##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050243_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2521530
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.75168409656042	31.0	30.0	34.0	26.0	34.0
2	29.859015359722072	31.0	30.0	34.0	25.0	34.0
3	29.97699610950494	31.0	31.0	34.0	26.0	34.0
4	33.35688847644089	37.0	35.0	37.0	27.0	37.0
5	33.43998722997545	37.0	35.0	37.0	28.0	37.0
6	33.497341693336985	37.0	35.0	37.0	28.0	37.0
7	33.38836658695316	37.0	35.0	37.0	28.0	37.0
8	33.33793173192466	37.0	35.0	37.0	27.0	37.0
9	34.79762168207398	39.0	35.0	39.0	27.0	39.0
10	34.6975209495822	39.0	35.0	39.0	27.0	39.0
11	34.71914908805368	39.0	35.0	39.0	27.0	39.0
12	34.61517531022832	39.0	35.0	39.0	27.0	39.0
13	34.60274515869333	39.0	35.0	39.0	27.0	39.0
14	35.46289316407102	40.0	36.0	41.0	25.0	41.0
15	35.585289090353875	40.0	36.0	41.0	27.0	41.0
16	35.59560703223836	40.0	36.0	41.0	27.0	41.0
17	35.58126375652878	40.0	36.0	41.0	27.0	41.0
18	35.41964957783568	40.0	36.0	41.0	25.0	41.0
19	35.54815766617887	40.0	36.0	41.0	26.0	41.0
20	35.50667689854969	40.0	36.0	41.0	26.0	41.0
21	35.471205577566	40.0	36.0	41.0	26.0	41.0
22	35.3933258775426	40.0	36.0	41.0	25.0	41.0
23	35.28731484455866	40.0	36.0	41.0	25.0	41.0
24	35.22786046566965	40.0	36.0	41.0	25.0	41.0
25	34.94384401533989	39.0	36.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1817475220515021
1101	2	1.18693594487495
1101	3	1.109792799628952
1101	4	1.163219833874443
1101	5	1.102575786123822
1101	6	1.1397401991011762
1101	7	1.1391610085251571
1101	8	1.1606961566259741
1101	9	1.3808975338231875
1101	10	1.4365123837706122
1101	11	1.2858529238099976
1101	12	1.3687476076853713
1101	13	1.2926082897851217
1101	14	1.6670565114198652
1101	15	1.5200854395128829
1101	16	1.5575735813093985
1101	17	1.548475558493827
1101	18	1.5563075094849026
1101	19	1.530861113456531
1101	20	1.533293166697888
1101	21	1.5372403187109853
1101	22	1.5776997844087717
1101	23	1.527199565617103
1101	24	1.535586151714888
1101	25	1.6265615928333332
1102	1	0.9674718285184447
1102	2	0.9629357035148871
1102	3	0.869149776191886
1102	4	0.9919927190825959
1102	5	1.061246395004865
1102	6	1.0014793492120475
1102	7	0.9887957000116856
1102	8	0.9585626342272633
1102	9	1.103769919717699
1102	10	1.1008765759937873
1102	11	1.1408792471419744
1102	12	1.1626432094010397
1102	13	1.2290971545380032
1102	14	1.6106828458141749
1102	15	1.5196002052176283
1102	16	1.352979443341951
1102	17	1.3278070916833542
1102	18	1.5374411978186586
1102	19	1.4249792189811572
1102	20	1.449664921536403
1102	21	1.372505035389473
1102	22	1.4918432327401163
1102	23	1.453252373187766
1102	24	1.5018824815334142
1102	25	1.5007483854095014
1103	1	0.8295885049802045
1103	2	0.8812002251041413
1103	3	0.7812175736853071
1103	4	0.9148088231827316
1103	5	0.8943886516208934
1103	6	0.8061528637594506
1103	7	0.7896394774246787
1103	8	0.8333318704897579
1103	9	0.9639000738432699
1103	10	0.8865123837706079
1103	11	0.9076380262968584
1103	12	0.9963068255954681
1103	13	0.9183422374488899
1103	14	1.2833155934684228
1103	15	1.1030495838805408
1103	16	1.091258382538058
1103	17	1.027727138622602
1103	18	1.221320209585322
1103	19	1.0876335158429669
1103	20	1.1241609084428248
1103	21	1.1646430004996375
1103	22	1.183131292471856
1103	23	1.1707963521963478
1103	24	1.1124365268108747
1103	25	1.1751734423223752
1104	1	0.7210111232406895
1104	2	0.7291120387762788
1104	3	0.6962363414231056
1104	4	0.8379153500153436
1104	5	0.7535085515262949
1104	6	0.7091463772766318
1104	7	0.7052380614522633
1104	8	0.7486422417417202
1104	9	0.8129435465020478
1104	10	0.8889308280319526
1104	11	0.9490736989007047
1104	12	0.8818702476644305
1104	13	0.9492742497504949
1104	14	1.3440564359313143
1104	15	1.2489378047939326
1104	16	1.2160477329064676
1104	17	1.1248675592776252
1104	18	1.2929054815734133
1104	19	1.280057080119498
1104	20	1.1373271461810646
1104	21	1.0878444680112764
1104	22	1.1478794792744296
1104	23	1.0341538275880566
1104	24	1.1177727015830996
1104	25	1.0764910833177623
1105	1	0.49854001071565435
1105	2	0.5276524180606224
1105	3	0.5481487255284989
1105	4	0.6452063179013834
1105	5	0.6570559197008876
1105	6	0.6742783374104135
1105	7	0.5708459945581268
1105	8	0.6428515903162051
1105	9	0.7691620607245611
1105	10	0.7209658494148741
1105	11	0.6435802936554538
1105	12	0.5754153822186865
1105	13	0.641888176466189
1105	14	0.9207674690123113
1105	15	0.8165355182505536
1105	16	0.8354894467510832
1105	17	0.827917510238322
1105	18	0.8874963295277922
1105	19	0.7972884017946527
1105	20	0.7129907191039351
1105	21	0.7900206683220503
1105	22	0.791991659789602
1105	23	0.8232381510296634
1105	24	0.7803633518193394
1105	25	0.7136490114249199
1106	1	0.38187019019944657
1106	2	0.2750487069913241
1106	3	0.27483314896315036
1106	4	0.4429826754550916
1106	5	0.3516739052122162
1106	6	0.24973186692508875
1106	7	0.28230625069831206
1106	8	0.3430936631451331
1106	9	0.4446834277576741
1106	10	0.4294176901323823
1106	11	0.4416715601105139
1106	12	0.4926918129655036
1106	13	0.45627151557166457
1106	14	0.6446923336050574
1106	15	0.5281753652947998
1106	16	0.5774897966498642
1106	17	0.6109452772057367
1106	18	0.6765855572869057
1106	19	0.5795356802605198
1106	20	0.6516702439556781
1106	21	0.6363083493857431
1106	22	0.6018870114914279
1106	23	0.5610390014979245
1106	24	0.5476006867331691
1106	25	0.5342819711758438
1107	1	0.4912327686396907
1107	2	0.4842592999674018
1107	3	0.5061589962136495
1107	4	0.5412214240807174
1107	5	0.47617957591312177
1107	6	0.5330745339002902
1107	7	0.5568484116029992
1107	8	0.4579824878319556
1107	9	0.6071565735692417
1107	10	0.6324185130446693
1107	11	0.631731436906442
1107	12	0.5959926360147136
1107	13	0.5739902822164638
1107	14	0.8760903397200721
1107	15	0.7026860337159135
1107	16	0.622111222766442
1107	17	0.6265200903389214
1107	18	0.6381014685219171
1107	19	0.6810265070477541
1107	20	0.8271077498605166
1107	21	0.7103206150234698
1107	22	0.813099020260033
1107	23	0.7852684968103318
1107	24	0.8132311594079624
1107	25	0.715944993386266
1108	1	0.45603584346247317
1108	2	0.5125990015294946
1108	3	0.48010278111265237
1108	4	0.6954921433497319
1108	5	0.46728159011748005
1108	6	0.5184511183887608
1108	7	0.5570458877695899
1108	8	0.5761312896369404
1108	9	0.49723583591859466
1108	10	0.6513187619027292
1108	11	0.6168210997547945
1108	12	0.5997910477205437
1108	13	0.5898088652556908
1108	14	0.9206552006257951
1108	15	0.7366661852268308
1108	16	0.7357263474312319
1108	17	0.6255779576199672
1108	18	0.9178016015868309
1108	19	0.6861362603214118
1108	20	0.7610409720502957
1108	21	0.7711957463841941
1108	22	0.781456994442209
1108	23	0.7942831820233422
1108	24	0.746288971787088
1108	25	0.65634685705691
1109	1	0.2951989970483986
1109	2	0.30984318770396513
1109	3	0.32298765059188383
1109	4	0.37203670246993426
1109	5	0.38542898416780247
1109	6	0.3773166984891816
1109	7	0.398309596206019
1109	8	0.4705256258163075
1109	9	0.4452303408998617
1109	10	0.4566730398766694
1109	11	0.36658572142587786
1109	12	0.49834836218368395
1109	13	0.541720157759741
1109	14	0.7259301447786228
1109	15	0.5996950635054574
1109	16	0.5560081155722543
1109	17	0.5845463134060509
1109	18	0.5838753758984936
1109	19	0.5373852177854346
1109	20	0.506331678041974
1109	21	0.5713689500297647
1109	22	0.4371366306531357
1109	23	0.5324825320490234
1109	24	0.457827295524865
1109	25	0.40828452165859375
1110	1	-0.12954182121893254
1110	2	-0.01820791648451703
1110	3	-0.060341448168621525
1110	4	-0.12267203243931846
1110	5	-0.14889945735085774
1110	6	-0.19175216207229084
1110	7	-0.14775727833669094
1110	8	-0.04760514337844057
1110	9	-0.11395247608255943
1110	10	-0.2539607117963172
1110	11	-0.2064075531195897
1110	12	-0.23490084238610365
1110	13	-0.23219049530010238
1110	14	-0.0705469822440179
1110	15	-0.18545807236616696
1110	16	-0.17768966210256565
1110	17	-0.30890023129342126
1110	18	-0.07005346159174763
1110	19	-0.12701445327327576
1110	20	-0.3426760642029052
1110	21	-0.26829977247888337
1110	22	-0.23691817449449815
1110	23	-0.1669097225517291
1110	24	-0.09570310603832155
1110	25	-0.2974361808681252
1111	1	-0.2915309788902114
1111	2	-0.37292824786111467
1111	3	-0.43453011986529333
1111	4	-0.2624907669153984
1111	5	-0.3243026652613281
1111	6	-0.41284815705462563
1111	7	-0.36774308420687163
1111	8	-0.3935902775424367
1111	9	-0.5241131083101962
1111	10	-0.32271690026229294
1111	11	-0.32689654686740965
1111	12	-0.44433254170818515
1111	13	-0.5232466793046697
1111	14	-0.19993895471114342
1111	15	-0.2909195982739945
1111	16	-0.35217326259159165
1111	17	-0.3356358546896132
1111	18	-0.34013875574574115
1111	19	-0.4614372254240777
1111	20	-0.48116287342622144
1111	21	-0.44440537689754933
1111	22	-0.6307229525892382
1111	23	-0.47664482676511
1111	24	-0.6806854544607503
1111	25	-0.5571094490099497
1112	1	-0.5441607428825179
1112	2	-0.530667392437234
1112	3	-0.5620048067711174
1112	4	-0.4689806208903988
1112	5	-0.5714233141910725
1112	6	-0.6375858902629901
1112	7	-0.64202873615843
1112	8	-0.5605055109362738
1112	9	-0.7709520847677567
1112	10	-0.6520725537505001
1112	11	-0.670742943928083
1112	12	-0.565098737583142
1112	13	-0.7292881725928666
1112	14	-0.5949088219900389
1112	15	-0.6051916101762558
1112	16	-0.9168664057285696
1112	17	-0.8857947873106724
1112	18	-0.8299636785779967
1112	19	-0.8448749397151332
1112	20	-0.8064768803509921
1112	21	-0.7875522956672896
1112	22	-0.7136948915883679
1112	23	-0.7936419544405453
1112	24	-0.7907852186094502
1112	25	-0.7325743129431146
1113	1	-0.7691819861851066
1113	2	-0.8795993253394201
1113	3	-0.7917555696761873
1113	4	-0.6681762128767375
1113	5	-0.7774405978782539
1113	6	-0.8781236352419626
1113	7	-0.9982147934393026
1113	8	-1.0510532816947418
1113	9	-1.0110929602112506
1113	10	-0.9978688768242421
1113	11	-1.0102793972839947
1113	12	-0.8907417305469423
1113	13	-0.9591785397482582
1113	14	-0.9526513342460774
1113	15	-0.9559671515116008
1113	16	-1.0833673632850989
1113	17	-1.1215530729553436
1113	18	-1.1642398135151524
1113	19	-0.9882590980755381
1113	20	-1.239478568465259
1113	21	-1.1325514746883698
1113	22	-1.1244264859597024
1113	23	-1.4734590388228028
1113	24	-1.447095065892242
1113	25	-1.4268928361193076
1114	1	-2.3833683927354627
1114	2	-2.4912125513656527
1114	3	-2.120083975642558
1114	4	-5.277480876234158
1114	5	-2.85681226734318
1114	6	-2.2677316054101055
1114	7	-2.1916869446912166
1114	8	-2.360742857576195
1114	9	-2.9573595672628628
1114	10	-2.7270005652686535
1114	11	-2.3166376776937625
1114	12	-2.4565395685886457
1114	13	-2.4190197386192267
1114	14	-2.331758233776462
1114	15	-3.101285696660881
1114	16	-2.9799420828510037
1114	17	-2.7829258617233776
1114	18	-2.8105210201725725
1114	19	-2.85474874452175
1114	20	-3.1093603420740408
1114	21	-3.0162580104452346
1114	22	-3.115632504647202
1114	23	-3.273782055101357
1114	24	-3.2162152267312294
1114	25	-3.2951123837748533
1115	1	-2.6133965882241874
1115	2	-2.1643798445987343
1115	3	-1.6778847275222581
1115	4	-1.676447041647691
1115	5	-1.6260791846364135
1115	6	-1.6225248384928115
1115	7	-1.7607658920596378
1115	8	-1.8191545117115346
1115	9	-2.010461071022263
1115	10	-1.8231001893288052
1115	11	-1.8218068506260892
1115	12	-2.254706861821731
1115	13	-2.305062971701929
1115	14	-2.5758841736260862
1115	15	-2.5505338169616323
1115	16	-2.405928924956264
1115	17	-2.4081608241293964
1115	18	-2.2898443284432517
1115	19	-2.2725119024164897
1115	20	-2.236944928540204
1115	21	-2.2009384615730525
1115	22	-2.313705812917874
1115	23	-2.426507619011133
1115	24	-2.522606204174835
1115	25	-2.6853849401658323
1116	1	-1.3211751777110017
1116	2	-1.346550609940035
1116	3	-1.4672188707405667
1116	4	-1.2401957283207068
1116	5	-1.4581867461165317
1116	6	-1.5785889206414154
1116	7	-1.7423936787753362
1116	8	-1.6661303711198805
1116	9	-2.056130990909317
1116	10	-2.2239922256422986
1116	11	-2.0521038677321783
1116	12	-2.102250119529316
1116	13	-1.9459562803009405
1116	14	-2.7457630192116653
1116	15	-2.733713176386395
1116	16	-2.6872168883144383
1116	17	-2.344703531881308
1116	18	-2.315471647286209
1116	19	-2.261859589319336
1116	20	-2.2926012637012505
1116	21	-2.4835007625219347
1116	22	-2.398618558756276
1116	23	-2.652894410224974
1116	24	-2.7201213484766384
1116	25	-2.7846478353951696
1201	1	0.8898192142502985
1201	2	0.8982509759685335
1201	3	0.8181619453559001
1201	4	0.9152994806378487
1201	5	0.7948369385245115
1201	6	0.7779701399475272
1201	7	0.9153242793342642
1201	8	0.9304401612099653
1201	9	1.0539463903545254
1201	10	1.031485166351466
1201	11	0.9862093624570463
1201	12	1.0553411328046067
1201	13	0.9962021688150671
1201	14	1.4492918788971565
1201	15	1.287016696570035
1201	16	1.2031800325961157
1201	17	1.1590355800655274
1201	18	1.41969460354089
1201	19	1.3234180340962638
1201	20	1.247439483498539
1201	21	1.1826436926597879
1201	22	1.3848681616438867
1201	23	1.3760527263768338
1201	24	1.262961145395053
1201	25	1.3744414991312581
1202	1	0.9887942308952233
1202	2	0.9896131408486859
1202	3	0.8700147526359387
1202	4	1.0745635170380226
1202	5	1.0383215871139342
1202	6	0.9379790827553194
1202	7	0.9777933951182405
1202	8	1.0221804299691826
1202	9	1.1681068187576003
1202	10	1.2189221592750172
1202	11	1.1575576545199766
1202	12	1.1308929540008634
1202	13	1.2988294052335903
1202	14	1.5789423641591327
1202	15	1.4540604024957915
1202	16	1.3983589419830338
1202	17	1.2465357131430252
1202	18	1.4616633970725843
1202	19	1.3863545999434166
1202	20	1.3782132247287322
1202	21	1.379004575901277
1202	22	1.3812721210474734
1202	23	1.364059618562763
1202	24	1.3590594769717654
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2211	10	-0.52330370454915
2211	11	-0.49694165452581274
2211	12	-0.4852330082287679
2211	13	-0.41017000802644077
2211	14	-0.21334514880531685
2211	15	-0.4541881426323471
2211	16	-0.48612693747629976
2211	17	-0.546887542959908
2211	18	-0.3753087416401044
2211	19	-0.4807182360713469
2211	20	-0.48574922229570205
2211	21	-0.5656758920593958
2211	22	-0.6119952955169552
2211	23	-0.5963630956800401
2211	24	-0.5001453694047271
2211	25	-0.45757314722605
2212	1	-0.46647817464055663
2212	2	-0.4458803674289875
2212	3	-0.5381396695129972
2212	4	-0.40400424176719696
2212	5	-0.48078216725853196
2212	6	-0.39099973019225587
2212	7	-0.4738970228667867
2212	8	-0.41714939544404217
2212	9	-0.42708987398994225
2212	10	-0.584211888278162
2212	11	-0.6206401300166178
2212	12	-0.5152568294560425
2212	13	-0.5283643957919537
2212	14	-0.5377673584092335
2212	15	-0.5641663046377801
2212	16	-0.514008519638331
2212	17	-0.7226229936259827
2212	18	-0.6017047950070094
2212	19	-0.776927950815633
2212	20	-0.7186894388225369
2212	21	-0.7687570145780853
2212	22	-0.9594789792774847
2212	23	-0.9055415033909568
2212	24	-0.8072950579317464
2212	25	-0.7321906962779678
2213	1	-0.8586407477144569
2213	2	-0.7356602030770389
2213	3	-0.6916293441301669
2213	4	-0.5757254503704061
2213	5	-0.6007196642421349
2213	6	-0.6693940095861635
2213	7	-0.8506682116251483
2213	8	-0.7743645909802837
2213	9	-0.9375621373673653
2213	10	-1.0323671876246863
2213	11	-1.0130626915736727
2213	12	-0.9737977844462478
2213	13	-1.0975292658439955
2213	14	-0.8609732064807574
2213	15	-0.9971028841964582
2213	16	-1.1050442665947742
2213	17	-1.23427972244086
2213	18	-1.0411154679446994
2213	19	-1.0801819554316268
2213	20	-1.0885955873325415
2213	21	-1.1364708635073129
2213	22	-1.1410645443025942
2213	23	-1.0332832276694006
2213	24	-0.9868953665461788
2213	25	-1.0916351043603854
2214	1	-1.2059440494199052
2214	2	-0.9911537419973477
2214	3	-1.0513973841345212
2214	4	-0.8954098336370322
2214	5	-0.9845494060809159
2214	6	-1.1336156031884386
2214	7	-1.1084865971823135
2214	8	-1.1987842778559994
2214	9	-1.3093572923470518
2214	10	-1.2466401436104348
2214	11	-1.2929410231271135
2214	12	-1.3003880426478247
2214	13	-1.4783932697603532
2214	14	-1.2681222612356464
2214	15	-1.430483541278278
2214	16	-1.4175822020849296
2214	17	-1.4094386213208026
2214	18	-1.3799688326621222
2214	19	-1.517418868086338
2214	20	-1.501267127739915
2214	21	-1.5511989172075786
2214	22	-1.6670110424515556
2214	23	-1.5343835852672072
2214	24	-1.6575698534936407
2214	25	-1.6240123856233097
2215	1	-1.4544531970342653
2215	2	-1.689088002430978
2215	3	-1.5791391399722876
2215	4	-1.782699652961064
2215	5	-1.6061126808680868
2215	6	-1.481084333138952
2215	7	-1.614875273504225
2215	8	-1.734329958491628
2215	9	-2.1188065272477203
2215	10	-1.7701870014374421
2215	11	-1.908332105374221
2215	12	-2.0565798207567028
2215	13	-2.013711485351287
2215	14	-2.8274523007832073
2215	15	-2.475778220269831
2215	16	-2.3388870252057004
2215	17	-2.210732086201702
2215	18	-2.18374951187797
2215	19	-2.367502890037521
2215	20	-2.647118203689608
2215	21	-2.401392696185951
2215	22	-2.754954944711976
2215	23	-2.296796207080689
2215	24	-2.491191044183907
2215	25	-2.4007415161224657
2216	1	-1.6649117860859057
2216	2	-1.5454355522841752
2216	3	-1.4974150741313892
2216	4	-1.45224869266384
2216	5	-1.9586304229200664
2216	6	-1.6321351401752793
2216	7	-1.7016677039466792
2216	8	-2.1613298381493316
2216	9	-2.0722943120149537
2216	10	-2.1202426522647357
2216	11	-1.9937971238394923
2216	12	-2.231911577139705
2216	13	-2.1333374956809124
2216	14	-2.0796159991789622
2216	15	-2.1196435146243076
2216	16	-2.2615101410609313
2216	17	-2.345512027171381
2216	18	-2.543074186408127
2216	19	-2.380384904024332
2216	20	-2.5342396350520744
2216	21	-2.556289939051993
2216	22	-2.764548963367922
2216	23	-2.430174712771205
2216	24	-2.5958430113830957
2216	25	-2.6399563618440283
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105022.0
3	7.0
4	727.0
5	4446.0
6	5758.0
7	5352.0
8	4749.0
9	4550.0
10	4123.0
11	4092.0
12	3826.0
13	3758.0
14	3752.0
15	3693.0
16	3894.0
17	4025.0
18	4587.0
19	5459.0
20	7092.0
21	8989.0
22	11458.0
23	13754.0
24	16068.0
25	18193.0
26	20475.0
27	22986.0
28	26060.0
29	31006.0
30	39053.0
31	48820.0
32	62449.0
33	81898.0
34	109605.0
35	151940.0
36	220690.0
37	382190.0
38	1076984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.4602922083859	17.651878158786644	12.239933025971517	25.647896606855937
2	16.051147194900768	20.473170239004425	38.79571183732033	24.67997072877447
3	18.657732320933835	24.90762253786518	28.92923582999992	27.505409311201067
4	12.44813774996886	16.732702202887996	38.077711130062056	32.74144891708109
5	14.860145933636254	36.018846067686276	34.15009210198983	14.970915896687643
6	31.906830479861686	36.999906945500015	17.323433080036054	13.76982949460224
7	28.219713760623755	31.032388227429237	22.28878085092278	18.459117161024224
8	26.017835735843903	33.96281266647126	20.447203202956967	19.57214839472787
9	26.702303225937836	15.398164079642	19.233040656743427	38.66649203767673
10	15.266527840724184	27.393859048410057	32.795093308203576	24.544519802662183
11	35.01968288551341	22.587079497453484	23.43277669648583	18.96046092054727
12	24.00834439476782	24.334676678662532	29.80813003455001	21.848848892019635
13	28.928522940542265	21.032304436369444	25.259673361817676	24.779499261270615
14	22.69387243363763	20.390909210694964	26.85311736739405	30.06210098827336
15	24.193932888736082	27.609233598837836	23.304977979777895	24.891855532648187
16	24.349105384306966	27.037305338621742	24.472423537154388	24.141165739916904
17	23.8794734902139	26.277048998986867	25.662619857809933	24.180857652989303
18	24.375173953791485	25.722200237435693	26.514251532490473	23.38837427628235
19	25.513713946185618	25.682702474771514	25.74794993595047	23.055633643092392
20	24.677635125691353	25.762274624123954	25.866381806201705	23.693708443982988
21	24.847127376349178	25.592911773737736	25.709036266541652	23.850924583371437
22	24.211431325670706	25.505383179943685	26.075627678262304	24.207557816123305
23	23.917626391566163	25.219849183520004	26.03606960256826	24.826454822345568
24	23.778065583839624	25.949150978773574	26.121195638500144	24.151587798886663
25	24.21570467386747	25.52490666240741	25.91790284904267	24.341485814682454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9470.0
1	9470.0
2	8872.5
3	8275.0
4	8275.0
5	8275.0
6	6322.5
7	4370.0
8	4370.0
9	4370.0
10	5655.5
11	6941.0
12	6941.0
13	6941.0
14	9811.5
15	12682.0
16	12682.0
17	12682.0
18	21073.0
19	29464.0
20	29464.0
21	29464.0
22	42474.5
23	55485.0
24	55485.0
25	55485.0
26	74852.5
27	94220.0
28	94220.0
29	94220.0
30	115413.0
31	136606.0
32	136606.0
33	136606.0
34	160186.5
35	183767.0
36	183767.0
37	183767.0
38	206884.5
39	230002.0
40	230002.0
41	230002.0
42	252153.5
43	274305.0
44	274305.0
45	274305.0
46	290772.0
47	307239.0
48	307239.0
49	307239.0
50	310243.5
51	313248.0
52	313248.0
53	313248.0
54	293229.5
55	273211.0
56	273211.0
57	273211.0
58	245059.0
59	216907.0
60	216907.0
61	216907.0
62	185716.0
63	154525.0
64	154525.0
65	154525.0
66	126074.0
67	97623.0
68	97623.0
69	97623.0
70	75196.5
71	52770.0
72	52770.0
73	52770.0
74	40882.5
75	28995.0
76	28995.0
77	28995.0
78	23059.5
79	17124.0
80	17124.0
81	17124.0
82	12858.5
83	8593.0
84	8593.0
85	8593.0
86	6257.5
87	3922.0
88	3922.0
89	3922.0
90	2753.5
91	1585.0
92	1585.0
93	1585.0
94	878.5
95	172.0
96	172.0
97	172.0
98	100.5
99	29.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23509535877027044
2	0.6615427934626992
3	0.6579338734815767
4	0.6669760026650486
5	0.6837515318080689
6	0.6988217471138555
7	0.6929126363755339
8	0.6878363533251637
9	0.6810547564375597
10	0.6819272425868421
11	0.6870431840985434
12	0.6874397687118535
13	0.6833152887334277
14	0.6766130087684858
15	0.6837515318080689
16	0.6915642486902793
17	0.690731421002328
18	0.6850206025706616
19	0.6828393871974555
20	0.6892640579330803
21	0.6869242087145503
22	0.6877173779411706
23	0.6911280056156381
24	0.6951731686714019
25	0.6813323656668768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2521530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.21600918771833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.61167082400743	58.0129520023383
2	7.402536247139628	9.80332816306031
3	1.8509190945173608	3.6768142730485454
4	0.8588632537476091	2.274819884045814
5	0.5034611548029617	1.6668594226046096
6	0.31934651258036406	1.2687510966652316
7	0.2375670692892224	1.1011520269928126
8	0.1734885616622238	0.9190176154391877
9	0.1342788082801814	0.8002266122516724
>10	0.8047008265985555	9.84297553306308
>50	0.0596814766923398	2.7274220452055578
>100	0.03950587672707914	5.075375185956292
>500	0.0030153742083971857	1.3267409443708431
>1k	8.443047783475903E-4	1.0412584313855602
>5k	1.2061496833537005E-4	0.46230676357241146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNN	5811	0.2304553187945414	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5766	0.22867068803464563	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	4508	0.1787803436802259	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3285	0.13027804547239177	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.965846133101728E-5
2	0.0	0.0	0.0	0.0	3.965846133101728E-5
3	0.0	0.0	0.0	0.0	3.965846133101728E-5
4	0.0	0.0	0.0	0.0	3.965846133101728E-5
5	0.0	0.0	0.0	7.931692266203455E-5	7.931692266203455E-5
6	0.0	0.0	0.0	7.931692266203455E-5	1.1897538399305184E-4
7	0.0	0.0	0.0	7.931692266203455E-5	1.1897538399305184E-4
8	0.0	0.0	0.0	7.931692266203455E-5	1.1897538399305184E-4
9	0.0	0.0	0.0	7.931692266203455E-5	1.1897538399305184E-4
10	0.0	0.0	0.0	1.1897538399305184E-4	1.1897538399305184E-4
11	0.0	0.0	0.0	1.1897538399305184E-4	1.1897538399305184E-4
12	0.0	0.0	0.0	1.1897538399305184E-4	2.3795076798610368E-4
13	0.0	0.0	0.0	1.1897538399305184E-4	2.3795076798610368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	80	4.3291948E-10	16.624353	16
GGTATCA	950	0.0	14.001693	1
GTATCAA	3195	0.0	13.530587	1
CCGGTAG	105	1.9967047E-8	12.666174	9
GCGTTAT	70	1.090183E-4	12.215762	1
ACGAACG	105	2.7243186E-7	11.761211	15
TAAACGC	185	0.0	11.297084	18
CTTATAC	230	0.0	11.153076	3
AACCGCG	140	8.358256E-9	10.857154	7
ACGCTTA	70	0.0014924381	10.856721	17
AAATTCG	70	0.0014926869	10.8565035	14
AACGAAC	125	2.2435597E-7	10.639374	14
CGGTAGC	135	5.695074E-8	10.555145	10
ATAGCAC	155	3.6616257E-9	10.420198	3
GTATTAG	350	0.0	10.315534	1
AGGCCCG	215	1.8189894E-12	10.162395	10
CGTTATT	85	6.588612E-4	10.06004	2
CCCCTAT	180	3.929017E-10	10.028991	1
CGCTTAC	95	1.645047E-4	9.999812	18
GTCTAGC	115	1.0254671E-5	9.914242	1
>>END_MODULE