FastQCFastQC Report
Thu 2 Feb 2017
SRR4050240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3303892
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT132740.4017685808131743No Hit
TATCAACGCAGAGTACTTTTTTTTT84490.25572869815357163No Hit
GGTATCAACGCAGAGTACTTTTTTT48240.14600961532640896No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38960.11792153012265534No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37630.11389597480789324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.015.6714981
GTATCAA52600.013.16970351
CGAACGA1302.6502676E-911.68353316
CGGTTCT3300.010.93184712
TAACGCT700.001476701210.8707154
TAGATCG1053.417921E-610.8707145
CGTCGCG803.7972326E-410.68012612
CGCGGTT3350.010.48561610
TAGACCG1002.3677474E-510.4630625
AGGACCG4600.010.3389955
CGAGCCG4150.010.29378715
CCGATAA1502.473098E-810.1266499
AATTCCG1606.0554157E-910.1063685
CGACCAT2800.09.83279510
AGGCCCG4450.09.60040510
CGGACAT2106.548362E-119.5118755
TAACCGT1106.862039E-59.4973276
CGGTCCA3100.09.49373310
GATAACG1606.713708E-89.49344511
ACGAACG1606.716982E-89.49315815
Produced by FastQC (version 0.11.5)