FastQCFastQC Report
Thu 2 Feb 2017
SRR4050237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182252
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT241050.7574824369660228No Hit
TATCAACGCAGAGTACTTTTTTTTT170900.5370410640012168No Hit
GTACTTTTTTTTTTTTTTTTTTTTT110100.34598139933606764No Hit
GGTATCAACGCAGAGTACTTTTTTT99100.31141468368941244No Hit
ACGCAGAGTACTTTTTTTTTTTTTT92090.28938625853640754No Hit
GAGTACTTTTTTTTTTTTTTTTTTT73220.23008862905891803No Hit
GCAGAGTACTTTTTTTTTTTTTTTT46370.14571441859412768No Hit
GTACAAAGGGCAGGGACTTAATCAA35160.11048779292149082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.016.4405751
GTATCAA86800.012.9775121
TAGATCG701.0905737E-412.2155085
CGAACGT1950.012.1807074
ACACCGT1950.012.1755386
TCGAACG2250.011.4004253
GCGTTAT1900.011.0347581
GTTATTC2500.011.0204113
CGTTATT1951.8189894E-1210.7193592
TTACCGT1252.2540917E-710.63604917
CGACCAT3900.010.47293810
TCGGTCG1002.4103982E-510.44611916
CGCGCTA2550.010.42749816
CGAACGA3300.010.35978816
TACCGTT1201.5336336E-610.28719718
AGAACCG2900.010.1561895
GTAATAC1606.177288E-910.0941263
AACGTCT2450.010.0783636
GAACCGA856.616682E-410.0555626
ATTCGAA2500.09.9112191
Produced by FastQC (version 0.11.5)