##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050237_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3182252
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.82176490108263	31.0	30.0	34.0	26.0	34.0
2	29.904364896306138	31.0	30.0	34.0	26.0	34.0
3	30.029239356279767	31.0	30.0	34.0	26.0	34.0
4	33.38302560576598	37.0	35.0	37.0	27.0	37.0
5	33.47601824117009	37.0	35.0	37.0	28.0	37.0
6	33.533863125861814	37.0	35.0	37.0	28.0	37.0
7	33.39514249657161	37.0	35.0	37.0	28.0	37.0
8	33.3191984795673	37.0	35.0	37.0	27.0	37.0
9	34.72516507177936	39.0	35.0	39.0	27.0	39.0
10	34.6281001630292	39.0	35.0	39.0	27.0	39.0
11	34.64820133666347	39.0	35.0	39.0	27.0	39.0
12	34.5400028030464	39.0	35.0	39.0	27.0	39.0
13	34.514578355202545	39.0	35.0	39.0	27.0	39.0
14	35.341013219569035	40.0	36.0	41.0	25.0	41.0
15	35.46487833144578	40.0	36.0	41.0	26.0	41.0
16	35.49758535779065	40.0	36.0	41.0	26.0	41.0
17	35.50687154882768	40.0	36.0	41.0	26.0	41.0
18	35.33541561133436	40.0	36.0	41.0	25.0	41.0
19	35.47735880125144	40.0	36.0	41.0	25.0	41.0
20	35.44875735799679	40.0	36.0	41.0	25.0	41.0
21	35.41163490509237	40.0	36.0	41.0	25.0	41.0
22	35.342996720561416	40.0	36.0	41.0	25.0	41.0
23	35.23169943800805	40.0	36.0	41.0	25.0	41.0
24	35.17772414001154	40.0	36.0	41.0	25.0	41.0
25	34.898815367230505	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.260658642869405
1101	2	1.3258909224353843
1101	3	1.2199879589914389
1101	4	1.3589037372850754
1101	5	1.2224618206020992
1101	6	1.2990207618383423
1101	7	1.242490590813432
1101	8	1.310462516492592
1101	9	1.4140316878913097
1101	10	1.410667251503341
1101	11	1.4144629812980298
1101	12	1.400440787559802
1101	13	1.5259703543986518
1101	14	1.951047230489138
1101	15	1.8293698896558723
1101	16	1.74112108404114
1101	17	1.7467283320758256
1101	18	1.8454872238273055
1101	19	1.7494061661644622
1101	20	1.7115287714802747
1101	21	1.6823431599678358
1101	22	1.743100026052538
1101	23	1.8142966288618965
1101	24	1.7415143733914675
1101	25	1.855870868372783
1102	1	1.050570781049391
1102	2	1.0811761997086364
1102	3	1.008752568897954
1102	4	1.1384379533412385
1102	5	1.0521878139503258
1102	6	0.9544443992447214
1102	7	0.8767229514641954
1102	8	0.8647701514407515
1102	9	1.0691220523935812
1102	10	1.1323172203195782
1102	11	1.076646198162571
1102	12	1.1071084689814654
1102	13	1.111813325053994
1102	14	1.5950205549747167
1102	15	1.4639282260961792
1102	16	1.426378176209397
1102	17	1.2902873172752294
1102	18	1.5424322982179746
1102	19	1.2902083812948035
1102	20	1.3057918142174216
1102	21	1.330419173098086
1102	22	1.4717459894369824
1102	23	1.4306668502495157
1102	24	1.5094705770220074
1102	25	1.4536933067720241
1103	1	0.9267557749441337
1103	2	0.9000563789027893
1103	3	0.9206953081116964
1103	4	1.014362476899592
1103	5	0.9889284339212097
1103	6	0.9690601797571929
1103	7	0.9331379733253087
1103	8	0.9583142893110193
1103	9	1.0654775055414447
1103	10	1.0717837961621655
1103	11	0.9718338357481002
1103	12	1.1595592685374427
1103	13	1.1788106179751168
1103	14	1.6379187913004145
1103	15	1.43164111259928
1103	16	1.3696669678817628
1103	17	1.2848382478064408
1103	18	1.3413740282097137
1103	19	1.2875709113310734
1103	20	1.300028914728351
1103	21	1.370786497944387
1103	22	1.3715589731297797
1103	23	1.3585785608338838
1103	24	1.3688056016696208
1103	25	1.4653109165831708
1104	1	0.687571436013446
1104	2	0.6339684621293387
1104	3	0.6242782614697475
1104	4	0.763639315104399
1104	5	0.7094436420765149
1104	6	0.702198408590121
1104	7	0.7321592441751008
1104	8	0.7318916585918842
1104	9	0.8368690931504545
1104	10	0.9233900889086044
1104	11	0.8652427457708072
1104	12	0.8873288668571604
1104	13	0.9585135370520916
1104	14	1.2733637275144147
1104	15	1.2243626725734913
1104	16	1.1455211074766822
1104	17	1.1069108097454787
1104	18	1.2420403346295004
1104	19	1.1024101768382906
1104	20	1.116908560453851
1104	21	1.0529991954194244
1104	22	1.1509173779425552
1104	23	1.0464712343191849
1104	24	1.0550324214236753
1104	25	1.0912715895483913
1105	1	0.7123165165100538
1105	2	0.7216442476262515
1105	3	0.6330106613674431
1105	4	0.8197296521585145
1105	5	0.7676281745812048
1105	6	0.7378299049065404
1105	7	0.7740241868882549
1105	8	0.790845044602996
1105	9	0.9398355467495989
1105	10	0.9091753750841818
1105	11	0.9321728492137638
1105	12	0.9381154413999795
1105	13	0.9514554398316122
1105	14	1.3590921147947341
1105	15	1.1848706724240614
1105	16	1.2001829374179493
1105	17	1.1543581183989957
1105	18	1.1949988856042992
1105	19	1.1071001688590343
1105	20	1.0325497698140111
1105	21	1.0595134005064182
1105	22	1.0628839148784692
1105	23	0.9773711660213493
1105	24	0.9942737886447404
1105	25	1.023100582505002
1106	1	0.579641748395499
1106	2	0.4594777084960455
1106	3	0.4535991184422592
1106	4	0.6331064119466205
1106	5	0.5165037733635174
1106	6	0.5166778837022434
1106	7	0.47093917110161954
1106	8	0.5672455953555229
1106	9	0.7525418478804795
1106	10	0.6714743312862481
1106	11	0.6607321734346314
1106	12	0.7777070294476331
1106	13	0.7251944257419609
1106	14	1.0138467204288801
1106	15	0.8429163044268648
1106	16	0.8935681418829091
1106	17	0.7851358083461193
1106	18	1.0666718723569133
1106	19	0.9979495635199243
1106	20	0.9058821337360214
1106	21	0.9518679755391233
1106	22	0.9004162664647382
1106	23	1.0000163155391704
1106	24	1.0311993918470534
1106	25	0.9966239399796635
1107	1	0.3570436543242117
1107	2	0.46888774148946055
1107	3	0.43932953018472887
1107	4	0.5298413313459776
1107	5	0.39302987094524156
1107	6	0.2975113874977069
1107	7	0.3405185243385631
1107	8	0.44030180922461426
1107	9	0.4846301877168102
1107	10	0.4777639108601335
1107	11	0.44764466720936724
1107	12	0.43357797422763156
1107	13	0.2654273336300079
1107	14	0.6401083164506716
1107	15	0.6020620538890853
1107	16	0.5579848973563131
1107	17	0.5510335588869708
1107	18	0.5479261323658662
1107	19	0.5716215385031305
1107	20	0.45185643590998126
1107	21	0.44086696506315093
1107	22	0.5239112522616907
1107	23	0.47205759692543836
1107	24	0.4320393344671274
1107	25	0.3499561189235223
1108	1	0.27815620371013594
1108	2	0.27877861050740194
1108	3	0.25220915419923173
1108	4	0.41571146043358453
1108	5	0.22533390595489777
1108	6	0.24878415351399497
1108	7	0.26113110021258734
1108	8	0.24315357080190836
1108	9	0.19274602382773054
1108	10	0.3660213542284865
1108	11	0.41796766803887664
1108	12	0.35795300310643086
1108	13	0.30461576186662853
1108	14	0.6780752877333356
1108	15	0.46489096122692786
1108	16	0.49071561084424076
1108	17	0.3833003159122015
1108	18	0.5585030516996881
1108	19	0.5795559522689544
1108	20	0.5703982260961098
1108	21	0.47770302977400547
1108	22	0.45095095885814374
1108	23	0.4081329622623855
1108	24	0.4590976504365045
1108	25	0.3696830274336165
1109	1	0.20155622220660874
1109	2	0.17693689854966266
1109	3	0.1998325370016545
1109	4	0.3263839409295173
1109	5	0.19452272312510388
1109	6	0.2683544074033506
1109	7	0.27623936573775865
1109	8	0.19704002366958662
1109	9	0.225434215233264
1109	10	0.30806965742186776
1109	11	0.2301487201162331
1109	12	0.2479091090543477
1109	13	0.3105205239577984
1109	14	0.5037371931080656
1109	15	0.3786643327993531
1109	16	0.36468156591868706
1109	17	0.31834907466682694
1109	18	0.40016636241480796
1109	19	0.2876888052159714
1109	20	0.384186383428343
1109	21	0.301150781889973
1109	22	0.25605764060252767
1109	23	0.26054551220000377
1109	24	0.23732006121182536
1109	25	0.3156281082447663
1110	1	-0.18300093711266996
1110	2	-0.2037246846961942
1110	3	-0.2358948287420084
1110	4	-0.1750386165600375
1110	5	-0.22582317617668224
1110	6	-0.19041181062998902
1110	7	-0.32069150029691684
1110	8	-0.32427848097240997
1110	9	-0.3337706733635244
1110	10	-0.340472768462746
1110	11	-0.35545839537668655
1110	12	-0.297024121214875
1110	13	-0.33567805260297945
1110	14	-0.022701923142086855
1110	15	-0.11470662390701136
1110	16	-0.17289715554829854
1110	17	-0.38309268488075077
1110	18	-0.22572829862176746
1110	19	-0.1723310479636453
1110	20	-0.3801195077675814
1110	21	-0.2343250579947096
1110	22	-0.26717229025121725
1110	23	-0.1323168009802913
1110	24	-0.2638304796825679
1110	25	-0.2627995250541062
1111	1	-0.2909626742364715
1111	2	-0.306085048859444
1111	3	-0.4898004751965672
1111	4	-0.2430455037046073
1111	5	-0.3288324654157364
1111	6	-0.4031036456800692
1111	7	-0.4291876955900946
1111	8	-0.3451732121224964
1111	9	-0.5755612597363964
1111	10	-0.4504844371752057
1111	11	-0.3723193113479226
1111	12	-0.3764667375478439
1111	13	-0.5034173126992343
1111	14	-0.33024968034824553
1111	15	-0.39710712933478476
1111	16	-0.4243437009691817
1111	17	-0.5283117767738332
1111	18	-0.4276744873764713
1111	19	-0.5183536842743948
1111	20	-0.5439695112270755
1111	21	-0.4342169515244976
1111	22	-0.5941379495509267
1111	23	-0.5814920981631175
1111	24	-0.6935149147735444
1111	25	-0.6847717392336605
1112	1	-0.6207813873401058
1112	2	-0.6558472965054598
1112	3	-0.7148259009249642
1112	4	-0.5671858235050564
1112	5	-0.5558296491167667
1112	6	-0.7235842878380794
1112	7	-0.6612921090316135
1112	8	-0.6235153732687237
1112	9	-0.7503119713662159
1112	10	-0.7918224290831404
1112	11	-0.7292867745518095
1112	12	-0.6513665747069552
1112	13	-0.7865633383237167
1112	14	-0.7017647283113391
1112	15	-0.7681000635975082
1112	16	-0.904980977237301
1112	17	-0.9469042316961946
1112	18	-0.7565122032336475
1112	19	-0.7469081429882394
1112	20	-0.7244641709523734
1112	21	-0.7126944066037737
1112	22	-0.6473540281422885
1112	23	-0.9024846256141856
1112	24	-0.7413833960526546
1112	25	-0.7736296329488823
1113	1	-0.8736783684911558
1113	2	-1.0312386349260265
1113	3	-0.9481677730686151
1113	4	-0.8769542225065834
1113	5	-0.9433292103167972
1113	6	-1.0073742103341417
1113	7	-1.1433798671290205
1113	8	-1.1684028554676829
1113	9	-1.0837497628124169
1113	10	-1.2732489037594021
1113	11	-1.2165265661668911
1113	12	-1.1195830354459773
1113	13	-1.3150539328241564
1113	14	-1.178479013076867
1113	15	-1.2092514367805194
1113	16	-1.209085773107553
1113	17	-1.240254206222076
1113	18	-1.2444793117363062
1113	19	-1.276669312463703
1113	20	-1.5809062763952966
1113	21	-1.3852825484732634
1113	22	-1.3880667911020694
1113	23	-1.8210657409915427
1113	24	-1.7294419647013655
1113	25	-1.7406350057580866
1114	1	-2.5261993309016724
1114	2	-2.727137187855643
1114	3	-2.294768910077593
1114	4	-5.541412925591281
1114	5	-3.0563207329618223
1114	6	-2.513391974969668
1114	7	-2.323530690034495
1114	8	-2.618332269424009
1114	9	-3.050531014114796
1114	10	-2.8040256992157815
1114	11	-2.5546055336599025
1114	12	-2.732723877135907
1114	13	-2.7354656893483735
1114	14	-2.6241142021088777
1114	15	-3.2465203155030764
1114	16	-3.2400939848390706
1114	17	-2.975869302772942
1114	18	-2.918995364883088
1114	19	-2.9904695806981962
1114	20	-3.205491082834129
1114	21	-3.23271124815858
1114	22	-3.2403079074034693
1114	23	-3.3749084060433816
1114	24	-3.329545603039719
1114	25	-3.3513674109659135
1115	1	-2.847337888557508
1115	2	-2.251253905628051
1115	3	-1.842940693065703
1115	4	-1.8027849647679552
1115	5	-1.8019918358880673
1115	6	-1.7947959227766752
1115	7	-1.8253265003425732
1115	8	-1.7397638922367946
1115	9	-2.079077009923317
1115	10	-1.9341210228166759
1115	11	-1.9525831957029993
1115	12	-2.3340133446698133
1115	13	-2.585563516087767
1115	14	-2.8572756149517673
1115	15	-2.5450383553412905
1115	16	-2.480277243950937
1115	17	-2.464568749911379
1115	18	-2.3427215738926037
1115	19	-2.2875750075280123
1115	20	-2.4366374691833386
1115	21	-2.3773072147851053
1115	22	-2.48439555173303
1115	23	-2.5722189689047212
1115	24	-2.82264038897668
1115	25	-2.8716707696413977
1116	1	-1.715813560831318
1116	2	-1.755030783995025
1116	3	-1.79538539610785
1116	4	-1.722947237456708
1116	5	-1.7868194941433124
1116	6	-1.9934717720815485
1116	7	-2.100480053249342
1116	8	-2.0155018149323887
1116	9	-2.441943347682745
1116	10	-2.7591383621713987
1116	11	-2.415871785216126
1116	12	-2.4874575204693343
1116	13	-2.437373555349275
1116	14	-3.3208820965800996
1116	15	-3.2581197957852055
1116	16	-3.106137350904973
1116	17	-2.7465594766903365
1116	18	-2.759665457191602
1116	19	-2.6522795457125454
1116	20	-2.816203021886217
1116	21	-2.906076687925946
1116	22	-2.8534416907499107
1116	23	-3.1484070756933704
1116	24	-3.1529521691172917
1116	25	-3.060783280315256
1201	1	1.2674363951847525
1201	2	1.2650698515780654
1201	3	1.290598987928334
1201	4	1.4129327042741906
1201	5	1.3185689618885093
1201	6	1.3465561141110953
1201	7	1.3187116880590892
1201	8	1.4304009899966132
1201	9	1.512439128477034
1201	10	1.5897667610042987
1201	11	1.5455661527597329
1201	12	1.5610178820184828
1201	13	1.6200687412378159
1201	14	1.9672681888487986
1201	15	1.7930606923937802
1201	16	1.8301201558197349
1201	17	1.7348334409971713
1201	18	1.9152206090114134
1201	19	1.8939111417552468
1201	20	1.8498725328370966
1201	21	1.734547859406966
1201	22	1.8331895843719224
1201	23	1.8565910023587477
1201	24	1.8937632848724775
1201	25	1.8999881205596552
1202	1	1.0422505589429285
1202	2	1.1103482302706205
1202	3	1.073792949987066
1202	4	1.1984995409611514
1202	5	1.1442080973651017
1202	6	1.0832885490492714
1202	7	1.1256544132414632
1202	8	1.1756833380344816
1202	9	1.3522285263557023
1202	10	1.3420053299986137
1202	11	1.3560160213460506
1202	12	1.3808246883115984
1202	13	1.4063056601185764
1202	14	1.7267314199525856
1202	15	1.5993375882523324
1202	16	1.569808732327445
1202	17	1.4837405951750497
1202	18	1.637479620134087
1202	19	1.4708048455230056
1202	20	1.5140417063343463
1202	21	1.531989559647684
1202	22	1.5116407458307108
1202	23	1.6542591821020451
1202	24	1.578370560682437
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1203	1	0.8068651204398343
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2211	12	-0.20089869421446593
2211	13	-0.25608309939392626
2211	14	-0.03034432481546645
2211	15	-0.24923604547179679
2211	16	-0.27588751122832633
2211	17	-0.38142901782065053
2211	18	-0.2926048773082712
2211	19	-0.2520713418347853
2211	20	-0.2419971711761022
2211	21	-0.24921465419867417
2211	22	-0.30975774419653845
2211	23	-0.3134999706738313
2211	24	-0.2731101325373899
2211	25	-0.22327148685653242
2212	1	-0.4086902848172862
2212	2	-0.48884552654442714
2212	3	-0.5264952510164598
2212	4	-0.40961791586683916
2212	5	-0.49465515854273434
2212	6	-0.528917956974297
2212	7	-0.6178217261463672
2212	8	-0.6009124411898554
2212	9	-0.5996990811327834
2212	10	-0.7327277176787348
2212	11	-0.7508857959262656
2212	12	-0.7092127190202433
2212	13	-0.680166093506422
2212	14	-0.8066373680717973
2212	15	-0.8581884816960823
2212	16	-0.8090403943594495
2212	17	-0.8833082625300932
2212	18	-0.6569435500330698
2212	19	-0.6963540182594556
2212	20	-0.7382718658354719
2212	21	-0.7358359225339228
2212	22	-0.8923859943781096
2212	23	-0.9071310981348191
2212	24	-0.7510119440949552
2212	25	-0.7535080443214071
2213	1	-0.9235335686570885
2213	2	-0.7291418131034071
2213	3	-0.7386117449396998
2213	4	-0.6271435171157052
2213	5	-0.7187604599288022
2213	6	-0.7916617717522456
2213	7	-0.8263087811933545
2213	8	-0.7066763199619999
2213	9	-0.7150918455800479
2213	10	-0.8345505227236956
2213	11	-0.8294890158613839
2213	12	-0.8002753213886677
2213	13	-1.0288275799074782
2213	14	-0.8000227338381336
2213	15	-0.9376118179530621
2213	16	-1.0073912177854893
2213	17	-1.0160972718759353
2213	18	-1.0379956234117742
2213	19	-0.9976130417599478
2213	20	-1.000587120072538
2213	21	-1.0330324045167814
2213	22	-1.214852886640223
2213	23	-1.087923931127726
2213	24	-1.092622053260996
2213	25	-1.132683528841632
2214	1	-1.271694231947599
2214	2	-1.1470875679613535
2214	3	-1.1730353694736628
2214	4	-1.124556421743847
2214	5	-1.1110805169651172
2214	6	-1.2940660011689928
2214	7	-1.2649768957295109
2214	8	-1.3072992421107728
2214	9	-1.4164185411585848
2214	10	-1.529378341531526
2214	11	-1.4210617074084553
2214	12	-1.2973263772979848
2214	13	-1.3861217358953368
2214	14	-1.2905293825575512
2214	15	-1.5738697844415697
2214	16	-1.4464760311190261
2214	17	-1.4750909810087833
2214	18	-1.4821270646514648
2214	19	-1.5855577646597823
2214	20	-1.6487948314628795
2214	21	-1.7038361801567206
2214	22	-1.6023904375055764
2214	23	-1.6080615307206685
2214	24	-1.7135734500400943
2214	25	-1.6379292512069696
2215	1	-1.7280013898772033
2215	2	-1.8088050610820368
2215	3	-1.6912281950621448
2215	4	-1.9566225260942396
2215	5	-1.8208085574643675
2215	6	-1.52079780996538
2215	7	-1.5607634491980598
2215	8	-1.678891310789318
2215	9	-2.023764384278131
2215	10	-1.6504668597822558
2215	11	-1.8528532420204513
2215	12	-1.9407835443335983
2215	13	-1.9277022711637883
2215	14	-2.6421893082401624
2215	15	-2.3114452858768715
2215	16	-2.1272770287108287
2215	17	-2.0396169666356343
2215	18	-1.97811668320616
2215	19	-2.164430685931194
2215	20	-2.4315829846932004
2215	21	-2.1587032510355897
2215	22	-2.486821526609056
2215	23	-2.183351977821019
2215	24	-2.1379440914658545
2215	25	-2.200467838717792
2216	1	-1.9847985574459557
2216	2	-1.8832063412932243
2216	3	-1.8597748032650792
2216	4	-1.7602728100305036
2216	5	-2.3525244290764924
2216	6	-2.0240790969973688
2216	7	-2.037635043702174
2216	8	-2.652692582024418
2216	9	-2.576489700456932
2216	10	-2.6176190753132786
2216	11	-2.5366629273725962
2216	12	-2.6686688386868767
2216	13	-2.6099537438271696
2216	14	-2.7354410140503305
2216	15	-2.759594945280668
2216	16	-2.7805381838457777
2216	17	-2.819683932359453
2216	18	-3.0881366967937467
2216	19	-2.818648218698094
2216	20	-3.0322380332855943
2216	21	-3.140769980912083
2216	22	-3.36941591665758
2216	23	-2.9411859605918167
2216	24	-3.243043717672272
2216	25	-3.2514760603247765
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	122039.0
3	12.0
4	844.0
5	5401.0
6	8178.0
7	8074.0
8	7448.0
9	6148.0
10	5835.0
11	5791.0
12	5692.0
13	5443.0
14	4977.0
15	4991.0
16	4761.0
17	5136.0
18	5966.0
19	7488.0
20	10100.0
21	12941.0
22	16142.0
23	18872.0
24	22073.0
25	24990.0
26	27835.0
27	30003.0
28	33932.0
29	40061.0
30	49702.0
31	63488.0
32	80397.0
33	104962.0
34	141551.0
35	196305.0
36	285166.0
37	488886.0
38	1320622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.714569126984955	17.76183695702498	12.778902684879768	24.7446912311103
2	16.571333686516063	21.223952114747487	37.93087634123778	24.273837857498677
3	18.251685329293498	25.513205106512178	29.32389158861361	26.911217975580715
4	12.523109438392806	17.001904423316518	37.54595397817615	32.929032160114524
5	15.294153004167951	35.836097468384466	33.068609074588146	15.801140452859435
6	31.64249174833954	37.098490907986395	16.66534834156812	14.593669002105944
7	28.195497999195123	31.691274921473788	21.784095309688148	18.32913176964294
8	26.521555086909398	32.50495590149319	20.218591163594418	20.754897848002994
9	27.28273822610795	15.25119713718368	19.309521451983386	38.15654318472498
10	16.394222601980275	26.80905906436319	31.072700854803596	25.724017478852936
11	34.72191200877217	23.230396601480717	23.06041369151756	18.98727769822956
12	24.67909517081495	24.821775782006807	28.594776307805418	21.904352739372825
13	28.99153164080035	21.53675617142472	25.14968866466038	24.32202352311455
14	23.36663674168158	21.12558434385516	26.347241175767216	29.16053773869604
15	24.272782663328506	27.800629037064596	23.761587233496	24.165001066110896
16	24.225195172355317	27.419105820179475	24.200517844239236	24.155181163225972
17	24.012831006007694	26.079282303962216	25.193393658086404	24.71449303194369
18	25.19530323171723	24.78558878222021	26.529983403891578	23.489124582170984
19	26.091102093705025	25.271278788325514	25.83536404235685	22.802255075612607
20	25.295858456176628	25.200756010658054	25.749952069126756	23.753433464038558
21	25.196478229370655	25.355862710422	25.937119373851203	23.510539686356143
22	24.66715049694896	25.109652730498517	26.141546299647437	24.081650472905082
23	23.941461136709638	24.821657493802146	26.327144128080104	24.909737241408113
24	23.741250975277776	25.904105436940263	26.275834182845365	24.0788094049366
25	24.02095479723766	25.415378872477532	26.316871711972766	24.246794618312038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12450.0
1	12450.0
2	12095.5
3	11741.0
4	11741.0
5	11741.0
6	10965.0
7	10189.0
8	10189.0
9	10189.0
10	11381.0
11	12573.0
12	12573.0
13	12573.0
14	14947.5
15	17322.0
16	17322.0
17	17322.0
18	28420.5
19	39519.0
20	39519.0
21	39519.0
22	55729.0
23	71939.0
24	71939.0
25	71939.0
26	97159.5
27	122380.0
28	122380.0
29	122380.0
30	144655.0
31	166930.0
32	166930.0
33	166930.0
34	193459.0
35	219988.0
36	219988.0
37	219988.0
38	244948.0
39	269908.0
40	269908.0
41	269908.0
42	301937.0
43	333966.0
44	333966.0
45	333966.0
46	356004.5
47	378043.0
48	378043.0
49	378043.0
50	385607.5
51	393172.0
52	393172.0
53	393172.0
54	367852.0
55	342532.0
56	342532.0
57	342532.0
58	312195.5
59	281859.0
60	281859.0
61	281859.0
62	243030.5
63	204202.0
64	204202.0
65	204202.0
66	166926.5
67	129651.0
68	129651.0
69	129651.0
70	100795.0
71	71939.0
72	71939.0
73	71939.0
74	57373.5
75	42808.0
76	42808.0
77	42808.0
78	34318.5
79	25829.0
80	25829.0
81	25829.0
82	19375.5
83	12922.0
84	12922.0
85	12922.0
86	10021.5
87	7121.0
88	7121.0
89	7121.0
90	5019.5
91	2918.0
92	2918.0
93	2918.0
94	1605.5
95	293.0
96	293.0
97	293.0
98	175.5
99	58.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2254692588770468
2	0.6450463382535387
3	0.6412754238193581
4	0.6493828898528463
5	0.667043339119592
6	0.6811528439608177
7	0.6763763523441889
8	0.6716627093014632
9	0.6652207304764047
10	0.6654092761981139
11	0.6707828292668211
12	0.6707828292668211
13	0.6659120647893378
14	0.6603499659989215
15	0.6671376119804465
16	0.6742395008314866
17	0.6747108651357592
18	0.6683945834585067
19	0.6663520048066589
20	0.6740509551097776
21	0.670657132119015
22	0.6713484664319482
23	0.6749936837183228
24	0.6792045381698244
25	0.6650007604677443
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3182252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.9685876185148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.7646121563825	44.89861785954772
2	8.25842553067745	8.748742726253361
3	2.41440373507812	3.8366266736386443
4	1.1831505946794247	2.5067926376070004
5	0.7048220687742153	1.8666714752664946
6	0.4846377689790739	1.5402346877645765
7	0.34214375211630094	1.2686009918469787
8	0.24791060253781977	1.0505179577666643
9	0.2038865642447893	0.9719625008194263
>10	1.2029518573413005	11.83517876645506
>50	0.10009371745427181	3.653920329674993
>100	0.07710043935559338	8.392021433270125
>500	0.01097620020169627	3.9940477789393345
>1k	0.004523159423593999	3.828957489119557
>5k	3.0154396157293324E-4	1.114583068469327
>10k+	6.030879231458665E-5	0.49252362356080603
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	15418	0.48449965621830077	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9659	0.3035271876645847	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	7034	0.22103843441688464	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6585	0.206928929575659	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6065	0.1905883003608765	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	5548	0.17434194400694852	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4800	0.15083657736722295	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4323	0.13584719249135518	No Hit
GTACAAAGGGCAGGGACTTAATCAA	3556	0.111744764399551	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.142428695150478E-5
2	0.0	0.0	0.0	0.0	3.142428695150478E-5
3	0.0	0.0	0.0	0.0	3.142428695150478E-5
4	0.0	0.0	0.0	3.142428695150478E-5	3.142428695150478E-5
5	0.0	0.0	0.0	6.284857390300957E-5	3.142428695150478E-5
6	0.0	0.0	0.0	6.284857390300957E-5	6.284857390300957E-5
7	0.0	0.0	0.0	6.284857390300957E-5	6.284857390300957E-5
8	0.0	0.0	0.0	6.284857390300957E-5	6.284857390300957E-5
9	0.0	0.0	0.0	6.284857390300957E-5	6.284857390300957E-5
10	0.0	0.0	0.0	1.2569714780601913E-4	6.284857390300957E-5
11	0.0	0.0	0.0	1.885457217090287E-4	6.284857390300957E-5
12	0.0	0.0	0.0	2.199700086605335E-4	9.427286085451435E-5
13	3.142428695150478E-5	0.0	0.0	2.199700086605335E-4	9.427286085451435E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2765	0.0	15.051206	1
TAAGTCG	45	6.76101E-4	14.778947	5
GATTACG	90	3.6456186E-8	13.721571	12
GTATCAA	8155	0.0	12.571579	1
ATTACGG	100	1.9333074E-6	11.399098	13
GTCGACA	60	0.0058814	11.082457	13
ACCGCGC	80	3.7746542E-4	10.686993	8
ACGGTAT	340	0.0	10.617143	9
CGATAAC	320	0.0	10.389968	10
CGAGCCG	415	0.0	9.842907	15
AATTCCG	340	0.0	9.780186	5
AACGTCT	195	1.6370905E-10	9.743898	6
GTATAAA	365	0.0	9.63166	1
GTCCTAT	590	0.0	9.501503	1
GTGTACG	110	6.828871E-5	9.501503	1
TAATACC	230	3.6379788E-12	9.500752	4
CCGATAA	350	0.0	9.499549	9
AGCCCCG	540	0.0	9.499399	16
ATTCCGA	365	0.0	9.370159	6
GTGTAAG	295	0.0	9.34046	1
>>END_MODULE