FastQCFastQC Report
Thu 2 Feb 2017
SRR4050241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341734
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT131510.3935382050157194No Hit
TATCAACGCAGAGTACTTTTTTTTT84970.2542691907853827No Hit
GGTATCAACGCAGAGTACTTTTTTT46950.14049592217692972No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38420.11497025197098273No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36910.11045163977743291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.014.4914561
CGACTCG400.005298807314.24268318
GTATCAA51050.013.2555381
GTATTAG5100.010.46532251
GTATAAG2750.010.3973661
CGCAATA1659.295036E-1010.3648292
AACCGCG1502.4572728E-810.1308587
GTCCTAG3300.010.108551
TCGGACG856.6092703E-410.05681913
ACGAACG2054.1836756E-119.72962315
CGCATCG2151.0913936E-119.71899313
TTCGGAA2550.09.68130118
CGAACGT1201.6963713E-59.5025164
CGGTCCA3100.09.49824910
CTCGACG800.00452517679.49810713
CGACTCA1201.7066224E-59.497688
ATTCCGA2401.8189894E-129.4975396
GAACCGC1901.0586518E-99.4975386
CGACCAT3050.09.3425410
CCGATAA2151.07320375E-109.277369
Produced by FastQC (version 0.11.5)