FastQCFastQC Report
Thu 2 Feb 2017
SRR4050237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182252
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT154180.48449965621830077No Hit
TATCAACGCAGAGTACTTTTTTTTT96590.3035271876645847No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN70340.22103843441688464No Hit
GTACTTTTTTTTTTTTTTTTTTTTT65850.206928929575659No Hit
GGTATCAACGCAGAGTACTTTTTTT60650.1905883003608765No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN55480.17434194400694852No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48000.15083657736722295No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43230.13584719249135518No Hit
GTACAAAGGGCAGGGACTTAATCAA35560.111744764399551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.015.0512061
TAAGTCG456.76101E-414.7789475
GATTACG903.6456186E-813.72157112
GTATCAA81550.012.5715791
ATTACGG1001.9333074E-611.39909813
GTCGACA600.005881411.08245713
ACCGCGC803.7746542E-410.6869938
ACGGTAT3400.010.6171439
CGATAAC3200.010.38996810
CGAGCCG4150.09.84290715
AATTCCG3400.09.7801865
AACGTCT1951.6370905E-109.7438986
GTATAAA3650.09.631661
GTCCTAT5900.09.5015031
GTGTACG1106.828871E-59.5015031
TAATACC2303.6379788E-129.5007524
CCGATAA3500.09.4995499
AGCCCCG5400.09.49939916
ATTCCGA3650.09.3701596
GTGTAAG2950.09.340461
Produced by FastQC (version 0.11.5)