FastQCFastQC Report
Thu 2 Feb 2017
SRR4050238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510766
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136320.38829133015416006No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN124270.35396833625482305No Hit
TATCAACGCAGAGTACTTTTTTTTT79900.22758566079311465No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN54880.15631916225689776No Hit
GGTATCAACGCAGAGTACTTTTTTT54190.15435377920374072No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43090.12273674747904019No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37920.10801061648654453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.014.4270871
GTATCAA80800.013.0475221
CCGTCGT4350.012.4474869
CGTCGTA4500.012.24366710
GTCGTAG4350.012.22928411
ACCGTCG4650.012.0528268
ATACCGT4900.012.0198176
TACCGTC4750.011.799427
AGTCGTA855.328915E-511.17608317
CCGACCA4450.011.1004199
CGACCAT4400.011.01066410
CGAACGA2250.010.97739616
ATCGCCA3550.010.9714516
CGTTATT2450.010.8575452
TAGATCG1201.5256683E-610.2914575
GTATTAG5100.010.2518051
ACACCGT2053.6379788E-1210.1946216
GCGTTAT2450.010.0882321
CATCGCC4000.09.97465415
CGGTTCT5450.09.76112812
Produced by FastQC (version 0.11.5)