FastQCFastQC Report
Thu 2 Feb 2017
SRR4050240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303892
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN112790.3413852510917427No Hit
GTATCAACGCAGAGTACTTTTTTTT76970.23296766359190918No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN50520.15291056729457259No Hit
TATCAACGCAGAGTACTTTTTTTTT43110.13048247339804087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14000.014.3947521
GTATCAA45600.013.5084811
CGTCGTA2150.011.48787410
CTAGACA2700.010.9072674
CCGGTAG1053.4725072E-610.8563419
CGAGCCG3250.010.81508115
ACCGTCC1553.6725396E-910.4185868
CCTATTC5850.010.3930293
AGGCCCG3800.09.99956610
GCGTTAT1356.3940206E-79.8580411
CGAACGA1451.6195372E-79.82731116
TACCGTC3050.09.6547317
CGGTTCT3450.09.63726312
TATCAAC64250.09.5526482
GTTCTAA2800.09.5059681
TAGATCG800.00451818539.4998785
AAGGCCG1304.2427637E-69.4998785
ACGCTTA800.0045187549.49973317
ACCGTCG3000.09.4992988
ATACCGT2800.09.4988636
Produced by FastQC (version 0.11.5)