##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3559496
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.708732078923532	31.0	31.0	34.0	27.0	34.0
2	30.661829371349203	31.0	31.0	34.0	26.0	34.0
3	30.898742125289647	31.0	31.0	34.0	27.0	34.0
4	34.50016069690765	37.0	35.0	37.0	31.0	37.0
5	34.399721758361295	37.0	35.0	37.0	31.0	37.0
6	34.34292579623632	37.0	35.0	37.0	30.0	37.0
7	34.27414723882257	37.0	35.0	37.0	30.0	37.0
8	34.240956584864826	37.0	35.0	37.0	30.0	37.0
9	35.811990798697344	39.0	37.0	39.0	30.0	39.0
10	35.706912439289155	39.0	37.0	39.0	30.0	39.0
11	35.68951587528122	39.0	37.0	39.0	30.0	39.0
12	35.618256348651606	39.0	37.0	39.0	30.0	39.0
13	35.60895784122246	39.0	37.0	39.0	30.0	39.0
14	36.752376179099514	40.0	37.0	41.0	30.0	41.0
15	36.721094784205405	40.0	37.0	41.0	30.0	41.0
16	36.70648372690965	40.0	37.0	41.0	30.0	41.0
17	36.699044190525846	40.0	37.0	41.0	30.0	41.0
18	36.66475169518381	40.0	37.0	41.0	30.0	41.0
19	36.71802693415023	40.0	37.0	41.0	30.0	41.0
20	36.67396367350884	40.0	37.0	41.0	30.0	41.0
21	36.618868794908046	40.0	37.0	41.0	30.0	41.0
22	36.55694401679339	40.0	37.0	41.0	30.0	41.0
23	36.455121174458405	40.0	37.0	41.0	30.0	41.0
24	36.3928244335715	40.0	37.0	41.0	30.0	41.0
25	36.179907492521416	40.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8044576254087694
1101	2	0.9911139658928647
1101	3	0.91616500152643
1101	4	0.8094817145342432
1101	5	0.9056757917932643
1101	6	0.986629229828182
1101	7	0.9801283893336716
1101	8	0.9545251101055428
1101	9	1.1532466699033819
1101	10	1.17050653672014
1101	11	1.2051986799380359
1101	12	1.1798077689597974
1101	13	1.160138206230691
1101	14	1.3482902479991665
1101	15	1.3722452544786492
1101	16	1.41653527817423
1101	17	1.4203172552403274
1101	18	1.4267795023444592
1101	19	1.3046914326355576
1101	20	1.3560208558940872
1101	21	1.3928447963169006
1101	22	1.378850868420919
1101	23	1.318615949849928
1101	24	1.2636146890217432
1101	25	1.4240740532803642
1102	1	0.677879638832664
1102	2	0.881113864011148
1102	3	0.7935463815301915
1102	4	0.7510211902883128
1102	5	0.9414785697244881
1102	6	0.9816931540633291
1102	7	0.9302908906737883
1102	8	0.9420261861727681
1102	9	1.0094457191132804
1102	10	1.087939723110125
1102	11	1.0637346060021926
1102	12	1.0706243098025467
1102	13	1.0470914203698953
1102	14	1.2170636977356395
1102	15	1.3191979208867792
1102	16	1.235727615996062
1102	17	1.131519879189952
1102	18	1.272905300468544
1102	19	1.2436518521489575
1102	20	1.2528868340459738
1102	21	1.2564101741424523
1102	22	1.2329183464586322
1102	23	1.2655485670575999
1102	24	1.2815023613432146
1102	25	1.2997186304015997
1103	1	0.5751264367256432
1103	2	0.8036775133614675
1103	3	0.7187496948962888
1103	4	0.6428942060832625
1103	5	0.7295989531228102
1103	6	0.8120092120362585
1103	7	0.7588125848539136
1103	8	0.7544829166814822
1103	9	0.8477866841536823
1103	10	0.8598765812473204
1103	11	0.9635148015971993
1103	12	0.9368356794725798
1103	13	0.9439127578025932
1103	14	1.0410314851664708
1103	15	1.007942658398882
1103	16	1.1181909692673528
1103	17	1.0908289707473457
1103	18	1.0988310227742915
1103	19	1.1333156264105
1103	20	1.0663194581731474
1103	21	1.0714231803488374
1103	22	1.083568770948645
1103	23	1.1048476853897071
1103	24	1.160430998419777
1103	25	1.1788475487413876
1104	1	0.5361081376293804
1104	2	0.6692059905303829
1104	3	0.6337436383988866
1104	4	0.5384122318478859
1104	5	0.5436811819138541
1104	6	0.5913140191837556
1104	7	0.60407198405602
1104	8	0.6226966071903135
1104	9	0.7325859557124019
1104	10	0.6905109931190552
1104	11	0.7052087598879524
1104	12	0.709066680431313
1104	13	0.6937023173647958
1104	14	0.7566369770554218
1104	15	0.7336793660902146
1104	16	0.7686849601252845
1104	17	0.6649719639032483
1104	18	0.8238770072915926
1104	19	0.7234372216038523
1104	20	0.8683451862405676
1104	21	0.7410118370027661
1104	22	0.7970053887479338
1104	23	0.8644927198095544
1104	24	0.902131769231616
1104	25	0.8804721688602584
1105	1	0.48036044096035013
1105	2	0.6470514901760254
1105	3	0.4950996506833185
1105	4	0.5366073653229648
1105	5	0.648731973314753
1105	6	0.7332440924178698
1105	7	0.5735026478878211
1105	8	0.6120162386573611
1105	9	0.7479745689890436
1105	10	0.7414677613685257
1105	11	0.6789555116006838
1105	12	0.7512259055671535
1105	13	0.7464285162780939
1105	14	0.7761621365167954
1105	15	0.8462543719961957
1105	16	0.8936010952540343
1105	17	0.9412771847867845
1105	18	0.8937919612249701
1105	19	0.8560476633709158
1105	20	0.8362757319528313
1105	21	0.8119237198281795
1105	22	0.8732964352870312
1105	23	0.8177977044540157
1105	24	0.865800562049948
1105	25	0.9006446233842311
1106	1	0.4379058044872899
1106	2	0.6162704387996527
1106	3	0.4794789606402361
1106	4	0.4042756856631513
1106	5	0.5426006693875607
1106	6	0.5539168113046173
1106	7	0.5777763252302393
1106	8	0.48396071122549955
1106	9	0.6104402299522178
1106	10	0.5561449575481987
1106	11	0.5307585782088537
1106	12	0.5687339208214439
1106	13	0.618009068177571
1106	14	0.6167440721022714
1106	15	0.6994663234434384
1106	16	0.7365086357802824
1106	17	0.6071980109485722
1106	18	0.5905054257272369
1106	19	0.6998285010071754
1106	20	0.7021279362883774
1106	21	0.7140181479608785
1106	22	0.7755672552616133
1106	23	0.829046171712406
1106	24	0.7670198931558829
1106	25	0.7364580170147761
1107	1	0.15606583789782036
1107	2	0.4140363246199321
1107	3	0.347100592095142
1107	4	0.24457612221807068
1107	5	0.30388870367381315
1107	6	0.3183846422612078
1107	7	0.2818694454695674
1107	8	0.30277069197656914
1107	9	0.24280357035702593
1107	10	0.2913199302931062
1107	11	0.2877765403872985
1107	12	0.27178898751410685
1107	13	0.3255322628029589
1107	14	0.4443269718432745
1107	15	0.41053397130778535
1107	16	0.4024081499588874
1107	17	0.30045447911036405
1107	18	0.36954987129072236
1107	19	0.32742300720687467
1107	20	0.373541666083419
1107	21	0.34039451399731036
1107	22	0.3412753417783634
1107	23	0.36996456969973934
1107	24	0.31485091287294864
1107	25	0.3085004108605176
1108	1	0.21518839367821485
1108	2	0.3733445377838258
1108	3	0.29026690247178877
1108	4	0.27451187773935715
1108	5	0.3129239863126898
1108	6	0.38906428287769756
1108	7	0.39309080802868124
1108	8	0.39093066772520757
1108	9	0.3823342095417388
1108	10	0.45658430260082383
1108	11	0.4416769542569625
1108	12	0.47471280205964206
1108	13	0.40204310889374995
1108	14	0.521807899841292
1108	15	0.48885843241941274
1108	16	0.46769948876365675
1108	17	0.5305148802299939
1108	18	0.512286641447858
1108	19	0.47567959133830584
1108	20	0.548949076523364
1108	21	0.46654668308539726
1108	22	0.48295642047110476
1108	23	0.6125943433139724
1108	24	0.4892031795305982
1108	25	0.5562704889887371
1109	1	0.015282037841178209
1109	2	0.15641013853450403
1109	3	0.18915273877734862
1109	4	0.14378993864047374
1109	5	0.2074632554529643
1109	6	0.2033373285595843
1109	7	0.2248439769266426
1109	8	0.20701341331972856
1109	9	0.24069664052658624
1109	10	0.2560655745571978
1109	11	0.23623965580591744
1109	12	0.28560800422793875
1109	13	0.23292071834624295
1109	14	0.20737364129161762
1109	15	0.3786238413977898
1109	16	0.30311301817881997
1109	17	0.12363789564173544
1109	18	0.20639367337957282
1109	19	0.18837955578206333
1109	20	0.21579203415094383
1109	21	0.27363093008980854
1109	22	0.26327015964709233
1109	23	0.29387147401947544
1109	24	0.294738941436286
1109	25	0.2947419280121366
1110	1	-0.28077417094119994
1110	2	-0.15801998487311053
1110	3	-0.18261375147535475
1110	4	-0.24798783300268923
1110	5	-0.14451113576603092
1110	6	-0.3633182629382361
1110	7	-0.2957634163894127
1110	8	-0.22333047067296974
1110	9	-0.24602997911826208
1110	10	-0.3068266983928041
1110	11	-0.2770014356667616
1110	12	-0.2960587133779313
1110	13	-0.31863014625636765
1110	14	-0.2890854267575733
1110	15	-0.2685432774775549
1110	16	-0.22465720772504483
1110	17	-0.32983696734002166
1110	18	-0.38664815871626246
1110	19	-0.29289406316574684
1110	20	-0.33373023827652304
1110	21	-0.41538652414624977
1110	22	-0.31670336346927996
1110	23	-0.3101127152732346
1110	24	-0.3550275557178537
1110	25	-0.2505694797732829
1111	1	-0.3004443903502576
1111	2	-0.12604569736631532
1111	3	-0.25054752427748284
1111	4	-0.16080199921258043
1111	5	-0.1814759124061709
1111	6	-0.34302015859349666
1111	7	-0.24036994952231794
1111	8	-0.14075344177157234
1111	9	-0.24372045452875568
1111	10	-0.269476241109075
1111	11	-0.2809475137753097
1111	12	-0.1653228817661443
1111	13	-0.18686397544712463
1111	14	-0.16654637828102636
1111	15	-0.17716916128681248
1111	16	-0.1581229625823326
1111	17	-0.3155178135679364
1111	18	-0.24831263974670748
1111	19	-0.32169830569785773
1111	20	-0.16819168186363243
1111	21	-0.24714989184748504
1111	22	-0.238002560125544
1111	23	-0.276083603251692
1111	24	-0.25621732309029
1111	25	-0.18709979718754965
1112	1	-0.498874099009484
1112	2	-0.33245642837600897
1112	3	-0.36888224976916817
1112	4	-0.3294804928972468
1112	5	-0.27691659380440115
1112	6	-1.28643350939209
1112	7	-0.5475286492464164
1112	8	-0.4789754657167009
1112	9	-0.47557746721005145
1112	10	-0.5189866126887921
1112	11	-0.5052658307824203
1112	12	-0.46615343078277505
1112	13	-0.43995345958917653
1112	14	-0.5707552842976824
1112	15	-0.5221072418801285
1112	16	-0.5321549379284747
1112	17	-0.5918958921362929
1112	18	-0.5315002710525576
1112	19	-0.5749138750906226
1112	20	-0.580343800475525
1112	21	-0.6293677583015338
1112	22	-0.68578538975067
1112	23	-0.6293403045957575
1112	24	-0.7405022282969611
1112	25	-0.6416856933578501
1113	1	-0.665853156632604
1113	2	-0.7610119247723084
1113	3	-0.6547857018622025
1113	4	-0.6243418346291918
1113	5	-0.6969550372942805
1113	6	-1.1506176681474543
1113	7	-0.738140601622213
1113	8	-0.7837022401935911
1113	9	-0.8280624568020372
1113	10	-0.7892901447972278
1113	11	-0.8293521962783359
1113	12	-0.8666228444443504
1113	13	-0.8076796535031932
1113	14	-0.8559221284421241
1113	15	-0.8248869498539904
1113	16	-0.9109566471296375
1113	17	-0.9222141338429637
1113	18	-0.918013690176835
1113	19	-0.9968836677725221
1113	20	-0.9243227167180521
1113	21	-0.9326686192191218
1113	22	-1.0120329231104748
1113	23	-0.88422500420711
1113	24	-0.9149658092569055
1113	25	-0.9451212418544017
1114	1	-0.8861101472749411
1114	2	-0.8939914802036171
1114	3	-0.9346166613734184
1114	4	-0.787992134051251
1114	5	-1.0626030287711856
1114	6	-1.4922502755420837
1114	7	-1.1648265135639946
1114	8	-1.2842436695612491
1114	9	-1.3108427026004463
1114	10	-1.5125372448893017
1114	11	-1.2986957191043587
1114	12	-1.24821714825012
1114	13	-1.4165814962375478
1114	14	-1.5940297700330603
1114	15	-1.5938144640488616
1114	16	-1.5370643198481773
1114	17	-1.5680365407034103
1114	18	-1.6318963992255888
1114	19	-1.4313574263245386
1114	20	-1.4958373260363231
1114	21	-1.5350120331964305
1114	22	-1.398192926415092
1114	23	-1.5776382571646792
1114	24	-1.6296687746208889
1114	25	-1.5520712714843512
1115	1	-1.1987493556584816
1115	2	-1.9111870901723158
1115	3	-1.5358139214494422
1115	4	-1.0829562962683
1115	5	-1.1779730327100069
1115	6	-1.6643518472615426
1115	7	-1.3591533567859315
1115	8	-1.4045120411834446
1115	9	-1.523360736082708
1115	10	-1.5613620483952317
1115	11	-1.6728913500489995
1115	12	-1.552114730501053
1115	13	-1.5615391326064127
1115	14	-1.7775257445946835
1115	15	-1.649496166580505
1115	16	-1.8013830212976174
1115	17	-1.671137464499374
1115	18	-1.87051957344476
1115	19	-1.8150137891430518
1115	20	-1.7813695011869797
1115	21	-1.9293889467654566
1115	22	-2.0962862134149063
1115	23	-1.9760665377046749
1115	24	-1.9532263485280623
1115	25	-2.0511339576943826
1116	1	-1.0773128471420463
1116	2	-1.1449623355683016
1116	3	-1.1621236595682483
1116	4	-1.0142557528137104
1116	5	-0.9946130174758565
1116	6	-1.1980708687627981
1116	7	-1.2150911130410478
1116	8	-1.285430311901031
1116	9	-1.3285089590238286
1116	10	-1.4021795072892402
1116	11	-1.3576410937477519
1116	12	-1.3041206706376443
1116	13	-1.34488329738911
1116	14	-1.5640590054477173
1116	15	-1.555882090198672
1116	16	-1.5165033555631524
1116	17	-1.594711717313885
1116	18	-1.638090601550438
1116	19	-1.6126166251744678
1116	20	-1.585341856818708
1116	21	-1.7635248591277772
1116	22	-1.6878447132056564
1116	23	-1.6408143586827748
1116	24	-1.6544494904086093
1116	25	-1.8813225725309906
1201	1	0.6743315782841606
1201	2	0.8397379757080827
1201	3	0.8066095364298427
1201	4	0.7565073666149402
1201	5	0.7936205837116859
1201	6	0.877110709128246
1201	7	0.8694846119451327
1201	8	0.8699541636626336
1201	9	0.9605056732383375
1201	10	1.0065250603698246
1201	11	1.0046938956870264
1201	12	1.028950741163591
1201	13	0.9860684434108222
1201	14	1.1733678143073476
1201	15	1.1742797205059006
1201	16	1.1536932559926996
1201	17	1.2051398394467157
1201	18	1.2042291648786687
1201	19	1.257338387435638
1201	20	1.1928869729790037
1201	21	1.220955816743384
1201	22	1.2346344024967593
1201	23	1.247902440396274
1201	24	1.2098546440385505
1201	25	1.3304674938897847
1202	1	0.6821775638491125
1202	2	0.8071561553740025
1202	3	0.7629769729424005
1202	4	0.6873044861859441
1202	5	0.6819156522964605
1202	6	0.7671181396691367
1202	7	0.7580966060048553
1202	8	0.7855946911317631
1202	9	0.8619858627015304
1202	10	0.8370389041605506
1202	11	0.9303303396205749
1202	12	0.9270857217277992
1202	13	0.9594940248749921
1202	14	1.1451267951309916
1202	15	1.1311570080398496
1202	16	1.1376472492300422
1202	17	1.133715124232907
1202	18	1.1087687913888047
1202	19	1.1242970535273216
1202	20	1.1288293095612332
1202	21	1.1648579748905163
1202	22	1.1688123756278728
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2211	3	-0.2526048386191029
2211	4	-0.2671708242370414
2211	5	-0.30508735066148773
2211	6	-0.21850326604294423
2211	7	-0.3478839630568302
2211	8	-0.24312700899022843
2211	9	-0.31345383347113653
2211	10	-0.38347112839650066
2211	11	-0.3641400832055979
2211	12	-0.2895594595172213
2211	13	-0.2627309222962637
2211	14	-0.41058351348583244
2211	15	-0.3960138033704652
2211	16	-0.4084606195123044
2211	17	-0.4934524845608479
2211	18	-0.5183433486630591
2211	19	-0.4488629711241714
2211	20	-0.39643861383910917
2211	21	-0.4405637952851009
2211	22	-0.49431592869341046
2211	23	-0.4741115632745263
2211	24	-0.47261924555137824
2211	25	-0.4401699169090989
2212	1	-0.3913809780021289
2212	2	-0.42770407735443783
2212	3	-0.4467467839481145
2212	4	-0.516008756538838
2212	5	-0.41544828540742174
2212	6	-0.3847703608687638
2212	7	-0.5646378626923365
2212	8	-0.5738263404812827
2212	9	-0.6287777717313148
2212	10	-0.6230427802929341
2212	11	-0.627693994594722
2212	12	-0.6464725384431986
2212	13	-0.6063320422230731
2212	14	-0.7964640250225798
2212	15	-0.6895536937460491
2212	16	-0.7559539192922742
2212	17	-0.7013914449094898
2212	18	-0.6863299583100613
2212	19	-0.7249638421637385
2212	20	-0.7296535155845589
2212	21	-0.7586376360735798
2212	22	-0.6732021549882674
2212	23	-0.8325815579985516
2212	24	-0.8827033014617598
2212	25	-0.8700165519316343
2213	1	-0.6786722513292389
2213	2	-0.9499646912365307
2213	3	-0.7048492352560771
2213	4	-0.7161310492939634
2213	5	-0.7752015501666492
2213	6	-0.6358233348410849
2213	7	-0.7850591925948933
2213	8	-0.810323712205367
2213	9	-0.8976914669884835
2213	10	-1.0199425348671127
2213	11	-0.9066936746220478
2213	12	-0.9710737564964234
2213	13	-0.9005718508394693
2213	14	-1.058947010358473
2213	15	-1.1130598712238964
2213	16	-1.113477872674956
2213	17	-1.1675242234385337
2213	18	-1.0821858422824135
2213	19	-1.1281850138574399
2213	20	-1.1871614297577509
2213	21	-1.0246498495899843
2213	22	-1.110321084692636
2213	23	-1.11671690023568
2213	24	-1.1277862706668174
2213	25	-1.128981199100302
2214	1	-0.814277302834995
2214	2	-0.8770004704550303
2214	3	-0.8800916307690763
2214	4	-0.8576995248553345
2214	5	-0.9924235622150803
2214	6	-0.8719147596175532
2214	7	-0.9209598299280657
2214	8	-0.927075185938115
2214	9	-1.0586822278550656
2214	10	-1.0930291075682064
2214	11	-1.0986698152222445
2214	12	-1.1082043420673102
2214	13	-1.1247966903904754
2214	14	-1.3907140339920545
2214	15	-1.4403412748603301
2214	16	-1.349236938505662
2214	17	-1.2465243888675346
2214	18	-1.3238987761611227
2214	19	-1.3333499709368297
2214	20	-1.2686269951220552
2214	21	-1.3404040563659265
2214	22	-1.3821534539073497
2214	23	-1.4988584180543896
2214	24	-1.5453354483961235
2214	25	-1.5059312531755538
2215	1	-1.5657363242116453
2215	2	-3.291034336864776
2215	3	-1.8973210323283958
2215	4	-1.5167943313303383
2215	5	-1.5548667339970166
2215	6	-1.4803160828408295
2215	7	-1.4934871908368947
2215	8	-1.5948350089732273
2215	9	-1.8491877317474206
2215	10	-1.9194998903514389
2215	11	-1.8494211702923522
2215	12	-1.7796997959337304
2215	13	-1.8357855230618867
2215	14	-2.040022879486436
2215	15	-2.1300735854942516
2215	16	-2.245134183326499
2215	17	-2.23283797633313
2215	18	-2.193366610603128
2215	19	-2.13966813166342
2215	20	-2.096125374289983
2215	21	-2.1321718645228316
2215	22	-2.222616681148132
2215	23	-2.3710227175341174
2215	24	-2.4462894910869935
2215	25	-2.390579390785618
2216	1	-1.294054970199575
2216	2	-2.033740175749493
2216	3	-1.4941918106850203
2216	4	-1.2341619475427947
2216	5	-1.4648634896663282
2216	6	-1.4603918669552982
2216	7	-1.5268957939201115
2216	8	-1.5344067381535638
2216	9	-1.7924071524930199
2216	10	-1.692526084128552
2216	11	-1.702699741368356
2216	12	-1.833462189205079
2216	13	-1.8853591355833785
2216	14	-1.938100242157084
2216	15	-1.9035666849418504
2216	16	-1.8993549042004787
2216	17	-1.8556283690888336
2216	18	-2.173447762000272
2216	19	-2.0166093268518424
2216	20	-2.184403516462595
2216	21	-1.9962335461020757
2216	22	-2.0592089454561275
2216	23	-2.2002591209371616
2216	24	-2.181479361107705
2216	25	-2.277895016239384
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58649.0
3	6.0
4	980.0
5	8651.0
6	10443.0
7	8709.0
8	6876.0
9	5696.0
10	5328.0
11	4941.0
12	4408.0
13	4150.0
14	4082.0
15	4119.0
16	4146.0
17	4488.0
18	5088.0
19	6732.0
20	9066.0
21	11789.0
22	14302.0
23	16882.0
24	19589.0
25	22414.0
26	24922.0
27	28460.0
28	33193.0
29	40132.0
30	51608.0
31	67091.0
32	87152.0
33	115517.0
34	156016.0
35	214458.0
36	313664.0
37	539194.0
38	1646555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14217956036676	18.611412986024483	13.586203494231489	25.66020395937727
2	16.10239019086125	21.872592459393168	36.89092559136643	25.134091758379157
3	17.799682876452174	25.309032514715568	29.68779849731535	27.203486111516913
4	13.040638337562397	17.628113643055084	36.73343080031555	32.59781721906697
5	14.943260521510743	36.18694739019761	33.173649900608936	15.696142187682705
6	31.535030728709394	37.588790166813	16.56622300263389	14.309956101843724
7	27.85911131501856	31.889314590954694	21.65803353366023	18.593540560366517
8	26.27824256203931	32.85816989406028	20.27011271599732	20.593474827903087
9	26.94135053746039	15.043215423025636	19.503976515714726	38.511457523799244
10	15.915472418278176	27.318075389449593	31.50056491650728	25.265887275764953
11	34.730101843431	22.9767647284414	23.192116848121547	19.101016580006053
12	24.240666087505748	25.08726748805621	28.939483677664356	21.732582746773687
13	28.89614403745927	21.36863235672627	25.205824799682595	24.529398806131866
14	23.365817464226204	21.06277527624903	26.389322407082812	29.18208485244196
15	24.111704510138583	27.84359929886124	23.90160453773229	24.143091653267888
16	24.036084119480954	27.218577134623988	24.673543632035713	24.071795113859352
17	23.743296066548087	25.91209878596608	25.93229943828701	24.412305709198826
18	24.83628970338158	24.865057877689747	27.07132962062813	23.22732279830054
19	25.800588815539022	25.063206783038133	26.661251818801695	22.474952582621146
20	24.99263208791036	24.918320829656402	26.586545363722003	23.502501718711237
21	25.006187807616918	24.99284293307419	26.629570724885156	23.371398534423736
22	24.215808262581216	24.96557796292899	26.948556999550217	23.870056774939577
23	23.478271130241733	24.628243780042027	27.198684942837097	24.694800146879142
24	23.619182902181223	25.5586509777832	27.06928008208176	23.75288603795382
25	23.82613303156056	24.997534704477793	27.11544129070793	24.06089097325372
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2249.0
1	2249.0
2	3098.0
3	3947.0
4	3947.0
5	3947.0
6	7832.0
7	11717.0
8	11717.0
9	11717.0
10	13006.5
11	14296.0
12	14296.0
13	14296.0
14	16521.0
15	18746.0
16	18746.0
17	18746.0
18	32235.5
19	45725.0
20	45725.0
21	45725.0
22	65972.5
23	86220.0
24	86220.0
25	86220.0
26	118008.0
27	149796.0
28	149796.0
29	149796.0
30	175554.0
31	201312.0
32	201312.0
33	201312.0
34	231183.5
35	261055.0
36	261055.0
37	261055.0
38	285517.5
39	309980.0
40	309980.0
41	309980.0
42	340948.5
43	371917.0
44	371917.0
45	371917.0
46	395063.5
47	418210.0
48	418210.0
49	418210.0
50	426280.5
51	434351.0
52	434351.0
53	434351.0
54	404778.0
55	375205.0
56	375205.0
57	375205.0
58	338712.0
59	302219.0
60	302219.0
61	302219.0
62	261898.5
63	221578.0
64	221578.0
65	221578.0
66	183377.0
67	145176.0
68	145176.0
69	145176.0
70	113172.0
71	81168.0
72	81168.0
73	81168.0
74	64689.0
75	48210.0
76	48210.0
77	48210.0
78	39535.5
79	30861.0
80	30861.0
81	30861.0
82	23080.0
83	15299.0
84	15299.0
85	15299.0
86	11295.0
87	7291.0
88	7291.0
89	7291.0
90	4998.5
91	2706.0
92	2706.0
93	2706.0
94	1465.0
95	224.0
96	224.0
97	224.0
98	131.0
99	38.0
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34333512384899434
2	0.010282354580536121
3	0.0
4	0.0
5	0.05146796063262889
6	0.003399357661871231
7	0.004129798151198934
8	5.337834345087057E-4
9	0.009832852740949843
10	0.015816845980442175
11	0.005871617779595763
12	0.00910241225162214
13	0.01809244904334771
14	0.0032869822019746617
15	0.020059019591537677
16	0.010338542310484406
17	0.005759242319699194
18	1.9665705481899685E-4
19	0.004129798151198934
20	0.004017422691302364
21	0.0021632276030089654
22	8.428159492242723E-5
23	0.0030060435522332373
24	0.003315076066948804
25	0.002387978522802105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3559496.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.25648331436577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.35692509627367	50.01750324169093
2	7.3901717375401415	8.462704895615293
3	1.8165862375247501	3.12034018793827
4	0.8512976371974124	1.9496923583901056
5	0.5127930733331484	1.4680364023510875
6	0.33512176258454746	1.1512736164621793
7	0.25150803391613896	1.0080325883143846
8	0.19489674876473526	0.8927281954937761
9	0.14912145735973023	0.768435321161817
>10	0.9788693862400354	10.660613254920841
>50	0.08308424004305938	3.2830209355490805
>100	0.06524940420630274	7.630233272890343
>500	0.009402946366278267	3.618925938697305
>1k	0.004676858136214568	3.966702272016762
>5k	1.969203425774555E-4	0.8986430022466808
>10k+	9.846017128872775E-5	1.1031145162612168
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	23236	0.6527890465391729	No Hit
TATCAACGCAGAGTACTTTTTTTTT	15899	0.44666435922389014	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9343	0.2624809804534125	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8381	0.2354546823482875	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	8361	0.23489280504880466	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	5796	0.16283204139012938	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4165	0.11701094761730313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.8093864974142408E-5	2.8093864974142408E-5
2	0.0	0.0	0.0	2.8093864974142408E-5	2.8093864974142408E-5
3	0.0	0.0	0.0	2.8093864974142408E-5	2.8093864974142408E-5
4	0.0	0.0	0.0	1.1237545989656963E-4	2.8093864974142408E-5
5	0.0	0.0	0.0	1.1237545989656963E-4	2.8093864974142408E-5
6	0.0	0.0	0.0	1.1237545989656963E-4	5.6187729948284816E-5
7	0.0	0.0	0.0	1.1237545989656963E-4	5.6187729948284816E-5
8	0.0	0.0	0.0	1.1237545989656963E-4	5.6187729948284816E-5
9	0.0	0.0	0.0	1.4046932487071204E-4	5.6187729948284816E-5
10	0.0	0.0	0.0	1.6856318984485443E-4	5.6187729948284816E-5
11	0.0	0.0	0.0	1.6856318984485443E-4	5.6187729948284816E-5
12	0.0	0.0	0.0	1.9665705481899685E-4	1.1237545989656963E-4
13	0.0	0.0	0.0	1.9665705481899685E-4	1.1237545989656963E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2740	0.0	16.003613	1
GTATCAA	9135	0.0	13.367535	1
TATAACG	70	1.0907738E-4	12.215352	2
TCGTTAA	355	0.0	11.7725315	12
CCGTCGT	430	0.0	11.486469	9
CGTCGTA	425	0.0	11.17462	10
TACGCAG	90	9.502809E-5	10.557664	5
TAGATCG	90	9.502809E-5	10.557664	5
GTCGTAG	435	0.0	10.480874	11
GTCCTAT	655	0.0	10.478568	1
CGACCAT	455	0.0	10.437831	10
CCGACCA	475	0.0	10.398277	9
CGAACGA	275	0.0	10.359299	16
ATACCGT	500	0.0	10.066916	6
GATAACG	295	0.0	9.9812565	11
CGCGCTA	220	1.8189894E-12	9.927661	16
CTCGACG	115	1.0295282E-5	9.911262	13
TACCGTC	490	0.0	9.885283	7
CGATAAC	300	0.0	9.81504	10
TAACGAA	300	0.0	9.814902	13
>>END_MODULE