FastQCFastQC Report
Thu 2 Feb 2017
SRR4050243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521530
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN58110.2304553187945414No Hit
GTATCAACGCAGAGTACTTTTTTTT57660.22867068803464563No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN45080.1787803436802259No Hit
TATCAACGCAGAGTACTTTTTTTTT32850.13027804547239177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA804.3291948E-1016.62435316
GGTATCA9500.014.0016931
GTATCAA31950.013.5305871
CCGGTAG1051.9967047E-812.6661749
GCGTTAT701.090183E-412.2157621
ACGAACG1052.7243186E-711.76121115
TAAACGC1850.011.29708418
CTTATAC2300.011.1530763
AACCGCG1408.358256E-910.8571547
ACGCTTA700.001492438110.85672117
AAATTCG700.001492686910.856503514
AACGAAC1252.2435597E-710.63937414
CGGTAGC1355.695074E-810.55514510
ATAGCAC1553.6616257E-910.4201983
GTATTAG3500.010.3155341
AGGCCCG2151.8189894E-1210.16239510
CGTTATT856.588612E-410.060042
CCCCTAT1803.929017E-1010.0289911
CGCTTAC951.645047E-49.99981218
GTCTAGC1151.0254671E-59.9142421
Produced by FastQC (version 0.11.5)