##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2483759
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.72883762071924	31.0	31.0	34.0	27.0	34.0
2	30.90124484702421	31.0	31.0	34.0	27.0	34.0
3	31.080559748349177	31.0	31.0	34.0	27.0	34.0
4	34.66926501323196	37.0	35.0	37.0	32.0	37.0
5	34.543921531839445	37.0	35.0	37.0	32.0	37.0
6	34.56158830224672	37.0	35.0	37.0	31.0	37.0
7	34.46069365022935	37.0	35.0	37.0	31.0	37.0
8	34.442445100349914	37.0	35.0	37.0	31.0	37.0
9	36.064470023057794	39.0	37.0	39.0	31.0	39.0
10	35.949750358227185	39.0	37.0	39.0	31.0	39.0
11	35.95192488482176	39.0	37.0	39.0	31.0	39.0
12	35.86016920321175	39.0	37.0	39.0	30.0	39.0
13	35.85790811427357	39.0	37.0	39.0	30.0	39.0
14	37.03921113119268	40.0	37.0	41.0	31.0	41.0
15	37.01797920007537	40.0	37.0	41.0	31.0	41.0
16	36.982389998385514	40.0	37.0	41.0	31.0	41.0
17	36.972697834210166	40.0	37.0	41.0	31.0	41.0
18	36.94695177752753	40.0	37.0	41.0	31.0	41.0
19	36.985691043293656	40.0	37.0	41.0	31.0	41.0
20	36.958499999396075	40.0	37.0	41.0	31.0	41.0
21	36.891667025665534	40.0	37.0	41.0	31.0	41.0
22	36.82972140211671	40.0	37.0	41.0	31.0	41.0
23	36.74695089177332	40.0	37.0	41.0	30.0	41.0
24	36.6867304758634	40.0	37.0	41.0	30.0	41.0
25	36.40346386263724	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8911191039041064
1101	2	0.9787997125298453
1101	3	0.9470185677718561
1101	4	0.8156262846776698
1101	5	0.9510925427749584
1101	6	0.8903746545854716
1101	7	0.9944134575820485
1101	8	0.9426310545943508
1101	9	1.0220118693113847
1101	10	0.9404549637304171
1101	11	1.009083732985225
1101	12	1.1056379763271025
1101	13	1.101817224571839
1101	14	1.2764408864450587
1101	15	1.4139153360157408
1101	16	1.3280392024784717
1101	17	1.2343633619921732
1101	18	1.30439955542343
1101	19	1.2864207453644099
1101	20	1.3658211968240366
1101	21	1.328869643359866
1101	22	1.3716251612142472
1101	23	1.5065873429493521
1101	24	1.6101015731358004
1101	25	1.539143267213575
1102	1	0.6168164660806212
1102	2	0.7331606275179716
1102	3	0.7276878995607134
1102	4	0.7014865034420552
1102	5	0.6881283550727559
1102	6	0.6858806317214388
1102	7	0.7085151546318684
1102	8	0.7037039293331304
1102	9	0.845586656264075
1102	10	0.7821919583634127
1102	11	0.893233098414818
1102	12	0.9420414061451154
1102	13	0.9193752082338804
1102	14	1.114067777402262
1102	15	1.061898902004728
1102	16	1.181916354030406
1102	17	1.1138877490342338
1102	18	1.1579631038947582
1102	19	1.0402760354196943
1102	20	1.1405689802568304
1102	21	1.0752354585962465
1102	22	1.1029535931134617
1102	23	1.148273499374497
1102	24	1.1558236749132504
1102	25	1.1353939760668226
1103	1	0.6172704885914975
1103	2	0.6776192126063556
1103	3	0.6472066743537006
1103	4	0.6299704371542632
1103	5	0.6831157885733319
1103	6	0.6692085184092562
1103	7	0.763528683292634
1103	8	0.7547519247140855
1103	9	0.7343009742584812
1103	10	0.7890356565553276
1103	11	0.8230764946813451
1103	12	0.8707503763711344
1103	13	0.8236622546598866
1103	14	0.9071914917719326
1103	15	1.0123128036479727
1103	16	1.054181326374362
1103	17	0.9959747501000038
1103	18	0.9157478505030596
1103	19	0.9765514592727698
1103	20	1.0615259551745027
1103	21	1.0969952626212063
1103	22	1.0383962642212552
1103	23	1.164265041093934
1103	24	1.1448306664229548
1103	25	1.0556233217189614
1104	1	0.5085184209539477
1104	2	0.5937594528201977
1104	3	0.569338459873812
1104	4	0.4965339887460729
1104	5	0.48670925215407124
1104	6	0.43951583129377525
1104	7	0.45148382704191903
1104	8	0.5532965942361017
1104	9	0.6486113129831494
1104	10	0.7701747453749519
1104	11	0.5891380861189504
1104	12	0.6922544432387028
1104	13	0.5650829756991413
1104	14	0.6996810606175501
1104	15	0.7761317416369451
1104	16	0.846100169587082
1104	17	0.8217455379360317
1104	18	0.7819706371316144
1104	19	0.825526797959661
1104	20	0.7782649999269466
1104	21	0.7973792595540061
1104	22	0.7929505155704106
1104	23	0.8812392168527765
1104	24	0.8113234241731888
1104	25	0.7734079676483319
1105	1	0.4947552512875788
1105	2	0.4340095104529951
1105	3	0.4602131654122097
1105	4	0.5086597763306671
1105	5	0.39095820140515514
1105	6	0.41807849226484706
1105	7	0.4857564858908816
1105	8	0.5369012469965995
1105	9	0.6299975992784539
1105	10	0.6068985919175205
1105	11	0.6792749392003543
1105	12	0.6922269627002677
1105	13	0.6359869632779436
1105	14	0.6538984072494287
1105	15	0.734841240285057
1105	16	0.7162072215512509
1105	17	0.7491221083342978
1105	18	0.7950306103533507
1105	19	0.7718586804223406
1105	20	0.7897134685739431
1105	21	0.8337737213094911
1105	22	0.8228182731126736
1105	23	0.8189805303309114
1105	24	0.884282421683146
1105	25	0.7769882237974883
1106	1	0.4792614229786274
1106	2	0.5402067612250292
1106	3	0.5406088287349995
1106	4	0.538655609401907
1106	5	0.47937475961649767
1106	6	0.48584815471998866
1106	7	0.585515152497301
1106	8	0.6936399327218723
1106	9	0.6747896470891632
1106	10	0.7010145547984834
1106	11	0.7023847822184734
1106	12	0.7413657137497509
1106	13	0.7078503157744223
1106	14	0.750075538045806
1106	15	0.761272075321628
1106	16	0.6744879514695938
1106	17	0.7798637655434248
1106	18	0.8162370587863563
1106	19	0.8424807399837277
1106	20	0.7390388454654158
1106	21	0.806371661116124
1106	22	0.834229411954091
1106	23	0.8251910556208557
1106	24	0.8189729748038133
1106	25	0.8622533021880159
1107	1	0.37770718342152776
1107	2	0.41018416883772346
1107	3	0.32726343748476694
1107	4	0.2322665341339274
1107	5	0.16158641495455583
1107	6	0.2019808047713667
1107	7	0.2660101753652029
1107	8	0.2777986823288572
1107	9	0.2630091919352111
1107	10	0.32341375612355705
1107	11	0.26984850312307884
1107	12	0.39449185836048173
1107	13	0.35152331284746197
1107	14	0.3920643164037969
1107	15	0.41054552100104047
1107	16	0.4576665091223191
1107	17	0.3815927152405223
1107	18	0.31938929646993586
1107	19	0.4197386011296871
1107	20	0.41675665779625604
1107	21	0.3714250951914053
1107	22	0.3766859207981881
1107	23	0.4044592178581823
1107	24	0.38279987238607305
1107	25	0.34793193277169365
1108	1	0.2361807440701682
1108	2	0.2171516268898941
1108	3	0.18017961090924572
1108	4	0.20790555097795504
1108	5	0.1848052742332129
1108	6	0.20456146884020399
1108	7	0.25921667349742705
1108	8	0.28730326307037757
1108	9	0.33824199701531654
1108	10	0.26492910079648624
1108	11	0.31419435146300856
1108	12	0.40329977703020603
1108	13	0.3939221497902281
1108	14	0.46570074379174287
1108	15	0.43686293556416445
1108	16	0.5218894158652674
1108	17	0.5205598425456728
1108	18	0.44663165134683425
1108	19	0.4572111915172954
1108	20	0.48272059171802084
1108	21	0.537106742813954
1108	22	0.5670502972510931
1108	23	0.5462693041768745
1108	24	0.44253906370961005
1108	25	0.420258141437472
1109	1	0.3814887210062743
1109	2	0.4219277229418381
1109	3	0.3588146551169089
1109	4	0.26741457909580646
1109	5	0.22120969623691167
1109	6	0.26739550081946817
1109	7	0.29146533956462406
1109	8	0.3701673402171721
1109	9	0.4309577600719763
1109	10	0.4555359149989755
1109	11	0.3713893269595161
1109	12	0.5215491431402342
1109	13	0.46882314002753844
1109	14	0.5126024157127205
1109	15	0.4677308729242995
1109	16	0.5337919398908255
1109	17	0.45842685117168713
1109	18	0.5180122722936034
1109	19	0.38076104721827875
1109	20	0.5490819774515998
1109	21	0.5695074706349885
1109	22	0.5362895215206223
1109	23	0.4988324227758625
1109	24	0.47061760580058376
1109	25	0.5233324629167768
1110	1	-0.035806119894743915
1110	2	-0.04726855098263627
1110	3	-0.010897446678171718
1110	4	-0.111160739029593
1110	5	-0.17800946340850032
1110	6	-0.20183503543329095
1110	7	-0.14269741974535322
1110	8	-0.11518851160551691
1110	9	-0.22041705528688738
1110	10	-0.1493794355967637
1110	11	-0.1045860294034
1110	12	-0.17801655187709287
1110	13	-0.06725042239015266
1110	14	-0.031498248648340166
1110	15	-0.0695490641794052
1110	16	-0.17192892245979863
1110	17	-0.14819417024952486
1110	18	-0.10943601714546247
1110	19	-0.14577553617768046
1110	20	-0.08347815041061324
1110	21	-0.13094929731061455
1110	22	-0.18295751491417178
1110	23	0.006812264159584913
1110	24	-0.020375250851003557
1110	25	-0.040134051809396
1111	1	-0.19698145031681946
1111	2	-0.26783571349933055
1111	3	-0.21367793164830928
1111	4	-0.248308328162139
1111	5	-0.2941694849100003
1111	6	-0.32129558515006096
1111	7	-0.23366878861346407
1111	8	-0.2394092942067374
1111	9	-0.27389900238303255
1111	10	-0.3074521823282623
1111	11	-0.22668723171965155
1111	12	-0.268178345799285
1111	13	-0.1388340028010333
1111	14	-0.2950612016658738
1111	15	-0.37317768393099726
1111	16	-0.33935887612746285
1111	17	-0.32995573123883304
1111	18	-0.3167832094204215
1111	19	-0.4185470258706445
1111	20	-0.36571544412760204
1111	21	-0.3522395929440947
1111	22	-0.15243198733450924
1111	23	-0.26641389260521464
1111	24	-0.3039606860081321
1111	25	-0.30844597848154365
1112	1	-0.25386012972191097
1112	2	-0.31504462296054925
1112	3	-0.21709287269069222
1112	4	-0.16898735449294122
1112	5	-0.23307884156380254
1112	6	-0.24260560730636627
1112	7	-0.1450613944252268
1112	8	-0.16700592161479477
1112	9	-0.19385408561151252
1112	10	-0.26918201247872986
1112	11	-0.21967209409378086
1112	12	-0.18062950490992336
1112	13	-0.13842509287507454
1112	14	-0.17964124844159102
1112	15	-0.1293554827768233
1112	16	-0.24174227667329262
1112	17	-0.21570894692415266
1112	18	-0.13428018572932388
1112	19	-0.10557594293030093
1112	20	-0.22459022439443288
1112	21	-0.23970268997059208
1112	22	-0.2712486406980972
1112	23	-0.2769025280578603
1112	24	-0.22624984963368178
1112	25	-0.26864397941798757
1113	1	-0.42925146700900285
1113	2	-0.47216938194000946
1113	3	-0.41459295038280786
1113	4	-0.4627717892484995
1113	5	-0.5218384022435885
1113	6	-0.5703127435861788
1113	7	-0.6068453565887424
1113	8	-0.5651500459490606
1113	9	-0.6935002855151424
1113	10	-0.5065520041719722
1113	11	-0.5692167343036942
1113	12	-0.6040097082868812
1113	13	-0.6250149857327472
1113	14	-0.8346069505221436
1113	15	-0.7417451774706052
1113	16	-0.7065810088387536
1113	17	-0.6482960159883859
1113	18	-0.7040435901390438
1113	19	-0.8166043317694047
1113	20	-0.7236636024280259
1113	21	-0.8959494300514095
1113	22	-0.7804477511723675
1113	23	-0.8501213418126454
1113	24	-0.9086566309935122
1113	25	-0.8413231759331268
1114	1	-0.7165132827735334
1114	2	-1.30284029589901
1114	3	-0.901403705871676
1114	4	-0.8135260191427491
1114	5	-1.0239086499873622
1114	6	-0.9663571965418853
1114	7	-0.8651024995898311
1114	8	-0.9073611924505798
1114	9	-0.8836590197338907
1114	10	-0.9647800700843945
1114	11	-0.9982636828952138
1114	12	-0.9142930820109072
1114	13	-0.8610138166699102
1114	14	-0.9741127540643362
1114	15	-1.0339187070111748
1114	16	-1.1301375808005858
1114	17	-1.1190615556078285
1114	18	-1.236707009937959
1114	19	-1.0408339730045242
1114	20	-1.1022217187641772
1114	21	-1.1528756263435724
1114	22	-1.135054307767966
1114	23	-1.208778815202983
1114	24	-1.334103490537828
1114	25	-1.3314380453815957
1115	1	-0.8659564634138732
1115	2	-1.2408765079780686
1115	3	-1.0634952562047175
1115	4	-0.9453720823475038
1115	5	-1.0986318223356903
1115	6	-1.0474796727978273
1115	7	-1.1821035197252314
1115	8	-1.247192133648234
1115	9	-1.630197433014196
1115	10	-1.3684379919540248
1115	11	-1.3838026907998966
1115	12	-1.3023236206096627
1115	13	-1.63201829544618
1115	14	-1.6125759500885337
1115	15	-1.648880711058986
1115	16	-1.675160301998126
1115	17	-1.6540923506042517
1115	18	-1.5653842161853362
1115	19	-1.5757378784319727
1115	20	-1.7508458452391409
1115	21	-1.605738442681016
1115	22	-1.7789390597441397
1115	23	-1.837440473932233
1115	24	-1.8122687742936705
1115	25	-1.6984489395548223
1116	1	-0.7021090938044807
1116	2	-0.714148126853658
1116	3	-0.7729014427027998
1116	4	-0.7554488491120068
1116	5	-0.9110432018796004
1116	6	-1.0048334772683631
1116	7	-1.056264729930696
1116	8	-1.058736500322425
1116	9	-1.13392062109277
1116	10	-1.1630801233606647
1116	11	-1.1148096901225486
1116	12	-1.3247462893836541
1116	13	-1.2475387289526125
1116	14	-1.4125195039381566
1116	15	-1.481423045671761
1116	16	-1.4608523318016964
1116	17	-1.6728866608322974
1116	18	-1.6088048528979115
1116	19	-1.544421554205286
1116	20	-1.4975516479034
1116	21	-1.404964001497902
1116	22	-1.5225674182900875
1116	23	-1.7675511083869608
1116	24	-1.7564999777255963
1116	25	-1.9347551034744441
1201	1	0.7716189000879154
1201	2	0.919531333423091
1201	3	0.8822538134243771
1201	4	0.7653218567529763
1201	5	0.9155818931990822
1201	6	0.8179556301088056
1201	7	0.9566176180249997
1201	8	0.8859247818090665
1201	9	1.0272914647902667
1201	10	1.0770859901717884
1201	11	0.9849491595660211
1201	12	1.058814267967179
1201	13	1.0944072817988157
1201	14	1.2819684102728246
1201	15	1.3178208646918819
1201	16	1.2086933012004124
1201	17	1.3336436315768978
1201	18	1.252714506889248
1201	19	1.26497017187031
1201	20	1.358511541698313
1201	21	1.2839895708214684
1201	22	1.365136172329791
1201	23	1.3640049558368688
1201	24	1.3705266271146783
1201	25	1.3780600597949544
1202	1	0.5159521171795483
1202	2	0.664843797025128
1202	3	0.6049358258498394
1202	4	0.5911126335129353
1202	5	0.6893095420235227
1202	6	0.6314930481731835
1202	7	0.7442360341872103
1202	8	0.7403683844766249
1202	9	0.8759715420174032
1202	10	0.9190806629873265
1202	11	0.9300990122705173
1202	12	0.8993676248200444
1202	13	0.8508466142353583
1202	14	1.005379685810503
1202	15	1.0453860715922616
1202	16	0.9980525614164364
1202	17	1.0251394327945462
1202	18	0.9110205790520496
1202	19	1.0365981687592551
1202	20	1.0405936774118274
1202	21	1.0079107220941097
1202	22	0.9513613221894488
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2211	4	-0.3367851110432696
2211	5	-0.28602449652763084
2211	6	-0.384219552212123
2211	7	-0.3686876803852783
2211	8	-0.32075870511565796
2211	9	-0.4260875458932958
2211	10	-0.4357515601121449
2211	11	-0.39319137569042084
2211	12	-0.5083510923898942
2211	13	-0.5333653646696277
2211	14	-0.6712685472144244
2211	15	-0.6563008116981806
2211	16	-0.5235636747711467
2211	17	-0.48881581948317177
2211	18	-0.6176365848330505
2211	19	-0.5785111229126798
2211	20	-0.6013766943256869
2211	21	-0.580762015825762
2211	22	-0.5587740477308998
2211	23	-0.5436730774047405
2211	24	-0.556341488378628
2211	25	-0.4902788049158957
2212	1	-0.4701970331256824
2212	2	-0.5442658481360745
2212	3	-0.5760611847682
2212	4	-0.48317001269133186
2212	5	-0.498655189421477
2212	6	-0.5268342961426598
2212	7	-0.5806937055492014
2212	8	-0.6172366423830695
2212	9	-0.6793039201622975
2212	10	-0.7995562739168633
2212	11	-0.8265911630375768
2212	12	-0.8346403002580445
2212	13	-0.800497209433054
2212	14	-0.8523778862184983
2212	15	-0.8865506787901509
2212	16	-0.8391404956111259
2212	17	-0.8390506236653437
2212	18	-0.8346709637095344
2212	19	-0.9666247149736407
2212	20	-0.8896719273577602
2212	21	-0.986667562520779
2212	22	-1.0409998141671934
2212	23	-0.883204355093909
2212	24	-0.8417836290839276
2212	25	-0.8241304795570485
2213	1	-0.7282688655994285
2213	2	-0.616222599518661
2213	3	-0.6114274931010151
2213	4	-0.6425871505773699
2213	5	-0.6651437248095391
2213	6	-0.6206017726852551
2213	7	-0.671835817026242
2213	8	-0.6738078223992261
2213	9	-0.8049952145138803
2213	10	-0.7755116582454491
2213	11	-0.8665371022658093
2213	12	-0.8310238210434804
2213	13	-0.9091842162061852
2213	14	-1.012761282726295
2213	15	-1.0761828169868792
2213	16	-1.0140985519088943
2213	17	-1.0070304906882726
2213	18	-0.9737966705385901
2213	19	-1.0771821001721733
2213	20	-1.0909364523064937
2213	21	-1.1058436017925146
2213	22	-1.021485118336706
2213	23	-1.0756420609287431
2213	24	-1.146043245066032
2213	25	-1.051651762102253
2214	1	-1.235467616712402
2214	2	-1.7453663038964358
2214	3	-1.2686115352744878
2214	4	-1.268765532750919
2214	5	-1.2607628142673448
2214	6	-1.4299950975723874
2214	7	-1.3281785078600237
2214	8	-1.4424413030834486
2214	9	-1.6321765502424839
2214	10	-1.6361219027276377
2214	11	-1.5327300757073488
2214	12	-1.7974493918483958
2214	13	-1.7023172987684418
2214	14	-2.0099893340467645
2214	15	-1.960883558709675
2214	16	-2.017063481874729
2214	17	-1.8619328821657177
2214	18	-1.9689105569541994
2214	19	-2.008058713742841
2214	20	-2.0127252946933467
2214	21	-2.1225443278257714
2214	22	-2.1258730891698647
2214	23	-2.102594721677839
2214	24	-2.0256052716833253
2214	25	-2.0443435764793563
2215	1	-1.006317490066298
2215	2	-1.1910712651479258
2215	3	-1.170687849406022
2215	4	-0.9433201471022059
2215	5	-1.155856738105264
2215	6	-1.1213383848256555
2215	7	-1.090016522710961
2215	8	-1.1459126530006856
2215	9	-1.3889716407003974
2215	10	-1.3293608286702678
2215	11	-1.425139694662171
2215	12	-1.4818379603253362
2215	13	-1.6712277621238059
2215	14	-1.7592396036344269
2215	15	-1.933176599518731
2215	16	-1.7401859135246838
2215	17	-1.7333461923332365
2215	18	-1.7380560325493164
2215	19	-1.8233917530313661
2215	20	-1.6634510990260907
2215	21	-1.5914483065427376
2215	22	-1.6915966322644778
2215	23	-1.6910351270124622
2215	24	-1.7373187647064725
2215	25	-1.7869141654019174
2216	1	-1.1914937244194768
2216	2	-1.1473656422946519
2216	3	-1.3860712520258147
2216	4	-1.3306064261156578
2216	5	-1.3225313687327187
2216	6	-1.2681435002261523
2216	7	-1.4175765514028171
2216	8	-1.4937684173020003
2216	9	-1.6707167577800632
2216	10	-1.5625271347989838
2216	11	-1.5832986105052456
2216	12	-1.8278235166700867
2216	13	-1.699557379392047
2216	14	-1.9665192577123705
2216	15	-1.9811138661848062
2216	16	-1.9405443187563876
2216	17	-2.0571542506197105
2216	18	-2.075239873429922
2216	19	-1.9551669036919535
2216	20	-2.1586320388652496
2216	21	-2.0992924299630715
2216	22	-2.137776482255852
2216	23	-2.163883555021094
2216	24	-2.3375047704345846
2216	25	-2.16822704188467
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34320.0
3	2.0
4	663.0
5	5027.0
6	5946.0
7	4868.0
8	4091.0
9	3433.0
10	3079.0
11	2788.0
12	2771.0
13	2607.0
14	2508.0
15	2582.0
16	2674.0
17	2882.0
18	3270.0
19	4033.0
20	5203.0
21	6882.0
22	8959.0
23	11137.0
24	13182.0
25	15429.0
26	17032.0
27	19545.0
28	22438.0
29	27432.0
30	35698.0
31	46383.0
32	60342.0
33	79532.0
34	107890.0
35	149244.0
36	218016.0
37	384619.0
38	1167252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63275782293995	18.304110218437255	12.949752312665218	26.11337964595757
2	15.943260812233632	21.06516833122286	37.73059354295803	25.260977313585474
3	18.292354451458454	25.07344714201338	29.299903895667818	27.334294510860353
4	12.934084818264981	17.32413545411207	37.29743245332612	32.44434727429683
5	14.663872019461973	36.35576821474635	34.066276211916744	14.914083553874931
6	31.738321687109995	37.49039327553455	17.06130097203134	13.709984065324116
7	28.08063450471695	31.1849936848276	22.177750059286524	18.55662175116892
8	25.978762740825555	34.355941101915334	20.220771189347712	19.4445249679114
9	26.596665130014834	15.39781500188223	19.252985902393753	38.752533965709176
10	15.197918268777816	27.709966815664682	32.697884045670534	24.394230869886968
11	35.16531211574868	22.55051483134613	23.371136751799515	18.913036301105677
12	23.954034909858677	24.451637086601462	29.837730718267945	21.756597285271916
13	29.055491290845616	21.017123581372747	25.292724299831175	24.634660827950462
14	22.869450129633037	20.375398270781048	26.607648973303927	30.147502626281984
15	24.17704975588702	27.65083831047886	23.44410686375009	24.728005069884034
16	24.32754852746736	27.011798298378096	24.58667304412525	24.073980130029295
17	23.78567597732972	26.159806474784226	25.873730272619948	24.180787275266105
18	24.3753490190426	25.667912230996155	26.586604835355615	23.370133914605635
19	25.42347427669563	25.50846706982212	26.113119730728656	22.95493892275359
20	24.60576485603965	25.560803166282152	26.204930606723114	23.628501370955078
21	24.76800479290341	25.353589362846623	26.089395056996256	23.789010787253712
22	24.21635158340161	25.34767195373097	26.383531254340742	24.052445208526674
23	23.807290211352836	25.02401645277999	26.45264309819891	24.716050237668266
24	23.714731825787823	25.74308970062214	26.476637124529528	24.06554134906051
25	24.13818233942184	25.25281971083771	26.385898144136625	24.22309980560383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	430.0
1	430.0
2	806.5
3	1183.0
4	1183.0
5	1183.0
6	3143.5
7	5104.0
8	5104.0
9	5104.0
10	6252.0
11	7400.0
12	7400.0
13	7400.0
14	10000.5
15	12601.0
16	12601.0
17	12601.0
18	21433.0
19	30265.0
20	30265.0
21	30265.0
22	43604.5
23	56944.0
24	56944.0
25	56944.0
26	77411.5
27	97879.0
28	97879.0
29	97879.0
30	117934.0
31	137989.0
32	137989.0
33	137989.0
34	160312.0
35	182635.0
36	182635.0
37	182635.0
38	204272.5
39	225910.0
40	225910.0
41	225910.0
42	248486.0
43	271062.0
44	271062.0
45	271062.0
46	286782.0
47	302502.0
48	302502.0
49	302502.0
50	305727.5
51	308953.0
52	308953.0
53	308953.0
54	289627.0
55	270301.0
56	270301.0
57	270301.0
58	242100.0
59	213899.0
60	213899.0
61	213899.0
62	183184.5
63	152470.0
64	152470.0
65	152470.0
66	123978.5
67	95487.0
68	95487.0
69	95487.0
70	73446.5
71	51406.0
72	51406.0
73	51406.0
74	39871.0
75	28336.0
76	28336.0
77	28336.0
78	22578.0
79	16820.0
80	16820.0
81	16820.0
82	12654.5
83	8489.0
84	8489.0
85	8489.0
86	6170.5
87	3852.0
88	3852.0
89	3852.0
90	2748.0
91	1644.0
92	1644.0
93	1644.0
94	908.0
95	172.0
96	172.0
97	172.0
98	99.0
99	26.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4544321731697802
2	0.0045898172890364965
3	0.0
4	0.005757402388879114
5	0.17139344034586285
6	0.04521372645252619
7	0.002335170199685235
8	4.4287710683685495E-4
9	1.6104622066794724E-4
10	0.0022949086445182483
11	0.0013286313205105647
12	0.001932554648015367
13	0.004348247958034576
14	0.00845492658506723
15	0.0029390935271900375
16	4.4287710683685495E-4
17	0.006240541050882956
18	4.8313866200384175E-4
19	7.649695481727495E-4
20	0.003583278409861827
21	0.0035027552995278524
22	9.662773240076835E-4
23	0.0018117699825144066
24	0.003019616637524011
25	0.006884725933554745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2483759.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.57122846516559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.38540623566524	57.29968436800112
2	7.486397092413559	9.817845082552015
3	1.9317725591968058	3.8000609946549404
4	0.8623092178167273	2.2617069891631543
5	0.5069630270585773	1.6621094235324947
6	0.33690446306117733	1.3254743710991037
7	0.2388095193123691	1.0961323485341408
8	0.17265170858037712	0.9056787702580078
9	0.14491048158838213	0.8551762465706078
>10	0.8250977196160499	10.04308263731844
>50	0.06382459504226252	2.887317339132691
>100	0.04120192964402267	5.342250890438855
>500	0.0028904622492488727	1.2684876989725262
>1k	7.379903615088545E-4	0.8311949533055769
>5k	1.2299839358480912E-4	0.6037978864663498
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8826	0.3553484859038256	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6147	0.24748777961146792	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3434	0.13825818044343272	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3299	0.1328228704958895	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2751	0.11075953826438073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.026155516698681E-5
7	0.0	0.0	0.0	4.026155516698681E-5	4.026155516698681E-5
8	0.0	0.0	0.0	4.026155516698681E-5	4.026155516698681E-5
9	0.0	0.0	0.0	8.052311033397362E-5	4.026155516698681E-5
10	0.0	0.0	0.0	8.052311033397362E-5	4.026155516698681E-5
11	0.0	0.0	0.0	8.052311033397362E-5	8.052311033397362E-5
12	0.0	0.0	0.0	8.052311033397362E-5	2.0130777583493407E-4
13	0.0	0.0	0.0	8.052311033397362E-5	2.4156933100192088E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1115	0.0	15.917764	1
CGCGAAC	40	0.0053050295	14.239958	12
CGTCGTA	195	0.0	13.144313	10
CCGTCGT	195	0.0	12.657487	9
GTATCAA	3430	0.0	12.5744295	1
ATCACGC	80	2.8297743E-5	11.89057	3
ACGCTTA	65	8.075348E-4	11.683834	17
CGCTTAC	65	8.075348E-4	11.683834	18
TACCGTC	245	0.0	11.236523	7
CGCGCTA	110	5.018974E-7	11.219135	16
ACGCGCT	60	0.005908249	11.075523	15
CGTCTTA	70	0.0015007446	10.849493	15
GTCGTAG	240	0.0	10.679969	11
CTCGACG	80	3.7970365E-4	10.679969	13
TCGACGC	80	3.7970365E-4	10.679969	14
ACCGTCG	260	0.0	10.588474	8
CGAACGT	100	2.3654997E-5	10.463702	4
TCTATAC	225	0.0	10.14662	3
ACCGCGC	85	6.640008E-4	10.051533	8
ACCGTCC	170	1.5807018E-9	10.051533	8
>>END_MODULE