FastQCFastQC Report
Thu 2 Feb 2017
SRR4050243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521530
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90960.36073336426693314No Hit
TATCAACGCAGAGTACTTTTTTTTT62380.2473894817828858No Hit
GGTATCAACGCAGAGTACTTTTTTT33820.13412491622150044No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32160.12754161164055158No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28790.11417671017199874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.014.9464251
GTATCAA37000.012.932821
TATAACG751.4770187E-512.6686912
CGTCGTA2050.012.04640310
AACCGCG951.0438471E-611.9969187
ATACCGT2150.011.9269326
TATACTG3450.011.5672955
GCTCGTA1451.2223609E-911.1357339
ACGAACG700.001494912310.8545715
CGAACGT1502.1282176E-910.76864
ACCGTCC1502.1445885E-910.7639028
TACCGTC2300.010.7363737
TAGATCG1002.3952447E-510.4518775
ACTGCGT1106.0674447E-610.3609758
ATTACCG2600.010.22834515
ACCGTCG2053.6379788E-1210.1925048
TACTCCG750.002644604810.1351545
ACTATAC2350.010.1071963
ACGGAAT1803.9835868E-1010.02321418
ATAAGAC2002.5465852E-119.9758013
Produced by FastQC (version 0.11.5)