.1165997060854522
2216	14	-2.5156195474047323
2216	15	-2.526815964830959
2216	16	-2.4455952175257707
2216	17	-2.500291076002398
2216	18	-2.6212215866081507
2216	19	-2.5611708584094615
2216	20	-2.712614055889503
2216	21	-2.6424472684470075
2216	22	-2.7470198994854513
2216	23	-2.661055199269576
2216	24	-2.6687842234670427
2216	25	-2.722824882220152
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	38906.0
3	1.0
4	760.0
5	5565.0
6	6639.0
7	5193.0
8	4407.0
9	3677.0
10	3308.0
11	3093.0
12	2887.0
13	2640.0
14	2616.0
15	2745.0
16	2735.0
17	2939.0
18	3348.0
19	4177.0
20	5582.0
21	7476.0
22	9365.0
23	11310.0
24	13872.0
25	15486.0
26	17436.0
27	20120.0
28	23030.0
29	28239.0
30	36242.0
31	47400.0
32	61399.0
33	81548.0
34	110169.0
35	151584.0
36	221903.0
37	392980.0
38	1153341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11306277111835	18.850546407762007	12.905394132292924	26.130996688826713
2	16.19144606918145	21.358269485362392	37.4854582377361	24.964826207720066
3	18.46166993727931	25.283832471153517	29.229373376174767	27.025124215392403
4	12.946610601681549	17.30511873207617	37.336236955366196	32.41203371087608
5	14.498131450077137	36.50754159788518	34.12376548308506	14.870561468952632
6	31.43683556592504	37.961463323039304	16.849508803189682	13.752192307845965
7	27.668104469034798	31.45895873609704	22.371611144013325	18.501325650854834
8	25.785818428830375	34.405211594223736	20.281625120951205	19.527344855994688
9	26.5881665131859	15.165763220045095	19.47690879888056	38.76916146788844
10	15.13827488848155	27.783089130894904	32.7754815160918	24.303154464531744
11	35.18961571284749	22.747935278709917	23.18402166694421	18.878427341498373
12	23.70123127843378	24.727642743955425	29.87862930020958	21.692496677401216
13	28.96350996945286	21.19190143446543	25.326088389012934	24.518500207068772
14	22.674521700789818	20.37053012294558	26.79669727526008	30.158250901004525
15	24.11856651195705	27.83111811843712	23.571691574356063	24.478623795249767
16	23.990337462022342	27.158057333082013	24.7814387900822	24.070166414813443
17	23.5990501385015	26.2244283418479	26.05253951366325	24.123982005987354
18	24.195711927875344	25.690255496066666	26.96060754543832	23.153425030619672
19	25.18878047553232	25.52530870384917	26.49535821067777	22.790552609940743
20	24.501442478187112	25.580585489595947	26.56024862381511	23.35772340840184
21	24.52648444351439	25.38913178564525	26.445985600845994	23.638398169994364
22	23.95140424434135	25.375556040984197	26.811528513186644	23.861511201487808
23	23.608781564688442	25.127113364514646	26.77838905075909	24.485716020037827
24	23.444112404969065	25.844181413093093	26.83613326075766	23.875572921180183
25	23.811009081247995	25.397421914406472	26.794174486641314	23.997394517704222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	474.0
1	474.0
2	952.0
3	1430.0
4	1430.0
5	1430.0
6	3898.0
7	6366.0
8	6366.0
9	6366.0
10	7998.0
11	9630.0
12	9630.0
13	9630.0
14	12293.5
15	14957.0
16	14957.0
17	14957.0
18	24484.5
19	34012.0
20	34012.0
21	34012.0
22	48436.5
23	62861.0
24	62861.0
25	62861.0
26	84276.0
27	105691.0
28	105691.0
29	105691.0
30	125566.5
31	145442.0
32	145442.0
33	145442.0
34	166143.0
35	186844.0
36	186844.0
37	186844.0
38	207653.0
39	228462.0
40	228462.0
41	228462.0
42	248188.0
43	267914.0
44	267914.0
45	267914.0
46	282667.5
47	297421.0
48	297421.0
49	297421.0
50	300041.0
51	302661.0
52	302661.0
53	302661.0
54	282989.5
55	263318.0
56	263318.0
57	263318.0
58	236452.5
59	209587.0
60	209587.0
61	209587.0
62	180547.0
63	151507.0
64	151507.0
65	151507.0
66	124331.5
67	97156.0
68	97156.0
69	97156.0
70	75565.0
71	53974.0
72	53974.0
73	53974.0
74	42075.5
75	30177.0
76	30177.0
77	30177.0
78	24310.0
79	18443.0
80	18443.0
81	18443.0
82	13898.5
83	9354.0
84	9354.0
85	9354.0
86	6836.5
87	4319.0
88	4319.0
89	4319.0
90	3119.5
91	1920.0
92	1920.0
93	1920.0
94	1047.0
95	174.0
96	174.0
97	174.0
98	99.0
99	24.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.42897339502371695
2	0.004911909103325003
3	0.0
4	0.00475217222191606
5	0.16177352664690722
6	0.04185106292914312
7	0.0025957243228953266
8	3.5940798317012215E-4
9	1.597368814089432E-4
10	0.0019168425769073183
11	0.0010782239495103665
12	0.002156447899020733
13	0.00475217222191606
14	0.009823818206650006
15	0.002875263865360977
16	5.590790849313012E-4
17	0.006948554341289029
18	3.5940798317012215E-4
19	7.587501866924801E-4
20	0.0037538167131101648
21	0.0032746060688833355
22	0.0012379608309193097
23	0.002156447899020733
24	0.0025957243228953266
25	0.006669014798823378
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2504118.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.72550660688391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.3195617131758	58.048479368964266
2	6.9151078986514305	9.089979397602594
3	1.7063040305329003	3.3644309049642835
4	0.7545846094375195	1.983818229321544
5	0.47667880481119945	1.5664977967490008
6	0.30770772217802406	1.2134547556200552
7	0.23557319397378623	1.0838217261850183
8	0.17293970465695524	0.9093239760818611
9	0.13383669598530018	0.7916836181602823
>10	0.8677090171127829	10.666779703584648
>50	0.0643453297247801	2.938206403794587
>100	0.04193879038399823	5.415541180944138
>500	0.0030430240783042824	1.3224684269279354
>1k	5.477443340929799E-4	0.8360979722203405
>5k	6.086048156588666E-5	0.3157667875182934
>10k+	6.086048156588666E-5	0.453649751361156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11341	0.4528939930147062	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7894	0.31524073546054937	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4158	0.16604648822459644	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3836	0.15318766927117652	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3612	0.1442424039122757	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2979	0.11896404242931044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9934220352235794E-5
2	0.0	0.0	0.0	0.0	3.9934220352235794E-5
3	0.0	0.0	0.0	0.0	3.9934220352235794E-5
4	0.0	0.0	0.0	0.0	3.9934220352235794E-5
5	0.0	0.0	0.0	0.0	3.9934220352235794E-5
6	0.0	0.0	0.0	0.0	3.9934220352235794E-5
7	0.0	0.0	0.0	0.0	3.9934220352235794E-5
8	0.0	0.0	0.0	0.0	3.9934220352235794E-5
9	0.0	0.0	0.0	3.9934220352235794E-5	3.9934220352235794E-5
10	0.0	0.0	0.0	3.9934220352235794E-5	3.9934220352235794E-5
11	0.0	0.0	0.0	3.9934220352235794E-5	3.9934220352235794E-5
12	0.0	0.0	0.0	7.986844070447159E-5	1.99671101761179E-4
13	0.0	0.0	0.0	7.986844070447159E-5	1.99671101761179E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1410	0.0	15.832795	1
GTATCAA	4380	0.0	13.199557	1
CGATGCA	60	0.0058283503	11.095807	4
TATAACG	70	0.0014775244	10.869798	2
CGAACCT	180	3.6379788E-11	10.548444	16
ACTAGAC	130	3.785044E-7	10.242285	3
CGAACGT	75	0.0026239345	10.144739	4
CTAGAAC	235	0.0	10.117758	3
CGTATAG	85	6.344287E-4	10.101467	1
CCGGTAG	85	6.63571E-4	10.052248	9
TAGAACC	200	2.5465852E-11	9.986227	4
AGAACCG	165	1.00408215E-8	9.798896	5
ATCACGA	215	1.0913936E-11	9.714963	12
CAAGACG	190	1.031367E-9	9.510694	4
GAGCGCT	120	1.7135624E-5	9.494168	11
CGCGGTC	130	4.276042E-6	9.49379	10
GCTCGTA	140	1.0685908E-6	9.49379	9
AACCGCG	110	6.8927846E-5	9.493221	7
GTTATTC	235	7.2759576E-12	9.308337	3
GTATTAC	155	4.0091982E-7	9.232523	1
>>END_MODULE