##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050238_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3510766
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.100561245038833	31.0	31.0	34.0	26.0	34.0
2	30.131575274455773	31.0	31.0	34.0	26.0	34.0
3	30.279963688836	31.0	31.0	34.0	26.0	34.0
4	33.69213328373352	37.0	35.0	37.0	28.0	37.0
5	33.752231564279704	37.0	35.0	37.0	28.0	37.0
6	33.79959473231768	37.0	35.0	37.0	30.0	37.0
7	33.685942327116074	37.0	35.0	37.0	29.0	37.0
8	33.64488205707814	37.0	35.0	37.0	29.0	37.0
9	35.12263648445952	39.0	35.0	39.0	28.0	39.0
10	35.0157344579502	39.0	35.0	39.0	28.0	39.0
11	35.05123326362395	39.0	35.0	39.0	28.0	39.0
12	34.97600637581656	39.0	35.0	39.0	27.0	39.0
13	34.96190717353421	39.0	35.0	39.0	27.0	39.0
14	36.0421187285054	40.0	37.0	41.0	27.0	41.0
15	36.034452310407474	40.0	36.0	41.0	27.0	41.0
16	36.04257361498887	40.0	37.0	41.0	27.0	41.0
17	36.02958414203624	40.0	37.0	41.0	27.0	41.0
18	35.973376465420934	40.0	36.0	41.0	27.0	41.0
19	36.01613636454267	40.0	37.0	41.0	27.0	41.0
20	35.99467893901217	40.0	37.0	41.0	27.0	41.0
21	35.915060132176286	40.0	37.0	41.0	27.0	41.0
22	35.832324341753335	40.0	36.0	41.0	27.0	41.0
23	35.76791389685328	40.0	36.0	41.0	27.0	41.0
24	35.72184360905854	40.0	36.0	41.0	27.0	41.0
25	35.40176844597447	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.2970083148308227
1101	2	1.388051901253565
1101	3	1.3941458247946308
1101	4	1.4137427274013206
1101	5	1.401242320236527
1101	6	1.3650318491407774
1101	7	1.3233785534114233
1101	8	1.3912180627592008
1101	9	1.5340984345469622
1101	10	1.6230139675118949
1101	11	1.5299785938412782
1101	12	1.512040953707043
1101	13	1.6196997359936205
1101	14	1.772573051059183
1101	15	1.738881544435273
1101	16	1.8080841743915315
1101	17	1.8078297776108343
1101	18	1.869285769561742
1101	19	1.7934608906044858
1101	20	1.8141969572200054
1101	21	1.7461484514612167
1101	22	1.81005793831757
1101	23	1.7520219424371675
1101	24	1.8625837906792881
1101	25	1.7883788531149847
1102	1	1.0556872226956457
1102	2	1.1001313149611498
1102	3	1.115004544517415
1102	4	1.1504520612329543
1102	5	1.1318884253619927
1102	6	1.1025591205567267
1102	7	1.0166486809867337
1102	8	0.9755533286544846
1102	9	1.2629250793145346
1102	10	1.2058133466005572
1102	11	1.235683339682332
1102	12	1.1535121923005818
1102	13	1.1858895729142418
1102	14	1.4219810707814773
1102	15	1.4590452630844126
1102	16	1.4320508930193654
1102	17	1.3702462832306779
1102	18	1.4124340260761485
1102	19	1.3622012503331078
1102	20	1.4281435306516173
1102	21	1.4475471411219232
1102	22	1.4879265798363477
1102	23	1.478865211499297
1102	24	1.4909958935328902
1102	25	1.5045430372837103
1103	1	1.0395606400261173
1103	2	1.1229862982248946
1103	3	1.101121347838749
1103	4	1.2475141038116462
1103	5	1.2071856604785935
1103	6	1.1862491323302393
1103	7	1.1560238164320467
1103	8	1.175850991553979
1103	9	1.3471960433817927
1103	10	1.4129731095912632
1103	11	1.3604459664228443
1103	12	1.2697755822520236
1103	13	1.2751722101361267
1103	14	1.5077121387985883
1103	15	1.5294570788086759
1103	16	1.5211460457165842
1103	17	1.5823553324436546
1103	18	1.5232017238706987
1103	19	1.6002773686801461
1103	20	1.547528579560847
1103	21	1.5761783126301268
1103	22	1.6343676712645134
1103	23	1.5797624086565207
1103	24	1.5153385900857188
1103	25	1.5898306447416175
1104	1	0.9304733866292487
1104	2	1.0883789371596357
1104	3	0.9915818003734138
1104	4	1.13430625400553
1104	5	1.101167834940405
1104	6	1.0269707054715127
1104	7	1.0395233338019807
1104	8	1.0375616333617472
1104	9	1.1308997098273608
1104	10	1.0995834006956926
1104	11	1.162459500986138
1104	12	1.1581495194786413
1104	13	1.2104935481385297
1104	14	1.3239953616219964
1104	15	1.3294418497880045
1104	16	1.3404124828815327
1104	17	1.3736550286739444
1104	18	1.4342546105895408
1104	19	1.4106707437859214
1104	20	1.3812468081581244
1104	21	1.4081962432982422
1104	22	1.3957559843553895
1104	23	1.439357986190764
1104	24	1.4688079170336152
1104	25	1.4357603773254226
1105	1	0.6727574856369287
1105	2	0.8203156931542352
1105	3	0.8210272220701071
1105	4	0.9040363487019221
1105	5	0.8401825966958256
1105	6	0.8024363365216516
1105	7	0.7454263766766474
1105	8	0.8027033153632033
1105	9	0.9589008102859964
1105	10	0.9743783296544493
1105	11	0.8421334030522871
1105	12	0.8453423482247828
1105	13	0.9013745111668499
1105	14	0.9738001285417042
1105	15	0.9659062802074487
1105	16	0.9964108326032743
1105	17	0.9974081375412993
1105	18	0.9847470412490722
1105	19	1.0177016338566176
1105	20	1.0374688140123496
1105	21	1.0691488283210617
1105	22	1.1246233784746593
1105	23	1.1205454900445204
1105	24	1.0294382512211087
1105	25	1.0453153573412024
1106	1	0.6631026192937348
1106	2	0.7809201535226471
1106	3	0.7285681897061664
1106	4	0.8191164670131386
1106	5	0.7822932944590661
1106	6	0.737656809263953
1106	7	0.6415967256529811
1106	8	0.6458308540345143
1106	9	0.7640709918699784
1106	10	0.8098604061082995
1106	11	0.8646700583055988
1106	12	0.96835141331335
1106	13	0.9377520400768233
1106	14	1.0388758590856284
1106	15	1.1240287333211612
1106	16	1.0646220964464845
1106	17	1.07842020022877
1106	18	1.0427182227027743
1106	19	1.0610362674123266
1106	20	1.0673096907617037
1106	21	1.1565287590987197
1106	22	1.1810652973863967
1106	23	1.0635015385334086
1106	24	1.0612912382081845
1106	25	1.0342562894885319
1107	1	0.6018549513198188
1107	2	0.6734889330444354
1107	3	0.6902685705052001
1107	4	0.7761621018688842
1107	5	0.6412865710607392
1107	6	0.6279609865150633
1107	7	0.616233728955244
1107	8	0.62228038450003
1107	9	0.7044009707340848
1107	10	0.6701526246695764
1107	11	0.6804185857524132
1107	12	0.6192403139184464
1107	13	0.6336301443438188
1107	14	0.831248399525272
1107	15	0.7625185851934333
1107	16	0.8081070501277878
1107	17	0.7761221771017119
1107	18	0.7705760525894974
1107	19	0.8358102836197006
1107	20	0.8400099380107875
1107	21	0.8162598379961921
1107	22	0.8664027067739468
1107	23	0.8711084502364201
1107	24	0.8604609223547683
1107	25	0.8398767105735274
1108	1	0.5407708905544233
1108	2	0.6094407958053907
1108	3	0.5648051174217557
1108	4	0.6109816168180373
1108	5	0.5762698517193456
1108	6	0.5211905222855009
1108	7	0.4389161568203619
1108	8	0.4596644896157258
1108	9	0.5792378857226055
1108	10	0.6118000730530326
1108	11	0.5312838470495009
1108	12	0.4788973949585298
1108	13	0.5460124226060472
1108	14	0.5851218938778473
1108	15	0.6482645327804377
1108	16	0.6130172878576516
1108	17	0.6981017049351621
1108	18	0.6740020673918039
1108	19	0.7011448700830343
1108	20	0.6921898880814581
1108	21	0.767013141964263
1108	22	0.7428706064025761
1108	23	0.6397667046599125
1108	24	0.6348286454108845
1108	25	0.5702441163651955
1109	1	0.35024139753759087
1109	2	0.5509017531560474
1109	3	0.41230212463513993
1109	4	0.4826604763190687
1109	5	0.5230286041280721
1109	6	0.3205146470446323
1109	7	0.3955708523405619
1109	8	0.398338591353415
1109	9	0.4843399474200538
1109	10	0.5446339265002749
1109	11	0.44135066037123494
1109	12	0.5221448498109353
1109	13	0.44002691758396395
1109	14	0.49981843725193187
1109	15	0.4543511274838039
1109	16	0.47701012563327083
1109	17	0.43682225881384795
1109	18	0.49919007578183994
1109	19	0.5653802344185621
1109	20	0.5388481306080166
1109	21	0.5823494083990113
1109	22	0.5773885375429089
1109	23	0.49154757469964494
1109	24	0.4560096279998618
1109	25	0.5106673015086969
1110	1	0.10435070320500017
1110	2	0.3390748230946201
1110	3	0.19658396334532569
1110	4	0.235441438625827
1110	5	0.2767772421497696
1110	6	0.22704737400153618
1110	7	0.040028413886190606
1110	8	0.14898782469780514
1110	9	0.22656316974228474
1110	10	0.2566119137726872
1110	11	0.14641617744926094
1110	12	0.15520376622889387
1110	13	0.1394396799128259
1110	14	0.23410491853164217
1110	15	0.17556280654644496
1110	16	0.140285063653387
1110	17	0.20560865324276278
1110	18	0.21349913826703215
1110	19	0.15747172777842877
1110	20	0.20087472274091311
1110	21	0.1947248880522423
1110	22	0.20673144025423085
1110	23	0.2510868865255631
1110	24	0.23615272652023833
1110	25	0.25848526225213675
1111	1	-0.04492948708661615
1111	2	-0.028609524464563663
1111	3	-0.1667763981877073
1111	4	-0.05735634453879612
1111	5	-0.010905182858699902
1111	6	-0.08364117526594583
1111	7	-0.12688285207109118
1111	8	-0.11180530343976613
1111	9	-0.04592306963018444
1111	10	-0.04057059983966127
1111	11	-0.02942936868296897
1111	12	0.003649093254622926
1111	13	-0.005922772759333839
1111	14	-0.14164344821331554
1111	15	-0.06196938631694593
1111	16	-0.07115685961024809
1111	17	-0.05726199839236301
1111	18	-0.02164102751352459
1111	19	-0.08334627372425985
1111	20	-0.08265458366677336
1111	21	0.07873705271433096
1111	22	0.0520002051015922
1111	23	-0.12260931802915565
1111	24	-0.1352439846616562
1111	25	-0.2275362308714577
1112	1	-0.16435755042520128
1112	2	-0.06017340509530911
1112	3	-0.11449237148658753
1112	4	-0.06404759821509032
1112	5	-0.09542424736825694
1112	6	-0.15014938457592564
1112	7	-0.24956071174752736
1112	8	-0.18166176526936795
1112	9	-0.2885213560362203
1112	10	-0.2870130763824221
1112	11	-0.1975566474239443
1112	12	-0.21838194147557743
1112	13	-0.3227106377668676
1112	14	-0.37092304094949924
1112	15	-0.3516263458437834
1112	16	-0.25242777742477074
1112	17	-0.23444488136862418
1112	18	-0.2436893559991944
1112	19	-0.2027648516166778
1112	20	-0.2155117017792776
1112	21	-0.13751822511164136
1112	22	-0.23170159886530683
1112	23	-0.27980664087883156
1112	24	-0.2946868562938789
1112	25	-0.3302427998316091
1113	1	-0.5629617847841217
1113	2	-0.4989419793102492
1113	3	-0.6323932097819878
1113	4	-0.541248370017378
1113	5	-0.5177304342698292
1113	6	-0.5657696326889337
1113	7	-0.6017102479486098
1113	8	-0.5959702319644293
1113	9	-0.6433184853008882
1113	10	-0.7116377793219897
1113	11	-0.5993487023012207
1113	12	-0.6195062457306477
1113	13	-0.7464625442123491
1113	14	-0.7308463687444089
1113	15	-0.7756316948845239
1113	16	-0.7463019059022145
1113	17	-0.7518941376240278
1113	18	-0.7230351036913234
1113	19	-0.8860904240573149
1113	20	-0.9540522707788526
1113	21	-0.927454588205741
1113	22	-0.9151510472590587
1113	23	-1.0586835896100126
1113	24	-1.0991159816334246
1113	25	-1.1574770251252673
1114	1	-0.9221266503590542
1114	2	-0.9056925433619902
1114	3	-1.0032680660061075
1114	4	-0.9436889801170452
1114	5	-0.9784618638095353
1114	6	-1.0559658949437107
1114	7	-0.91241654327942
1114	8	-0.9968080921190889
1114	9	-1.2158380894477574
1114	10	-1.0773559899957093
1114	11	-1.0912593916218327
1114	12	-1.291240686662988
1114	13	-1.1298750833613695
1114	14	-1.2393087094315405
1114	15	-1.208656277589462
1114	16	-1.1200700697346164
1114	17	-1.5590346814729017
1114	18	-1.3664078444954626
1114	19	-1.3316328426257087
1114	20	-1.2031972451600623
1114	21	-1.3440060351176086
1114	22	-1.384502434569903
1114	23	-1.540545382603021
1114	24	-1.511086322843056
1114	25	-1.4753500295138338
1115	1	-1.2104562801521972
1115	2	-1.2472510326315813
1115	3	-1.3697981665327674
1115	4	-1.4240639550595517
1115	5	-1.209325354770833
1115	6	-1.3720277692270173
1115	7	-1.2535946632516897
1115	8	-1.2720216972825966
1115	9	-1.9737300454062492
1115	10	-1.6401469942654785
1115	11	-1.656015381292498
1115	12	-1.6783896894545336
1115	13	-1.7220311190722484
1115	14	-1.8721247920392727
1115	15	-1.8651887990912428
1115	16	-2.033077956175063
1115	17	-1.9165933517244653
1115	18	-1.7558709006856077
1115	19	-1.8344973625319057
1115	20	-1.8916229143612497
1115	21	-1.8460610117548697
1115	22	-1.9520210808442755
1115	23	-1.8226491253974118
1115	24	-2.092947400118234
1115	25	-1.8952909222264083
1116	1	-1.501454902579134
1116	2	-1.6262921989056238
1116	3	-1.478169603696248
1116	4	-1.7446252806233034
1116	5	-1.329600946076873
1116	6	-1.4463442253460457
1116	7	-1.391338455729688
1116	8	-1.4062530642289843
1116	9	-1.8796115713508144
1116	10	-2.6009859328878733
1116	11	-1.9887536312762037
1116	12	-1.678963920785442
1116	13	-1.747416172619353
1116	14	-1.8240606039815432
1116	15	-2.55077919635778
1116	16	-2.0976177415726767
1116	17	-1.9900093055608679
1116	18	-2.1188275142721835
1116	19	-1.9928231370084575
1116	20	-2.0935847258756013
1116	21	-2.07954114237598
1116	22	-2.018651365820375
1116	23	-2.1435586442530976
1116	24	-1.9906188363992783
1116	25	-2.187596060688975
1201	1	0.9991698964616234
1201	2	1.0980239262040818
1201	3	1.0281099934077247
1201	4	1.0195162154883377
1201	5	1.033799646395316
1201	6	1.0185594722506863
1201	7	0.9820269812942328
1201	8	1.0595683611370035
1201	9	1.1608712722809855
1201	10	1.274692394160354
1201	11	1.1860649402851564
1201	12	1.2662186652317828
1201	13	1.1598906554539496
1201	14	1.4009845422501073
1201	15	1.3641097047707404
1201	16	1.3534981681847356
1201	17	1.310758350413181
1201	18	1.4076049525531218
1201	19	1.413015379978681
1201	20	1.4221753464917342
1201	21	1.497070022309444
1201	22	1.5657537168031297
1201	23	1.5262928749554376
1201	24	1.594610220796973
1201	25	1.657507855327367
1202	1	1.0259862809264533
1202	2	1.0635074964528926
1202	3	0.9286156261423599
1202	4	0.9761421517117412
1202	5	1.0304591927366715
1202	6	0.9324251658452027
1202	7	0.9639662196259806
1202	8	0.9721202826115487
1202	9	1.0468744547974893
1202	10	1.138659787201533
1202	11	1.1288647013041242
1202	12	1.1421405661364972
1202	13	1.1551138603096476
1202	14	1.3109385387908858
1202	15	1.3255446861066815
1202	16	1.3183861370564074
1202	17	1.2608987303353416
1202	18	1.4045248370066474
1202	19	1.4086004470157434
1202	20	1.4313774023833616
1202	21	1.4969897384006003
1202	22	1.4852057674334347
1202	23	1.526494266751051
1202	24	1.4746805783409656
1202	25	1.5700599716657635
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2211	11	-0.30253393777751114
2211	12	-0.3178298384279046
2211	13	-0.3543493592074114
2211	14	-0.38641709502132926
2211	15	-0.31375932945684326
2211	16	-0.29768695223361874
2211	17	-0.3656628060108389
2211	18	-0.42685337762010533
2211	19	-0.514693599277912
2211	20	-0.45847849619030967
2211	21	-0.32273708117804034
2211	22	-0.3332544244585449
2211	23	-0.4020198074337742
2211	24	-0.32949996049085684
2211	25	-0.3673588756118207
2212	1	-0.8206064265907074
2212	2	-0.7381454282717073
2212	3	-0.6790281453591547
2212	4	-0.7763880866145314
2212	5	-0.8171810243669881
2212	6	-0.8230785295178222
2212	7	-0.810120230940214
2212	8	-0.8319402926301223
2212	9	-0.9577728555351683
2212	10	-0.9826889554410414
2212	11	-1.0806205682679817
2212	12	-0.960897406248165
2212	13	-0.8358978804458914
2212	14	-1.0317231430706144
2212	15	-1.1221094545506105
2212	16	-1.1050833963056377
2212	17	-1.0871620040326562
2212	18	-1.058651471504703
2212	19	-1.0336239401316973
2212	20	-1.0986105593640545
2212	21	-1.0786073744943394
2212	22	-1.0238164358781248
2212	23	-1.1283609455874668
2212	24	-1.0393941020659128
2212	25	-1.2128649086003236
2213	1	-0.7744426118469541
2213	2	-0.7099232604928574
2213	3	-0.7264007423728742
2213	4	-0.7195015103714013
2213	5	-0.7121941312602189
2213	6	-0.7795362006630953
2213	7	-0.8568384082576301
2213	8	-0.892207925593361
2213	9	-0.8625457411488071
2213	10	-0.9879627593978242
2213	11	-0.974804745333266
2213	12	-0.847097067823448
2213	13	-0.9616290119434154
2213	14	-1.018431551681381
2213	15	-1.075256580828011
2213	16	-1.114085239640957
2213	17	-1.0705256038412756
2213	18	-1.0136598157646972
2213	19	-1.020649276729273
2213	20	-1.0766491469885935
2213	21	-1.049288814439663
2213	22	-1.04292613982647
2213	23	-1.0605992432608389
2213	24	-1.0066763881427363
2213	25	-1.100219892128699
2214	1	-1.1624270456642485
2214	2	-1.1400195113875142
2214	3	-1.130461639872017
2214	4	-1.157698325539016
2214	5	-1.1789742208540872
2214	6	-1.1850406366913333
2214	7	-1.2702303213375927
2214	8	-1.3150329520479218
2214	9	-1.3821835771092381
2214	10	-1.67497829373184
2214	11	-1.5894865536721312
2214	12	-1.6088778519787041
2214	13	-1.581229063660487
2214	14	-1.7640721747789954
2214	15	-1.687584888126409
2214	16	-1.7611797306421195
2214	17	-1.7355444063966416
2214	18	-1.7932108678204557
2214	19	-1.860294747015189
2214	20	-1.8963616024748546
2214	21	-1.7638842205381238
2214	22	-1.648761448623418
2214	23	-1.7955280736628865
2214	24	-1.7898384054339473
2214	25	-1.669884997065708
2215	1	-1.9663995351865324
2215	2	-1.6901576449848044
2215	3	-1.7607913053317752
2215	4	-2.466770751032435
2215	5	-1.9696387977940937
2215	6	-1.788595483725814
2215	7	-1.8155203718598472
2215	8	-1.8972334899448349
2215	9	-2.0604891056758987
2215	10	-2.106976283047544
2215	11	-2.211316111331932
2215	12	-2.120451337364109
2215	13	-2.3296545067109804
2215	14	-2.4479111987965894
2215	15	-2.4385286582417365
2215	16	-2.367903691918329
2215	17	-2.4610356268014044
2215	18	-2.6898677468115153
2215	19	-3.169509004591994
2215	20	-2.7448704766079928
2215	21	-2.7429346628157347
2215	22	-3.508182913536345
2215	23	-2.7565529452310287
2215	24	-2.80082950860011
2215	25	-2.6302263490348636
2216	1	-3.152707197652955
2216	2	-5.958886663663364
2216	3	-3.5963203140155855
2216	4	-6.237730736440486
2216	5	-3.733476744270142
2216	6	-2.64446436972554
2216	7	-2.385292660914164
2216	8	-2.298012022823258
2216	9	-2.6182910267817903
2216	10	-2.6706077773270422
2216	11	-2.501751793599709
2216	12	-2.710285921945143
2216	13	-2.5696421109499497
2216	14	-3.3411319635359646
2216	15	-3.102730151754564
2216	16	-3.1414871796494666
2216	17	-3.023019505200054
2216	18	-2.874378441166158
2216	19	-2.9981045137553224
2216	20	-3.381353713289883
2216	21	-3.509408367980491
2216	22	-3.303764254293604
2216	23	-3.3330388067191166
2216	24	-3.3537034461664206
2216	25	-3.6746977300892567
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125172.0
3	11.0
4	838.0
5	5110.0
6	7997.0
7	7559.0
8	6789.0
9	5871.0
10	5483.0
11	5555.0
12	5418.0
13	4773.0
14	4558.0
15	4437.0
16	4533.0
17	4801.0
18	5761.0
19	7440.0
20	9831.0
21	12815.0
22	15716.0
23	18082.0
24	20777.0
25	23208.0
26	25871.0
27	28579.0
28	32432.0
29	38316.0
30	48134.0
31	61687.0
32	79887.0
33	105524.0
34	144562.0
35	203374.0
36	303062.0
37	536291.0
38	1590512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04186056223494	17.922543600938535	12.995343646017558	25.04025219080897
2	16.34776510664867	21.168905921457362	37.98041828499022	24.502910686903746
3	18.254262980358348	24.884934398688603	29.412455151822307	27.448347469130734
4	12.784862732627255	16.976415394885862	37.61393504776676	32.624786824720125
5	15.233200836772042	35.68388433073162	33.3222536815444	15.760661150951933
6	31.75668965105485	37.02984717324174	16.782239235504537	14.431223940198873
7	28.2422535534751	31.57863005675799	21.722261936547035	18.456854453219876
8	26.2832534396299	32.70112099970519	20.354967451652563	20.66065810901235
9	27.129470277369883	15.132812401335013	19.383314408790785	38.35440291250432
10	16.010271343359577	26.926515466568624	31.670396299698904	25.3928168903729
11	34.71939045711984	23.023396064804118	23.17880148378591	19.07841199429013
12	24.396109611093152	24.894808968263856	28.768778371724924	21.940303048918064
13	28.894462120333575	21.402089033664055	24.986106789416816	24.717342056585554
14	23.491756724324237	21.190276873469134	26.011045771223117	29.30692063098351
15	24.32414820411581	27.847651767923747	23.457518071659685	24.37068195630076
16	24.27114849154634	27.319430490570372	24.351494101099405	24.057926916783877
17	23.906440900614555	26.092784014183483	25.446477783187426	24.554297302014533
18	25.10067110240563	25.1744788743554	26.335049390346654	23.389800632892317
19	26.10818473707419	25.458707694851725	25.757594026751917	22.67551354132216
20	25.260346470291207	25.337968859757527	25.742730579977614	23.658954089973648
21	25.310425996095994	25.393414858192674	25.71991617866575	23.576242967045584
22	24.474179585797373	25.328347980963873	26.16824817822099	24.02922425501777
23	23.706027348736434	25.055379799068284	26.339025700188106	24.89956715200718
24	23.812730747270216	26.037951852709863	26.20476592778739	23.944551472232533
25	24.062637974006197	25.456545041892426	26.20453998712769	24.276276996973685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17953.0
1	17953.0
2	14773.0
3	11593.0
4	11593.0
5	11593.0
6	9782.0
7	7971.0
8	7971.0
9	7971.0
10	9885.0
11	11799.0
12	11799.0
13	11799.0
14	15145.5
15	18492.0
16	18492.0
17	18492.0
18	30554.5
19	42617.0
20	42617.0
21	42617.0
22	61298.0
23	79979.0
24	79979.0
25	79979.0
26	108737.5
27	137496.0
28	137496.0
29	137496.0
30	163495.5
31	189495.0
32	189495.0
33	189495.0
34	221096.0
35	252697.0
36	252697.0
37	252697.0
38	279920.5
39	307144.0
40	307144.0
41	307144.0
42	336898.5
43	366653.0
44	366653.0
45	366653.0
46	390314.5
47	413976.0
48	413976.0
49	413976.0
50	422311.5
51	430647.0
52	430647.0
53	430647.0
54	401557.0
55	372467.0
56	372467.0
57	372467.0
58	335943.0
59	299419.0
60	299419.0
61	299419.0
62	260259.5
63	221100.0
64	221100.0
65	221100.0
66	182462.5
67	143825.0
68	143825.0
69	143825.0
70	112868.5
71	81912.0
72	81912.0
73	81912.0
74	64948.5
75	47985.0
76	47985.0
77	47985.0
78	39186.5
79	30388.0
80	30388.0
81	30388.0
82	22680.0
83	14972.0
84	14972.0
85	14972.0
86	11077.0
87	7182.0
88	7182.0
89	7182.0
90	4949.5
91	2717.0
92	2717.0
93	2717.0
94	1477.5
95	238.0
96	238.0
97	238.0
98	143.5
99	49.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4182278169493495
2	0.7314073338980723
3	0.7471873659480581
4	0.7560743154058117
5	0.7662715202323368
6	0.7676957108505665
7	0.784899933518782
8	0.7741615362573296
9	0.7620559160023767
10	0.755077381973051
11	0.7683508385349522
12	0.772025250329985
13	0.7704871244622968
14	0.781652778909218
15	0.7768390146196016
16	0.7709428654601304
17	0.7769814336814245
18	0.7802570721033529
19	0.7833618076510939
20	0.7757566297497469
21	0.7737912466965898
22	0.7831624209645416
23	0.7717688960187036
24	0.7760984354981221
25	0.7781777538007375
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3510766.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.61679410342332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4537616605119	50.38805379163571
2	7.3560991638039726	8.476697018505158
3	1.8017643377670736	3.1143565461604905
4	0.8510655548467468	1.9614267536848293
5	0.5024308669803145	1.4474227907004626
6	0.3177411197383874	1.0984334804494704
7	0.23584768607767034	0.9512151297954167
8	0.18720871352151455	0.8629092721068692
9	0.1500766102146817	0.778223983543113
>10	0.9798427010315959	10.710132144961793
>50	0.0830552082268201	3.3057475408254753
>100	0.06703760810027383	7.858335955907786
>500	0.009212290140984195	3.564176474541651
>1k	0.004606145067095982	4.186028702003894
>5k	1.502003826226951E-4	0.545119524103917
>10k+	1.0013358841513007E-4	0.7517208910739255
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13632	0.38829133015416006	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	12427	0.35396833625482305	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7990	0.22758566079311465	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5488	0.15631916225689776	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5419	0.15435377920374072	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4309	0.12273674747904019	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3792	0.10801061648654453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8483812364595076E-5
2	0.0	0.0	0.0	0.0	2.8483812364595076E-5
3	0.0	0.0	0.0	0.0	2.8483812364595076E-5
4	0.0	0.0	0.0	0.0	2.8483812364595076E-5
5	0.0	0.0	0.0	0.0	2.8483812364595076E-5
6	0.0	0.0	0.0	0.0	2.8483812364595076E-5
7	2.8483812364595076E-5	0.0	0.0	0.0	2.8483812364595076E-5
8	5.696762472919015E-5	0.0	0.0	0.0	2.8483812364595076E-5
9	5.696762472919015E-5	0.0	0.0	0.0	5.696762472919015E-5
10	5.696762472919015E-5	0.0	0.0	0.0	5.696762472919015E-5
11	5.696762472919015E-5	0.0	0.0	5.696762472919015E-5	5.696762472919015E-5
12	5.696762472919015E-5	0.0	0.0	5.696762472919015E-5	1.139352494583803E-4
13	1.139352494583803E-4	0.0	0.0	8.545143709378523E-5	1.139352494583803E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2550	0.0	14.427087	1
GTATCAA	8080	0.0	13.047522	1
CCGTCGT	435	0.0	12.447486	9
CGTCGTA	450	0.0	12.243667	10
GTCGTAG	435	0.0	12.229284	11
ACCGTCG	465	0.0	12.052826	8
ATACCGT	490	0.0	12.019817	6
TACCGTC	475	0.0	11.79942	7
AGTCGTA	85	5.328915E-5	11.176083	17
CCGACCA	445	0.0	11.100419	9
CGACCAT	440	0.0	11.010664	10
CGAACGA	225	0.0	10.977396	16
ATCGCCA	355	0.0	10.97145	16
CGTTATT	245	0.0	10.857545	2
TAGATCG	120	1.5256683E-6	10.291457	5
GTATTAG	510	0.0	10.251805	1
ACACCGT	205	3.6379788E-12	10.194621	6
GCGTTAT	245	0.0	10.088232	1
CATCGCC	400	0.0	9.974654	15
CGGTTCT	545	0.0	9.761128	12
>>END_MODULE