##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3341734
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.845127110655724	31.0	31.0	34.0	27.0	34.0
2	30.81167711134399	31.0	31.0	34.0	27.0	34.0
3	31.028044721692392	31.0	31.0	34.0	27.0	34.0
4	34.646216006420616	37.0	35.0	37.0	31.0	37.0
5	34.57562510959879	37.0	35.0	37.0	32.0	37.0
6	34.53087409111557	37.0	35.0	37.0	31.0	37.0
7	34.47158541044859	37.0	35.0	37.0	31.0	37.0
8	34.44535770950052	37.0	35.0	37.0	31.0	37.0
9	36.031666494101565	39.0	37.0	39.0	31.0	39.0
10	35.91853331234623	39.0	37.0	39.0	31.0	39.0
11	35.916955987520254	39.0	37.0	39.0	31.0	39.0
12	35.84218612253399	39.0	37.0	39.0	30.0	39.0
13	35.84577318242565	39.0	37.0	39.0	30.0	39.0
14	37.01668295561526	40.0	37.0	41.0	31.0	41.0
15	36.99203736742661	40.0	37.0	41.0	31.0	41.0
16	36.974726294791864	40.0	37.0	41.0	31.0	41.0
17	36.95312792699838	40.0	37.0	41.0	31.0	41.0
18	36.91973957232982	40.0	37.0	41.0	31.0	41.0
19	36.97881010277898	40.0	37.0	41.0	31.0	41.0
20	36.92928551464599	40.0	37.0	41.0	31.0	41.0
21	36.881612360529	40.0	37.0	41.0	31.0	41.0
22	36.825351449277534	40.0	37.0	41.0	31.0	41.0
23	36.73578746842208	40.0	37.0	41.0	30.0	41.0
24	36.68112123825535	40.0	37.0	41.0	30.0	41.0
25	36.46639020340937	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7943360096465852
1101	2	0.9248186254424873
1101	3	0.8601287754855562
1101	4	0.7856659904271552
1101	5	0.8599780176502279
1101	6	0.8671063510569468
1101	7	0.8883958833844119
1101	8	0.8796518290579627
1101	9	0.9632528659514179
1101	10	0.8944178573427024
1101	11	0.9618605726976739
1101	12	1.003121138731636
1101	13	1.0321424976730498
1101	14	1.1447973756953616
1101	15	1.2675039558506427
1101	16	1.1928321553856875
1101	17	1.2348956504650488
1101	18	1.2829278828863622
1101	19	1.2416586101531522
1101	20	1.2143733873074893
1101	21	1.187649024590172
1101	22	1.2460386668294277
1101	23	1.3089775990842227
1101	24	1.358298948041373
1101	25	1.3406634743445878
1102	1	0.6534633423381138
1102	2	0.814361128622231
1102	3	0.6682715583423366
1102	4	0.7177268986513923
1102	5	0.7610367029807676
1102	6	0.8505011126170459
1102	7	0.7463927376033439
1102	8	0.7900278119681445
1102	9	0.9108131288571997
1102	10	0.9557488764305475
1102	11	0.9674857351984656
1102	12	1.0705791696085427
1102	13	1.0378915121277146
1102	14	1.131038682635051
1102	15	1.0587970170961682
1102	16	1.0935757941385233
1102	17	0.9772001400544568
1102	18	1.0467164409568213
1102	19	1.0868676769360022
1102	20	1.038760393334364
1102	21	1.0787875490478598
1102	22	1.0890959889100884
1102	23	1.1034867120114384
1102	24	1.130211960050623
1102	25	1.1864411474972343
1103	1	0.6159956334452481
1103	2	0.7312161049683077
1103	3	0.7219187235054498
1103	4	0.5885482685768153
1103	5	0.6923904804102818
1103	6	0.8132642484180508
1103	7	0.7710246182858711
1103	8	0.7483724578053241
1103	9	0.8307861793403433
1103	10	0.8858916242175425
1103	11	0.8908522583738545
1103	12	0.9053904380597615
1103	13	0.929287019817572
1103	14	0.9416308897748635
1103	15	0.9933291556063253
1103	16	0.9949353552161568
1103	17	1.031233670733549
1103	18	1.0204236758634408
1103	19	0.9713869922879539
1103	20	1.050173282599367
1103	21	1.0387359946610957
1103	22	0.9753634863948832
1103	23	1.1173227744080094
1103	24	1.1421654825763596
1103	25	1.1429863742610706
1104	1	0.45370381769317447
1104	2	0.6554800751723064
1104	3	0.5733526838018577
1104	4	0.5109197444751814
1104	5	0.5946461495388675
1104	6	0.5752115950583985
1104	7	0.5975383552232145
1104	8	0.5598180897554812
1104	9	0.7531971968489373
1104	10	0.6282928561761238
1104	11	0.7334955551056765
1104	12	0.7679643039844137
1104	13	0.7452227769051589
1104	14	0.8708170581994921
1104	15	0.9332246210800861
1104	16	0.9298661074719945
1104	17	0.7937195998448914
1104	18	0.760217735524229
1104	19	0.814332686923315
1104	20	0.8563262855538909
1104	21	0.8244280560504436
1104	22	0.8761198139955724
1104	23	0.9000156201946155
1104	24	0.9425014753159644
1104	25	0.9360093880551474
1105	1	0.43118489467003585
1105	2	0.5610346607747232
1105	3	0.49431636918528454
1105	4	0.46142230650988125
1105	5	0.5232785227615864
1105	6	0.5541332883820971
1105	7	0.5470103980517749
1105	8	0.5032351512550406
1105	9	0.5477513985214983
1105	10	0.6161816066024173
1105	11	0.6401914633571693
1105	12	0.659896071713284
1105	13	0.6350545555728146
1105	14	0.7334749252224881
1105	15	0.7383843809416177
1105	16	0.7936089731330043
1105	17	0.7317620723531562
1105	18	0.737073071184021
1105	19	0.7127572876040986
1105	20	0.7827552016920265
1105	21	0.7278116577414551
1105	22	0.7661846102017478
1105	23	0.7831923287360922
1105	24	0.8155374596241742
1105	25	0.9534257331716205
1106	1	0.40001247110556903
1106	2	0.506707881172165
1106	3	0.39719510469210917
1106	4	0.38895349670575285
1106	5	0.4332392352633079
1106	6	0.47737361796297506
1106	7	0.43847363418017693
1106	8	0.41766115628936973
1106	9	0.573366725623714
1106	10	0.6262695309851907
1106	11	0.5134157855184398
1106	12	0.5803160346563772
1106	13	0.49317048187335644
1106	14	0.5821375466593963
1106	15	0.6487685022395979
1106	16	0.6172366238502036
1106	17	0.5845227742244035
1106	18	0.597670702908708
1106	19	0.6554639723535374
1106	20	0.6939161209778533
1106	21	0.7303432243646952
1106	22	0.6966678960448931
1106	23	0.785447354946541
1106	24	0.6951635565808374
1106	25	0.7373759233147581
1107	1	0.25019256187039574
1107	2	0.44900574488190514
1107	3	0.34912143474267765
1107	4	0.3489863040515502
1107	5	0.3838720268991693
1107	6	0.4086694638239834
1107	7	0.42373698641948465
1107	8	0.4470776160149441
1107	9	0.4701162955582845
1107	10	0.4795616287687068
1107	11	0.5153993429266635
1107	12	0.5045952899231381
1107	13	0.48204075157878634
1107	14	0.4986034027429369
1107	15	0.5274690880240342
1107	16	0.46808517793094495
1107	17	0.4592615846721628
1107	18	0.5191417873317405
1107	19	0.47130816269499576
1107	20	0.4790423237352286
1107	21	0.5483748215866129
1107	22	0.5114365174014992
1107	23	0.5144337136436192
1107	24	0.5398648269261344
1107	25	0.5252160056582369
1108	1	0.18530509999023792
1108	2	0.315903290613182
1108	3	0.26361114231839977
1108	4	0.18897636935203366
1108	5	0.24205997534207313
1108	6	0.283150990426833
1108	7	0.2631852396452743
1108	8	0.2587689804844544
1108	9	0.2861097273352726
1108	10	0.27305896613504643
1108	11	0.2996792040753178
1108	12	0.37471336107369524
1108	13	0.31210953637809524
1108	14	0.4476422965285707
1108	15	0.39812484528729186
1108	16	0.31552545810150434
1108	17	0.4156838681677044
1108	18	0.3350354633733872
1108	19	0.36253600502587346
1108	20	0.43042052229085925
1108	21	0.43559913116857274
1108	22	0.40288330564085584
1108	23	0.5027287836280294
1108	24	0.4205875844137381
1108	25	0.48772936587021576
1109	1	0.1633127971368431
1109	2	0.2760315131327786
1109	3	0.3145247326460847
1109	4	0.2955917547117295
1109	5	0.3102932894119803
1109	6	0.28893902084892886
1109	7	0.38087889198023106
1109	8	0.32898698179538144
1109	9	0.4047160830224712
1109	10	0.406566621708194
1109	11	0.3258951977088955
1109	12	0.3935655458479701
1109	13	0.398076546067351
1109	14	0.4687046065719045
1109	15	0.5227584709874762
1109	16	0.5028353186018322
1109	17	0.4277056669861494
1109	18	0.49058316470621577
1109	19	0.4599256456853027
1109	20	0.480273755046305
1109	21	0.41953043658976696
1109	22	0.46794822342948805
1109	23	0.5235869205513097
1109	24	0.5681901610605067
1109	25	0.5403370980382931
1110	1	-0.16314924717140045
1110	2	0.04951041244964216
1110	3	-0.04534406643455213
1110	4	-0.07372606435230722
1110	5	-0.08987749523974742
1110	6	-0.13250003935664978
1110	7	-0.11580916267085684
1110	8	-0.058853789448953364
1110	9	-0.021136244117919034
1110	10	-0.08957077214994058
1110	11	-0.011093246395041945
1110	12	0.06039182433868007
1110	13	0.0055272260137257945
1110	14	-0.041997366230241084
1110	15	-0.14085960983994994
1110	16	0.022794777198527072
1110	17	-0.12694542156648936
1110	18	-0.12005177112983034
1110	19	-0.17310282906410634
1110	20	-0.14221235872500415
1110	21	-0.16650831716111014
1110	22	-0.16807297302074886
1110	23	-0.1408052776227251
1110	24	-0.10050357577151203
1110	25	-0.08733640001444343
1111	1	-0.3885953834822793
1111	2	-0.27988711443460446
1111	3	-0.28671483035658696
1111	4	-0.2956467978885584
1111	5	-0.20324287057588464
1111	6	-0.4098802372163419
1111	7	-0.3508244729318619
1111	8	-0.247435125536569
1111	9	-0.38837143789574924
1111	10	-0.3069467446905847
1111	11	-0.3745613204581062
1111	12	-0.2984699999036948
1111	13	-0.3816082424098397
1111	14	-0.28283605293848524
1111	15	-0.2779066595500481
1111	16	-0.22780270117279855
1111	17	-0.4162230224198993
1111	18	-0.3598838146975609
1111	19	-0.38109383330768054
1111	20	-0.3647254846003847
1111	21	-0.36627507694128525
1111	22	-0.43521903082415747
1111	23	-0.4568099124325755
1111	24	-0.3530559814647418
1111	25	-0.39477074455530925
1112	1	-0.4491480993602579
1112	2	-0.33415937703281884
1112	3	-0.4243921206061074
1112	4	-0.3705105580008663
1112	5	-0.3733723236772519
1112	6	-1.3267907369565037
1112	7	-0.640944463314014
1112	8	-0.5050311294767837
1112	9	-0.5847419421345776
1112	10	-0.5331237285651795
1112	11	-0.5837407786010829
1112	12	-0.5646752168093911
1112	13	-0.5033087395904161
1112	14	-0.5883905139900918
1112	15	-0.5893506549893246
1112	16	-0.648663808621194
1112	17	-0.7200653518000948
1112	18	-0.7115671703949715
1112	19	-0.7150319533290883
1112	20	-0.6614459543235611
1112	21	-0.7690816263839935
1112	22	-0.8009180698152107
1112	23	-0.7145846221520316
1112	24	-0.7426006074934222
1112	25	-0.7106848665002872
1113	1	-0.5854718696722863
1113	2	-0.6403779778075602
1113	3	-0.5405262136568716
1113	4	-0.4842484857389735
1113	5	-0.5760551527423416
1113	6	-1.01660733689328
1113	7	-0.6425093763483503
1113	8	-0.6231705377260823
1113	9	-0.736034908798878
1113	10	-0.6365828730814087
1113	11	-0.6999428771515781
1113	12	-0.7046822049190169
1113	13	-0.6719476766717136
1113	14	-0.9266132350268705
1113	15	-0.7668615568941135
1113	16	-0.8072767444894069
1113	17	-0.7967539256358265
1113	18	-0.7712191262337527
1113	19	-0.7824129701746685
1113	20	-0.8313970526819645
1113	21	-0.8783121931620386
1113	22	-0.859216419744044
1113	23	-0.8834966400367534
1113	24	-0.9001374349824118
1113	25	-0.8917674487126703
1114	1	-0.842546657858918
1114	2	-0.8167589146644652
1114	3	-0.9386732586971007
1114	4	-0.7496884187161825
1114	5	-0.9503265854744427
1114	6	-1.5691078672507004
1114	7	-1.1257935891960642
1114	8	-1.3247809066031522
1114	9	-1.4085647915928803
1114	10	-1.602347372813533
1114	11	-1.2678919700306466
1114	12	-1.3562895774264874
1114	13	-1.4492012162831216
1114	14	-1.7575854490923106
1114	15	-1.5822365285784201
1114	16	-1.5445564364146733
1114	17	-1.6030643998652465
1114	18	-1.6830546063660208
1114	19	-1.5397978226873619
1114	20	-1.5177462519920795
1114	21	-1.5984734457883576
1114	22	-1.5229426690204235
1114	23	-1.5251290382231062
1114	24	-1.5891085176186834
1114	25	-1.649034543900143
1115	1	-1.3579201216460568
1115	2	-2.0201057706482395
1115	3	-1.6024669587328972
1115	4	-1.2848321963083507
1115	5	-1.4448916731728119
1115	6	-1.9772906616666361
1115	7	-1.5598068334389978
1115	8	-1.5905845099917642
1115	9	-1.6730403911633402
1115	10	-1.6909245115797873
1115	11	-1.7443506265155548
1115	12	-1.6927154215978248
1115	13	-1.747742559798219
1115	14	-1.9844438192152083
1115	15	-1.8699808311676094
1115	16	-1.961828241688508
1115	17	-1.915269387336906
1115	18	-2.143478780092309
1115	19	-2.0731542240714447
1115	20	-2.05066595131197
1115	21	-2.142502797894309
1115	22	-2.224123912308812
1115	23	-2.174258617032322
1115	24	-2.200958535516314
1115	25	-2.15682776666592
1116	1	-0.9715111850351903
1116	2	-1.0665666778211182
1116	3	-1.047413797582621
1116	4	-0.8664280829643545
1116	5	-0.9556617899612121
1116	6	-1.1185354632260314
1116	7	-1.1559825808792326
1116	8	-1.140393738988962
1116	9	-1.25872891112369
1116	10	-1.21775679922591
1116	11	-1.3180261662950485
1116	12	-1.2123811431773746
1116	13	-1.3451726888801332
1116	14	-1.5975566260685667
1116	15	-1.497708496562474
1116	16	-1.4985381479365998
1116	17	-1.4587625732957221
1116	18	-1.5130506943921063
1116	19	-1.5140936812739554
1116	20	-1.4666832397610463
1116	21	-1.5630992549251488
1116	22	-1.47564454271145
1116	23	-1.571542608327178
1116	24	-1.6595064331589455
1116	25	-1.7599684554350716
1201	1	0.6925209889674662
1201	2	0.8907540591771088
1201	3	0.8833883928121118
1201	4	0.7266635493253233
1201	5	0.743016969908247
1201	6	0.8326466424085979
1201	7	0.8694564894450139
1201	8	0.8611844107426734
1201	9	1.0191270965213874
1201	10	1.0187911176185978
1201	11	1.0021563148189756
1201	12	1.091859141769845
1201	13	1.0715700241695387
1201	14	1.1907688991009593
1201	15	1.1729233336331077
1201	16	1.1566513821359479
1201	17	1.2060337922421311
1201	18	1.1813510452547575
1201	19	1.2642080688080881
1201	20	1.273942199158249
1201	21	1.255584976400364
1201	22	1.245267041564901
1201	23	1.2377498917003251
1201	24	1.3108171026386373
1201	25	1.3412891164509588
1202	1	0.6988414218246319
1202	2	0.8379492927291636
1202	3	0.8044343633333426
1202	4	0.7682120391496738
1202	5	0.7537075120830465
1202	6	0.8474952786965133
1202	7	0.8334441925631566
1202	8	0.8193590031001747
1202	9	0.9150308762332671
1202	10	0.9738309137378565
1202	11	0.9491503502687806
1202	12	0.9697632633021485
1202	13	0.943154794554907
1202	14	1.1509619627423007
1202	15	1.109882343305756
1202	16	1.070686788675097
1202	17	1.164340353247347
1202	18	1.2115459045350718
1202	19	1.1245928115016781
1202	20	1.139032381339888
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2211	4	-0.06125930422811621
2211	5	-0.10207812419508855
2211	6	-0.09076372376300412
2211	7	-0.07205269669122316
2211	8	-0.06300017140433312
2211	9	-0.12057571777491205
2211	10	-0.06484855235874676
2211	11	-0.1093780595211058
2211	12	-0.14969227947555197
2211	13	-0.11094792433646461
2211	14	-0.22961735399378114
2211	15	-0.31034001359554253
2211	16	-0.23109352912786107
2211	17	-0.3038436183720137
2211	18	-0.28220730232207103
2211	19	-0.16194418253270726
2211	20	-0.16273429405950424
2211	21	-0.10117374506475585
2211	22	-0.2033847469447707
2211	23	-0.21714431388048183
2211	24	-0.24536266946162044
2211	25	-0.42181070277597854
2212	1	-0.473625280884459
2212	2	-0.48091388178726646
2212	3	-0.4647126277057687
2212	4	-0.5406819478143348
2212	5	-0.4564394446931246
2212	6	-0.4691397513389717
2212	7	-0.5049800745241555
2212	8	-0.5351939362187608
2212	9	-0.6494067405992467
2212	10	-0.6158061168940634
2212	11	-0.6459583307494512
2212	12	-0.7145240352401743
2212	13	-0.6965835849049995
2212	14	-0.8622849546275972
2212	15	-0.8451004514711968
2212	16	-0.825727575565196
2212	17	-0.7782491707702306
2212	18	-0.7366496679911734
2212	19	-0.7763228752177085
2212	20	-0.7885838104026845
2212	21	-0.7697223612066608
2212	22	-0.7932355844805485
2212	23	-0.8694458301611405
2212	24	-0.9890489309619142
2212	25	-0.8256410315280718
2213	1	-0.4392992512004703
2213	2	-0.7421182090288383
2213	3	-0.5484757692524873
2213	4	-0.5992832563428223
2213	5	-0.5651317628448567
2213	6	-0.5122510685656039
2213	7	-0.6357546573250232
2213	8	-0.6408213454363292
2213	9	-0.7255029976790297
2213	10	-0.6415875905610946
2213	11	-0.6326360102130764
2213	12	-0.7049993469193296
2213	13	-0.769602230277556
2213	14	-0.7482079961969674
2213	15	-0.8882905010443096
2213	16	-0.9495701091329707
2213	17	-0.9469148211736425
2213	18	-0.9763550953425835
2213	19	-0.7966390195974853
2213	20	-0.8718515748581126
2213	21	-0.9014490308246792
2213	22	-0.8588796644572056
2213	23	-0.9738778121533898
2213	24	-0.9152501986676072
2213	25	-0.8839555493317377
2214	1	-0.7892031837388238
2214	2	-0.8236715675599626
2214	3	-0.8658954494055919
2214	4	-0.710710926485973
2214	5	-0.9063417936690783
2214	6	-0.8343960735853599
2214	7	-0.8224644455302723
2214	8	-0.7720191593625074
2214	9	-0.8424679595189417
2214	10	-0.8451101948382416
2214	11	-0.9173907026810966
2214	12	-1.0274634207647608
2214	13	-0.8794263835463951
2214	14	-1.0673497131189862
2214	15	-1.141242174180313
2214	16	-1.1534662471034025
2214	17	-0.9852199987438865
2214	18	-1.0405774977740236
2214	19	-1.0333009022422814
2214	20	-1.0315427475988983
2214	21	-1.1469683002596085
2214	22	-1.1339674159236992
2214	23	-1.2354765218848982
2214	24	-1.19804683994046
2214	25	-1.2168776015414124
2215	1	-1.483996639048879
2215	2	-3.425061732930665
2215	3	-1.8847540200747765
2215	4	-1.5294279641431814
2215	5	-1.5250169997933583
2215	6	-1.4719517835073148
2215	7	-1.48171869223129
2215	8	-1.486497642379966
2215	9	-1.7050667838972657
2215	10	-1.8497835071730222
2215	11	-1.8239177832874347
2215	12	-1.7897341010044343
2215	13	-1.8842279433419193
2215	14	-2.0587115015696256
2215	15	-2.0213823546992415
2215	16	-2.0056948609192062
2215	17	-2.128517355861753
2215	18	-2.0393578602331814
2215	19	-1.8838582296202588
2215	20	-1.9698951567196659
2215	21	-2.001318832048206
2215	22	-1.9921461912681693
2215	23	-2.2586354519372165
2215	24	-2.178422794110098
2215	25	-2.2405326451959624
2216	1	-1.1523972032623604
2216	2	-1.8330265749961896
2216	3	-1.2937601918589365
2216	4	-1.0241601196774397
2216	5	-1.1861274734575247
2216	6	-1.174434811470391
2216	7	-1.3043009748857415
2216	8	-1.3147789228972968
2216	9	-1.4524689444781913
2216	10	-1.4353134699503443
2216	11	-1.5354452221226467
2216	12	-1.575809792430121
2216	13	-1.719605510572535
2216	14	-1.7702550544967792
2216	15	-1.8156741173174495
2216	16	-1.7262865344374987
2216	17	-1.7525224664633967
2216	18	-2.0859580172412393
2216	19	-1.9033681635666824
2216	20	-1.907656447372645
2216	21	-1.8324376708389138
2216	22	-1.8666982753028165
2216	23	-1.8706761390919482
2216	24	-1.9071530694544236
2216	25	-2.0280646331900414
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45316.0
3	0.0
4	837.0
5	6646.0
6	8124.0
7	6722.0
8	5384.0
9	4703.0
10	4269.0
11	3912.0
12	3608.0
13	3342.0
14	3448.0
15	3604.0
16	3692.0
17	4001.0
18	4308.0
19	5221.0
20	7211.0
21	9695.0
22	12126.0
23	14802.0
24	17749.0
25	20502.0
26	23351.0
27	26454.0
28	31285.0
29	38169.0
30	48545.0
31	63830.0
32	82549.0
33	108760.0
34	147509.0
35	203389.0
36	294112.0
37	506473.0
38	1568086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25810442068044	18.542135060867228	13.092818916400043	26.106941602052284
2	15.777318355969566	21.22596589810612	38.09474357738887	24.901972168535448
3	18.24849015511109	25.055944009906234	29.360146558642906	27.335419276339767
4	12.878613318714176	17.45228674694036	37.51226159831991	32.15683833602555
5	14.521950842710925	36.44599757967985	34.19369866475213	14.838352912857097
6	31.466277634384472	37.75573261511989	17.120652377211687	13.657337373283957
7	27.820629799530344	31.486086168823764	22.198747128249526	18.494536903396362
8	25.73264753797151	34.42434964551146	20.37249724393096	19.47050557258606
9	26.599214346466866	15.410415086750492	19.397519978021332	38.592850588761316
10	15.102557188131602	27.837618059810087	32.83443208730732	24.22539266475099
11	34.93172330206042	22.7738325028804	23.42628420942377	18.86815998563541
12	23.7127958465662	24.748400983052754	29.871749443651037	21.66705372673001
13	28.80841548761468	21.326701730200742	25.309135444348435	24.55574733783614
14	22.72957019414207	20.64983353907156	26.762278831406878	29.858317435379494
15	23.969571115067303	27.89401251587831	23.68151675109158	24.45489961796281
16	23.97582814664601	27.326273034084146	24.83654340821097	23.861355411058874
17	23.575265752181696	26.362382999760893	26.092568335740896	23.969782912316518
18	24.203614661174893	25.895765179889185	26.864785960779525	23.0358341981564
19	25.140636306342056	25.739511455297738	26.39826813398859	22.72158410437162
20	24.50350864960158	25.77236227327097	26.510661512647086	23.213467564480357
21	24.615998774260426	25.516300899913098	26.358039247606573	23.5096610782199
22	23.883798052148975	25.58650688534755	26.732528681217595	23.797166381285884
23	23.548861560284113	25.283850570155032	26.652132863104438	24.51515500645642
24	23.472475592647392	25.960194109676276	26.73673456196044	23.830595735715892
25	23.817898616104895	25.501615217609235	26.69458696364526	23.985899202640606
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	573.0
1	573.0
2	1052.5
3	1532.0
4	1532.0
5	1532.0
6	3860.0
7	6188.0
8	6188.0
9	6188.0
10	8293.5
11	10399.0
12	10399.0
13	10399.0
14	14320.0
15	18241.0
16	18241.0
17	18241.0
18	30490.5
19	42740.0
20	42740.0
21	42740.0
22	62385.5
23	82031.0
24	82031.0
25	82031.0
26	110268.5
27	138506.0
28	138506.0
29	138506.0
30	166437.5
31	194369.0
32	194369.0
33	194369.0
34	224790.0
35	255211.0
36	255211.0
37	255211.0
38	283172.5
39	311134.0
40	311134.0
41	311134.0
42	339599.5
43	368065.0
44	368065.0
45	368065.0
46	387105.5
47	406146.0
48	406146.0
49	406146.0
50	408993.5
51	411841.0
52	411841.0
53	411841.0
54	382618.0
55	353395.0
56	353395.0
57	353395.0
58	315545.0
59	277695.0
60	277695.0
61	277695.0
62	236954.5
63	196214.0
64	196214.0
65	196214.0
66	159945.5
67	123677.0
68	123677.0
69	123677.0
70	95126.0
71	66575.0
72	66575.0
73	66575.0
74	51855.0
75	37135.0
76	37135.0
77	37135.0
78	29438.5
79	21742.0
80	21742.0
81	21742.0
82	16420.5
83	11099.0
84	11099.0
85	11099.0
86	8052.5
87	5006.0
88	5006.0
89	5006.0
90	3497.0
91	1988.0
92	1988.0
93	1988.0
94	1094.5
95	201.0
96	201.0
97	201.0
98	116.0
99	31.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3353348890127102
2	0.010054660245249921
3	0.0
4	0.0
5	0.0502733012262496
6	0.0032617796628935753
7	0.003920120512284939
8	5.386425131383886E-4
9	0.00933647022773207
10	0.015710406633203
11	0.0055061234676368614
12	0.009156922723352607
13	0.01678769165947978
14	0.0032617796628935753
15	0.020887359676144183
16	0.009785338988680727
17	0.00541634971544713
18	2.9924584063243813E-4
19	0.004069743432601159
20	0.004039818848537915
21	0.0023341175569330176
22	0.0
23	0.0029625338222611375
24	0.00353110091946277
25	0.0023640421409962613
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3341734.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.0893119548064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.04365980873483	58.187448987423274
2	7.258786838081462	9.594564555108171
3	1.768792010428406	3.5069474088111634
4	0.8003824646410035	2.115869055552643
5	0.4642031777075839	1.5339434310964473
6	0.2807298280841295	1.1131944709962724
7	0.21238798624316746	0.9825603114794478
8	0.1504253219041077	0.7953204820178198
9	0.12078966953209913	0.7184615535562388
>10	0.7920321728125451	9.782869530977516
>50	0.06340184034439889	2.8660710377986995
>100	0.03932433137477477	5.180898208852967
>500	0.0037226170481625053	1.6306070158270987
>1k	0.0012711375256344354	1.3417374256638641
>5k	4.5397768772658406E-5	0.25493611149073914
>10k+	4.5397768772658406E-5	0.394570413347618
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13151	0.3935382050157194	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8497	0.2542691907853827	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4695	0.14049592217692972	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3842	0.11497025197098273	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3691	0.11045163977743291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.984916812648763E-5
2	0.0	0.0	0.0	0.0	5.984916812648763E-5
3	0.0	0.0	0.0	0.0	5.984916812648763E-5
4	0.0	0.0	0.0	0.0	5.984916812648763E-5
5	0.0	0.0	0.0	0.0	5.984916812648763E-5
6	0.0	0.0	0.0	0.0	5.984916812648763E-5
7	2.9924584063243814E-5	0.0	0.0	2.9924584063243814E-5	5.984916812648763E-5
8	2.9924584063243814E-5	0.0	0.0	2.9924584063243814E-5	5.984916812648763E-5
9	2.9924584063243814E-5	0.0	0.0	5.984916812648763E-5	5.984916812648763E-5
10	2.9924584063243814E-5	0.0	0.0	5.984916812648763E-5	5.984916812648763E-5
11	2.9924584063243814E-5	0.0	0.0	5.984916812648763E-5	5.984916812648763E-5
12	2.9924584063243814E-5	0.0	0.0	5.984916812648763E-5	8.977375218973144E-5
13	2.9924584063243814E-5	0.0	0.0	8.977375218973144E-5	1.4962292031621906E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1710	0.0	14.491456	1
CGACTCG	40	0.0052988073	14.242683	18
GTATCAA	5105	0.0	13.255538	1
GTATTAG	510	0.0	10.4653225	1
GTATAAG	275	0.0	10.397366	1
CGCAATA	165	9.295036E-10	10.364829	2
AACCGCG	150	2.4572728E-8	10.130858	7
GTCCTAG	330	0.0	10.10855	1
TCGGACG	85	6.6092703E-4	10.056819	13
ACGAACG	205	4.1836756E-11	9.729623	15
CGCATCG	215	1.0913936E-11	9.718993	13
TTCGGAA	255	0.0	9.681301	18
CGAACGT	120	1.6963713E-5	9.502516	4
CGGTCCA	310	0.0	9.498249	10
CTCGACG	80	0.0045251767	9.498107	13
CGACTCA	120	1.7066224E-5	9.49768	8
ATTCCGA	240	1.8189894E-12	9.497539	6
GAACCGC	190	1.0586518E-9	9.497538	6
CGACCAT	305	0.0	9.34254	10
CCGATAA	215	1.07320375E-10	9.27736	9
>>END_MODULE