##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050239_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3559496
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.02486925115241	31.0	31.0	34.0	26.0	34.0
2	30.120290906353034	31.0	31.0	34.0	26.0	34.0
3	30.241574088016954	31.0	31.0	34.0	26.0	34.0
4	33.59748037362593	37.0	35.0	37.0	28.0	37.0
5	33.69934591863567	37.0	35.0	37.0	28.0	37.0
6	33.76817111186528	37.0	35.0	37.0	29.0	37.0
7	33.64310003438689	37.0	35.0	37.0	29.0	37.0
8	33.57525250765839	37.0	35.0	37.0	28.0	37.0
9	35.02879593065985	39.0	35.0	39.0	28.0	39.0
10	34.94336951073972	39.0	35.0	39.0	27.0	39.0
11	34.96946168783446	39.0	35.0	39.0	27.0	39.0
12	34.863522813342115	39.0	35.0	39.0	27.0	39.0
13	34.841881547275236	39.0	35.0	39.0	27.0	39.0
14	35.696764654321846	40.0	36.0	41.0	26.0	41.0
15	35.83027934291821	40.0	36.0	41.0	27.0	41.0
16	35.86594169511639	40.0	36.0	41.0	27.0	41.0
17	35.87262859685753	40.0	36.0	41.0	27.0	41.0
18	35.700396348247054	40.0	36.0	41.0	27.0	41.0
19	35.84597594715656	40.0	36.0	41.0	27.0	41.0
20	35.815887979646554	40.0	36.0	41.0	27.0	41.0
21	35.78250713022293	40.0	36.0	41.0	27.0	41.0
22	35.71597804857766	40.0	36.0	41.0	27.0	41.0
23	35.61444288742002	40.0	36.0	41.0	27.0	41.0
24	35.561326940668	40.0	36.0	41.0	27.0	41.0
25	35.28872093127791	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1673591727284531
1101	2	1.2175749335866648
1101	3	1.1033478746073762
1101	4	1.1894917638109774
1101	5	1.2044757429463502
1101	6	1.186054293860657
1101	7	1.2121991805094368
1101	8	1.1955477620405475
1101	9	1.3304720835354829
1101	10	1.4071556132947052
1101	11	1.3667566302647387
1101	12	1.4036659561424827
1101	13	1.4061152534718389
1101	14	1.8692214575772184
1101	15	1.7735559679050823
1101	16	1.7715465488627586
1101	17	1.743843328848513
1101	18	1.8581829085164543
1101	19	1.716043545483032
1101	20	1.6475230864674018
1101	21	1.643043293766418
1101	22	1.6651217858230893
1101	23	1.656323016639874
1101	24	1.7106335161257746
1101	25	1.8123694190331676
1102	1	0.9409837905668574
1102	2	0.9839815588519656
1102	3	0.8764355570983291
1102	4	0.9993607093379993
1102	5	0.9385953040399073
1102	6	0.8891431138750079
1102	7	0.992546963567932
1102	8	0.849158940839132
1102	9	1.050283198569197
1102	10	1.080535070070738
1102	11	1.0139361283729613
1102	12	1.0964151370929258
1102	13	1.0864609895490247
1102	14	1.4813748622819674
1102	15	1.3965973035613146
1102	16	1.3230068176566405
1102	17	1.3327626545111784
1102	18	1.4271005162103378
1102	19	1.2899795755413166
1102	20	1.304527347086072
1102	21	1.2635905369265856
1102	22	1.276870187331653
1102	23	1.3291850731868422
1102	24	1.4243914550717065
1102	25	1.4053114835736409
1103	1	0.7066277508155281
1103	2	0.7284607823385443
1103	3	0.697469999064495
1103	4	0.7903239361875052
1103	5	0.7012278877331966
1103	6	0.6542289564180166
1103	7	0.6554081252238504
1103	8	0.7723182963102246
1103	9	0.8549593906203512
1103	10	0.904843173175756
1103	11	0.8084184827637415
1103	12	0.8404562535496467
1103	13	0.886851750984043
1103	14	1.2179159482200603
1103	15	1.1209634315865813
1103	16	1.0882398961293944
1103	17	1.090418856990489
1103	18	1.1655984392308412
1103	19	1.0914285650321034
1103	20	1.1299049635537273
1103	21	1.1005597443739745
1103	22	1.1241514427355312
1103	23	0.9861417798515077
1103	24	1.0532130573797787
1103	25	1.195458655392308
1104	1	0.7238323574090515
1104	2	0.7060153531247906
1104	3	0.6769381405588959
1104	4	0.7695387682090171
1104	5	0.6934214862896084
1104	6	0.6514628236203848
1104	7	0.6350321239078696
1104	8	0.6850383532030833
1104	9	0.7372105300560392
1104	10	0.8353147491777406
1104	11	0.8088229138933087
1104	12	0.8679564072467727
1104	13	0.8504566879017617
1104	14	1.2173908856196363
1104	15	1.06252213641001
1104	16	1.035069332202191
1104	17	0.9610158498439887
1104	18	1.074863634060634
1104	19	1.0013681994489616
1104	20	0.9865293095101677
1104	21	0.9459452582956089
1104	22	1.0860904690102657
1104	23	1.0376515009867688
1104	24	1.0490962176584588
1104	25	1.119252963780184
1105	1	0.6292044339505125
1105	2	0.6477422607553116
1105	3	0.6366452041072073
1105	4	0.7076275941008205
1105	5	0.7332679308915431
1105	6	0.6711155521816821
1105	7	0.6192253674703565
1105	8	0.719914327689267
1105	9	0.8587767951162846
1105	10	0.7544524765578444
1105	11	0.6769542628184197
1105	12	0.8258751410053335
1105	13	0.7593983368504951
1105	14	1.130573025945651
1105	15	0.9787635727435813
1105	16	0.9591397314462284
1105	17	0.8992913046042048
1105	18	1.060171926052135
1105	19	0.8826840510908198
1105	20	0.9782680290943873
1105	21	1.0258743865734274
1105	22	0.955133700760797
1105	23	0.9521636672990823
1105	24	0.9732540253598501
1105	25	0.9234627440778453
1106	1	0.5932337725935994
1106	2	0.518063545895874
1106	3	0.5428302805474239
1106	4	0.7309271789950174
1106	5	0.5792409546391966
1106	6	0.5649740112279531
1106	7	0.5010390085894514
1106	8	0.5780102407532866
1106	9	0.7299338041046184
1106	10	0.6272443724326635
1106	11	0.7514123191333155
1106	12	0.7134335539287378
1106	13	0.7132889694087439
1106	14	1.0147708817948171
1106	15	0.9112886309596391
1106	16	0.8480163584022335
1106	17	0.8593711759101552
1106	18	0.9619548560916158
1106	19	0.8266502801527267
1106	20	0.7807729507417562
1106	21	0.9016346826597328
1106	22	0.9138873709035522
1106	23	0.8547771464256684
1106	24	0.8914083232235726
1106	25	0.887621676727214
1107	1	0.39544393500224473
1107	2	0.4453066518530875
1107	3	0.4055110970510327
1107	4	0.44896028021352663
1107	5	0.38127522683317494
1107	6	0.38643497027150175
1107	7	0.4058868857911122
1107	8	0.391829659463383
1107	9	0.4328403109952532
1107	10	0.4981242768608638
1107	11	0.44566555828934895
1107	12	0.4253578057891758
1107	13	0.31509167194897714
1107	14	0.7013627822082427
1107	15	0.5793835806867449
1107	16	0.42851016228923555
1107	17	0.4027889437198624
1107	18	0.47899192516955935
1107	19	0.530841531637428
1107	20	0.4901448218219784
1107	21	0.5046486389600489
1107	22	0.493271029210554
1107	23	0.4837321476544645
1107	24	0.5064857156353639
1107	25	0.4840275911719729
1108	1	0.33842352001530784
1108	2	0.21878503011711814
1108	3	0.2294971629232201
1108	4	0.46377319817197815
1108	5	0.2445077807281706
1108	6	0.33935786060349926
1108	7	0.28393153612142186
1108	8	0.29305512553057866
1108	9	0.333853755632191
1108	10	0.4482164380650602
1108	11	0.4253032172934397
1108	12	0.46571814226862784
1108	13	0.4200262602228051
1108	14	0.6888078548288377
1108	15	0.4741572147778683
1108	16	0.5453323957724052
1108	17	0.4935966200868478
1108	18	0.5350298327117855
1108	19	0.48773388485896874
1108	20	0.46786542234991657
1108	21	0.4255425670414539
1108	22	0.4193874825801487
1108	23	0.4969856435434892
1108	24	0.4877030267161899
1108	25	0.41548017963392425
1109	1	0.21661487074770847
1109	2	0.26963467189844437
1109	3	0.2704801761363669
1109	4	0.33059292720812294
1109	5	0.24944341261160474
1109	6	0.2897537394450609
1109	7	0.24914795321934946
1109	8	0.20140511708859776
1109	9	0.15758323123539952
1109	10	0.2111321397776038
1109	11	0.17061561785034485
1109	12	0.2411740617263618
1109	13	0.21900942912602517
1109	14	0.4702951539899516
1109	15	0.42628221997192384
1109	16	0.32014103110153513
1109	17	0.38550125544333014
1109	18	0.4864170523447271
1109	19	0.34640092400713485
1109	20	0.280873979255432
1109	21	0.377863565530312
1109	22	0.2814508731628962
1109	23	0.3578407594615953
1109	24	0.35739526398786836
1109	25	0.24845681086882365
1110	1	-0.24151957375041277
1110	2	-0.19796306482342985
1110	3	-0.22405653262580216
1110	4	-0.16579435353557415
1110	5	-0.25875691599555495
1110	6	-0.25890289515619713
1110	7	-0.2736548992446117
1110	8	-0.23725149039985638
1110	9	-0.2869547592228656
1110	10	-0.3142723092853288
1110	11	-0.27195466099743015
1110	12	-0.344625016635554
1110	13	-0.31176008888722606
1110	14	-0.14835743094459986
1110	15	-0.23383730065401664
1110	16	-0.32132346443766835
1110	17	-0.4054407394279167
1110	18	-0.24778062068671147
1110	19	-0.33272122015213057
1110	20	-0.43253895808638987
1110	21	-0.34101592890959154
1110	22	-0.38846715251243324
1110	23	-0.4036638802782804
1110	24	-0.46128214148458113
1110	25	-0.5536103677532722
1111	1	-0.33454409013829434
1111	2	-0.3584077886030279
1111	3	-0.4031819449162697
1111	4	-0.28105928045157214
1111	5	-0.34014476291253715
1111	6	-0.3727812404939215
1111	7	-0.3258715103725933
1111	8	-0.23038820991018127
1111	9	-0.5137207369666399
1111	10	-0.3032156805371713
1111	11	-0.2624259512612923
1111	12	-0.30119007007416343
1111	13	-0.28519450522216516
1111	14	-0.09913682443082905
1111	15	-0.29128559203585525
1111	16	-0.3628255047963407
1111	17	-0.4060417190297301
1111	18	-0.22711704284243694
1111	19	-0.37748105740075033
1111	20	-0.29159880016446493
1111	21	-0.3214465200715608
1111	22	-0.3971766567484494
1111	23	-0.34083480356014206
1111	24	-0.38296628704941327
1111	25	-0.4862176848558235
1112	1	-0.5900018254763353
1112	2	-0.6356551751421229
1112	3	-0.6791014022276336
1112	4	-0.565532285235534
1112	5	-0.5348994899229353
1112	6	-0.5937449304729157
1112	7	-0.5997357939172758
1112	8	-0.588021192672727
1112	9	-0.7149732048837194
1112	10	-0.6460433756291835
1112	11	-0.7023255260201964
1112	12	-0.6817153567322194
1112	13	-0.8025360522897742
1112	14	-0.6922399453967358
1112	15	-0.7815116912081521
1112	16	-0.936009212746832
1112	17	-0.8861555279749851
1112	18	-0.7453672896341388
1112	19	-0.7963940032078227
1112	20	-0.8431007491861564
1112	21	-0.8133167898971649
1112	22	-0.8473606431306067
1112	23	-0.9013506615093689
1112	24	-0.884934134519483
1112	25	-0.827953168441006
1113	1	-0.8385238718251067
1113	2	-0.9159258137011221
1113	3	-0.8531950623607116
1113	4	-0.7516165240063017
1113	5	-0.9047243584690605
1113	6	-0.8904000761250117
1113	7	-1.0339981030534702
1113	8	-1.0286227671111163
1113	9	-0.9993868213392147
1113	10	-1.167681751136044
1113	11	-1.0625899077357701
1113	12	-1.0453957343086557
1113	13	-1.0993957485922223
1113	14	-1.0335679002512066
1113	15	-1.090087928438777
1113	16	-1.0504988438337222
1113	17	-1.1916219184982708
1113	18	-1.1535891729440095
1113	19	-1.127898939496312
1113	20	-1.3772569476724854
1113	21	-1.20960081148327
1113	22	-1.2089949877864186
1113	23	-1.590853603429018
1113	24	-1.4083827832819509
1113	25	-1.5569463430671036
1114	1	-2.472551928168805
1114	2	-2.673542678752405
1114	3	-2.2886614291844083
1114	4	-5.5541871743597575
1114	5	-3.0442894324793386
1114	6	-2.4972553280853163
1114	7	-2.2283486834265993
1114	8	-2.4879997315514544
1114	9	-3.0403512816463376
1114	10	-2.7686034266545008
1114	11	-2.5253507017205905
1114	12	-2.7711544793534486
1114	13	-2.6316425475883847
1114	14	-2.527332689532031
1114	15	-3.2384141494091097
1114	16	-3.2454326506981204
1114	17	-2.959012825558503
1114	18	-2.9181822666887953
1114	19	-2.960209148742301
1114	20	-3.1607995553862125
1114	21	-3.1189045037060907
1114	22	-3.242995210378126
1114	23	-3.3599118437564073
1114	24	-3.3628077184866214
1114	25	-3.36453475279324
1115	1	-2.7764391195602798
1115	2	-2.1733688503557964
1115	3	-1.7617065686316842
1115	4	-1.7088462803651723
1115	5	-1.7312144877742455
1115	6	-1.7522937295943422
1115	7	-1.7604514865114638
1115	8	-1.7141883487630665
1115	9	-1.8924522551630503
1115	10	-1.8173312164351785
1115	11	-1.8336456568514947
1115	12	-2.2515495495412736
1115	13	-2.3585207488470985
1115	14	-2.5795996837341306
1115	15	-2.4567605810520163
1115	16	-2.305486185959836
1115	17	-2.3528111527660798
1115	18	-2.290269524271011
1115	19	-2.327546715331202
1115	20	-2.3368128486943434
1115	21	-2.1621567961044903
1115	22	-2.3944697607355323
1115	23	-2.3415001147873156
1115	24	-2.5576854714817117
1115	25	-2.670018834773437
1116	1	-1.397388674160748
1116	2	-1.3560052620350156
1116	3	-1.4676658401678147
1116	4	-1.3261431270294892
1116	5	-1.4217451254606033
1116	6	-1.5273964899716788
1116	7	-1.6541252188997504
1116	8	-1.6396391916970856
1116	9	-1.9277432207378595
1116	10	-2.3856362366920223
1116	11	-2.037628050487406
1116	12	-2.1075663735315118
1116	13	-1.9576425341947115
1116	14	-2.7858838064900624
1116	15	-2.6877538784699624
1116	16	-2.597963273846254
1116	17	-2.4372376874920434
1116	18	-2.3042307458370743
1116	19	-2.112515874301451
1116	20	-2.293063019377321
1116	21	-2.455560646417176
1116	22	-2.2727053563840585
1116	23	-2.7820261394202817
1116	24	-2.909095884877729
1116	25	-2.7180323248177274
1201	1	1.117000284994642
1201	2	1.1134871908380433
1201	3	1.0837979540716773
1201	4	1.1561061667290815
1201	5	1.144053665787304
1201	6	1.051278426907679
1201	7	1.0482347256612456
1201	8	1.1281739970303164
1201	9	1.291962240610097
1201	10	1.237307521496973
1201	11	1.1883938221695018
1201	12	1.2573029434023724
1201	13	1.300641128717956
1201	14	1.7079342142162588
1201	15	1.519352841616076
1201	16	1.498131949462362
1201	17	1.484783223313265
1201	18	1.602514143852936
1201	19	1.629092538491932
1201	20	1.5489452813262687
1201	21	1.4501116272636807
1201	22	1.4487827492227012
1201	23	1.5514040733231198
1201	24	1.6100881317236784
1201	25	1.545093730757607
1202	1	1.126238019662022
1202	2	1.0969473116951889
1202	3	1.0675375593273841
1202	4	1.1738677500857264
1202	5	1.1160207944131884
1202	6	1.073484530767523
1202	7	1.1178044530003888
1202	8	1.1479352269658918
1202	9	1.260208851365384
1202	10	1.3018429368314983
1202	11	1.2843019285312849
1202	12	1.2886179600442702
1202	13	1.3665256992031019
1202	14	1.6908188203460597
1202	15	1.5748272328180306
1202	16	1.5453873152838966
1202	17	1.5306771899958136
1202	18	1.6370889451197712
1202	19	1.5488939892201827
1202	20	1.530535381149285
1202	21	1.5664875220557732
1202	22	1.5638199837113405
1202	23	1.5938180526864656
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2211	10	-0.32331575137676083
2211	11	-0.31660682113258787
2211	12	-0.3820590702126623
2211	13	-0.3500080396411178
2211	14	-0.10434983059643343
2211	15	-0.18006981315769366
2211	16	-0.28494584916671073
2211	17	-0.40511691176545384
2211	18	-0.3425299348530899
2211	19	-0.30348781980230655
2211	20	-0.4130894066413049
2211	21	-0.4512915441420233
2211	22	-0.45128799928651375
2211	23	-0.3831819669331651
2211	24	-0.3818712177668928
2211	25	-0.4139627408614359
2212	1	-0.39079838870644323
2212	2	-0.39872646709244464
2212	3	-0.4324989534740311
2212	4	-0.3311760163340338
2212	5	-0.39809698412403094
2212	6	-0.4692084760063935
2212	7	-0.4362383661991345
2212	8	-0.5063776954180312
2212	9	-0.4344518433045721
2212	10	-0.610766648226047
2212	11	-0.6360822424756662
2212	12	-0.5978203263849622
2212	13	-0.5667654478192645
2212	14	-0.6750645898073415
2212	15	-0.6350473990523042
2212	16	-0.5870161305386787
2212	17	-0.822825880565297
2212	18	-0.5820425445553497
2212	19	-0.6598014587355507
2212	20	-0.747977965459171
2212	21	-0.7494954937523346
2212	22	-0.8691026903439862
2212	23	-0.921734710635441
2212	24	-0.8020637961904953
2212	25	-0.7911083243298904
2213	1	-1.0073279053414232
2213	2	-0.838781983123706
2213	3	-0.7951291974440977
2213	4	-0.7883503976719837
2213	5	-0.7967700045899022
2213	6	-0.8746700323353664
2213	7	-0.9438401583078857
2213	8	-0.9387856996092196
2213	9	-0.9233195402973848
2213	10	-1.0280041047304564
2213	11	-1.05622845818165
2213	12	-0.9922968959323413
2213	13	-1.1225197112136627
2213	14	-0.8767128456761668
2213	15	-1.128905889664523
2213	16	-1.1785885639045546
2213	17	-1.236177146818612
2213	18	-1.1384161774561932
2213	19	-1.083934702027328
2213	20	-1.097568387085147
2213	21	-1.1663562762149766
2213	22	-1.3218829513153025
2213	23	-1.2716215971667637
2213	24	-1.2319581968309947
2213	25	-1.3081283810904196
2214	1	-1.2370185811270211
2214	2	-1.1143394442105574
2214	3	-1.0389064791266627
2214	4	-0.9910840262359173
2214	5	-0.9801660010679853
2214	6	-1.1764599351024856
2214	7	-1.0768063508289742
2214	8	-1.109230829201067
2214	9	-1.116257335465761
2214	10	-1.2897131096391163
2214	11	-1.2466030419230805
2214	12	-1.2149752882706437
2214	13	-1.3630371184946881
2214	14	-1.1855800727801622
2214	15	-1.349521285048219
2214	16	-1.2713730702040564
2214	17	-1.3203305769763176
2214	18	-1.2748778675091899
2214	19	-1.4922091739809815
2214	20	-1.4079561200931963
2214	21	-1.4290666856816472
2214	22	-1.5286264257691045
2214	23	-1.5182756624112344
2214	24	-1.5708438207506106
2214	25	-1.623161684652537
2215	1	-1.854325599298921
2215	2	-1.8929497624882146
2215	3	-1.770086407713432
2215	4	-1.9149481493502876
2215	5	-1.8163402229880745
2215	6	-1.6026913525136308
2215	7	-1.7690186274334714
2215	8	-1.8365494242140343
2215	9	-2.2269812608794908
2215	10	-1.8406741509552802
2215	11	-2.0537564049669186
2215	12	-2.103421338283688
2215	13	-2.191644203779063
2215	14	-2.7895102530077693
2215	15	-2.529770966270121
2215	16	-2.257852593217393
2215	17	-2.383936065583171
2215	18	-2.1881631163642012
2215	19	-2.3199003931464617
2215	20	-2.565776904251379
2215	21	-2.405059394228914
2215	22	-2.6521553828599735
2215	23	-2.265193158156933
2215	24	-2.4853696967121763
2215	25	-2.429802847420241
2216	1	-1.756830299265424
2216	2	-1.6685978259294956
2216	3	-1.6905514731727145
2216	4	-1.5493209464281819
2216	5	-2.169905847842813
2216	6	-1.861953101950668
2216	7	-1.9777765950927169
2216	8	-2.3238147793148904
2216	9	-2.3737884704819407
2216	10	-2.3312456563390995
2216	11	-2.240439026520299
2216	12	-2.4025128298467493
2216	13	-2.368542987849928
2216	14	-2.2890952949660246
2216	15	-2.276455303437068
2216	16	-2.4444006493265817
2216	17	-2.5718832093501547
2216	18	-2.7405756859651547
2216	19	-2.5252325488724168
2216	20	-2.650259514974344
2216	21	-2.725552829610905
2216	22	-2.9172195961672855
2216	23	-2.7126739617349998
2216	24	-2.8818927518269817
2216	25	-2.879106849831686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128293.0
3	5.0
4	824.0
5	5501.0
6	8186.0
7	8090.0
8	7102.0
9	6240.0
10	5846.0
11	5827.0
12	5916.0
13	5309.0
14	5004.0
15	4951.0
16	5004.0
17	5227.0
18	6085.0
19	7692.0
20	10271.0
21	13109.0
22	16165.0
23	18948.0
24	21802.0
25	24564.0
26	27877.0
27	30806.0
28	34646.0
29	41116.0
30	51611.0
31	65624.0
32	84450.0
33	111340.0
34	151674.0
35	213059.0
36	312938.0
37	543578.0
38	1564816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03109738617272	17.944724894523798	13.021037792330018	25.003139926973468
2	16.505853097271046	21.13597717365603	37.8247148121205	24.53345491695243
3	18.28199769604087	24.932961464261343	29.39332564592736	27.391715193770427
4	12.649376583997105	16.996939491310645	37.73544985517741	32.61823406951485
5	15.143048469874905	35.70620059178813	33.34914792185273	15.801603016484233
6	31.697805167208514	37.0952490089723	16.74334575477971	14.463600069039472
7	28.232587946203108	31.589616922791812	21.707699032793357	18.470096098211723
8	26.30797348490207	32.659997340568495	20.391502267589733	20.6405269069397
9	27.112193537728267	15.11161982270282	19.460990773032904	38.31519586653601
10	16.059665551763775	26.930576996902545	31.6282193492939	25.38153810203978
11	34.73028446946657	23.025108568033612	23.18699163990777	19.057615322592053
12	24.36469796736168	24.871541988412893	28.830244173486356	21.933515870739072
13	28.79242452918529	21.485909860791786	25.036515740596933	24.68514986942599
14	23.20452116509732	21.195422484411676	26.422468004406202	29.1775883460848
15	24.293312210026027	27.841688355776846	23.519237297725475	24.345762136471652
16	24.23388761242393	27.341539866589372	24.40036089960082	24.024211621385877
17	23.867090598801312	26.181430731345174	25.477687544136018	24.473791125717494
18	24.88662461899365	25.138752228607768	26.602546511489745	23.372076640908837
19	26.059420270584905	25.42970764575065	25.85055337139126	22.660318712273188
20	25.215198525856504	25.33102731992145	25.7983034222118	23.655470732010254
21	25.226400713844775	25.422402481481964	25.831663678167814	23.519533126505447
22	24.3445558313666	25.389135936871398	26.218502325281374	24.04780590648063
23	23.646024869973175	25.052462000592453	26.409811051919345	24.891702077515028
24	23.75871344469981	26.039395790497622	26.30051972972583	23.901371035076735
25	24.003116428125253	25.4922745625232	26.261593248418023	24.243015760933524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14643.0
1	14643.0
2	13689.5
3	12736.0
4	12736.0
5	12736.0
6	10587.5
7	8439.0
8	8439.0
9	8439.0
10	10282.0
11	12125.0
12	12125.0
13	12125.0
14	15465.0
15	18805.0
16	18805.0
17	18805.0
18	31134.5
19	43464.0
20	43464.0
21	43464.0
22	62862.5
23	82261.0
24	82261.0
25	82261.0
26	111412.5
27	140564.0
28	140564.0
29	140564.0
30	167109.0
31	193654.0
32	193654.0
33	193654.0
34	225412.5
35	257171.0
36	257171.0
37	257171.0
38	284488.5
39	311806.0
40	311806.0
41	311806.0
42	342334.0
43	372862.0
44	372862.0
45	372862.0
46	396958.0
47	421054.0
48	421054.0
49	421054.0
50	428884.0
51	436714.0
52	436714.0
53	436714.0
54	407001.5
55	377289.0
56	377289.0
57	377289.0
58	340053.0
59	302817.0
60	302817.0
61	302817.0
62	262321.0
63	221825.0
64	221825.0
65	221825.0
66	183814.0
67	145803.0
68	145803.0
69	145803.0
70	113793.5
71	81784.0
72	81784.0
73	81784.0
74	64915.5
75	48047.0
76	48047.0
77	48047.0
78	39162.5
79	30278.0
80	30278.0
81	30278.0
82	22616.0
83	14954.0
84	14954.0
85	14954.0
86	11201.5
87	7449.0
88	7449.0
89	7449.0
90	5057.0
91	2665.0
92	2665.0
93	2665.0
94	1449.5
95	234.0
96	234.0
97	234.0
98	143.5
99	53.0
100	53.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23761790995129647
2	0.672342376561176
3	0.6698420225784774
4	0.6780735250159011
5	0.6963064433841195
6	0.7103814697361649
7	0.7049031660662071
8	0.6997058010459908
9	0.6922328329628689
10	0.6927666163973776
11	0.6980201691475422
12	0.6989191728267148
13	0.6944522482958262
14	0.6876816268370578
15	0.6938060894014209
16	0.7022061550286894
17	0.7014476206743876
18	0.6961097863293007
19	0.6941713096460848
20	0.7014757145393617
21	0.6976830427678525
22	0.6986944219069217
23	0.7032737218977069
24	0.7068135488844488
25	0.6933565875618346
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3559496.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.64474561331685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.39497775887455	50.37861260791807
2	7.362663199037114	8.488376942900482
3	1.8185663874110642	3.1449239034971828
4	0.8464311482567324	1.9516923288178805
5	0.508808255584468	1.4665061229561078
6	0.3358460240790275	1.1615855173967655
7	0.24896668045839396	1.0046134672851244
8	0.19441208185359657	0.8965468002084725
9	0.14906589854668242	0.7733579221209606
>10	0.9756287198462215	10.626090456788372
>50	0.08523622176986739	3.3859163970284567
>100	0.06547116030218696	7.748413426581726
>500	0.00897042697925563	3.4643850601208994
>1k	0.00470823515083172	4.272857751677166
>5k	1.9824148003501978E-4	0.8215001347223371
>10k+	4.9560370008754945E-5	0.4146211599800132
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14513	0.40772626236972875	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8536	0.2398092314192796	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	8268	0.23228007560620942	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	6243	0.17538999903357105	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5708	0.16035978127240486	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4722	0.13265923040790045	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4188	0.1176571065117084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.8093864974142408E-5	0.0
2	0.0	0.0	0.0	2.8093864974142408E-5	0.0
3	0.0	0.0	0.0	2.8093864974142408E-5	0.0
4	0.0	0.0	0.0	8.428159492242721E-5	0.0
5	0.0	0.0	0.0	8.428159492242721E-5	0.0
6	0.0	0.0	0.0	8.428159492242721E-5	0.0
7	2.8093864974142408E-5	0.0	2.8093864974142408E-5	8.428159492242721E-5	0.0
8	2.8093864974142408E-5	0.0	2.8093864974142408E-5	8.428159492242721E-5	0.0
9	2.8093864974142408E-5	0.0	2.8093864974142408E-5	1.4046932487071204E-4	0.0
10	2.8093864974142408E-5	0.0	2.8093864974142408E-5	1.4046932487071204E-4	2.8093864974142408E-5
11	2.8093864974142408E-5	0.0	2.8093864974142408E-5	1.4046932487071204E-4	2.8093864974142408E-5
12	5.6187729948284816E-5	0.0	2.8093864974142408E-5	1.4046932487071204E-4	8.428159492242721E-5
13	8.428159492242721E-5	0.0	2.8093864974142408E-5	1.4046932487071204E-4	1.1237545989656963E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2810	0.0	14.09984	1
GTATCAA	8585	0.0	12.849208	1
CGAACGA	245	0.0	12.79608	16
AACCGGT	85	3.948644E-6	12.293752	14
CGCAGAT	240	0.0	12.269947	8
ATTACGG	135	3.7289283E-10	11.962268	13
AACCGCG	285	0.0	11.665824	7
CTCGACG	125	1.8360879E-8	11.399338	13
CGACCGG	60	0.0058807116	11.082689	10
CCGTCGT	365	0.0	10.670764	9
CGCGCTA	295	0.0	10.627254	16
CGACCGT	90	9.520805E-5	10.55569	19
GATTACG	135	5.696529E-8	10.55539	12
GCGTTAT	290	0.0	10.484226	1
CGTCGTA	355	0.0	10.436013	10
ACGCTTA	130	3.8452163E-7	10.230898	17
CGAGCCG	705	0.0	10.106225	15
CCGTCCA	160	6.186383E-9	10.093307	9
CGCTTAC	135	6.447426E-7	9.852116	18
GTATTAG	550	0.0	9.846833	1
>>END_MODULE