##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050240_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3303892 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.910600285965764 31.0 31.0 34.0 27.0 34.0 2 30.975638428859053 31.0 31.0 34.0 27.0 34.0 3 31.135311626409095 33.0 31.0 34.0 27.0 34.0 4 34.706792473846 37.0 35.0 37.0 32.0 37.0 5 34.61591450325858 37.0 35.0 37.0 32.0 37.0 6 34.637822907044175 37.0 35.0 37.0 32.0 37.0 7 34.54359585603888 37.0 35.0 37.0 31.0 37.0 8 34.51717913297408 37.0 35.0 37.0 31.0 37.0 9 36.134004682961795 39.0 37.0 39.0 32.0 39.0 10 36.02530500391659 39.0 37.0 39.0 31.0 39.0 11 36.023156931279836 39.0 37.0 39.0 31.0 39.0 12 35.938056691925766 39.0 37.0 39.0 31.0 39.0 13 35.93508807188613 39.0 37.0 39.0 31.0 39.0 14 37.12873604827276 40.0 37.0 41.0 31.0 41.0 15 37.10455729182431 40.0 37.0 41.0 31.0 41.0 16 37.07260527886505 40.0 37.0 41.0 31.0 41.0 17 37.06312343139546 40.0 37.0 41.0 31.0 41.0 18 37.03957332745743 40.0 37.0 41.0 31.0 41.0 19 37.075031205620526 40.0 37.0 41.0 31.0 41.0 20 37.054696400487664 40.0 37.0 41.0 31.0 41.0 21 36.98640996739603 40.0 37.0 41.0 31.0 41.0 22 36.926691913658196 40.0 37.0 41.0 31.0 41.0 23 36.84527581410046 40.0 37.0 41.0 31.0 41.0 24 36.784712091073196 40.0 37.0 41.0 31.0 41.0 25 36.50682255957519 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9410212273190623 1101 2 0.9690466526629535 1101 3 0.9727467765064794 1101 4 0.911805137621613 1101 5 0.9206697501470344 1101 6 0.8425286418655631 1101 7 0.948239504038284 1101 8 0.878800509999607 1101 9 1.0789505425536206 1101 10 1.033320567098258 1101 11 1.0228526529216566 1101 12 1.062248879360169 1101 13 1.1041987049337578 1101 14 1.2807032844852628 1101 15 1.3483841248167465 1101 16 1.370700359824184 1101 17 1.3288547483153792 1101 18 1.3222250087205012 1101 19 1.2679273564105173 1101 20 1.4122803140164564 1101 21 1.322647786773146 1101 22 1.3569591102225544 1101 23 1.400215432821554 1101 24 1.4306331292823486 1101 25 1.3654613714461732 1102 1 0.8663685446304505 1102 2 0.924080569095235 1102 3 0.9144744930299957 1102 4 0.7477998816616775 1102 5 0.8977324144349765 1102 6 0.8230877884525327 1102 7 0.9940129425214792 1102 8 0.9230399845959383 1102 9 0.9999847198815459 1102 10 1.0209356026306509 1102 11 1.075888365938134 1102 12 1.156791613772178 1102 13 1.041615685560167 1102 14 1.3159929727108022 1102 15 1.2870242627736772 1102 16 1.3151265587629553 1102 17 1.317093644258577 1102 18 1.234968119340671 1102 19 1.2959680448405066 1102 20 1.269637090048839 1102 21 1.3193418544496396 1102 22 1.2672129984664053 1102 23 1.4161471563507817 1102 24 1.4873473034828635 1102 25 1.4312465815905 1103 1 0.7374801093941628 1103 2 0.8040902213250973 1103 3 0.7574542671985363 1103 4 0.6897416694994192 1103 5 0.7584037646793718 1103 6 0.7540652774011463 1103 7 0.8050658853491086 1103 8 0.8154717791798376 1103 9 0.9690856924626203 1103 10 0.9426407076841272 1103 11 0.9687820603514083 1103 12 0.9884468151125887 1103 13 1.028357396497185 1103 14 1.1680667528099136 1103 15 1.207844365375692 1103 16 1.1220507454027953 1103 17 1.1503954228603064 1103 18 1.2149130352342183 1103 19 1.1167735055845966 1103 20 1.1831425564663434 1103 21 1.104617001221257 1103 22 1.1544177736445178 1103 23 1.2463398565794321 1103 24 1.3041089853195018 1103 25 1.2673733716567313 1104 1 0.6795664899491669 1104 2 0.7269131698370224 1104 3 0.715212140849907 1104 4 0.539367867804728 1104 5 0.5979000019034402 1104 6 0.5829674994609348 1104 7 0.6675063901022469 1104 8 0.6765220080825358 1104 9 0.7820740191915903 1104 10 0.7904649236201848 1104 11 0.7578602834109844 1104 12 0.767318677943102 1104 13 0.7975459031414402 1104 14 0.9304896904750493 1104 15 0.927297262589363 1104 16 0.9112878975538194 1104 17 0.9089682207550851 1104 18 0.899632144571072 1104 19 0.8499610360175112 1104 20 0.9025789476912891 1104 21 0.9220433167190834 1104 22 0.9531897280832737 1104 23 0.9669333176140995 1104 24 0.96868300701545 1104 25 0.9055406409884839 1105 1 0.6106002504677335 1105 2 0.6556016827470152 1105 3 0.564594298411663 1105 4 0.5057787993556389 1105 5 0.509363116039232 1105 6 0.5876192261423014 1105 7 0.6453816345826482 1105 8 0.698809120982439 1105 9 0.8196534873388543 1105 10 0.8146879752468053 1105 11 0.7301869225747595 1105 12 0.8606174437963006 1105 13 0.8685413610704487 1105 14 0.9152672723995892 1105 15 0.8845584577957268 1105 16 0.9141637810138832 1105 17 0.8828515726998774 1105 18 0.9494763126998507 1105 19 0.9522044766368509 1105 20 1.053251075127129 1105 21 1.0846374248516355 1105 22 1.0102209132310804 1105 23 1.116901911481115 1105 24 1.0629858191366566 1105 25 0.9925143451094627 1106 1 0.5099760998494922 1106 2 0.5542476692988103 1106 3 0.5001847671203024 1106 4 0.4067553410688163 1106 5 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0.43914377672426497 1107 18 0.4127353456918357 1107 19 0.5015212466864085 1107 20 0.5843630038268515 1107 21 0.4730678740762073 1107 22 0.5109257446292546 1107 23 0.41448571692528446 1107 24 0.4597510986778488 1107 25 0.417927396042721 1108 1 0.4099273424617671 1108 2 0.36030023593257 1108 3 0.33270762740414384 1108 4 0.2725571817503436 1108 5 0.23932679269739054 1108 6 0.23073213508585866 1108 7 0.27870377352570586 1108 8 0.29713307391455146 1108 9 0.3772924763422836 1108 10 0.32035971437588273 1108 11 0.44389107579035425 1108 12 0.4036736202060638 1108 13 0.3076276499060455 1108 14 0.4689731514458302 1108 15 0.4574725829935389 1108 16 0.47872223076991105 1108 17 0.4501617681481278 1108 18 0.4951700229805951 1108 19 0.46274596222440323 1108 20 0.4639693546399215 1108 21 0.4629897004900201 1108 22 0.44056748782025323 1108 23 0.5243712318336549 1108 24 0.4853359482731747 1108 25 0.43761888750816524 1109 1 0.2910932645693194 1109 2 0.2662412392079183 1109 3 0.24646486912523713 1109 4 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-1.595896999866369 2216 18 -1.7865792483075609 2216 19 -1.752614120403436 2216 20 -1.8432733137753772 2216 21 -1.8600403152780842 2216 22 -1.8329341941215276 2216 23 -1.798900375764454 2216 24 -1.898666409004754 2216 25 -1.7914369928732654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42377.0 3 4.0 4 846.0 5 6181.0 6 7680.0 7 5953.0 8 5035.0 9 4397.0 10 4019.0 11 3806.0 12 3493.0 13 3297.0 14 3295.0 15 3482.0 16 3562.0 17 3627.0 18 4233.0 19 5161.0 20 6866.0 21 9173.0 22 11749.0 23 14482.0 24 17750.0 25 20457.0 26 22411.0 27 25590.0 28 29746.0 29 36375.0 30 46748.0 31 61464.0 32 79608.0 33 105316.0 34 143067.0 35 197444.0 36 285578.0 37 492652.0 38 1586968.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37681665244345 18.513490876112687 13.09348742848995 26.016205042953917 2 15.912482376066443 21.247668555633513 37.944805684214224 24.89504338408582 3 18.247146093153166 25.08344703761503 29.33134618201806 27.338060687213744 4 12.858717258050017 17.418534266050923 37.537453639817286 32.18529483608177 5 14.550808192954893 36.40000169782066 34.207933309604265 14.841256799620172 6 31.544408804637232 37.656348079492815 17.09811423935961 13.701128876510339 7 27.853109242781816 31.47213714453138 22.20632154736166 18.46843206532514 8 25.772334346283763 34.37742896231098 20.37343971330678 19.47679697809848 9 26.639623739702646 15.32859971130939 19.39551128172517 38.63626526726279 10 15.149136815290943 27.797102256423788 32.83264907325235 24.221111855032916 11 34.91876744934997 22.77801521135355 23.367146430465326 18.936070908831155 12 23.74675335878484 24.69420809195717 29.92881830886201 21.630220240395982 13 28.907194251790493 21.296380096661114 25.27173263246408 24.52469301908432 14 22.75146177233766 20.58871072224691 26.80404319750666 29.855784307908767 15 24.0116242642138 27.908374016542876 23.650704238994194 24.429297480249133 16 23.959294959299196 27.32849940039941 24.846437476580466 23.865768163720926 17 23.604427377675318 26.304157916180586 26.070293671256977 24.021121034887123 18 24.24729363493446 25.826044424158518 26.870211309255364 23.056450631651654 19 25.142507594906444 25.719196854345178 26.369587806259286 22.7687077444891 20 24.511045239458053 25.69782467156073 26.490988521765242 23.300141567215977 21 24.584965689124793 25.52077736811024 26.35140595738859 23.542850985376372 22 23.90049421159811 25.5659158653788 26.69381279041893 23.839777132604166 23 23.61074705508099 25.209408727941486 26.65146409042123 24.5283801265563 24 23.474423391246443 25.953992372419638 26.736848477510744 23.83473575882317 25 23.86557567399531 25.47727397932837 26.652910198331668 24.004240148344653 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 589.0 1 589.0 2 1077.5 3 1566.0 4 1566.0 5 1566.0 6 3871.0 7 6176.0 8 6176.0 9 6176.0 10 8108.0 11 10040.0 12 10040.0 13 10040.0 14 14018.0 15 17996.0 16 17996.0 17 17996.0 18 30375.5 19 42755.0 20 42755.0 21 42755.0 22 61439.0 23 80123.0 24 80123.0 25 80123.0 26 108246.5 27 136370.0 28 136370.0 29 136370.0 30 164149.5 31 191929.0 32 191929.0 33 191929.0 34 221312.5 35 250696.0 36 250696.0 37 250696.0 38 278934.0 39 307172.0 40 307172.0 41 307172.0 42 335872.0 43 364572.0 44 364572.0 45 364572.0 46 382818.0 47 401064.0 48 401064.0 49 401064.0 50 403631.0 51 406198.0 52 406198.0 53 406198.0 54 377937.5 55 349677.0 56 349677.0 57 349677.0 58 312334.0 59 274991.0 60 274991.0 61 274991.0 62 235244.5 63 195498.0 64 195498.0 65 195498.0 66 158874.0 67 122250.0 68 122250.0 69 122250.0 70 94287.5 71 66325.0 72 66325.0 73 66325.0 74 51974.5 75 37624.0 76 37624.0 77 37624.0 78 29766.5 79 21909.0 80 21909.0 81 21909.0 82 16477.0 83 11045.0 84 11045.0 85 11045.0 86 8040.5 87 5036.0 88 5036.0 89 5036.0 90 3546.5 91 2057.0 92 2057.0 93 2057.0 94 1127.5 95 198.0 96 198.0 97 198.0 98 117.0 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4439915106183859 2 0.004419030646280205 3 0.0 4 0.004267693980311705 5 0.1678626298922604 6 0.04252560313714855 7 0.0014830993264913018 8 3.632079983244004E-4 9 2.7240599874330033E-4 10 0.0024213866554960027 11 0.0013317626605228016 12 0.0028148619870141034 13 0.005599456640834506 14 0.011138378615281613 15 0.003420208650888104 16 7.869506630362008E-4 17 0.00805111062952421 18 4.2374266471180053E-4 19 9.988219953921012E-4 20 0.004873040644185706 21 0.003904485981987305 22 0.0013317626605228016 23 0.0019976439907842024 24 0.0027845946538204032 25 0.007082555967325808 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3303892.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.16518266031134 #Duplication Level Percentage of deduplicated Percentage of total 1 88.11520945273294 58.30158928591668 2 7.238405387638094 9.578608292849127 3 1.7552817566046601 3.484156141381785 4 0.7531740501701997 1.9933559441807107 5 0.45554568683385177 1.5070631789739397 6 0.29543671181233194 1.1728574400974825 7 0.21574825533122516 0.9992515905843803 8 0.15494871382279574 0.8201767962452472 9 0.11990663859898852 0.7140280180577413 >10 0.7894806105536514 9.745128992339664 >50 0.06201320583145071 2.8106615340013503 >100 0.0397642502695947 5.235747099135966 >500 0.0038483198506079545 1.719414490646814 >1k 0.001145333286583268 1.2594833132099712 >5k 4.5813331463330724E-5 0.25611009658991685 >10k+ 4.5813331463330724E-5 0.40236778578939 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 13274 0.4017685808131743 No Hit TATCAACGCAGAGTACTTTTTTTTT 8449 0.25572869815357163 No Hit GGTATCAACGCAGAGTACTTTTTTT 4824 0.14600961532640896 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3896 0.11792153012265534 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3763 0.11389597480789324 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.0267333193700037E-5 0.0 0.0 0.0 0.0 2 3.0267333193700037E-5 0.0 0.0 0.0 0.0 3 3.0267333193700037E-5 0.0 0.0 0.0 0.0 4 3.0267333193700037E-5 0.0 0.0 0.0 0.0 5 3.0267333193700037E-5 0.0 0.0 0.0 0.0 6 6.0534666387400075E-5 0.0 0.0 0.0 0.0 7 1.2106933277480015E-4 0.0 0.0 3.0267333193700037E-5 0.0 8 1.2106933277480015E-4 0.0 0.0 3.0267333193700037E-5 0.0 9 1.2106933277480015E-4 0.0 0.0 6.0534666387400075E-5 0.0 10 1.2106933277480015E-4 0.0 0.0 9.08019995811001E-5 0.0 11 1.2106933277480015E-4 0.0 0.0 1.2106933277480015E-4 0.0 12 1.2106933277480015E-4 0.0 0.0 1.2106933277480015E-4 6.0534666387400075E-5 13 1.2106933277480015E-4 0.0 0.0 1.2106933277480015E-4 9.08019995811001E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1650 0.0 15.671498 1 GTATCAA 5260 0.0 13.1697035 1 CGAACGA 130 2.6502676E-9 11.683533 16 CGGTTCT 330 0.0 10.931847 12 TAACGCT 70 0.0014767012 10.870715 4 TAGATCG 105 3.417921E-6 10.870714 5 CGTCGCG 80 3.7972326E-4 10.680126 12 CGCGGTT 335 0.0 10.485616 10 TAGACCG 100 2.3677474E-5 10.463062 5 AGGACCG 460 0.0 10.338995 5 CGAGCCG 415 0.0 10.293787 15 CCGATAA 150 2.473098E-8 10.126649 9 AATTCCG 160 6.0554157E-9 10.106368 5 CGACCAT 280 0.0 9.832795 10 AGGCCCG 445 0.0 9.600405 10 CGGACAT 210 6.548362E-11 9.511875 5 TAACCGT 110 6.862039E-5 9.497327 6 CGGTCCA 310 0.0 9.493733 10 GATAACG 160 6.713708E-8 9.493445 11 ACGAACG 160 6.716982E-8 9.493158 15 >>END_MODULE