##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050240_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3303892 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.769908035734826 31.0 30.0 34.0 26.0 34.0 2 29.814139203097437 31.0 30.0 34.0 25.0 34.0 3 29.9620959159682 31.0 31.0 34.0 26.0 34.0 4 33.39357218698432 37.0 35.0 37.0 28.0 37.0 5 33.435371071451485 37.0 35.0 37.0 28.0 37.0 6 33.47836279151982 37.0 35.0 37.0 28.0 37.0 7 33.37943340762955 37.0 35.0 37.0 28.0 37.0 8 33.353125344290916 37.0 35.0 37.0 27.0 37.0 9 34.82300147825655 39.0 35.0 39.0 27.0 39.0 10 34.70992151075156 39.0 35.0 39.0 27.0 39.0 11 34.73788065711591 39.0 35.0 39.0 27.0 39.0 12 34.660069094268216 39.0 35.0 39.0 27.0 39.0 13 34.65433555334133 39.0 35.0 39.0 27.0 39.0 14 35.70838816765197 40.0 36.0 41.0 27.0 41.0 15 35.69996476882416 40.0 36.0 41.0 27.0 41.0 16 35.693216666888624 40.0 36.0 41.0 27.0 41.0 17 35.660903261971036 40.0 36.0 41.0 27.0 41.0 18 35.59717660262503 40.0 36.0 41.0 27.0 41.0 19 35.64295776012049 40.0 36.0 41.0 27.0 41.0 20 35.60810220188795 40.0 36.0 41.0 26.0 41.0 21 35.530290336366924 40.0 36.0 41.0 26.0 41.0 22 35.43959094304535 40.0 36.0 41.0 25.0 41.0 23 35.36767121927714 40.0 36.0 41.0 25.0 41.0 24 35.31634750772725 40.0 36.0 41.0 25.0 41.0 25 34.97400762494658 39.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.450436965645931 1101 2 1.5491820444189166 1101 3 1.5144892185307768 1101 4 1.6325519702523934 1101 5 1.5913917698654743 1101 6 1.6100325107254605 1101 7 1.6328835578858119 1101 8 1.621219574120083 1101 9 1.8483232156119982 1101 10 1.8556152545022115 1101 11 1.8881055020406734 1101 12 1.9204507949513 1101 13 1.864926423926633 1101 14 2.1860184511651184 1101 15 2.109049701979842 1101 16 2.138676278975204 1101 17 2.1291389410807113 1101 18 2.190735101114271 1101 19 2.181480616532525 1101 20 2.14454049855145 1101 21 2.235923706856525 1101 22 2.229815065381338 1101 23 2.199536033850272 1101 24 2.213245699943471 1101 25 2.264981858524436 1102 1 1.313330143517799 1102 2 1.3526716071302403 1102 3 1.3127927488584312 1102 4 1.436692570002954 1102 5 1.3925309200564797 1102 6 1.4049900232026715 1102 7 1.2998314678149399 1102 8 1.3730355477896978 1102 9 1.6216082031170203 1102 10 1.6178370024109654 1102 11 1.6228711203445059 1102 12 1.558774764194041 1102 13 1.519381917180354 1102 14 1.7568402744994742 1102 15 1.8207106392557648 1102 16 1.7854029481532692 1102 17 1.7961979616099413 1102 18 1.8132437882280712 1102 19 1.7589970364423735 1102 20 1.7796180627395088 1102 21 1.8757759956094588 1102 22 1.9783137657113414 1102 23 1.836850548134933 1102 24 1.8993345796434085 1102 25 1.8252805254899087 1103 1 1.1416231184237375 1103 2 1.223562577811098 1103 3 1.183108747900146 1103 4 1.301388077578686 1103 5 1.247930359107329 1103 6 1.2265753920474225 1103 7 1.1526062733148237 1103 8 1.111842441998931 1103 9 1.345088515764047 1103 10 1.3929420781807238 1103 11 1.3292283871283246 1103 12 1.3259229440079494 1103 13 1.374911027101561 1103 14 1.542084304226158 1103 15 1.6336795100712251 1103 16 1.6611729030112556 1103 17 1.6752744493379055 1103 18 1.5986689706876689 1103 19 1.592050572376742 1103 20 1.5553418622991657 1103 21 1.6290210500184514 1103 22 1.7227515567678182 1103 23 1.684388027946632 1103 24 1.7730712734524303 1103 25 1.7464951406902358 1104 1 0.9174015231670865 1104 2 1.0821451613991933 1104 3 1.057390177365928 1104 4 1.1386586001229233 1104 5 1.0700474408013676 1104 6 1.0785343581594447 1104 7 0.9954860024279739 1104 8 1.0606602005563985 1104 9 1.167275630010245 1104 10 1.2139610400247705 1104 11 1.210511185412173 1104 12 1.2465977559870538 1104 13 1.259003144666643 1104 14 1.3985564792556673 1104 15 1.376932156629664 1104 16 1.436125536718606 1104 17 1.4395606773304124 1104 18 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1107 19 0.8407813203659487 1107 20 0.8324542251285152 1107 21 0.88496380532019 1107 22 0.9116193286136962 1107 23 0.7083162570772927 1107 24 0.7328595526547659 1107 25 0.6611085023483696 1108 1 0.5076777704879767 1108 2 0.653616996328747 1108 3 0.5633126470469456 1108 4 0.6401856802662067 1108 5 0.5575229608703935 1108 6 0.5482799793997941 1108 7 0.5030213050652677 1108 8 0.5783015837800889 1108 9 0.7079453095370027 1108 10 0.6953027368253544 1108 11 0.5847054330667234 1108 12 0.6659704534390158 1108 13 0.657250471931043 1108 14 0.7682358997494205 1108 15 0.791291236704943 1108 16 0.7528991907341691 1108 17 0.7105527428043317 1108 18 0.7171322341336293 1108 19 0.8192317191121177 1108 20 0.806232312730522 1108 21 0.7517151829172732 1108 22 0.8123201335067947 1108 23 0.7257579949365578 1108 24 0.7222472108013989 1108 25 0.7927451501510347 1109 1 0.39664477322917335 1109 2 0.5676216501970508 1109 3 0.4843913029193665 1109 4 0.577871532892182 1109 5 0.5437936102871888 1109 6 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-3.3368171076597903 2216 24 -3.37496847332595 2216 25 -3.675470766917879 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136677.0 3 8.0 4 997.0 5 5683.0 6 7438.0 7 6908.0 8 6195.0 9 5616.0 10 5116.0 11 5005.0 12 4775.0 13 4505.0 14 4690.0 15 4682.0 16 4932.0 17 5059.0 18 5874.0 19 7169.0 20 9367.0 21 12297.0 22 15009.0 23 17629.0 24 20693.0 25 23618.0 26 26298.0 27 29935.0 28 33619.0 29 40316.0 30 49754.0 31 63203.0 32 80872.0 33 105567.0 34 141923.0 35 196498.0 36 287527.0 37 499150.0 38 1429288.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.939046746104495 17.87594181475151 12.548017660774068 25.636993778369927 2 15.795459741558272 20.61525470746213 39.06969513143094 24.519590419548656 3 18.566235486267768 24.730884466072403 29.15304714060839 27.549832907051442 4 12.5887700172768 16.849761338625942 38.174751230507184 32.38671741359006 5 14.772786876847244 36.11544480808194 34.25938914592896 14.852379169141855 6 31.659906805401384 37.20489393625196 17.38393369033533 13.75126556801132 7 28.07340216273031 31.244355339768514 22.23519547262029 18.447047024880884 8 25.722240402277695 34.156061323682216 20.569887301615864 19.551810972424228 9 26.702695911687996 15.448379918966218 19.423682514919776 38.42524165442601 10 15.229353210469283 27.54888227699967 32.89600544454317 24.325759067987878 11 34.8542591768642 22.681261536932055 23.516906703977227 18.947572582226517 12 23.76769631970225 24.559370369206963 29.94317476572895 21.729758545361836 13 28.86702884562015 21.237117426370265 25.228435622355555 24.66741810565403 14 22.77449919769762 20.643098506750718 26.634578637986095 29.94782365756557 15 24.134771806910113 27.811034736001627 23.484085559370584 24.570107897717673 16 24.112966038582844 27.26126339326285 24.71420285117079 23.911567716983516 17 23.633636500325878 26.403373641398414 25.91658429482947 24.046405563446243 18 24.35114021873827 25.924165976100987 26.55218678792563 23.172507017235116 19 25.309303395955013 25.897058388441085 25.96358115121286 22.830057064391045 20 24.633740269951193 25.850432875281843 26.10844709900213 23.407379755764836 21 24.72095087899825 25.74217657551931 25.888536501384763 23.648336044097675 22 24.07922982089383 25.69097795592894 26.29684372725792 23.932948495919312 23 23.66030720360305 25.488121676194986 26.20205928762384 24.64951183257812 24 23.604816563846125 26.095657870498577 26.34406798477028 23.955457580885017 25 23.971245314553933 25.685577213406248 26.19650051696215 24.146676955077666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15695.0 1 15695.0 2 12732.5 3 9770.0 4 9770.0 5 9770.0 6 7355.5 7 4941.0 8 4941.0 9 4941.0 10 6977.5 11 9014.0 12 9014.0 13 9014.0 14 13314.0 15 17614.0 16 17614.0 17 17614.0 18 29057.0 19 40500.0 20 40500.0 21 40500.0 22 58617.5 23 76735.0 24 76735.0 25 76735.0 26 103519.5 27 130304.0 28 130304.0 29 130304.0 30 157907.5 31 185511.0 32 185511.0 33 185511.0 34 216977.5 35 248444.0 36 248444.0 37 248444.0 38 277348.5 39 306253.0 40 306253.0 41 306253.0 42 335074.5 43 363896.0 44 363896.0 45 363896.0 46 382365.5 47 400835.0 48 400835.0 49 400835.0 50 403372.5 51 405910.0 52 405910.0 53 405910.0 54 377343.5 55 348777.0 56 348777.0 57 348777.0 58 311608.0 59 274439.0 60 274439.0 61 274439.0 62 235102.5 63 195766.0 64 195766.0 65 195766.0 66 159636.5 67 123507.0 68 123507.0 69 123507.0 70 95640.5 71 67774.0 72 67774.0 73 67774.0 74 52662.0 75 37550.0 76 37550.0 77 37550.0 78 29842.5 79 22135.0 80 22135.0 81 22135.0 82 16613.5 83 11092.0 84 11092.0 85 11092.0 86 8126.0 87 5160.0 88 5160.0 89 5160.0 90 3578.5 91 1997.0 92 1997.0 93 1997.0 94 1117.0 95 237.0 96 237.0 97 237.0 98 136.5 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40667188879055366 2 0.7101321713905903 3 0.7258409173181206 4 0.7370095632665958 5 0.7469977832205168 6 0.7485716845465893 7 0.7673071637934896 8 0.755563438514334 9 0.7421247425763311 10 0.7348303152766494 11 0.7490862292108822 12 0.7533841905243875 13 0.7521432298634458 14 0.762827598480822 15 0.7580756271694111 16 0.7511746752012475 17 0.7585296371673166 18 0.7621011824841731 19 0.7662175397985164 20 0.756774131842082 21 0.7563503891773702 22 0.7650976484703496 23 0.7534749925239688 24 0.7578940231702489 25 0.7603759444921323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3303892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.85031047370457 #Duplication Level Percentage of deduplicated Percentage of total 1 88.59450397863843 59.22570097235834 2 6.8999604123315645 9.225289916412713 3 1.6668328084241817 3.342848722527404 4 0.7509718335111506 2.0081080090891037 5 0.45011793058370503 1.504526170465104 6 0.28320789696248383 1.1359521504328185 7 0.2070229404865523 0.9687683492693699 8 0.15366954874695132 0.821828563527022 9 0.12220280909268386 0.7352366155744287 >10 0.7704216504112035 9.714267291915814 >50 0.05819152984186511 2.6677997860685405 >100 0.03803333785406589 5.066697208007666 >500 0.003636129433871302 1.6598166862863428 >1k 0.001090838827963173 1.1930796474704333 >5k 9.090323566359775E-5 0.3873725978098241 >10k+ 4.5451617831798874E-5 0.3427073127850817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNN 11279 0.3413852510917427 No Hit GTATCAACGCAGAGTACTTTTTTTT 7697 0.23296766359190918 No Hit GNNNNNNNNNNNNNNNNNNNNNNNN 5052 0.15291056729457259 No Hit TATCAACGCAGAGTACTTTTTTTTT 4311 0.13048247339804087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2106933277480015E-4 2 0.0 0.0 0.0 0.0 1.2106933277480015E-4 3 0.0 0.0 0.0 0.0 1.2106933277480015E-4 4 0.0 0.0 0.0 0.0 1.2106933277480015E-4 5 0.0 3.0267333193700037E-5 0.0 0.0 1.5133666596850017E-4 6 0.0 3.0267333193700037E-5 0.0 0.0 1.5133666596850017E-4 7 0.0 3.0267333193700037E-5 0.0 0.0 1.5133666596850017E-4 8 0.0 3.0267333193700037E-5 0.0 0.0 1.5133666596850017E-4 9 0.0 3.0267333193700037E-5 0.0 3.0267333193700037E-5 1.5133666596850017E-4 10 0.0 3.0267333193700037E-5 0.0 1.2106933277480015E-4 1.5133666596850017E-4 11 0.0 3.0267333193700037E-5 0.0 1.2106933277480015E-4 1.5133666596850017E-4 12 0.0 3.0267333193700037E-5 0.0 1.2106933277480015E-4 1.816039991622002E-4 13 0.0 3.0267333193700037E-5 0.0 1.2106933277480015E-4 2.1187133235590027E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1400 0.0 14.394752 1 GTATCAA 4560 0.0 13.508481 1 CGTCGTA 215 0.0 11.487874 10 CTAGACA 270 0.0 10.907267 4 CCGGTAG 105 3.4725072E-6 10.856341 9 CGAGCCG 325 0.0 10.815081 15 ACCGTCC 155 3.6725396E-9 10.418586 8 CCTATTC 585 0.0 10.393029 3 AGGCCCG 380 0.0 9.999566 10 GCGTTAT 135 6.3940206E-7 9.858041 1 CGAACGA 145 1.6195372E-7 9.827311 16 TACCGTC 305 0.0 9.654731 7 CGGTTCT 345 0.0 9.637263 12 TATCAAC 6425 0.0 9.552648 2 GTTCTAA 280 0.0 9.505968 1 TAGATCG 80 0.0045181853 9.499878 5 AAGGCCG 130 4.2427637E-6 9.499878 5 ACGCTTA 80 0.004518754 9.499733 17 ACCGTCG 300 0.0 9.499298 8 ATACCGT 280 0.0 9.498863 6 >>END_MODULE