FastQCFastQC Report
Thu 2 Feb 2017
SRR4050242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483759
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88260.3553484859038256No Hit
TATCAACGCAGAGTACTTTTTTTTT61470.24748777961146792No Hit
GGTATCAACGCAGAGTACTTTTTTT34340.13825818044343272No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32990.1328228704958895No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27510.11075953826438073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11150.015.9177641
CGCGAAC400.005305029514.23995812
CGTCGTA1950.013.14431310
CCGTCGT1950.012.6574879
GTATCAA34300.012.57442951
ATCACGC802.8297743E-511.890573
ACGCTTA658.075348E-411.68383417
CGCTTAC658.075348E-411.68383418
TACCGTC2450.011.2365237
CGCGCTA1105.018974E-711.21913516
ACGCGCT600.00590824911.07552315
CGTCTTA700.001500744610.84949315
GTCGTAG2400.010.67996911
CTCGACG803.7970365E-410.67996913
TCGACGC803.7970365E-410.67996914
ACCGTCG2600.010.5884748
CGAACGT1002.3654997E-510.4637024
TCTATAC2250.010.146623
ACCGCGC856.640008E-410.0515338
ACCGTCC1701.5807018E-910.0515338
Produced by FastQC (version 0.11.5)