##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3182252
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.58616162390659	31.0	31.0	34.0	27.0	34.0
2	30.476267121522746	31.0	30.0	34.0	26.0	34.0
3	30.72972505005889	31.0	31.0	34.0	27.0	34.0
4	34.35824598429037	37.0	35.0	37.0	30.0	37.0
5	34.28699109938496	37.0	35.0	37.0	30.0	37.0
6	34.2444108763228	37.0	35.0	37.0	30.0	37.0
7	34.15804845122259	37.0	35.0	37.0	30.0	37.0
8	34.12235423216012	37.0	35.0	37.0	30.0	37.0
9	35.670106264368755	39.0	37.0	39.0	30.0	39.0
10	35.56908126697697	39.0	35.0	39.0	30.0	39.0
11	35.55806516894325	39.0	35.0	39.0	30.0	39.0
12	35.486944779986	39.0	35.0	39.0	30.0	39.0
13	35.47136807518701	39.0	35.0	39.0	30.0	39.0
14	36.59131803515246	40.0	37.0	41.0	30.0	41.0
15	36.56482704700947	40.0	37.0	41.0	30.0	41.0
16	36.55634343226118	40.0	37.0	41.0	30.0	41.0
17	36.55796932486805	40.0	37.0	41.0	30.0	41.0
18	36.52545807183089	40.0	37.0	41.0	30.0	41.0
19	36.58219713586479	40.0	37.0	41.0	30.0	41.0
20	36.53702283791478	40.0	37.0	41.0	30.0	41.0
21	36.481902438901756	40.0	37.0	41.0	30.0	41.0
22	36.42227548289702	40.0	37.0	41.0	30.0	41.0
23	36.31589735822305	40.0	37.0	41.0	30.0	41.0
24	36.25132170550918	40.0	37.0	41.0	30.0	41.0
25	36.03587145204088	40.0	36.0	41.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9172617772853471
1101	2	1.0212896213910057
1101	3	0.9229441287061491
1101	4	0.8671447908991112
1101	5	1.0198721751651831
1101	6	1.046066887514641
1101	7	0.9891819871081111
1101	8	0.9697699141600609
1101	9	1.1831214350933408
1101	10	1.15552040510061
1101	11	1.1279153736912235
1101	12	1.159855876015321
1101	13	1.1989476703657687
1101	14	1.3504598648650799
1101	15	1.3534797538334473
1101	16	1.3480778397976962
1101	17	1.3708560831508265
1101	18	1.4343327541503967
1101	19	1.447573456381896
1101	20	1.3784734709504747
1101	21	1.338600614115471
1101	22	1.3604345402384368
1101	23	1.396523224112876
1101	24	1.3958698579872646
1101	25	1.4193781414171127
1102	1	0.7370179738468075
1102	2	0.8679388205896892
1102	3	0.8091602213521405
1102	4	0.7536711249313726
1102	5	0.7792933771595756
1102	6	0.748257846161664
1102	7	0.7468780486045432
1102	8	0.7221349203911132
1102	9	0.7420640489881905
1102	10	0.7624249006932686
1102	11	0.7926546329656858
1102	12	0.8956706843691506
1102	13	0.9030923174191585
1102	14	1.084337289302887
1102	15	1.051443801862142
1102	16	0.9540157527545219
1102	17	0.9219075517521418
1102	18	0.9542949231720925
1102	19	0.9045124992513038
1102	20	0.9451258854402624
1102	21	1.024466331245108
1102	22	1.0179190424213047
1102	23	1.1201543595324992
1102	24	1.1029867110153901
1102	25	1.123386708685672
1103	1	0.6500828752571515
1103	2	0.7793693068379888
1103	3	0.712622363889853
1103	4	0.6461110337490581
1103	5	0.7115173546204474
1103	6	0.788243072768978
1103	7	0.7418424496512088
1103	8	0.722103869881586
1103	9	0.919881361197092
1103	10	0.9260355054236626
1103	11	0.8943849507636301
1103	12	0.8929600124270038
1103	13	0.850803473888682
1103	14	0.9804115556863309
1103	15	0.9801658824800938
1103	16	0.9758116552348994
1103	17	1.0472443545911148
1103	18	1.0389137288300674
1103	19	1.0105927306575069
1103	20	0.968963774537599
1103	21	1.0503844972250462
1103	22	1.0304295018578316
1103	23	1.092090437098065
1103	24	1.0852823432919863
1103	25	1.0592509964006638
1104	1	0.45887345031689364
1104	2	0.6971254447509168
1104	3	0.5678242612234996
1104	4	0.5001862451811547
1104	5	0.5791049806661448
1104	6	0.5881219716961752
1104	7	0.6786953799890298
1104	8	0.5975500887548648
1104	9	0.6692254889098734
1104	10	0.6358497277601174
1104	11	0.7080028841440082
1104	12	0.7573386855109803
1104	13	0.768210888811673
1104	14	0.8266445582589199
1104	15	0.8381638267179881
1104	16	0.8937302800237461
1104	17	0.7609628214024298
1104	18	0.8228583921031998
1104	19	0.8097213240136796
1104	20	0.8332956004957666
1104	21	0.7821795413001738
1104	22	0.8444248677501704
1104	23	0.8682924479169998
1104	24	0.9462770505688454
1104	25	0.9849137767600453
1105	1	0.4981897248377791
1105	2	0.642607777835515
1105	3	0.5922892056608653
1105	4	0.6099936893574949
1105	5	0.6903733566624766
1105	6	0.7381722611238075
1105	7	0.6832002111267101
1105	8	0.7024262474472209
1105	9	0.8320423482249595
1105	10	0.8622631240973249
1105	11	0.8176229163867177
1105	12	0.882108527563858
1105	13	0.8692362662936972
1105	14	0.9058387204705767
1105	15	0.9217954801289991
1105	16	0.9696027058037302
1105	17	0.9250011869672932
1105	18	0.9224873519166792
1105	19	0.9060531652676787
1105	20	0.9596098069238153
1105	21	0.8947106129822444
1105	22	0.8945924033862198
1105	23	0.959947803453673
1105	24	1.0582569022294024
1105	25	1.0653209678608277
1106	1	0.37618070803133463
1106	2	0.5700341628201713
1106	3	0.5362404999713988
1106	4	0.4492199179307761
1106	5	0.47192600219451464
1106	6	0.4851073595544406
1106	7	0.5061040909743753
1106	8	0.42536251319794616
1106	9	0.46862913942529616
1106	10	0.5355494394564033
1106	11	0.4820460272746203
1106	12	0.48932915636833485
1106	13	0.5572939027688406
1106	14	0.5454701892092615
1106	15	0.6407042810965322
1106	16	0.6001325827373023
1106	17	0.46007505592692155
1106	18	0.52685648385917
1106	19	0.4817475953499084
1106	20	0.6486258885974152
1106	21	0.6192291117478419
1106	22	0.6533779603898893
1106	23	0.6617547611426176
1106	24	0.7152377984815885
1106	25	0.6849387446559732
1107	1	0.16633219308827663
1107	2	0.3760294908132238
1107	3	0.3919175561578818
1107	4	0.42107707405029515
1107	5	0.283441294490153
1107	6	0.3683551086148782
1107	7	0.3785328181439809
1107	8	0.3735185504332392
1107	9	0.4732424530360362
1107	10	0.36701095976118125
1107	11	0.41593212740641405
1107	12	0.437842150248656
1107	13	0.4449116889774487
1107	14	0.48679394718567437
1107	15	0.4565279520140777
1107	16	0.5320316125835589
1107	17	0.4920499728547014
1107	18	0.4782055083135788
1107	19	0.5061279609553182
1107	20	0.4901133730721341
1107	21	0.4437719262031692
1107	22	0.4185566521625361
1107	23	0.4185744492420511
1107	24	0.35770010687303255
1107	25	0.4339960429127885
1108	1	0.10087497205073248
1108	2	0.23246334088176468
1108	3	0.20371387602109792
1108	4	0.147106356859112
1108	5	0.19752611870308812
1108	6	0.19224007374790375
1108	7	0.2406073646346627
1108	8	0.2085132435038375
1108	9	0.22648356433126082
1108	10	0.19733845764424984
1108	11	0.22116709887796304
1108	12	0.2844139635564531
1108	13	0.30905289984423945
1108	14	0.3548323787988963
1108	15	0.39178087446418886
1108	16	0.4103295294339375
1108	17	0.3967629171418423
1108	18	0.36173567166139264
1108	19	0.28288873006149373
1108	20	0.28224972400682447
1108	21	0.30305722473084984
1108	22	0.41566441371816154
1108	23	0.5072128643023248
1108	24	0.4481974281896228
1108	25	0.4449743035431908
1109	1	0.0363048739297227
1109	2	0.11190879415052635
1109	3	0.08573652066097992
1109	4	0.10630471422889087
1109	5	0.1138296249262396
1109	6	0.11202496173904564
1109	7	0.1512055085610271
1109	8	0.16903877165231762
1109	9	0.10834758623082053
1109	10	0.1379838262924693
1109	11	0.1116580774440763
1109	12	0.13528497774213832
1109	13	0.12875972424308202
1109	14	0.21560006556065758
1109	15	0.2203185815769615
1109	16	0.21008271408108214
1109	17	0.1831931372449631
1109	18	0.18592210011176746
1109	19	0.21387238351916693
1109	20	0.1786243829271612
1109	21	0.13694838816381605
1109	22	0.1493546522949174
1109	23	0.20007919597733093
1109	24	0.1956877228429832
1109	25	0.17081614174669113
1110	1	-0.16218181850900137
1110	2	-0.004799890206300006
1110	3	-0.07422284000695001
1110	4	0.0374148441359381
1110	5	0.11784235013310962
1110	6	-0.008941334353522734
1110	7	0.12885455417428204
1110	8	0.12991657549756752
1110	9	0.10702243321536287
1110	10	0.1278180020520452
1110	11	-0.01213370201018904
1110	12	0.14095105440522104
1110	13	0.06445743531706682
1110	14	0.0776285308783713
1110	15	0.09684185001744083
1110	16	0.09083702940073124
1110	17	0.015885183636477507
1110	18	0.1093353548523126
1110	19	0.05200121983300221
1110	20	0.058466864255080964
1110	21	0.09140198071589367
1110	22	0.04818094533837325
1110	23	0.10606989895578778
1110	24	0.03545491371528442
1110	25	0.023663641910786737
1111	1	-0.4186743681760845
1111	2	-0.2806075696056851
1111	3	-0.32886855917156055
1111	4	-0.2524463622091133
1111	5	-0.2693466910377609
1111	6	-0.42671248390357874
1111	7	-0.29532572977206684
1111	8	-0.3108719524258987
1111	9	-0.28927470374353703
1111	10	-0.314445365109826
1111	11	-0.3411428696259762
1111	12	-0.2670031702823863
1111	13	-0.30659418077482314
1111	14	-0.25250903816569803
1111	15	-0.3403877659888508
1111	16	-0.2915809515175454
1111	17	-0.42338846432225097
1111	18	-0.43343124778875364
1111	19	-0.476206337328712
1111	20	-0.40127667122725086
1111	21	-0.3913946451390231
1111	22	-0.48504385097930935
1111	23	-0.45483735199037056
1111	24	-0.4188798422565583
1111	25	-0.4641886580082826
1112	1	-0.43838427659696677
1112	2	-0.31881090336907647
1112	3	-0.3528609157617346
1112	4	-0.346732053248445
1112	5	-0.3244329409200759
1112	6	-1.2224839673364656
1112	7	-0.5483279416651499
1112	8	-0.3299123650956446
1112	9	-0.3305607399916184
1112	10	-0.3976711258185617
1112	11	-0.4544322440542601
1112	12	-0.3655422515156985
1112	13	-0.3546286412089046
1112	14	-0.47098107683837753
1112	15	-0.4922831014864215
1112	16	-0.5009852746468795
1112	17	-0.5214268185250219
1112	18	-0.48162099404289194
1112	19	-0.4993346259650693
1112	20	-0.5795457225076035
1112	21	-0.5559694862790749
1112	22	-0.6780578487871765
1112	23	-0.6522651400159276
1112	24	-0.554938116282564
1112	25	-0.43530433315871875
1113	1	-0.7123310380634038
1113	2	-0.8961878946908008
1113	3	-0.6334465073732076
1113	4	-0.6687126587639511
1113	5	-0.673312506693108
1113	6	-1.2507442985853814
1113	7	-0.8471679917215482
1113	8	-0.8783648006015312
1113	9	-0.9889849879359005
1113	10	-0.8764822577706965
1113	11	-0.920908779877891
1113	12	-0.9759225037142087
1113	13	-0.8804518062422844
1113	14	-1.0412357515998352
1113	15	-0.9338392142784144
1113	16	-0.9913091896724282
1113	17	-1.0159787729163838
1113	18	-0.9821081806092522
1113	19	-1.0056043897531168
1113	20	-1.0359034834797427
1113	21	-1.0349680925554807
1113	22	-1.1013787628952798
1113	23	-1.0968874447167352
1113	24	-1.1044505027756841
1113	25	-1.1025112218913762
1114	1	-0.9869570060951958
1114	2	-1.0100612093752588
1114	3	-1.107179159625911
1114	4	-0.8956125192237607
1114	5	-1.1603562080279062
1114	6	-1.592827514547288
1114	7	-1.3887412006085356
1114	8	-1.4737898382984724
1114	9	-1.5831823958727682
1114	10	-1.6391378470857063
1114	11	-1.4504275043229313
1114	12	-1.4625736070557949
1114	13	-1.6120025250072985
1114	14	-1.7880739412228408
1114	15	-1.7037585255052505
1114	16	-1.6808868207631278
1114	17	-1.7479178216228917
1114	18	-1.8637171331741271
1114	19	-1.645122795349586
1114	20	-1.632656763979611
1114	21	-1.6556451838129504
1114	22	-1.568951361199808
1114	23	-1.594574035150977
1114	24	-1.7007236902219915
1114	25	-1.7328782969352758
1115	1	-1.2409572704572724
1115	2	-1.8724185081312221
1115	3	-1.4443448916598243
1115	4	-1.1285497670740625
1115	5	-1.3293387460826338
1115	6	-1.7741057626568448
1115	7	-1.350746176234864
1115	8	-1.3999475398553969
1115	9	-1.464532736925392
1115	10	-1.5449797700580064
1115	11	-1.5503154377905304
1115	12	-1.5590590920452598
1115	13	-1.6380458293033797
1115	14	-1.800131565769945
1115	15	-1.6642140775558403
1115	16	-1.7687988009627915
1115	17	-1.7950885407771366
1115	18	-1.9046640410956641
1115	19	-1.8602165608744698
1115	20	-1.8303709579243161
1115	21	-1.9274030447533903
1115	22	-1.9822306386612212
1115	23	-1.9201436587989917
1115	24	-1.957196282771065
1115	25	-1.9581079215794404
1116	1	-1.1760184951467458
1116	2	-1.2712966696341148
1116	3	-1.3280530943463482
1116	4	-1.1438130388410528
1116	5	-1.1642274372884174
1116	6	-1.390959504982753
1116	7	-1.4603553586205749
1116	8	-1.4276720752277612
1116	9	-1.468488019301617
1116	10	-1.664457905585401
1116	11	-1.6074273506807657
1116	12	-1.556353884431445
1116	13	-1.6206217622632337
1116	14	-1.7816191159159942
1116	15	-1.8479091031218218
1116	16	-1.84609159232037
1116	17	-1.8764354350245114
1116	18	-1.8647613529472977
1116	19	-1.8874957884774304
1116	20	-1.9013575107911649
1116	21	-2.0885738737331465
1116	22	-1.8604564332770934
1116	23	-1.9356334192294753
1116	24	-2.0051944256650245
1116	25	-2.1230944659245594
1201	1	0.8342427892550042
1201	2	0.9865769186463851
1201	3	0.9216092028134
1201	4	0.806593737564576
1201	5	0.8400962908664766
1201	6	0.972707073251506
1201	7	0.9438453158813545
1201	8	0.9277626874587597
1201	9	1.0251285460563082
1201	10	1.1443008471747333
1201	11	1.0898792448142842
1201	12	1.1818230109592704
1201	13	1.1226596763656431
1201	14	1.2849954879655243
1201	15	1.3031073078890287
1201	16	1.3393275591322507
1201	17	1.2562372904867871
1201	18	1.2470484614620503
1201	19	1.2546677530284924
1201	20	1.32240426868848
1201	21	1.3538877956325237
1201	22	1.329558331640932
1201	23	1.2651548961382275
1201	24	1.418182356106513
1201	25	1.3799392639863157
1202	1	0.7348231642447267
1202	2	0.8594607330738206
1202	3	0.8542033117379084
1202	4	0.8684820118349919
1202	5	0.8530174171398599
1202	6	0.9425143048316187
1202	7	0.9524747292971725
1202	8	0.9266112370883377
1202	9	1.0007176961344229
1202	10	1.075739082744036
1202	11	1.0956296920744393
1202	12	1.093874113444933
1202	13	1.0901748378471154
1202	14	1.2387153032208502
1202	15	1.3109622453293142
1202	16	1.289424779739953
1202	17	1.2872946390208213
1202	18	1.3862116667650994
1202	19	1.4252134529516596
1202	20	1.3557321394466015
1202	21	1.4332803156848044
1202	22	1.3683177978206942
1202	23	1.4357354128902813
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2211	7	-0.31339973069099614
2211	8	-0.2852962756887436
2211	9	-0.2440289507526927
2211	10	-0.3108168808018945
2211	11	-0.3098442097205307
2211	12	-0.2763697341233353
2211	13	-0.3181236640968734
2211	14	-0.40035260768652847
2211	15	-0.4016083947329676
2211	16	-0.34636346768709103
2211	17	-0.42764758709197537
2211	18	-0.3316186857140835
2211	19	-0.39799401962036285
2211	20	-0.4099950653441482
2211	21	-0.37513926918962426
2211	22	-0.3799695001656005
2211	23	-0.4724647927012242
2211	24	-0.5630861618383207
2211	25	-0.45532855285480167
2212	1	-0.4049462047451442
2212	2	-0.43155062515238996
2212	3	-0.4256417933865677
2212	4	-0.3898514421671493
2212	5	-0.5134557845108887
2212	6	-0.37664487488373055
2212	7	-0.45647126153638595
2212	8	-0.45011960344672985
2212	9	-0.5229231232726335
2212	10	-0.5125495921588197
2212	11	-0.5291466866764409
2212	12	-0.49523861974755334
2212	13	-0.47490733761411263
2212	14	-0.5438746882884544
2212	15	-0.624901754337202
2212	16	-0.7100296655893956
2212	17	-0.6218112928386006
2212	18	-0.6784979272830753
2212	19	-0.7442096843058223
2212	20	-0.8091816262321174
2212	21	-0.6807558366913824
2212	22	-0.7320654463274181
2212	23	-0.7306637200494492
2212	24	-0.894114719616411
2212	25	-0.805625754484403
2213	1	-0.510973157970259
2213	2	-0.8218862175795607
2213	3	-0.6017788397801844
2213	4	-0.6084837462404735
2213	5	-0.7515128823210873
2213	6	-0.6430810833867184
2213	7	-0.7114599740826719
2213	8	-0.6514755941132506
2213	9	-0.8121381995461903
2213	10	-0.7973643081903745
2213	11	-0.8032963503572788
2213	12	-0.9053271392746112
2213	13	-0.8889973140867014
2213	14	-0.9103588760677042
2213	15	-0.9503511701329046
2213	16	-1.0554949159489695
2213	17	-0.9430300426027287
2213	18	-1.0146984949521283
2213	19	-0.9485387469291666
2213	20	-1.007468392682057
2213	21	-0.9363839166780394
2213	22	-0.9299469638993045
2213	23	-1.0036304671313374
2213	24	-0.9698312342997184
2213	25	-0.9853492567885453
2214	1	-0.9277747952014188
2214	2	-0.9632315693039892
2214	3	-1.0203467789805387
2214	4	-0.9486981378849819
2214	5	-1.010446932690023
2214	6	-1.0383957281926541
2214	7	-0.9504027726346465
2214	8	-0.9926956676110592
2214	9	-1.1077878569823696
2214	10	-1.210761098480269
2214	11	-1.2003188551018056
2214	12	-1.2537008926933098
2214	13	-1.149201750522856
2214	14	-1.3886082634861694
2214	15	-1.5029393705963159
2214	16	-1.5371400895287621
2214	17	-1.4066863967319208
2214	18	-1.4513119481975494
2214	19	-1.5554425046514169
2214	20	-1.5811778213005354
2214	21	-1.6736353103149852
2214	22	-1.5280094390772163
2214	23	-1.6604401270359332
2214	24	-1.630303876294029
2214	25	-1.5785387150759078
2215	1	-1.4598844209884945
2215	2	-3.3161971380870945
2215	3	-1.805963301319565
2215	4	-1.3968915722886237
2215	5	-1.419169255110802
2215	6	-1.3420567388638318
2215	7	-1.3460205289876015
2215	8	-1.406862789796854
2215	9	-1.6608837148307813
2215	10	-1.5942083514623704
2215	11	-1.5916893229267757
2215	12	-1.6964917315259171
2215	13	-1.6891556876597065
2215	14	-1.9600602915713594
2215	15	-1.9243474611009788
2215	16	-1.918831870858348
2215	17	-1.9143558621351957
2215	18	-1.8245276004224067
2215	19	-1.7715653324870786
2215	20	-1.8200483492533763
2215	21	-1.8547848529466933
2215	22	-1.9944130329347232
2215	23	-2.0215334109527845
2215	24	-2.0910947710930685
2215	25	-2.0893519669568903
2216	1	-1.326085621392238
2216	2	-1.939483563864563
2216	3	-1.4941566734952652
2216	4	-1.3179891959789103
2216	5	-1.4536493885741564
2216	6	-1.3174526158050242
2216	7	-1.527285789460663
2216	8	-1.4831460905329337
2216	9	-1.6250396148011674
2216	10	-1.5361479218215806
2216	11	-1.676870844995932
2216	12	-1.7859976570935814
2216	13	-1.8973098582544807
2216	14	-1.905500793038847
2216	15	-1.9513292827245294
2216	16	-1.9126745709784672
2216	17	-1.9208071352928258
2216	18	-2.138950924771507
2216	19	-2.134021717127581
2216	20	-2.2589044151270485
2216	21	-2.1533412718326375
2216	22	-2.1224687443429815
2216	23	-2.275335870426332
2216	24	-2.326542740201951
2216	25	-2.4449141895646207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54898.0
3	7.0
4	898.0
5	7426.0
6	9562.0
7	7912.0
8	6456.0
9	5365.0
10	4857.0
11	4600.0
12	4245.0
13	3749.0
14	3565.0
15	3685.0
16	3773.0
17	4021.0
18	4806.0
19	6330.0
20	8742.0
21	11255.0
22	13659.0
23	16089.0
24	18820.0
25	20760.0
26	23543.0
27	26658.0
28	30867.0
29	37611.0
30	47446.0
31	62712.0
32	81041.0
33	106709.0
34	144685.0
35	198320.0
36	288543.0
37	500054.0
38	1408583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05878369260765	18.426780807147065	13.322775591122163	25.191659909123125
2	16.41751736544335	21.87973578001027	36.933254219028036	24.769492635518343
3	17.962310967201844	25.810558057627116	29.538829734414495	26.688301240756545
4	12.933136659196066	17.572618384716232	36.46568530713469	33.028559648953006
5	14.93886153843252	36.404901892049345	32.93013461370987	15.726101955808272
6	31.437295106695363	37.528251392771665	16.549785301985075	14.484668198547897
7	27.794803368050037	32.02390971101819	21.7889356149859	18.392351305945876
8	26.520673160620717	32.71055438272764	20.129455899331354	20.639316557320278
9	27.07279536711328	15.15550070444291	19.452021042462366	38.31968288598144
10	16.25778266677143	27.1170582226762	31.08776616641785	25.537392944134517
11	34.64805445765004	23.114520772491108	23.144406988032003	19.093017781826855
12	24.545028850142682	24.912161057964273	28.82016115853122	21.72264893336183
13	29.102942022294208	21.384513988175588	25.447658391381022	24.06488559814918
14	23.51157142911015	20.902610437980886	26.487768639296476	29.098049493612493
15	24.113556986565214	27.685510901460653	24.178178723104658	24.02275338886947
16	24.063200238847248	27.11789940131052	24.61163751787426	24.207262841967975
17	23.859564548153188	25.788903714998458	25.777118948207995	24.574412788640366
18	25.034763172060497	24.41658362785793	27.186638874983622	23.362014325097956
19	25.769313684390475	24.810330967825873	26.856261324990054	22.564094022793597
20	24.93886170583854	24.73047927017438	26.763834287097005	23.566824736890073
21	25.016120983423985	24.81795544449291	26.925907583084342	23.240015988998763
22	24.475937166509752	24.566533385790944	27.093077480978877	23.864451966720424
23	23.711992035603465	24.254453418776293	27.325384439887095	24.708170105733146
24	23.583603879523956	25.372555538830277	27.178602595797862	23.8652379858479
25	23.793518040743063	24.80728656338756	27.356854690454924	24.042340705414453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2008.0
1	2008.0
2	3170.0
3	4332.0
4	4332.0
5	4332.0
6	9278.5
7	14225.0
8	14225.0
9	14225.0
10	14952.5
11	15680.0
12	15680.0
13	15680.0
14	16512.0
15	17344.0
16	17344.0
17	17344.0
18	29409.5
19	41475.0
20	41475.0
21	41475.0
22	59136.0
23	76797.0
24	76797.0
25	76797.0
26	103558.0
27	130319.0
28	130319.0
29	130319.0
30	152107.5
31	173896.0
32	173896.0
33	173896.0
34	197940.5
35	221985.0
36	221985.0
37	221985.0
38	244817.5
39	267650.0
40	267650.0
41	267650.0
42	299675.5
43	331701.0
44	331701.0
45	331701.0
46	353907.0
47	376113.0
48	376113.0
49	376113.0
50	383206.5
51	390300.0
52	390300.0
53	390300.0
54	365027.0
55	339754.0
56	339754.0
57	339754.0
58	310526.0
59	281298.0
60	281298.0
61	281298.0
62	242580.5
63	203863.0
64	203863.0
65	203863.0
66	166968.5
67	130074.0
68	130074.0
69	130074.0
70	100523.0
71	70972.0
72	70972.0
73	70972.0
74	56755.0
75	42538.0
76	42538.0
77	42538.0
78	34495.5
79	26453.0
80	26453.0
81	26453.0
82	19852.0
83	13251.0
84	13251.0
85	13251.0
86	10127.0
87	7003.0
88	7003.0
89	7003.0
90	4966.5
91	2930.0
92	2930.0
93	2930.0
94	1590.0
95	250.0
96	250.0
97	250.0
98	145.5
99	41.0
100	41.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32957792154738214
2	0.011249894728638713
3	0.0
4	0.0
5	0.04779634045323878
6	0.003393822990762516
7	0.0035823687124715456
8	3.142428695150478E-4
9	0.009584407520208959
10	0.0157749920496554
11	0.005750644512125375
12	0.009175891789839397
13	0.016623447797346032
14	0.0032681258429564973
15	0.020740029387993155
16	0.009490134659354445
17	0.005593523077367851
18	1.571214347575239E-4
19	0.0040851573036956215
20	0.0038337630080835837
21	0.002011154364896306
22	0.0
23	0.0028281858256354302
24	0.0034252472777140212
25	0.0020740029387993158
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3182252.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.20125601123694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.63433793450164	44.18018741860463
2	8.405668430706369	8.775728993937507
3	2.391418559686673	3.7450515739268253
4	1.1542577811726082	2.410148237518145
5	0.6961443986652773	1.8169805987757366
6	0.46647275582330844	1.4610278249399846
7	0.3475353877043334	1.2699248622562875
8	0.263085365178204	1.0986709200381752
9	0.20835568439445787	0.9788785580224422
>10	1.2380894675814758	12.0560893929503
>50	0.10186700777328997	3.6819940286838375
>100	0.07681476814507474	8.372975026847964
>500	0.010997528323870243	4.0119694853464765
>1k	0.004592374464720588	3.6596328302177445
>5k	1.8127793939686534E-4	0.8340316468176814
>10k+	1.8127793939686534E-4	1.6467086011163363
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	24105	0.7574824369660228	No Hit
TATCAACGCAGAGTACTTTTTTTTT	17090	0.5370410640012168	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	11010	0.34598139933606764	No Hit
GGTATCAACGCAGAGTACTTTTTTT	9910	0.31141468368941244	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	9209	0.28938625853640754	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	7322	0.23008862905891803	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4637	0.14571441859412768	No Hit
GTACAAAGGGCAGGGACTTAATCAA	3516	0.11048779292149082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.427286085451435E-5
2	0.0	0.0	0.0	0.0	9.427286085451435E-5
3	0.0	0.0	0.0	0.0	9.427286085451435E-5
4	0.0	0.0	0.0	3.142428695150478E-5	9.427286085451435E-5
5	3.142428695150478E-5	0.0	0.0	6.284857390300957E-5	1.2569714780601913E-4
6	6.284857390300957E-5	0.0	0.0	6.284857390300957E-5	1.5712143475752393E-4
7	6.284857390300957E-5	0.0	0.0	6.284857390300957E-5	1.5712143475752393E-4
8	6.284857390300957E-5	0.0	0.0	9.427286085451435E-5	1.5712143475752393E-4
9	6.284857390300957E-5	0.0	0.0	1.2569714780601913E-4	1.5712143475752393E-4
10	6.284857390300957E-5	0.0	0.0	1.5712143475752393E-4	1.5712143475752393E-4
11	6.284857390300957E-5	0.0	0.0	2.199700086605335E-4	1.5712143475752393E-4
12	6.284857390300957E-5	0.0	0.0	2.199700086605335E-4	1.885457217090287E-4
13	6.284857390300957E-5	0.0	0.0	2.199700086605335E-4	2.5139429561203826E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2765	0.0	16.440575	1
GTATCAA	8680	0.0	12.977512	1
TAGATCG	70	1.0905737E-4	12.215508	5
CGAACGT	195	0.0	12.180707	4
ACACCGT	195	0.0	12.175538	6
TCGAACG	225	0.0	11.400425	3
GCGTTAT	190	0.0	11.034758	1
GTTATTC	250	0.0	11.020411	3
CGTTATT	195	1.8189894E-12	10.719359	2
TTACCGT	125	2.2540917E-7	10.636049	17
CGACCAT	390	0.0	10.472938	10
TCGGTCG	100	2.4103982E-5	10.446119	16
CGCGCTA	255	0.0	10.427498	16
CGAACGA	330	0.0	10.359788	16
TACCGTT	120	1.5336336E-6	10.287197	18
AGAACCG	290	0.0	10.156189	5
GTAATAC	160	6.177288E-9	10.094126	3
AACGTCT	245	0.0	10.078363	6
GAACCGA	85	6.616682E-4	10.055562	6
ATTCGAA	250	0.0	9.911219	1
>>END_MODULE