FastQCFastQC Report
Thu 2 Feb 2017
SRR4050241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341734
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80930.24217965882383216No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN75760.22670864886313513No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN57500.17206635836365192No Hit
TATCAACGCAGAGTACTTTTTTTTT47080.14088494176975186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.015.0249361
GTATCAA45500.013.1135541
AGAACCG2450.012.0217525
AACCGCG1900.011.9997147
GATTACG1157.08842E-811.56459312
TCGACGC1252.2434506E-710.63974714
CGAGCCG3500.010.58546315
ACCGTCC1502.4499968E-810.1327878
ACTGTCG951.645996E-49.999468
CGATAAC1901.0004442E-109.9993110
GAACCGC2600.09.8655966
ACGCTTA1356.452701E-79.85146917
TAGAACC4350.09.8286854
CGCTTAC1451.6198283E-79.82720518
CGAACGA1752.577508E-99.77119616
ATCGCTC1856.493792E-109.75652516
GATAACG1856.493792E-109.7562311
GCCGCCT3900.09.74321218
CGCAGAT2054.1836756E-119.7311838
ATTACCG2850.09.66643715
Produced by FastQC (version 0.11.5)