FastQCFastQC Report
Thu 2 Feb 2017
SRR4050239_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050239_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559496
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145130.40772626236972875No Hit
TATCAACGCAGAGTACTTTTTTTTT85360.2398092314192796No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN82680.23228007560620942No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN62430.17538999903357105No Hit
GGTATCAACGCAGAGTACTTTTTTT57080.16035978127240486No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47220.13265923040790045No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41880.1176571065117084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28100.014.099841
GTATCAA85850.012.8492081
CGAACGA2450.012.7960816
AACCGGT853.948644E-612.29375214
CGCAGAT2400.012.2699478
ATTACGG1353.7289283E-1011.96226813
AACCGCG2850.011.6658247
CTCGACG1251.8360879E-811.39933813
CGACCGG600.005880711611.08268910
CCGTCGT3650.010.6707649
CGCGCTA2950.010.62725416
CGACCGT909.520805E-510.5556919
GATTACG1355.696529E-810.5553912
GCGTTAT2900.010.4842261
CGTCGTA3550.010.43601310
ACGCTTA1303.8452163E-710.23089817
CGAGCCG7050.010.10622515
CCGTCCA1606.186383E-910.0933079
CGCTTAC1356.447426E-79.85211618
GTATTAG5500.09.8468331
Produced by FastQC (version 0.11.5)