FastQCFastQC Report
Thu 2 Feb 2017
SRR4050242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483759
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN84060.3384386327336912No Hit
GTATCAACGCAGAGTACTTTTTTTT54410.21906312166357525No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN38530.1551277720584002No Hit
TATCAACGCAGAGTACTTTTTTTTT31000.12481082101765911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.015.1538751
GCGTTAT602.5551948E-514.2584191
GTATCAA32800.012.75143051
ACGTTCC1457.2759576E-1212.4480516
GAACCGT853.9494516E-612.2931466
CGTTATT802.8661083E-511.8757492
TCTATAC2150.011.0463113
CGAACGT951.361254E-510.9998014
TATCACG700.001491155710.8578272
GTATTAT2950.010.6333971
AACCGCG1553.6689016E-910.4187437
CGTTCCC1606.177288E-910.09377217
GTATTAG3150.09.95826151
GTAGAAC1951.6370905E-109.7434133
GTTCTAG2650.09.6849641
GTCCTAT3700.09.5056131
ATTCGAA1106.795009E-59.5056121
CGCGCTA1304.241472E-69.49982716
GGAACCG900.0011141869.4998275
CGGAATT900.00111439289.49963515
Produced by FastQC (version 0.11.5)