##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3510766
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.77200531166133	31.0	31.0	34.0	27.0	34.0
2	30.833059793788593	31.0	31.0	34.0	27.0	34.0
3	31.007343411665715	31.0	31.0	34.0	27.0	34.0
4	34.573157538839105	37.0	35.0	37.0	31.0	37.0
5	34.45497392876654	37.0	35.0	37.0	31.0	37.0
6	34.46953513848545	37.0	35.0	37.0	31.0	37.0
7	34.36150543784462	37.0	35.0	37.0	30.0	37.0
8	34.32902306790028	37.0	35.0	37.0	30.0	37.0
9	35.93111902074932	39.0	37.0	39.0	31.0	39.0
10	35.82815459646128	39.0	37.0	39.0	30.0	39.0
11	35.81247710613581	39.0	37.0	39.0	30.0	39.0
12	35.72281747060328	39.0	37.0	39.0	30.0	39.0
13	35.715301731872756	39.0	37.0	39.0	30.0	39.0
14	36.88187819980027	40.0	37.0	41.0	31.0	41.0
15	36.8443795456604	40.0	37.0	41.0	31.0	41.0
16	36.82013070651818	40.0	37.0	41.0	31.0	41.0
17	36.82708132641139	40.0	37.0	41.0	31.0	41.0
18	36.81125657477599	40.0	37.0	41.0	30.0	41.0
19	36.846092562136015	40.0	37.0	41.0	30.0	41.0
20	36.81928131923347	40.0	37.0	41.0	30.0	41.0
21	36.743837099937736	40.0	37.0	41.0	30.0	41.0
22	36.67975849145172	40.0	37.0	41.0	30.0	41.0
23	36.583129436709825	40.0	37.0	41.0	30.0	41.0
24	36.519419978432055	40.0	37.0	41.0	30.0	41.0
25	36.243758769453734	40.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9233666455127576
1101	2	0.9875743559718444
1101	3	0.9164390509461953
1101	4	0.7631591619896909
1101	5	0.8799119238792201
1101	6	0.7847380051930344
1101	7	0.8661342726814993
1101	8	0.8528361986275499
1101	9	0.936954996611469
1101	10	0.8916069500383017
1101	11	0.964584375188096
1101	12	0.9654395093511425
1101	13	1.0180697312731226
1101	14	1.2037760686132302
1101	15	1.255186196059725
1101	16	1.2371945827484865
1101	17	1.1828528380608105
1101	18	1.2134503625413444
1101	19	1.2224246682635282
1101	20	1.2873693804628061
1101	21	1.2106966845722766
1101	22	1.2059883433325211
1101	23	1.3281518443382154
1101	24	1.3638446840882636
1101	25	1.272577507033752
1102	1	0.7757133395860691
1102	2	0.8745989809745076
1102	3	0.8238006531203652
1102	4	0.723003180144957
1102	5	0.8167307531630286
1102	6	0.7538137357227654
1102	7	0.8651720895913328
1102	8	0.870802959873366
1102	9	0.8445034622818568
1102	10	0.889153137285362
1102	11	0.9115839867087203
1102	12	1.0754201509485952
1102	13	1.0047614442048953
1102	14	1.140109708468259
1102	15	1.1912051184474493
1102	16	1.156841640222126
1102	17	1.2412657113165508
1102	18	1.1805541914399527
1102	19	1.125201558166502
1102	20	1.133139790792093
1102	21	1.1374773379716387
1102	22	1.1707612959888465
1102	23	1.2205446969733984
1102	24	1.2088709187396418
1102	25	1.264771858183039
1103	1	0.8524615217862497
1103	2	0.8977453653211143
1103	3	0.8594146196167785
1103	4	0.7570249155856956
1103	5	0.7695573066693271
1103	6	0.8697254187649577
1103	7	0.8972393427969081
1103	8	0.882620034543173
1103	9	0.9412914925742513
1103	10	0.9867254208015481
1103	11	0.9315518285343103
1103	12	1.016386389027673
1103	13	1.0047410031195696
1103	14	1.1213317505437814
1103	15	1.1694814706368035
1103	16	1.196320035521083
1103	17	1.2191153905348742
1103	18	1.1927985865359787
1103	19	1.240193808590334
1103	20	1.2872572959548592
1103	21	1.1984030102081746
1103	22	1.2261811480194424
1103	23	1.2971737936651948
1103	24	1.3401649347090299
1103	25	1.295991037581686
1104	1	0.6964368052687604
1104	2	0.728177193922015
1104	3	0.7183541811517422
1104	4	0.5800008440568973
1104	5	0.6342270596462427
1104	6	0.5846561092864135
1104	7	0.6565765325797059
1104	8	0.6683736038830332
1104	9	0.6935761289855265
1104	10	0.7908187148108254
1104	11	0.8683083731558199
1104	12	0.8559057238254439
1104	13	0.7434273096148161
1104	14	0.9627859642359695
1104	15	0.9430054041280869
1104	16	0.9337795646383711
1104	17	0.9137463718373482
1104	18	0.8261484506646966
1104	19	0.9187696362553552
1104	20	0.9130180605057419
1104	21	0.9116369332832264
1104	22	1.0196516552818196
1104	23	1.01091545246382
1104	24	1.0009603359109107
1104	25	0.9713605331123105
1105	1	0.5602974116558883
1105	2	0.6330495503477067
1105	3	0.585178651238504
1105	4	0.5595957351592205
1105	5	0.5135822957665255
1105	6	0.3979953395922067
1105	7	0.5682653822009698
1105	8	0.6140363075738904
1105	9	0.7406257342277414
1105	10	0.6773869533866517
1105	11	0.7123485558745628
1105	12	0.6919924093206333
1105	13	0.6941825638848798
1105	14	0.8570027181247042
1105	15	0.7946413325867994
1105	16	0.7869831755029182
1105	17	0.7912844582151592
1105	18	0.8551574581603916
1105	19	0.8091629723760221
1105	20	0.8081702042389125
1105	21	0.8376918894338843
1105	22	0.7569706209244416
1105	23	0.8297160058068727
1105	24	0.8568572844734845
1105	25	0.8171361300937079
1106	1	0.5693106588472325
1106	2	0.6134329059033732
1106	3	0.5825133628848747
1106	4	0.5539241883417461
1106	5	0.49131242449627166
1106	6	0.46590958831559703
1106	7	0.5596093417917558
1106	8	0.6291050988735094
1106	9	0.6461917813365119
1106	10	0.6054607003504913
1106	11	0.6842669225272218
1106	12	0.6502892133637204
1106	13	0.6716835235881646
1106	14	0.7700515514618402
1106	15	0.8192114377037925
1106	16	0.7319695811560649
1106	17	0.7512938832166185
1106	18	0.6921486946100188
1106	19	0.7268660477176567
1106	20	0.7927636515047141
1106	21	0.787424467581566
1106	22	0.9129327461810846
1106	23	0.8419112154474391
1106	24	0.9296109892374389
1106	25	0.869189547197962
1107	1	0.5398238161867965
1107	2	0.515437460245856
1107	3	0.4900783335265011
1107	4	0.4296904042977232
1107	5	0.3240104871914866
1107	6	0.428485014190521
1107	7	0.5497234940005882
1107	8	0.5511381784767693
1107	9	0.5861519210144834
1107	10	0.6434061266947992
1107	11	0.6663652970070686
1107	12	0.6233575794442316
1107	13	0.6449737931803554
1107	14	0.7605586786615319
1107	15	0.8201683850114776
1107	16	0.870695091962375
1107	17	0.7361052644901562
1107	18	0.7333755863346738
1107	19	0.8156968227278156
1107	20	0.9202723754456983
1107	21	0.8515457953009076
1107	22	0.8158673672889023
1107	23	0.8809943375738598
1107	24	0.880409919740373
1107	25	0.7920543663964708
1108	1	0.336980231326681
1108	2	0.3724630113340943
1108	3	0.35163867839769125
1108	4	0.24786160721104977
1108	5	0.2033972627956544
1108	6	0.1756966008431604
1108	7	0.2853408300274367
1108	8	0.3160327380910033
1108	9	0.4268579398805272
1108	10	0.3981800795418593
1108	11	0.43736106290421617
1108	12	0.43984904575161465
1108	13	0.47605372025257964
1108	14	0.4638108437384858
1108	15	0.4831691836612322
1108	16	0.5480584565066522
1108	17	0.5327875048808295
1108	18	0.5368903124955295
1108	19	0.5383350509629992
1108	20	0.5352572230549129
1108	21	0.527388808805199
1108	22	0.5180619483303275
1108	23	0.5344622435520918
1108	24	0.509989577708204
1108	25	0.5378963779563861
1109	1	0.15923677538288672
1109	2	0.2150589766143618
1109	3	0.12090202656706239
1109	4	0.1707577037987562
1109	5	0.03956833764090106
1109	6	0.04679040892965247
1109	7	0.07467198058763103
1109	8	0.10351061193353672
1109	9	0.2265954614098291
1109	10	0.21748756065575492
1109	11	0.21407537541593769
1109	12	0.22142538312648696
1109	13	0.26899340409153183
1109	14	0.24259630101240504
1109	15	0.23617001925407521
1109	16	0.22140191927161368
1109	17	0.22913490686919857
1109	18	0.16309310576303915
1109	19	0.18827110253101154
1109	20	0.20866671470750475
1109	21	0.2345494984250891
1109	22	0.19399608997184714
1109	23	0.24905091018464987
1109	24	0.22640699896636818
1109	25	0.2178880561337735
1110	1	0.17419623975037624
1110	2	0.1883643597842557
1110	3	0.10433904395301141
1110	4	0.09720534856323582
1110	5	0.08679501820369495
1110	6	0.11324604396683924
1110	7	0.1376388667499313
1110	8	0.16599533408996336
1110	9	0.15306006576500408
1110	10	0.11310127992241803
1110	11	0.24933540668222065
1110	12	0.1766380728165089
1110	13	0.24908606598930305
1110	14	0.1969407986200622
1110	15	0.2094462837226274
1110	16	0.14363259497076086
1110	17	0.10853112017473876
1110	18	0.10239188827286938
1110	19	0.24257179575823073
1110	20	0.22609558391021523
1110	21	0.2290594231017664
1110	22	0.14979475404877718
1110	23	0.2390248865296556
1110	24	0.31459690216721725
1110	25	0.2249020627975966
1111	1	-0.1207826450620999
1111	2	-0.12884575616337557
1111	3	-0.11233201273257976
1111	4	-0.19326477498402284
1111	5	-0.1724854891042611
1111	6	-0.1404431716193173
1111	7	-0.17811431805905897
1111	8	-0.0830716955823263
1111	9	-0.13153222419304456
1111	10	-0.19885849026557167
1111	11	-0.10132916621568455
1111	12	-0.18806632113224708
1111	13	-0.066807803951896
1111	14	-0.15134834307352918
1111	15	-0.2391046959382166
1111	16	-0.24006654161988905
1111	17	-0.2675278349306325
1111	18	-0.27698656393648236
1111	19	-0.23845112393890133
1111	20	-0.2804619648551494
1111	21	-0.2379476343832252
1111	22	-0.28043054815033486
1111	23	-0.26581154107309146
1111	24	-0.14163688818096887
1111	25	-0.30059561998825757
1112	1	-0.21463151193620078
1112	2	-0.35427829938195643
1112	3	-0.20886432607343153
1112	4	-0.22045600240350893
1112	5	-0.30848396703382974
1112	6	-0.25015654343148697
1112	7	-0.2113766984221499
1112	8	-0.2605450806576215
1112	9	-0.2537490361978243
1112	10	-0.2777839184372368
1112	11	-0.29965329408389607
1112	12	-0.2625712289570785
1112	13	-0.2105056597736663
1112	14	-0.3117221631321456
1112	15	-0.30519697530743173
1112	16	-0.3278841219183164
1112	17	-0.3748507093452531
1112	18	-0.3111516563398453
1112	19	-0.34633913848654885
1112	20	-0.4006154036318179
1112	21	-0.41134755646510257
1112	22	-0.41031030843524263
1112	23	-0.4633228899282784
1112	24	-0.49365974893104436
1112	25	-0.46351668100551535
1113	1	-0.3916984128661163
1113	2	-0.42964645721435346
1113	3	-0.3694578980470169
1113	4	-0.41411547809135385
1113	5	-0.575492906226799
1113	6	-0.5247037572934303
1113	7	-0.5303868175536195
1113	8	-0.5133736764319963
1113	9	-0.6505973049945197
1113	10	-0.5592678235390949
1113	11	-0.554390521086674
1113	12	-0.5086711799085109
1113	13	-0.5291712076853585
1113	14	-0.6054513187202915
1113	15	-0.6705266387326603
1113	16	-0.6473282595541505
1113	17	-0.7650053051896748
1113	18	-0.7523501030300679
1113	19	-0.7405884696107279
1113	20	-0.6941587323569038
1113	21	-0.7685420436773711
1113	22	-0.8310747066156452
1113	23	-0.7387910383399969
1113	24	-0.8703958035484121
1113	25	-0.804690430105417
1114	1	-0.7909415765944239
1114	2	-1.314465208039806
1114	3	-0.9666549294751619
1114	4	-0.7748622741435796
1114	5	-0.8501490091371053
1114	6	-0.8535095753148738
1114	7	-0.7859639103844813
1114	8	-0.8651092084540437
1114	9	-0.9931203098206751
1114	10	-1.0369113679719462
1114	11	-1.0575439723073927
1114	12	-1.0921410262007498
1114	13	-0.9647220520294724
1114	14	-1.189416618477118
1114	15	-1.1820077025042366
1114	16	-1.2327304267186179
1114	17	-1.2919358352136783
1114	18	-1.2789718067940754
1114	19	-1.1662743520144403
1114	20	-1.2440829307943062
1114	21	-1.252904416810452
1114	22	-1.2336103382619683
1114	23	-1.3834680751627602
1114	24	-1.3577552824264174
1114	25	-1.3368723030910559
1115	1	-1.0122830366942495
1115	2	-1.3098437564254617
1115	3	-1.173413250605094
1115	4	-1.0861332699961324
1115	5	-1.1722905514199056
1115	6	-1.232855632171443
1115	7	-1.2762439183053687
1115	8	-1.29473914542654
1115	9	-1.7134996815718964
1115	10	-1.447792143393869
1115	11	-1.590917971327059
1115	12	-1.4667002269898362
1115	13	-1.845496742617712
1115	14	-1.7814951566557156
1115	15	-1.8196906983974515
1115	16	-1.8360887197505136
1115	17	-1.8285850374291144
1115	18	-1.7946092559322082
1115	19	-1.811935668881084
1115	20	-1.9013221449551878
1115	21	-1.6816944355768513
1115	22	-1.8760156130087609
1115	23	-1.8026611008431601
1115	24	-1.8249888065278412
1115	25	-1.7395791487949808
1116	1	-0.6904316195246842
1116	2	-0.6944643546378444
1116	3	-0.7685707604249856
1116	4	-0.6901899332163595
1116	5	-0.9074940690476652
1116	6	-0.9329741883721212
1116	7	-0.9657300933014596
1116	8	-1.033483063247651
1116	9	-1.0208279757084426
1116	10	-0.996641215912554
1116	11	-1.0262220267936755
1116	12	-1.2100228646543911
1116	13	-1.1874658917634022
1116	14	-1.2876971922963136
1116	15	-1.3430106859767932
1116	16	-1.4997763428961974
1116	17	-1.5132096736025886
1116	18	-1.4739106674121274
1116	19	-1.4551937037883746
1116	20	-1.3346100843369797
1116	21	-1.3793159702358366
1116	22	-1.3829649024546598
1116	23	-1.567369893854547
1116	24	-1.5300626663339898
1116	25	-1.7474847281437604
1201	1	0.7267720386903704
1201	2	0.7969855672189325
1201	3	0.810293881697195
1201	4	0.7991246695708156
1201	5	0.8231627994542094
1201	6	0.8203570173993384
1201	7	0.8082059446137748
1201	8	0.8560661359093373
1201	9	0.9574472215300105
1201	10	0.9501527114941979
1201	11	0.9517189663047319
1201	12	0.95768986808325
1201	13	0.9786469020004986
1201	14	1.2048155547376922
1201	15	1.2036019896954215
1201	16	1.2124309500889439
1201	17	1.184502481679381
1201	18	1.1984131755994127
1201	19	1.2949096181020892
1201	20	1.2816126727244779
1201	21	1.3034889600353878
1201	22	1.3261400665847773
1201	23	1.309792881032024
1201	24	1.3009513978457363
1201	25	1.372813225649388
1202	1	0.6781772799045456
1202	2	0.7743386846898801
1202	3	0.7229643778283652
1202	4	0.7605522460802092
1202	5	0.8119522817944755
1202	6	0.8316791920488029
1202	7	0.8721503242335089
1202	8	0.8297647766170257
1202	9	0.976847087619575
1202	10	1.0362913049045233
1202	11	1.0421521642466516
1202	12	1.0367523702754653
1202	13	1.0343221364412045
1202	14	1.214817624191845
1202	15	1.181275365914395
1202	16	1.2243216400395056
1202	17	1.266702097096072
1202	18	1.1996171419362867
1202	19	1.232821316714734
1202	20	1.2588534511822047
1202	21	1.2428582402940407
1202	22	1.223770483117967
1202	23	1.3164986334910154
1202	24	1.2967033657758265
1202	25	1.3723207072522854
1203	1	0.4576037709739005
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2211	7	-0.29816537821199773
2211	8	-0.29502708528340094
2211	9	-0.2507698902196722
2211	10	-0.433972424200455
2211	11	-0.37969514982628283
2211	12	-0.39672976344375144
2211	13	-0.36098462196196834
2211	14	-0.45366710819439504
2211	15	-0.367732535101311
2211	16	-0.34763462670832723
2211	17	-0.38874780603238435
2211	18	-0.3256725827384983
2211	19	-0.33784729717229567
2211	20	-0.4290313985000367
2211	21	-0.46451761328353314
2211	22	-0.4623267817849879
2211	23	-0.491069961973956
2211	24	-0.4295008093565329
2211	25	-0.3921540683234426
2212	1	-0.5596863107810321
2212	2	-0.5880581210220619
2212	3	-0.6243039679669806
2212	4	-0.5662411249245523
2212	5	-0.5872489732279504
2212	6	-0.6616699328428837
2212	7	-0.7072169583558221
2212	8	-0.7304182705483626
2212	9	-0.7105501072600191
2212	10	-0.8586805625985932
2212	11	-0.8070306701737522
2212	12	-0.8725862695749527
2212	13	-0.8238705776485631
2212	14	-0.9323169344142102
2212	15	-0.9982076724298636
2212	16	-0.9727500463709333
2212	17	-0.9216995827244574
2212	18	-0.9882684583418353
2212	19	-1.0222824007876028
2212	20	-1.0123382406938788
2212	21	-0.9760511562885483
2212	22	-0.9638805355499755
2212	23	-1.049201124533191
2212	24	-1.0110011158788126
2212	25	-0.8706623838458043
2213	1	-0.6386815967821988
2213	2	-0.564740200024616
2213	3	-0.5408313402122396
2213	4	-0.5527973566253195
2213	5	-0.5075292114582695
2213	6	-0.5503082320819885
2213	7	-0.5171505983915807
2213	8	-0.5843527809631297
2213	9	-0.6226741081700808
2213	10	-0.7427064751412686
2213	11	-0.7655032644152584
2213	12	-0.7270799952801994
2213	13	-0.6808296175548989
2213	14	-0.8851972685084775
2213	15	-0.8779227637947216
2213	16	-0.9498387884057138
2213	17	-0.884688823044776
2213	18	-0.9310784130281888
2213	19	-1.0031219712025887
2213	20	-0.9149700325737768
2213	21	-0.9515759829755055
2213	22	-0.8772685217352318
2213	23	-0.8923903257422197
2213	24	-0.9171950432050622
2213	25	-0.8576745296330017
2214	1	-0.9777506578150224
2214	2	-1.5188904649420003
2214	3	-1.0314157111173259
2214	4	-0.9072955340094069
2214	5	-0.9323816863860088
2214	6	-0.9127342368554494
2214	7	-0.8980029280373074
2214	8	-1.0108716052406024
2214	9	-1.326259721849965
2214	10	-1.2459919727853332
2214	11	-1.1456862776354768
2214	12	-1.2228795693293506
2214	13	-1.23653756149902
2214	14	-1.4845129559007901
2214	15	-1.5020628524713473
2214	16	-1.5558337046271546
2214	17	-1.428511096654752
2214	18	-1.4121544296584503
2214	19	-1.512567655059307
2214	20	-1.5243683645812922
2214	21	-1.628254369179352
2214	22	-1.6081512168299525
2214	23	-1.5208889788547069
2214	24	-1.466031106256935
2214	25	-1.4046444675654968
2215	1	-1.0849387406089193
2215	2	-1.3373106468297884
2215	3	-1.3120149420795428
2215	4	-1.1307784550164328
2215	5	-1.3401442824945988
2215	6	-1.3614660902037983
2215	7	-1.275801628991701
2215	8	-1.4297854334422269
2215	9	-1.6698179622548963
2215	10	-1.678631865844956
2215	11	-1.6565671328556846
2215	12	-1.811536015959689
2215	13	-1.7830614249615664
2215	14	-2.032492087202499
2215	15	-2.1796443812500996
2215	16	-2.014832207087295
2215	17	-1.9802925391683033
2215	18	-2.1444200500586135
2215	19	-2.095689823890922
2215	20	-2.1361579268554323
2215	21	-2.055330759964697
2215	22	-2.0601425582083976
2215	23	-2.0793874071306178
2215	24	-2.223676189941777
2215	25	-2.154896566522602
2216	1	-1.2169736040661299
2216	2	-1.2055798218426759
2216	3	-1.3481450010366913
2216	4	-1.2431522351939392
2216	5	-1.2921873048508274
2216	6	-1.2202901590127908
2216	7	-1.3312247720728934
2216	8	-1.386289678056336
2216	9	-1.5876103219732016
2216	10	-1.6266519080609072
2216	11	-1.674282132859716
2216	12	-1.8224559398839304
2216	13	-1.6180338713714946
2216	14	-1.868987262580184
2216	15	-1.8897191280115422
2216	16	-1.8087521583690744
2216	17	-1.8986785147377887
2216	18	-2.0437822259470835
2216	19	-1.9379620383876528
2216	20	-2.0730419822530664
2216	21	-2.098805629744966
2216	22	-2.157700952678553
2216	23	-2.0641382124591843
2216	24	-2.094905123157673
2216	25	-2.099411098518374
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54782.0
3	1.0
4	955.0
5	7887.0
6	9812.0
7	7962.0
8	6363.0
9	5386.0
10	4995.0
11	4692.0
12	4271.0
13	3922.0
14	3770.0
15	3849.0
16	3796.0
17	4327.0
18	4994.0
19	6395.0
20	8682.0
21	11173.0
22	13905.0
23	16415.0
24	19410.0
25	22106.0
26	24300.0
27	27256.0
28	31542.0
29	38453.0
30	48945.0
31	64091.0
32	83586.0
33	110822.0
34	151223.0
35	207720.0
36	301796.0
37	524968.0
38	1666214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20856876719699	18.5717576661177	13.595245010996054	25.62442855568926
2	16.264714469851803	21.871516774317598	36.723043945792355	25.14072481003824
3	17.81910272572994	25.298553079299502	29.684262636700936	27.19808155826962
4	13.022225564526604	17.58831659522491	36.70137915632641	32.68807868392207
5	14.904685656112832	36.19311554588761	33.18275040344394	15.719448394555618
6	31.593023600434073	37.549044082109084	16.524806445435395	14.33312587202145
7	27.88269542454617	31.84957942740601	21.68915512335182	18.578570024696
8	26.302364538165502	32.82057475484576	20.277239984971867	20.59982072201687
9	26.990866359914296	15.003426610435694	19.48075031090152	38.524956718748484
10	15.956606820881364	27.210291993881548	31.523945435227834	25.30915575000926
11	34.71634931861342	22.983847422272824	23.15335734177911	19.14644591733465
12	24.251227682386318	25.066511331138123	28.910154158168787	21.772106828306768
13	28.929748071124145	21.34769785398141	25.162607809390664	24.559946265503786
14	23.42530715309412	20.993578193604513	26.39375817396563	29.18735647933573
15	24.221798556212324	27.726671047650463	23.909888461519838	24.14164193461738
16	24.07821585497783	27.15536811834294	24.64379365525162	24.12262237142761
17	23.764415660994644	25.902626545183672	25.86901314768103	24.463944646140657
18	24.85809308091258	24.85555801150295	27.0686736061106	23.21767530147387
19	25.828144493753452	25.037456490654392	26.62518443405094	22.509214581541215
20	25.024404402961625	24.923482547469305	26.573154672499605	23.47895837706946
21	25.038276529302234	24.98364256971028	26.63758742359293	23.34049347739456
22	24.204882831359704	24.90838745108182	26.967430225632487	23.91929949192599
23	23.503494447849896	24.589171655208403	27.19234730732683	24.71498658961487
24	23.652642843856825	25.590079839483586	26.98855973035339	23.768717586306202
25	23.84006034383796	24.925531590642944	27.060667718923735	24.173740346595363
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2334.0
1	2334.0
2	2989.5
3	3645.0
4	3645.0
5	3645.0
6	7473.5
7	11302.0
8	11302.0
9	11302.0
10	12668.0
11	14034.0
12	14034.0
13	14034.0
14	16203.0
15	18372.0
16	18372.0
17	18372.0
18	31378.5
19	44385.0
20	44385.0
21	44385.0
22	65076.5
23	85768.0
24	85768.0
25	85768.0
26	116583.5
27	147399.0
28	147399.0
29	147399.0
30	172345.5
31	197292.0
32	197292.0
33	197292.0
34	227219.5
35	257147.0
36	257147.0
37	257147.0
38	280624.5
39	304102.0
40	304102.0
41	304102.0
42	334929.5
43	365757.0
44	365757.0
45	365757.0
46	389063.0
47	412369.0
48	412369.0
49	412369.0
50	420035.0
51	427701.0
52	427701.0
53	427701.0
54	398940.0
55	370179.0
56	370179.0
57	370179.0
58	335069.5
59	299960.0
60	299960.0
61	299960.0
62	260141.5
63	220323.0
64	220323.0
65	220323.0
66	182126.0
67	143929.0
68	143929.0
69	143929.0
70	112570.0
71	81211.0
72	81211.0
73	81211.0
74	64473.0
75	47735.0
76	47735.0
77	47735.0
78	39000.0
79	30265.0
80	30265.0
81	30265.0
82	22829.0
83	15393.0
84	15393.0
85	15393.0
86	11314.0
87	7235.0
88	7235.0
89	7235.0
90	4950.0
91	2665.0
92	2665.0
93	2665.0
94	1442.0
95	219.0
96	219.0
97	219.0
98	132.0
99	45.0
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4651691397262022
2	0.00544040816163766
3	0.0
4	0.006095535846023346
5	0.16925081307042394
6	0.04779583714779054
7	0.0021647697397092257
8	4.842248101981163E-4
9	2.2787049891676063E-4
10	0.0021647697397092257
11	0.0016235773047819195
12	0.0024496078633551763
13	0.004899215726710353
14	0.010909300135639914
15	0.003959249918678716
16	5.696762472919016E-4
17	0.0074342750271593145
18	3.987733731043311E-4
19	7.40579121479472E-4
20	0.003674411795032765
21	0.003674411795032765
22	0.0016235773047819195
23	0.001538125867688134
24	0.0027629297993657224
25	0.006180987283117132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3510766.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.05599339912082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.36085716656507	49.84460489837074
2	7.443887861137877	8.494368333377567
3	1.796953802758652	3.075809529261681
4	0.8035614159659208	1.8339197938055902
5	0.49038254956406513	1.398963175548567
6	0.35311920440069083	1.2088540197233169
7	0.2417009645073006	0.9653342044842775
8	0.19312998656331368	0.8815378590822974
9	0.1457779640812952	0.7485755900723687
>10	1.0051446265107096	10.79944292319159
>50	0.08403136365382786	3.310914859231718
>100	0.06679779170567568	7.732943930152822
>500	0.009736742131042909	3.7551892594751153
>1k	0.004617424100223732	3.921736561574221
>5k	2.0075756957494488E-4	0.9056152840051167
>10k+	1.0037878478747244E-4	1.1221897786432415
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	23164	0.6597990296134804	No Hit
TATCAACGCAGAGTACTTTTTTTTT	16024	0.45642460933027157	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9395	0.26760541716537073	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	8376	0.23858041236584837	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8152	0.23220003839617906	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	5702	0.16241469810292114	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4042	0.1151315695776933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.8483812364595076E-5
7	0.0	0.0	0.0	0.0	2.8483812364595076E-5
8	0.0	0.0	0.0	0.0	2.8483812364595076E-5
9	0.0	0.0	0.0	0.0	2.8483812364595076E-5
10	0.0	0.0	0.0	0.0	2.8483812364595076E-5
11	0.0	0.0	0.0	5.696762472919015E-5	2.8483812364595076E-5
12	0.0	0.0	0.0	5.696762472919015E-5	5.696762472919015E-5
13	2.8483812364595076E-5	0.0	0.0	1.139352494583803E-4	5.696762472919015E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2905	0.0	15.770259	1
GTATCAA	8665	0.0	12.843214	1
CTCGACG	150	1.4551915E-11	12.024006	13
CGTTATT	225	0.0	11.4162035	2
ACGCGCA	75	2.0888494E-4	11.391975	19
CGAGCCG	545	0.0	11.147316	15
CTTATAC	200	0.0	10.939904	3
GTTCGAC	105	3.5026424E-6	10.848574	16
TCGCACG	325	0.0	10.807309	18
CGTCGTA	390	0.0	10.709794	10
AGGCCCG	625	0.0	10.631905	10
GCGTTAT	235	0.0	10.559642	1
TCGCGTA	245	0.0	10.461422	9
GAGCGTT	220	0.0	10.355898	7
AGCGTTC	275	0.0	10.355751	8
CCGTCGT	425	0.0	10.274529	9
ATACCGT	485	0.0	10.182749	6
ACGCTTA	180	4.0017767E-10	10.02029	17
ACCGCCG	95	1.6578443E-4	9.992392	8
CGACCGT	105	4.1401152E-5	9.945375	19
>>END_MODULE