##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050241_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3341734
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.700571619404776	31.0	30.0	34.0	25.0	34.0
2	29.810240432063114	31.0	30.0	34.0	25.0	34.0
3	29.925701746458575	31.0	31.0	34.0	26.0	34.0
4	33.30333802750309	37.0	35.0	37.0	27.0	37.0
5	33.39020670107196	37.0	35.0	37.0	28.0	37.0
6	33.45529955406385	37.0	35.0	37.0	28.0	37.0
7	33.34795887404563	37.0	35.0	37.0	27.0	37.0
8	33.288862608454174	37.0	35.0	37.0	27.0	37.0
9	34.74052542781681	39.0	35.0	39.0	27.0	39.0
10	34.63960536655521	39.0	35.0	39.0	27.0	39.0
11	34.66799362247264	39.0	35.0	39.0	27.0	39.0
12	34.55864649909299	39.0	35.0	39.0	27.0	39.0
13	34.54803165063407	39.0	35.0	39.0	27.0	39.0
14	35.384829552561634	40.0	36.0	41.0	25.0	41.0
15	35.51541804344691	40.0	36.0	41.0	26.0	41.0
16	35.53197202410485	40.0	36.0	41.0	26.0	41.0
17	35.519077520832	40.0	36.0	41.0	26.0	41.0
18	35.35265045033506	40.0	36.0	41.0	25.0	41.0
19	35.48998603718908	40.0	36.0	41.0	26.0	41.0
20	35.45100417926741	40.0	36.0	41.0	25.0	41.0
21	35.413660093831524	40.0	36.0	41.0	25.0	41.0
22	35.33661207026053	40.0	36.0	41.0	25.0	41.0
23	35.23287909809697	40.0	36.0	41.0	25.0	41.0
24	35.16602518333296	40.0	36.0	41.0	24.0	41.0
25	34.882181825363716	39.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.1897775996639552
1101	2	1.2304421334667595
1101	3	1.1500522621630367
1101	4	1.2522214674554277
1101	5	1.2172169532950718
1101	6	1.1745680070734963
1101	7	1.200213938138205
1101	8	1.1649292440336154
1101	9	1.3656351507431097
1101	10	1.38199208621851
1101	11	1.3966255550375877
1101	12	1.4297510686374153
1101	13	1.435649212402268
1101	14	1.8896460093561984
1101	15	1.676410900412428
1101	16	1.7248908737558608
1101	17	1.7173064987257334
1101	18	1.802163932042511
1101	19	1.7359419493911759
1101	20	1.6998731806805552
1101	21	1.6615312097798736
1101	22	1.7299592345168904
1101	23	1.6789297303370674
1101	24	1.7292485640351316
1101	25	1.855217918923799
1102	1	1.0143962059166363
1102	2	0.9839582992210794
1102	3	0.902264322473961
1102	4	1.0878497218631509
1102	5	1.0067831142475612
1102	6	1.0692344090541965
1102	7	1.0530467807484314
1102	8	0.9938203035036324
1102	9	1.1220148439416207
1102	10	1.2477673116115042
1102	11	1.2266664173985404
1102	12	1.251278353544926
1102	13	1.258092357621173
1102	14	1.5627699596889286
1102	15	1.5058802284614075
1102	16	1.4645133683157425
1102	17	1.4201218322417688
1102	18	1.5065609399198436
1102	19	1.448195504498564
1102	20	1.4305607669084566
1102	21	1.4731679060304685
1102	22	1.4847408568779414
1102	23	1.5074322518808714
1102	24	1.5581756371938624
1102	25	1.4454202045925797
1103	1	0.8567870607910635
1103	2	0.8791105328036082
1103	3	0.7983498202199542
1103	4	0.9598832352776228
1103	5	0.9422911215510581
1103	6	0.9419617096276269
1103	7	0.9457326796462553
1103	8	0.9517173222655444
1103	9	1.1720762586548048
1103	10	1.11876801082866
1103	11	1.0663602321252057
1103	12	1.155256846282235
1103	13	1.2106358122551768
1103	14	1.6183553570495022
1103	15	1.4126040744398267
1103	16	1.359037253223093
1103	17	1.3372082102049845
1103	18	1.3660000513599115
1103	19	1.3637357242437744
1103	20	1.256523081581797
1103	21	1.223823195868654
1103	22	1.3187788373739266
1103	23	1.2727042713041214
1103	24	1.314499988190299
1103	25	1.3371526508585347
1104	1	0.7434461608533063
1104	2	0.7601943989612501
1104	3	0.7294650067944453
1104	4	0.9185561726844966
1104	5	0.8650774958938285
1104	6	0.7604196090184203
1104	7	0.7842546843748295
1104	8	0.7632663570796865
1104	9	0.8681286879009562
1104	10	0.8742063912646643
1104	11	0.9012715686072212
1104	12	0.9907038362270768
1104	13	0.9740571600649162
1104	14	1.3681797607910866
1104	15	1.25169199904893
1104	16	1.135647702903512
1104	17	1.1543362511312196
1104	18	1.2724698087958757
1104	19	1.213605993590484
1104	20	1.1950663659984713
1104	21	1.2356050868318817
1104	22	1.2771160039357525
1104	23	1.1904252366785855
1104	24	1.2236662082941692
1104	25	1.2922050679004826
1105	1	0.6080597536860424
1105	2	0.7272136233371498
1105	3	0.6001370985257957
1105	4	0.6859985349469824
1105	5	0.7495667726686506
1105	6	0.6879463929517016
1105	7	0.6851604585511453
1105	8	0.7428300926528237
1105	9	0.8298314489786165
1105	10	0.8414430614989428
1105	11	0.7739426265162805
1105	12	0.8338805018713629
1105	13	0.8377240976988318
1105	14	1.1853546805197226
1105	15	1.042944790047386
1105	16	0.9838007000229609
1105	17	0.9794405449254953
1105	18	1.0820015216163483
1105	19	0.9647967552515482
1105	20	1.004755297021937
1105	21	0.9888131746681736
1105	22	0.9673229614310657
1105	23	0.8725907726468094
1105	24	0.9503042482357387
1105	25	0.8971623530230914
1106	1	0.5200272148928384
1106	2	0.45021495650713206
1106	3	0.44140602819427244
1106	4	0.6903384751086037
1106	5	0.530430176103593
1106	6	0.36948402511576717
1106	7	0.4833803314643319
1106	8	0.5429653020727443
1106	9	0.7398792128807372
1106	10	0.7433654246499088
1106	11	0.79075420194998
1106	12	0.7092530518331515
1106	13	0.7533997181807308
1106	14	1.0528767656986062
1106	15	0.825092448475786
1106	16	0.8503359695396142
1106	17	0.7995517057789598
1106	18	1.0342718065056289
1106	19	0.9493163750985758
1106	20	0.8726446765783038
1106	21	0.8076582230931848
1106	22	0.8860312700429276
1106	23	0.8512863822859487
1106	24	0.9413537104629626
1106	25	0.8899227702082868
1107	1	0.45810716644358607
1107	2	0.4852652002207627
1107	3	0.4422230081512879
1107	4	0.5000991390582854
1107	5	0.41446264773453834
1107	6	0.49166136846116615
1107	7	0.5083922741007569
1107	8	0.43039510670078585
1107	9	0.5832493706944035
1107	10	0.6014114788099505
1107	11	0.5577688497646207
1107	12	0.5374838069044117
1107	13	0.42541018894011273
1107	14	0.8029813997263133
1107	15	0.737157109813154
1107	16	0.5855432176895974
1107	17	0.5226415780831246
1107	18	0.6364598778546835
1107	19	0.5470434890797051
1107	20	0.5018508319938633
1107	21	0.5732887195437755
1107	22	0.6131668463954725
1107	23	0.7044800754348302
1107	24	0.667776248731407
1107	25	0.6472709820481555
1108	1	0.3727921052864147
1108	2	0.4323430666865882
1108	3	0.3806496963233421
1108	4	0.5635971543163194
1108	5	0.4208300867838659
1108	6	0.37664851975278424
1108	7	0.40182137499656534
1108	8	0.3841521344287102
1108	9	0.4580994189681604
1108	10	0.49532640209058343
1108	11	0.42006324864396305
1108	12	0.46977137211099773
1108	13	0.5258709624939826
1108	14	0.7565119237088354
1108	15	0.5711756356138764
1108	16	0.5390499113495721
1108	17	0.5153010289692546
1108	18	0.701269987587537
1108	19	0.5855693700053664
1108	20	0.5900259174610483
1108	21	0.616017066283149
1108	22	0.6190783859245386
1108	23	0.683406782385795
1108	24	0.62396408504069
1108	25	0.7060777013970565
1109	1	0.15088847747436063
1109	2	0.2237081788934816
1109	3	0.2104002552022557
1109	4	0.33351501288983343
1109	5	0.22090180990561237
1109	6	0.23505179819402855
1109	7	0.2579921381333605
1109	8	0.18539455261704063
1109	9	0.22645991368968055
1109	10	0.2910994259383699
1109	11	0.20025022006613824
1109	12	0.30830021252056383
1109	13	0.35310011085466897
1109	14	0.5934647606557064
1109	15	0.3792692921193037
1109	16	0.3996222479095337
1109	17	0.4010054114192627
1109	18	0.40914768338811314
1109	19	0.38483707708234505
1109	20	0.36523645036376706
1109	21	0.37894462079209745
1109	22	0.3611356146299798
1109	23	0.4970627687555478
1109	24	0.39680116167915713
1109	25	0.4028707545004053
1110	1	-0.08838560794988481
1110	2	-2.0326846653873076E-4
1110	3	-0.04817089974890365
1110	4	0.046014214830712774
1110	5	-0.12112238724691338
1110	6	-0.11947798537233467
1110	7	-0.14111722788847914
1110	8	-0.0881960749766506
1110	9	-0.11180542500661517
1110	10	-0.21218857763693677
1110	11	-0.21387861859822976
1110	12	-0.12933944782547258
1110	13	-0.14701959801336528
1110	14	-0.047177198100598616
1110	15	-0.22206825496618876
1110	16	-0.22116399993571179
1110	17	-0.3765914729579336
1110	18	-0.22463096693429918
1110	19	-0.24590096799203565
1110	20	-0.40404465798074085
1110	21	-0.317186388593079
1110	22	-0.28346471736261947
1110	23	-0.30165874053385977
1110	24	-0.35476303419647337
1110	25	-0.5091192857217735
1111	1	-0.33028394990339294
1111	2	-0.28553091326848445
1111	3	-0.36543367547053407
1111	4	-0.2809875952751071
1111	5	-0.34256425129075296
1111	6	-0.2559565731768174
1111	7	-0.3061491916446286
1111	8	-0.32198988983986254
1111	9	-0.5377325825927528
1111	10	-0.3932821006401497
1111	11	-0.35667901400462654
1111	12	-0.2963661794890271
1111	13	-0.41901101435595933
1111	14	-0.1311977326295377
1111	15	-0.33941175514745936
1111	16	-0.38810537754621066
1111	17	-0.39751201870222985
1111	18	-0.33022201603928636
1111	19	-0.46146435690656773
1111	20	-0.3766926289147179
1111	21	-0.460471652401651
1111	22	-0.5625849250813886
1111	23	-0.44114962871157104
1111	24	-0.5102374305887807
1111	25	-0.550151046036639
1112	1	-0.5385529703568253
1112	2	-0.6320294744697854
1112	3	-0.6442719327789348
1112	4	-0.5166755427182324
1112	5	-0.662360259884764
1112	6	-0.668789106315721
1112	7	-0.7156140826294504
1112	8	-0.7126759369828193
1112	9	-0.906737678499745
1112	10	-0.7894054882936246
1112	11	-0.9103464382059201
1112	12	-0.7900053706592232
1112	13	-0.9233661920921961
1112	14	-0.8319939971823445
1112	15	-0.817131705196708
1112	16	-0.9971674048950234
1112	17	-1.0678288646693233
1112	18	-0.8338581822418689
1112	19	-0.8395227930758793
1112	20	-0.943565975847477
1112	21	-0.8437373764160156
1112	22	-0.8480885046264035
1112	23	-0.9326347326851376
1112	24	-0.8421615426832219
1112	25	-0.8971719183131981
1113	1	-0.8622766949890064
1113	2	-0.9940489776484682
1113	3	-0.8819747203086372
1113	4	-0.7820579904624481
1113	5	-0.8973758923767505
1113	6	-0.9767998093165247
1113	7	-1.207607372834147
1113	8	-1.20450420494506
1113	9	-1.1779870829222148
1113	10	-1.231916488515978
1113	11	-1.2483101454131997
1113	12	-1.1420066170233625
1113	13	-1.1812271347609595
1113	14	-1.2208618175425485
1113	15	-1.2907295077236185
1113	16	-1.278258578549547
1113	17	-1.3359861364629282
1113	18	-1.2965769199049078
1113	19	-1.2756028265946
1113	20	-1.4804068965233554
1113	21	-1.2744549584695832
1113	22	-1.4144456272798251
1113	23	-1.831008560248236
1113	24	-1.5790144127240495
1113	25	-1.6262271168163522
1114	1	-2.5485520637522114
1114	2	-2.8269999342694234
1114	3	-2.446652676496921
1114	4	-5.500368445515107
1114	5	-3.1208374663337466
1114	6	-2.6552553126287144
1114	7	-2.319121282581097
1114	8	-2.5837757994811206
1114	9	-3.317805658735761
1114	10	-2.968807431449516
1114	11	-2.7205050940134754
1114	12	-2.852271579994227
1114	13	-2.9284537549878955
1114	14	-2.791275664126033
1114	15	-3.3587652556114804
1114	16	-3.341296082309988
1114	17	-3.0852912783530115
1114	18	-3.131911248831976
1114	19	-3.0872204706570514
1114	20	-3.42446623422979
1114	21	-3.3181741482991853
1114	22	-3.4499846681778763
1114	23	-3.6315845823398725
1114	24	-3.553316708063015
1114	25	-3.4671318766093826
1115	1	-2.8789279138894273
1115	2	-2.39605672940127
1115	3	-1.9070840092734365
1115	4	-1.9699227946381228
1115	5	-1.9352438617519532
1115	6	-1.9278427127003042
1115	7	-2.069110544005749
1115	8	-1.995910389625493
1115	9	-2.2516059212342725
1115	10	-2.2279501351841517
1115	11	-2.202662354483593
1115	12	-2.6450128996458204
1115	13	-2.669308068315658
1115	14	-2.968729428593278
1115	15	-2.8963735362462515
1115	16	-2.6579504195012547
1115	17	-2.6217785710388455
1115	18	-2.502497694363541
1115	19	-2.5689332734252233
1115	20	-2.5603849790096547
1115	21	-2.4988262184578787
1115	22	-2.6366527268820903
1115	23	-2.66177191082096
1115	24	-2.869248577768303
1115	25	-2.9726974415777647
1116	1	-1.6365192153962127
1116	2	-1.6479702816182566
1116	3	-1.7691295783469378
1116	4	-1.5855748493719588
1116	5	-1.7413451005175062
1116	6	-1.870359643972904
1116	7	-1.9463347013184524
1116	8	-1.9780438130374414
1116	9	-2.28372472532989
1116	10	-2.681705887386741
1116	11	-2.420167158368784
1116	12	-2.376393511331308
1116	13	-2.3278827957263246
1116	14	-3.017457049687181
1116	15	-3.1629986615348997
1116	16	-3.063538210157077
1116	17	-2.780304567395092
1116	18	-2.5753705284619883
1116	19	-2.637626865845327
1116	20	-2.5960411315821545
1116	21	-2.735530476333757
1116	22	-2.7029283729838767
1116	23	-3.0114116886516555
1116	24	-3.0966438752215453
1116	25	-3.01813784848207
1201	1	1.084524840252982
1201	2	1.064285859044766
1201	3	1.0125196629164428
1201	4	1.1350727270815355
1201	5	1.158719416349257
1201	6	1.1203950839857697
1201	7	1.159832581833932
1201	8	1.2057913569038377
1201	9	1.3728410111436986
1201	10	1.4149853775789438
1201	11	1.385707551472521
1201	12	1.3880569615274894
1201	13	1.410002276511925
1201	14	1.745386111039501
1201	15	1.6158454516623948
1201	16	1.6314539692134744
1201	17	1.477146641679468
1201	18	1.6522963047408084
1201	19	1.6665593374122167
1201	20	1.5965138118546562
1201	21	1.517288873346871
1201	22	1.6118895928671293
1201	23	1.5981922629363154
1201	24	1.6522583635662258
1201	25	1.6961270098328924
1202	1	0.9715466972898312
1202	2	1.0264811683165362
1202	3	0.9749670106711363
1202	4	1.0389054427583275
1202	5	0.96661308856077
1202	6	0.8987245339793901
1202	7	0.8710592195972993
1202	8	0.9720283682682904
1202	9	1.0835657353616739
1202	10	1.141754027885824
1202	11	1.1680966599485885
1202	12	1.1248801083956153
1202	13	1.1626183926962597
1202	14	1.5591221001877855
1202	15	1.3385663422993233
1202	16	1.3790120083757174
1202	17	1.361720588472302
1202	18	1.57136166383615
1202	19	1.390660490298977
1202	20	1.3684741520308918
1202	21	1.4075915575026272
1202	22	1.383647653230888
1202	23	1.478385283309244
1202	24	1.5102135850896659
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1203	1	0.9234932868957877
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2211	8	-0.12413618101009405
2211	9	-0.13464609610799982
2211	10	-0.14385956578581016
2211	11	-0.048955130977766714
2211	12	-0.12763173967496755
2211	13	-0.17250225413768305
2211	14	0.13198003065578234
2211	15	-0.08181825609329962
2211	16	-0.16753210508974092
2211	17	-0.2826437227144112
2211	18	-0.09399779089284266
2211	19	-0.10476585424515861
2211	20	-0.050176195465716944
2211	21	-0.12996728825167736
2211	22	-0.18410095915027824
2211	23	-0.0644361196231884
2211	24	-0.027369650426841474
2211	25	0.017075340895004842
2212	1	-0.6008665349467677
2212	2	-0.6210820384101758
2212	3	-0.6574242714331007
2212	4	-0.48954980848147756
2212	5	-0.5605368150620436
2212	6	-0.49002464954421754
2212	7	-0.5680152706010446
2212	8	-0.5806992463078657
2212	9	-0.5560378167135767
2212	10	-0.7918726945193768
2212	11	-0.727512798463664
2212	12	-0.6951217038717488
2212	13	-0.6696291801961891
2212	14	-0.688819021259178
2212	15	-0.7948982906630704
2212	16	-0.7961649393960997
2212	17	-0.9374431929663771
2212	18	-0.7593111798368994
2212	19	-0.8622965220479344
2212	20	-0.8185546106502031
2212	21	-0.8896007783078659
2212	22	-0.8954863982816832
2212	23	-1.0205286440591053
2212	24	-0.8830044530739372
2212	25	-0.8523648395297556
2213	1	-0.6846894633838154
2213	2	-0.500639344594898
2213	3	-0.5500509607854482
2213	4	-0.3232618128256419
2213	5	-0.4289612742795228
2213	6	-0.4640249614920222
2213	7	-0.5249025868169213
2213	8	-0.5251417893048895
2213	9	-0.30923865153112473
2213	10	-0.5342600220093559
2213	11	-0.6136569428238161
2213	12	-0.5930948941256844
2213	13	-0.6807484858209918
2213	14	-0.38876389929920663
2213	15	-0.6510107501454172
2213	16	-0.5723121690703721
2213	17	-0.7010346787417276
2213	18	-0.6847836686430853
2213	19	-0.6690608511952334
2213	20	-0.6473510145168859
2213	21	-0.6118008836942934
2213	22	-0.8199677602077884
2213	23	-0.8322572728236395
2213	24	-0.7468374965210884
2213	25	-0.7649800411028878
2214	1	-1.184252658764617
2214	2	-1.0168441204339693
2214	3	-0.907330208434427
2214	4	-0.8761771458921785
2214	5	-0.8786748813586271
2214	6	-1.109354568937846
2214	7	-1.037706836886052
2214	8	-1.0428414538604187
2214	9	-1.1747998160901219
2214	10	-1.3270508760130468
2214	11	-1.2789406562300414
2214	12	-1.1571334851684156
2214	13	-1.4082149737930365
2214	14	-1.2146447539332001
2214	15	-1.3498948536720903
2214	16	-1.302632815145536
2214	17	-1.3056052944329721
2214	18	-1.3061219542784457
2214	19	-1.5034759826573207
2214	20	-1.5035855681025083
2214	21	-1.450630835993806
2214	22	-1.4000288537453187
2214	23	-1.5274111360745977
2214	24	-1.6193311400247055
2214	25	-1.6099205233069611
2215	1	-1.6448990457155723
2215	2	-1.7566003993904467
2215	3	-1.6204906989405679
2215	4	-1.7899523025995911
2215	5	-1.690646359913309
2215	6	-1.4813859701161576
2215	7	-1.6090624104515783
2215	8	-1.789663192433384
2215	9	-2.0255953258730273
2215	10	-1.7320434305687158
2215	11	-1.9755800251783242
2215	12	-2.0620886815918524
2215	13	-2.015722566979278
2215	14	-2.8889172989376277
2215	15	-2.4367983372842588
2215	16	-2.2366859121676157
2215	17	-2.179697886047549
2215	18	-2.0724677080561875
2215	19	-2.276208286430247
2215	20	-2.476457968611605
2215	21	-2.1759670554435857
2215	22	-2.679837523854154
2215	23	-2.293660838907634
2215	24	-2.293560656599702
2215	25	-2.221019135161356
2216	1	-1.7512223458143659
2216	2	-1.6759926685852804
2216	3	-1.7153711388993322
2216	4	-1.6310578208906676
2216	5	-2.1732701085316215
2216	6	-1.8272042082019269
2216	7	-1.914110054111969
2216	8	-2.375228803291801
2216	9	-2.4174331789241634
2216	10	-2.3133972641184357
2216	11	-2.2833244027006643
2216	12	-2.5093886597016137
2216	13	-2.483157653634983
2216	14	-2.3155282320997657
2216	15	-2.4477171996142957
2216	16	-2.438728947168727
2216	17	-2.557463586628998
2216	18	-2.8601619672747205
2216	19	-2.673211227628798
2216	20	-2.735812800365565
2216	21	-2.9612524318094984
2216	22	-3.1180188677294325
2216	23	-2.8562424509144435
2216	24	-2.98309427275467
2216	25	-2.8808866500823243
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	139532.0
3	8.0
4	1030.0
5	5981.0
6	7948.0
7	7062.0
8	6506.0
9	6014.0
10	5592.0
11	5359.0
12	5254.0
13	4913.0
14	4838.0
15	5069.0
16	5131.0
17	5482.0
18	6199.0
19	7396.0
20	9756.0
21	12367.0
22	15334.0
23	18367.0
24	21685.0
25	25059.0
26	28151.0
27	31539.0
28	35504.0
29	42155.0
30	52676.0
31	66756.0
32	84412.0
33	110381.0
34	147881.0
35	202403.0
36	294607.0
37	504183.0
38	1409204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.986478683981225	17.894058029481148	12.526053578364696	25.593409708172928
2	15.98985727225326	20.565922760200337	38.96657556726771	24.477644400278688
3	18.537805213793483	24.76925412499104	29.140217465298505	27.55272319591697
4	12.45307027281373	16.844273887957883	38.34496478632458	32.35769105290381
5	14.7530655710885	36.06649394598858	34.29715649620266	14.883283986720253
6	31.57376589759545	37.25123210602364	17.396267295062085	13.778734701318818
7	28.028944807200347	31.29480252988342	22.2514033982879	18.42484926462834
8	25.774532499435804	34.10139526278951	20.619168012756138	19.504904225018553
9	26.733778772284122	15.390451031793638	19.459146286534125	38.416623909388115
10	15.205517216444992	27.53884000463986	32.94759216827631	24.30805061063884
11	34.9203804863645	22.73921257280252	23.481142905351458	18.85926403548153
12	23.76741280903	24.536132981689978	30.013350837161667	21.683103372118353
13	28.845526348693674	21.319596450183713	25.222225067752923	24.61265213336969
14	22.593430801193524	20.579992817650265	27.008888760345716	29.817687620810496
15	24.08622618963688	27.85460726873269	23.51099927147565	24.548167270154785
16	24.064853835107176	27.27641975107765	24.740854035180334	23.91787237863484
17	23.632004946422793	26.402164421552378	25.941932564010315	24.023898068014518
18	24.153479079484672	25.91547309750293	26.829462299564895	23.101585523447508
19	25.28814072029305	25.917057894486295	25.94549987074568	22.849301514474977
20	24.623236406499714	25.887853170671736	26.1710284947053	23.317881928123242
21	24.65430040235956	25.76472119561713	25.973644454434304	23.60733394758901
22	23.995827504303413	25.732740321500312	26.413573024851477	23.857859149344794
23	23.66836127141156	25.47559817378015	26.247661782926677	24.608378771881615
24	23.588586167983234	26.10608975759393	26.37740857980989	23.927915494612947
25	23.958459810930616	25.671123398247538	26.247544890947815	24.12287189987403
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12585.0
1	12585.0
2	11655.5
3	10726.0
4	10726.0
5	10726.0
6	7874.0
7	5022.0
8	5022.0
9	5022.0
10	7085.5
11	9149.0
12	9149.0
13	9149.0
14	13413.0
15	17677.0
16	17677.0
17	17677.0
18	29353.0
19	41029.0
20	41029.0
21	41029.0
22	59430.0
23	77831.0
24	77831.0
25	77831.0
26	105745.0
27	133659.0
28	133659.0
29	133659.0
30	161485.0
31	189311.0
32	189311.0
33	189311.0
34	220785.0
35	252259.0
36	252259.0
37	252259.0
38	281971.0
39	311683.0
40	311683.0
41	311683.0
42	339547.5
43	367412.0
44	367412.0
45	367412.0
46	387298.0
47	407184.0
48	407184.0
49	407184.0
50	408464.0
51	409744.0
52	409744.0
53	409744.0
54	381231.0
55	352718.0
56	352718.0
57	352718.0
58	315340.5
59	277963.0
60	277963.0
61	277963.0
62	237020.5
63	196078.0
64	196078.0
65	196078.0
66	160071.5
67	124065.0
68	124065.0
69	124065.0
70	95792.0
71	67519.0
72	67519.0
73	67519.0
74	52544.0
75	37569.0
76	37569.0
77	37569.0
78	29702.5
79	21836.0
80	21836.0
81	21836.0
82	16536.0
83	11236.0
84	11236.0
85	11236.0
86	8205.5
87	5175.0
88	5175.0
89	5175.0
90	3621.5
91	2068.0
92	2068.0
93	2068.0
94	1130.0
95	192.0
96	192.0
97	192.0
98	118.0
99	44.0
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23158635606544387
2	0.6558571089141146
3	0.6537923126137508
4	0.6626200649124078
5	0.6791384353153183
6	0.6951181632050906
7	0.6883851317908607
8	0.6841059162698169
9	0.6766247702540059
10	0.678360396129674
11	0.6829987066594768
12	0.683507424588552
13	0.6789588878109389
14	0.6728841972461004
15	0.6791683598993816
16	0.686709355083319
17	0.6874275451008369
18	0.6812930053678719
19	0.6791683598993816
20	0.6865298075789396
21	0.6826396116507178
22	0.6839862179335638
23	0.6872479975964574
24	0.6921257047987661
25	0.6773429602715237
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3341734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.81591156363847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.38644357855276	59.05620797569101
2	6.99051720642707	9.341555588974483
3	1.7443030051385824	3.49641585994585
4	0.7727748095735222	2.0653461334028806
5	0.44847670811041407	1.498269003372856
6	0.30072684675053674	1.205600303837743
7	0.22219005404412734	1.0392081700932725
8	0.15406920807626157	0.8235419665202646
9	0.11936976550392651	0.7178219725754353
>10	0.7595625959636759	9.513678933889482
>50	0.05880225289068957	2.6943769201749226
>100	0.037667795886443915	4.915959221090308
>500	0.0038785034106349273	1.7510116099117112
>1k	0.0010823730411651292	1.2355827817738216
>5k	1.3529663014564115E-4	0.6454235587458171
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8093	0.24217965882383216	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	7576	0.22670864886313513	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5750	0.17206635836365192	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4708	0.14088494176975186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.984916812648763E-5
2	0.0	0.0	0.0	0.0	5.984916812648763E-5
3	0.0	0.0	0.0	0.0	5.984916812648763E-5
4	0.0	0.0	0.0	0.0	5.984916812648763E-5
5	0.0	0.0	0.0	0.0	5.984916812648763E-5
6	0.0	0.0	0.0	0.0	5.984916812648763E-5
7	0.0	0.0	0.0	5.984916812648763E-5	5.984916812648763E-5
8	0.0	0.0	0.0	5.984916812648763E-5	5.984916812648763E-5
9	0.0	0.0	0.0	8.977375218973144E-5	5.984916812648763E-5
10	0.0	0.0	0.0	1.1969833625297526E-4	5.984916812648763E-5
11	0.0	0.0	0.0	1.1969833625297526E-4	5.984916812648763E-5
12	0.0	0.0	0.0	1.1969833625297526E-4	1.7954750437946287E-4
13	0.0	0.0	0.0	1.4962292031621906E-4	2.0947208844270668E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1505	0.0	15.024936	1
GTATCAA	4550	0.0	13.113554	1
AGAACCG	245	0.0	12.021752	5
AACCGCG	190	0.0	11.999714	7
GATTACG	115	7.08842E-8	11.564593	12
TCGACGC	125	2.2434506E-7	10.639747	14
CGAGCCG	350	0.0	10.585463	15
ACCGTCC	150	2.4499968E-8	10.132787	8
ACTGTCG	95	1.645996E-4	9.99946	8
CGATAAC	190	1.0004442E-10	9.99931	10
GAACCGC	260	0.0	9.865596	6
ACGCTTA	135	6.452701E-7	9.851469	17
TAGAACC	435	0.0	9.828685	4
CGCTTAC	145	1.6198283E-7	9.827205	18
CGAACGA	175	2.577508E-9	9.771196	16
ATCGCTC	185	6.493792E-10	9.756525	16
GATAACG	185	6.493792E-10	9.75623	11
GCCGCCT	390	0.0	9.743212	18
CGCAGAT	205	4.1836756E-11	9.731183	8
ATTACCG	285	0.0	9.666437	15
>>END_MODULE