##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2521530
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.705412190217842	31.0	31.0	34.0	27.0	34.0
2	30.75957176793455	31.0	31.0	34.0	27.0	34.0
3	30.993233473327702	31.0	31.0	34.0	27.0	34.0
4	34.626117079709545	37.0	35.0	37.0	31.0	37.0
5	34.52229400403723	37.0	35.0	37.0	32.0	37.0
6	34.471627940179175	37.0	35.0	37.0	31.0	37.0
7	34.4033638306901	37.0	35.0	37.0	30.0	37.0
8	34.382727550336504	37.0	35.0	37.0	30.0	37.0
9	35.97989831570515	39.0	37.0	39.0	31.0	39.0
10	35.86206271589075	39.0	37.0	39.0	30.0	39.0
11	35.85704750687083	39.0	37.0	39.0	30.0	39.0
12	35.78103849646841	39.0	37.0	39.0	30.0	39.0
13	35.7855968400138	39.0	37.0	39.0	30.0	39.0
14	36.944924708411165	40.0	37.0	41.0	31.0	41.0
15	36.92029045857079	40.0	37.0	41.0	31.0	41.0
16	36.89908864855861	40.0	37.0	41.0	31.0	41.0
17	36.87968376342935	40.0	37.0	41.0	31.0	41.0
18	36.84420213124571	40.0	37.0	41.0	31.0	41.0
19	36.90199997620493	40.0	37.0	41.0	31.0	41.0
20	36.85606358044521	40.0	37.0	41.0	31.0	41.0
21	36.80852775894001	40.0	37.0	41.0	31.0	41.0
22	36.7534294654436	40.0	37.0	41.0	30.0	41.0
23	36.66285310902508	40.0	37.0	41.0	30.0	41.0
24	36.612192200767	40.0	37.0	41.0	30.0	41.0
25	36.39107169060055	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8598934106500096
1101	2	1.0328326992950139
1101	3	0.9405286893303817
1101	4	0.9026628132817862
1101	5	0.934163893118793
1101	6	1.037064660862562
1101	7	0.9125474383646335
1101	8	1.010916316472894
1101	9	1.0915459485978616
1101	10	1.0806030282308754
1101	11	1.0358910720282992
1101	12	1.0175439777330055
1101	13	1.158123672952847
1101	14	1.2803665939381048
1101	15	1.4048626122810148
1101	16	1.3649501216526119
1101	17	1.3274983331677106
1101	18	1.413516630962846
1101	19	1.2776755829508772
1101	20	1.3578960154333117
1101	21	1.359979731523886
1101	22	1.4104248877211276
1101	23	1.4267398817494836
1101	24	1.4490016523438811
1101	25	1.5241144280443066
1102	1	0.5899814567900066
1102	2	0.7146366646397304
1102	3	0.6946815909309016
1102	4	0.5457694692883805
1102	5	0.7225391376215669
1102	6	0.6644736102926032
1102	7	0.7291839350233005
1102	8	0.7049499459751374
1102	9	0.7821467340237405
1102	10	0.846131279929196
1102	11	0.8867803330139523
1102	12	0.8846722455219975
1102	13	0.9001411916708193
1102	14	1.0970966176215597
1102	15	1.0467192242896814
1102	16	1.0779956331096727
1102	17	1.091059248928019
1102	18	1.1058217704237023
1102	19	1.1672365087678571
1102	20	1.058436309993155
1102	21	1.0701510468859468
1102	22	1.1126081906330327
1102	23	1.1960036832710585
1102	24	1.2277089177846392
1102	25	1.1740583621127811
1103	1	0.5355180783799405
1103	2	0.6536431429124754
1103	3	0.5986862887160527
1103	4	0.5073736644838647
1103	5	0.5619121357793162
1103	6	0.6744827009121792
1103	7	0.5910931291910231
1103	8	0.6358882581114997
1103	9	0.7153601136400951
1103	10	0.6625157224242741
1103	11	0.672768619432162
1103	12	0.7323942346885275
1103	13	0.7759097205634546
1103	14	0.8862736173889871
1103	15	0.9126214875837491
1103	16	0.9311087843025021
1103	17	0.9073435691532978
1103	18	0.8633010246068835
1103	19	0.8281629123855794
1103	20	0.8494282973164715
1103	21	0.859489448577456
1103	22	0.8606523081239885
1103	23	0.9969241808811518
1103	24	0.9439836359223577
1103	25	1.0020753235490645
1104	1	0.5188788766954211
1104	2	0.6740401305803587
1104	3	0.5980878233643132
1104	4	0.5424440731695768
1104	5	0.5240986196173623
1104	6	0.692759629309954
1104	7	0.5801418914019223
1104	8	0.6273571599530143
1104	9	0.6534231881583565
1104	10	0.7933117661105484
1104	11	0.7409224640210468
1104	12	0.7306293552801435
1104	13	0.750454598683362
1104	14	0.8328991543972037
1104	15	0.8484568468833587
1104	16	0.909896324016529
1104	17	0.8030690346169322
1104	18	0.8156001148717351
1104	19	0.8117932744147325
1104	20	0.861862135530771
1104	21	0.8731015684332846
1104	22	0.8267652671876036
1104	23	0.8961302394527735
1104	24	0.890113277419573
1104	25	1.0702003468603891
1105	1	0.37727188163577097
1105	2	0.5928054774288931
1105	3	0.46581297582469716
1105	4	0.6224908565518916
1105	5	0.6064407173399218
1105	6	0.6848778112396303
1105	7	0.5987010836415578
1105	8	0.5333415156910561
1105	9	0.6331285360995409
1105	10	0.671861169072379
1105	11	0.7387208799717015
1105	12	0.7578663669265566
1105	13	0.8126833355283196
1105	14	0.7903979307374769
1105	15	0.841972583872149
1105	16	0.8722762855060893
1105	17	0.8840656083697027
1105	18	0.8029950631732774
1105	19	0.8359837914513832
1105	20	0.8791405453417553
1105	21	0.8247622335615645
1105	22	0.7860336208756209
1105	23	0.8048120355213726
1105	24	0.8946730590971228
1105	25	1.0016769631597242
1106	1	0.2800171588948501
1106	2	0.5014311964173288
1106	3	0.4099241436654282
1106	4	0.3353257150392537
1106	5	0.3702476160585064
1106	6	0.5007564320671776
1106	7	0.47066205207568856
1106	8	0.4298403856608175
1106	9	0.4531431033140265
1106	10	0.5032881491245789
1106	11	0.5037335013205251
1106	12	0.44335520765477554
1106	13	0.4854215791078573
1106	14	0.5288668022425043
1106	15	0.504051768108134
1106	16	0.5502808627533824
1106	17	0.4047599789267906
1106	18	0.45770030915568327
1106	19	0.4672472473802003
1106	20	0.5701574297430483
1106	21	0.5936016050291073
1106	22	0.49366641281795154
1106	23	0.5593341202040207
1106	24	0.5591908236009573
1106	25	0.5087770520625767
1107	1	0.24016574864774398
1107	2	0.41166474741392634
1107	3	0.4210906937186216
1107	4	0.3511402818190632
1107	5	0.3446175267704703
1107	6	0.48002602552659823
1107	7	0.35035049556653775
1107	8	0.3672589330905467
1107	9	0.39086480012904445
1107	10	0.37246536006808384
1107	11	0.3917819668834923
1107	12	0.3759977145660045
1107	13	0.35956775626266335
1107	14	0.3869557835702011
1107	15	0.40232419999879454
1107	16	0.4585553074245112
1107	17	0.39552775198373524
1107	18	0.4299852805317599
1107	19	0.39478441193390523
1107	20	0.4881364886107207
1107	21	0.5082197312811019
1107	22	0.36875996603271943
1107	23	0.49190556364146687
1107	24	0.39142844671260946
1107	25	0.47258506840580594
1108	1	0.2822967995322365
1108	2	0.3019344590094093
1108	3	0.31213947407508513
1108	4	0.33908996696705884
1108	5	0.2797945795539363
1108	6	0.31238603186790215
1108	7	0.43254037819332325
1108	8	0.38723876648555233
1108	9	0.3688514941626124
1108	10	0.32842436593352886
1108	11	0.3651441591881124
1108	12	0.40987902948539556
1108	13	0.31358398280112
1108	14	0.409822827536523
1108	15	0.4236633156339309
1108	16	0.42492827508478825
1108	17	0.4818822999183041
1108	18	0.4469711861054435
1108	19	0.42153589812758696
1108	20	0.4224119940663016
1108	21	0.4295650463675571
1108	22	0.486007351854127
1108	23	0.572637655797358
1108	24	0.5506821063262208
1108	25	0.491902729473658
1109	1	0.06461848589288621
1109	2	0.14329899817331793
1109	3	0.1324893797318154
1109	4	0.14469593788635393
1109	5	0.24283352810953573
1109	6	0.15765193964259083
1109	7	0.19356317284745472
1109	8	0.11372498965568667
1109	9	0.2288105374376741
1109	10	0.25507455419780456
1109	11	0.24194538881115335
1109	12	0.19875484108957409
1109	13	0.27622690204005806
1109	14	0.2176956344362111
1109	15	0.41786292566980876
1109	16	0.23108727951186836
1109	17	0.2447560038020029
1109	18	0.2640266268828739
1109	19	0.2289418510816077
1109	20	0.2824692449344326
1109	21	0.26084657676303635
1109	22	0.3397994701157714
1109	23	0.2689509875389007
1109	24	0.26653279905122673
1109	25	0.16184230013437428
1110	1	-0.11158972891553631
1110	2	0.08451275653454715
1110	3	0.046345785079747515
1110	4	0.05108302499393602
1110	5	0.028947698435970892
1110	6	-0.1666520604638677
1110	7	-0.01507655092861171
1110	8	0.08447902126884088
1110	9	0.014022940899884873
1110	10	0.016305331352398866
1110	11	-0.02207977514813564
1110	12	0.029985879465158405
1110	13	-0.02369415254354834
1110	14	0.0068305420120395866
1110	15	0.05340525616018965
1110	16	0.006019343574095615
1110	17	-0.08306468376612486
1110	18	-0.1527245874866452
1110	19	-0.08341714195587002
1110	20	-0.10745660773644516
1110	21	-0.1417457211116897
1110	22	-0.1400006317604081
1110	23	-0.14368883037528946
1110	24	-0.11402119643457809
1110	25	-0.07657384312512505
1111	1	-0.3453562444724838
1111	2	-0.2029677436515982
1111	3	-0.27862193061390883
1111	4	-0.2300933113390613
1111	5	-0.2274408348003476
1111	6	-0.563453873432394
1111	7	-0.3402973312599755
1111	8	-0.2864163291934787
1111	9	-0.36504549231498373
1111	10	-0.3282270887977887
1111	11	-0.4226960647018885
1111	12	-0.3814479080753017
1111	13	-0.31513074572496436
1111	14	-0.27281435295424217
1111	15	-0.21721198427146504
1111	16	-0.3479231708383139
1111	17	-0.41423323728822226
1111	18	-0.3791258894061613
1111	19	-0.40019761905755047
1111	20	-0.3892223259251253
1111	21	-0.46629624565225924
1111	22	-0.4896934355408078
1111	23	-0.47621332766458835
1111	24	-0.502813008882697
1111	25	-0.4395309585317122
1112	1	-0.4319488518529475
1112	2	-0.35404748534552866
1112	3	-0.4282309757898659
1112	4	-0.3350940857853075
1112	5	-0.41368410932047794
1112	6	-1.1753741010722791
1112	7	-0.6292967383619654
1112	8	-0.5040721229836294
1112	9	-0.487246709534098
1112	10	-0.5463488304119082
1112	11	-0.5987573329448566
1112	12	-0.5512421446925373
1112	13	-0.5043156359030334
1112	14	-0.5696014111810754
1112	15	-0.5999283476611197
1112	16	-0.5745200759362916
1112	17	-0.6897497105640937
1112	18	-0.7184975288228728
1112	19	-0.6441675546257386
1112	20	-0.6489467187890412
1112	21	-0.6874696338426176
1112	22	-0.7239936736851149
1112	23	-0.6691829586302447
1112	24	-0.789128930523546
1112	25	-0.7544574790830296
1113	1	-0.6047017341699714
1113	2	-0.7452549145616345
1113	3	-0.6118510464167528
1113	4	-0.503191223103677
1113	5	-0.5906039325943553
1113	6	-1.1077723507603494
1113	7	-0.6639776121256702
1113	8	-0.6369261862239739
1113	9	-0.7530051540779183
1113	10	-0.7510691943968908
1113	11	-0.815640669948003
1113	12	-0.78211049826281
1113	13	-0.9094501969971063
1113	14	-0.998207267900959
1113	15	-0.9152122512532301
1113	16	-0.9321275239176288
1113	17	-0.8848241396810721
1113	18	-0.955629535423995
1113	19	-0.7730211426819693
1113	20	-0.7743086872619429
1113	21	-0.8355227787972268
1113	22	-0.8902937373204409
1113	23	-0.8270004561502802
1113	24	-0.8797615988261143
1113	25	-0.8199163609021696
1114	1	-0.9531966060584303
1114	2	-1.0414101996189373
1114	3	-1.046047125205959
1114	4	-0.9798455409370987
1114	5	-1.0579152631051016
1114	6	-1.5494119473245718
1114	7	-1.236403062888499
1114	8	-1.336739280853756
1114	9	-1.466938804952683
1114	10	-1.6312756267398782
1114	11	-1.457890256292508
1114	12	-1.5367010097356655
1114	13	-1.5428213980580168
1114	14	-1.801964233129567
1114	15	-1.7783401863823087
1114	16	-1.745873813184481
1114	17	-1.7348398038414743
1114	18	-1.916621213648689
1114	19	-1.7236767562304038
1114	20	-1.7478005175658566
1114	21	-1.8257136686432034
1114	22	-1.814580333665674
1114	23	-1.819385849244668
1114	24	-1.884885147990289
1114	25	-1.8118713697835958
1115	1	-1.2856684105697056
1115	2	-1.9279233575136736
1115	3	-1.5686035161834013
1115	4	-1.2653248642453718
1115	5	-1.2740806766389383
1115	6	-1.7669871352944995
1115	7	-1.4060459200313602
1115	8	-1.3914855214552588
1115	9	-1.5327282786368883
1115	10	-1.5133244989203334
1115	11	-1.5732567976458895
1115	12	-1.4871239420664963
1115	13	-1.5975676386595197
1115	14	-1.731005586513028
1115	15	-1.7522593593146425
1115	16	-1.933373813184481
1115	17	-1.7858266459467416
1115	18	-1.90604790537801
1115	19	-1.7941654780349197
1115	20	-1.8993512694455532
1115	21	-1.982903518267264
1115	22	-2.138358529154395
1115	23	-1.980570059770983
1115	24	-2.057582516411337
1115	25	-2.133534903618184
1116	1	-0.8668258620586933
1116	2	-1.019589476960764
1116	3	-1.0371277881077532
1116	4	-0.9054106819555798
1116	5	-0.8195925868730356
1116	6	-1.1588264497378091
1116	7	-1.2568427401383957
1116	8	-1.2768936378160305
1116	9	-1.4367476748348125
1116	10	-1.4844343833475548
1116	11	-1.381798348028397
1116	12	-1.3670879076573286
1116	13	-1.4585991749237621
1116	14	-1.6261261663468929
1116	15	-1.594656016897467
1116	16	-1.5520421406625644
1116	17	-1.5564376713267833
1116	18	-1.6280217127129433
1116	19	-1.6334316976231875
1116	20	-1.5564621142431392
1116	21	-1.6405386678223692
1116	22	-1.641289916027688
1116	23	-1.559459970136416
1116	24	-1.607344270687861
1116	25	-1.8345796141937498
1201	1	0.561266921275756
1201	2	0.7440247042834685
1201	3	0.7199588930818344
1201	4	0.6097502966395538
1201	5	0.6716573626234847
1201	6	0.7369163554207603
1201	7	0.6967149898080791
1201	8	0.7474036683781122
1201	9	0.8652765045747373
1201	10	0.8790677298504406
1201	11	0.8577121954420193
1201	12	0.9286517182658969
1201	13	0.8719354598648934
1201	14	1.0529663209212643
1201	15	1.0960662457643338
1201	16	1.0466933847093856
1201	17	1.0338392334491573
1201	18	1.0147805977963529
1201	19	1.0425667975387398
1201	20	1.069449514936153
1201	21	1.1614139125576415
1201	22	1.0446598507290332
1201	23	0.9761491879159578
1201	24	1.1003380866091774
1201	25	1.1325290765772635
1202	1	0.5504693816944126
1202	2	0.725221386272473
1202	3	0.6262954292834202
1202	4	0.6488279917864404
1202	5	0.6499077497766308
1202	6	0.7428109105447405
1202	7	0.7390554176051438
1202	8	0.8553749694993229
1202	9	0.9002453398722992
1202	10	0.8229535763830143
1202	11	0.9584213961335308
1202	12	0.9081199487774114
1202	13	0.9637091413125773
1202	14	1.0598845490988111
1202	15	1.1116894620140414
1202	16	1.059069264351642
1202	17	1.174405349215931
1202	18	1.1327789812118638
1202	19	1.1846325533820732
1202	20	1.119885067346985
1202	21	1.1790117531807525
1202	22	1.1634214028794432
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2211	5	-0.5056911491980927
2211	6	-0.469120551595509
2211	7	-0.5157051649244764
2211	8	-0.4901363012384863
2211	9	-0.5314348989046564
2211	10	-0.5903194562270428
2211	11	-0.5416888155612014
2211	12	-0.5773696758579305
2211	13	-0.5843864538383201
2211	14	-0.783996323076984
2211	15	-0.7597716930301246
2211	16	-0.7480906614766596
2211	17	-0.780913663942286
2211	18	-0.7249438194280131
2211	19	-0.7418952297024433
2211	20	-0.7300278987708211
2211	21	-0.7618397765380251
2211	22	-0.6769980606069268
2211	23	-0.7398245809446777
2211	24	-0.6727553823264216
2211	25	-0.6307090586227204
2212	1	-0.6054755857925329
2212	2	-0.5533466010529864
2212	3	-0.5956055203660462
2212	4	-0.5446049145366629
2212	5	-0.6136947158761004
2212	6	-0.610200300658235
2212	7	-0.6713900543738376
2212	8	-0.5824243752121703
2212	9	-0.6597464656607457
2212	10	-0.8629003217555677
2212	11	-0.7337532374208209
2212	12	-0.8952022919146216
2212	13	-0.84026357552154
2212	14	-0.8867374243858563
2212	15	-0.808083937315935
2212	16	-0.8230986426422362
2212	17	-0.7964450794477074
2212	18	-0.8185843167707318
2212	19	-0.9248154680762468
2212	20	-0.7924387065814429
2212	21	-0.8711314349131811
2212	22	-0.8525617731916384
2212	23	-0.929892870108084
2212	24	-0.9579849089821693
2212	25	-0.9095721614979837
2213	1	-0.5015983691138679
2213	2	-0.8866126646389283
2213	3	-0.576980229717762
2213	4	-0.6118902535221977
2213	5	-0.6879449253985044
2213	6	-0.6308610934253238
2213	7	-0.7297092340641669
2213	8	-0.5899339048175776
2213	9	-0.7478357866627192
2213	10	-0.7386976693551546
2213	11	-0.6749221244850006
2213	12	-0.7453920135379732
2213	13	-0.8254021401823692
2213	14	-0.9460062038845436
2213	15	-0.9511631089052486
2213	16	-0.9560436625983755
2213	17	-0.9701790363567255
2213	18	-1.0532015271157888
2213	19	-0.9516530671159344
2213	20	-0.9923013683125674
2213	21	-0.9149954422596309
2213	22	-0.9133984722161017
2213	23	-0.9798191433464467
2213	24	-0.9901692133248332
2213	25	-0.9100967112644867
2214	1	-0.8871403721993723
2214	2	-0.8994142921788324
2214	3	-0.912522077366269
2214	4	-0.9038175157699868
2214	5	-1.0465802387319485
2214	6	-1.0482312488563252
2214	7	-0.9702235549111151
2214	8	-1.044115031897249
2214	9	-1.1408955410624486
2214	10	-1.239358058439592
2214	11	-1.2553094767564517
2214	12	-1.2319534488115806
2214	13	-1.1924368871142974
2214	14	-1.4125914215642439
2214	15	-1.4708733110753371
2214	16	-1.5707258160258135
2214	17	-1.4334968047199297
2214	18	-1.4386844641420495
2214	19	-1.3484784327760195
2214	20	-1.407666441008267
2214	21	-1.52567190243888
2214	22	-1.4288829150723643
2214	23	-1.5494895781877247
2214	24	-1.590750394987431
2214	25	-1.613697772010191
2215	1	-1.458629644395188
2215	2	-3.133405821093664
2215	3	-1.729223671862318
2215	4	-1.5080210542275694
2215	5	-1.4146561007430094
2215	6	-1.2980769412627566
2215	7	-1.377594712310085
2215	8	-1.48477882060196
2215	9	-1.6463717995443616
2215	10	-1.6993449220691588
2215	11	-1.8073719775452943
2215	12	-1.5862689266485859
2215	13	-1.688505734016644
2215	14	-1.8240572833757014
2215	15	-1.9580060788801177
2215	16	-2.1486691795093407
2215	17	-2.131920746074762
2215	18	-1.8883152462843
2215	19	-1.824464245762833
2215	20	-1.9214262413683585
2215	21	-1.9298303037360398
2215	22	-1.9796102611024082
2215	23	-2.134318388084047
2215	24	-2.307052944291776
2215	25	-2.346470825559109
2216	1	-1.2146451398671658
2216	2	-1.8927249874192889
2216	3	-1.4051953215908028
2216	4	-1.2313608680469912
2216	5	-1.2383251930698265
2216	6	-1.209996015505567
2216	7	-1.3109964373687077
2216	8	-1.322201823441027
2216	9	-1.399648813844955
2216	10	-1.4732169062795535
2216	11	-1.3823335521036597
2216	12	-1.3392570323558175
2216	13	-1.5682982053352035
2216	14	-1.5242469626441633
2216	15	-1.6600815017668111
2216	16	-1.6450241033897086
2216	17	-1.596206652805506
2216	18	-1.8634672566728199
2216	19	-1.7127132261870983
2216	20	-1.722431847925904
2216	21	-1.8497157004244684
2216	22	-1.7645602678869707
2216	23	-1.7906627048606722
2216	24	-1.8314402749206096
2216	25	-1.9213107515292762
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35853.0
3	2.0
4	650.0
5	5283.0
6	6524.0
7	5284.0
8	4364.0
9	3680.0
10	3289.0
11	3054.0
12	2771.0
13	2665.0
14	2665.0
15	2625.0
16	2847.0
17	3063.0
18	3158.0
19	4060.0
20	5314.0
21	7180.0
22	9147.0
23	11350.0
24	13475.0
25	15469.0
26	17611.0
27	20401.0
28	23421.0
29	28642.0
30	37034.0
31	48222.0
32	62694.0
33	83242.0
34	112334.0
35	154936.0
36	225923.0
37	392720.0
38	1160578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55258697976584	18.326265495754722	12.999621571913075	26.121525952566365
2	15.810555415634612	21.074976252000486	37.85873361189721	25.255734720467704
3	18.311422033447947	25.13275669930558	29.258109163880658	27.29771210336581
4	12.906806581718241	17.34661098618696	37.25813295895746	32.488449473137344
5	14.616688813826176	36.41056508784996	34.12326411965736	14.849481978666512
6	31.70142905810979	37.55516169062972	17.05851115092064	13.684898100339844
7	28.03668227153098	31.233987299300875	22.22424047249266	18.505089956675484
8	25.921309368952063	34.340397855261905	20.285700688473618	19.452592087312414
9	26.548713632646198	15.369815245077314	19.28531709611682	38.79615402615967
10	15.103214088664597	27.798013501147707	32.677037458366705	24.421734951820998
11	35.14286258206835	22.620920180822043	23.331643778352596	18.90457345875701
12	23.928898076454754	24.482663978087338	29.88666083365526	21.70177711180265
13	29.012143924705764	21.100862482031623	25.30994456379289	24.577049029469723
14	22.832715762705345	20.410646460215773	26.699832912872374	30.05680486420651
15	24.11796486139561	27.69315046323108	23.484126064860288	24.70475861051302
16	24.258297732761502	27.070858590205454	24.583769788213118	24.087073888819923
17	23.809049766218273	26.200607685745325	25.864123815276542	24.126218732759863
18	24.37125325795035	25.67364022710075	26.64832855051152	23.30677796443738
19	25.312959563926878	25.539895899599113	26.181361871840064	22.965782664633945
20	24.57728017260184	25.606823166402926	26.282913131938084	23.532983529057155
21	24.75067014772003	25.360674117643793	26.105322355033334	23.78333337960284
22	24.058091714157673	25.337354701312297	26.489789929130332	24.1147636553997
23	23.775586447808646	25.08209555446624	26.439131103824305	24.703186893900817
24	23.651054527340612	25.770619184314832	26.548000447368025	24.030325840976534
25	24.031654600934534	25.35275113763184	26.4675787815501	24.14801547988353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	394.0
1	394.0
2	813.5
3	1233.0
4	1233.0
5	1233.0
6	3147.0
7	5061.0
8	5061.0
9	5061.0
10	6410.5
11	7760.0
12	7760.0
13	7760.0
14	10348.5
15	12937.0
16	12937.0
17	12937.0
18	22014.0
19	31091.0
20	31091.0
21	31091.0
22	44781.5
23	58472.0
24	58472.0
25	58472.0
26	79151.0
27	99830.0
28	99830.0
29	99830.0
30	120558.5
31	141287.0
32	141287.0
33	141287.0
34	163785.5
35	186284.0
36	186284.0
37	186284.0
38	208201.0
39	230118.0
40	230118.0
41	230118.0
42	252575.5
43	275033.0
44	275033.0
45	275033.0
46	291767.0
47	308501.0
48	308501.0
49	308501.0
50	310981.0
51	313461.0
52	313461.0
53	313461.0
54	293487.0
55	273513.0
56	273513.0
57	273513.0
58	244417.5
59	215322.0
60	215322.0
61	215322.0
62	184741.0
63	154160.0
64	154160.0
65	154160.0
66	125105.0
67	96050.0
68	96050.0
69	96050.0
70	73784.5
71	51519.0
72	51519.0
73	51519.0
74	39939.5
75	28360.0
76	28360.0
77	28360.0
78	22667.5
79	16975.0
80	16975.0
81	16975.0
82	12741.0
83	8507.0
84	8507.0
85	8507.0
86	6186.0
87	3865.0
88	3865.0
89	3865.0
90	2735.5
91	1606.0
92	1606.0
93	1606.0
94	885.5
95	165.0
96	165.0
97	165.0
98	95.5
99	26.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3372158966976399
2	0.01050949225271958
3	0.0
4	0.0
5	0.0502472705063989
6	0.0031330184451503654
7	0.0037278953651156245
8	3.9658461331017273E-4
9	0.009676664564768216
10	0.0162996276070481
11	0.006186719967638695
12	0.009835298410092286
13	0.017211772217661497
14	0.0036089199811225724
15	0.021494886041411365
16	0.010668126098043648
17	0.006385012274293782
18	2.3795076798610368E-4
19	0.004679698437060039
20	0.004759015359722073
21	0.002815750754502227
22	0.0
23	0.0030933599838193476
24	0.004600381514398005
25	0.0025381415251851057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2521530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.64789821173392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.28292871179212	57.29940819693758
2	7.568723715961006	9.937416081962892
3	1.9333106549701613	3.807533432674254
4	0.9031422686566509	2.371575668939451
5	0.5296831187202824	1.738629173111144
6	0.3217785545393254	1.2674451477069903
7	0.2418109852149149	1.111206806070748
8	0.16825247981016214	0.8836337334759472
9	0.1311310789920926	0.774763175346096
>10	0.8107188415015164	9.892842802339603
>50	0.06378908304607919	2.9043273132047456
>100	0.040741657311731724	5.223445457032207
>500	0.003263604123279747	1.4401880412976693
>1k	6.043711339366253E-4	0.7391978676349075
>5k	1.2087422678732507E-4	0.6083871022657759
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9096	0.36073336426693314	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6238	0.2473894817828858	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3382	0.13412491622150044	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3216	0.12754161164055158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2879	0.11417671017199874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.965846133101728E-5
2	0.0	0.0	0.0	0.0	3.965846133101728E-5
3	0.0	3.965846133101728E-5	0.0	0.0	3.965846133101728E-5
4	0.0	3.965846133101728E-5	0.0	0.0	3.965846133101728E-5
5	0.0	7.931692266203455E-5	0.0	3.965846133101728E-5	3.965846133101728E-5
6	3.965846133101728E-5	7.931692266203455E-5	0.0	3.965846133101728E-5	3.965846133101728E-5
7	7.931692266203455E-5	7.931692266203455E-5	0.0	7.931692266203455E-5	3.965846133101728E-5
8	7.931692266203455E-5	7.931692266203455E-5	0.0	7.931692266203455E-5	3.965846133101728E-5
9	7.931692266203455E-5	7.931692266203455E-5	0.0	1.586338453240691E-4	3.965846133101728E-5
10	7.931692266203455E-5	7.931692266203455E-5	0.0	1.586338453240691E-4	3.965846133101728E-5
11	7.931692266203455E-5	7.931692266203455E-5	0.0	1.586338453240691E-4	7.931692266203455E-5
12	7.931692266203455E-5	7.931692266203455E-5	0.0	1.586338453240691E-4	1.586338453240691E-4
13	7.931692266203455E-5	7.931692266203455E-5	0.0	1.586338453240691E-4	1.586338453240691E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	14.946425	1
GTATCAA	3700	0.0	12.93282	1
TATAACG	75	1.4770187E-5	12.668691	2
CGTCGTA	205	0.0	12.046403	10
AACCGCG	95	1.0438471E-6	11.996918	7
ATACCGT	215	0.0	11.926932	6
TATACTG	345	0.0	11.567295	5
GCTCGTA	145	1.2223609E-9	11.135733	9
ACGAACG	70	0.0014949123	10.85457	15
CGAACGT	150	2.1282176E-9	10.7686	4
ACCGTCC	150	2.1445885E-9	10.763902	8
TACCGTC	230	0.0	10.736373	7
TAGATCG	100	2.3952447E-5	10.451877	5
ACTGCGT	110	6.0674447E-6	10.360975	8
ATTACCG	260	0.0	10.228345	15
ACCGTCG	205	3.6379788E-12	10.192504	8
TACTCCG	75	0.0026446048	10.135154	5
ACTATAC	235	0.0	10.107196	3
ACGGAAT	180	3.9835868E-10	10.023214	18
ATAAGAC	200	2.5465852E-11	9.975801	3
>>END_MODULE