FastQCFastQC Report
Thu 2 Feb 2017
SRR4050238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510766
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT231640.6597990296134804No Hit
TATCAACGCAGAGTACTTTTTTTTT160240.45642460933027157No Hit
GGTATCAACGCAGAGTACTTTTTTT93950.26760541716537073No Hit
ACGCAGAGTACTTTTTTTTTTTTTT83760.23858041236584837No Hit
GTACTTTTTTTTTTTTTTTTTTTTT81520.23220003839617906No Hit
GAGTACTTTTTTTTTTTTTTTTTTT57020.16241469810292114No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40420.1151315695776933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29050.015.7702591
GTATCAA86650.012.8432141
CTCGACG1501.4551915E-1112.02400613
CGTTATT2250.011.41620352
ACGCGCA752.0888494E-411.39197519
CGAGCCG5450.011.14731615
CTTATAC2000.010.9399043
GTTCGAC1053.5026424E-610.84857416
TCGCACG3250.010.80730918
CGTCGTA3900.010.70979410
AGGCCCG6250.010.63190510
GCGTTAT2350.010.5596421
TCGCGTA2450.010.4614229
GAGCGTT2200.010.3558987
AGCGTTC2750.010.3557518
CCGTCGT4250.010.2745299
ATACCGT4850.010.1827496
ACGCTTA1804.0017767E-1010.0202917
ACCGCCG951.6578443E-49.9923928
CGACCGT1054.1401152E-59.94537519
Produced by FastQC (version 0.11.5)