FastQCFastQC Report
Thu 2 Feb 2017
SRR4050239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559496
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT232360.6527890465391729No Hit
TATCAACGCAGAGTACTTTTTTTTT158990.44666435922389014No Hit
GGTATCAACGCAGAGTACTTTTTTT93430.2624809804534125No Hit
GTACTTTTTTTTTTTTTTTTTTTTT83810.2354546823482875No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83610.23489280504880466No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57960.16283204139012938No Hit
GCAGAGTACTTTTTTTTTTTTTTTT41650.11701094761730313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.016.0036131
GTATCAA91350.013.3675351
TATAACG701.0907738E-412.2153522
TCGTTAA3550.011.772531512
CCGTCGT4300.011.4864699
CGTCGTA4250.011.1746210
TACGCAG909.502809E-510.5576645
TAGATCG909.502809E-510.5576645
GTCGTAG4350.010.48087411
GTCCTAT6550.010.4785681
CGACCAT4550.010.43783110
CCGACCA4750.010.3982779
CGAACGA2750.010.35929916
ATACCGT5000.010.0669166
GATAACG2950.09.981256511
CGCGCTA2201.8189894E-129.92766116
CTCGACG1151.0295282E-59.91126213
TACCGTC4900.09.8852837
CGATAAC3000.09.8150410
TAACGAA3000.09.81490213
Produced by FastQC (version 0.11.5)