FastQCFastQC Report
Thu 2 Feb 2017
SRR4050236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147151
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT144080.4578108899128132No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN106160.3373209610851211No Hit
TATCAACGCAGAGTACTTTTTTTTT91160.28965880569442015No Hit
GTACTTTTTTTTTTTTTTTTTTTTT62290.19792504395245097No Hit
GGTATCAACGCAGAGTACTTTTTTT55890.17758919098575188No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN47510.15096193350748024No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44970.14289114186132157No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40660.12919621587906013No Hit
GTACAAAGGGCAGGGACTTAATCAA36330.11543774035627778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.014.7805511
GTATCAA81800.012.9203571
TAGATCG751.480036E-512.6663995
GCGTTAT1406.7666406E-1011.5410141
TATACGG600.005878833611.0830995
CCGTCCA1605.456968E-1010.6865919
TCTATAC2053.6379788E-1210.1949073
ACCGCTT750.002650722410.1324718
CCTATTA2650.010.0381672
GTCTAAT1909.822543E-1110.0045941
CCCTATT2700.09.8563781
GGTTAGG1356.4083724E-79.8563781
TTTTCGG2900.09.82753716
TCGAACG2151.0913936E-119.7207263
AACGTCT2550.09.6856066
GTTTTCG3050.09.65584415
TTTCGTG900.0011142289.49995218
TAGACCG1201.7020091E-59.49985
ATCGCCA3650.09.36981616
CGAACGA2650.09.32070816
Produced by FastQC (version 0.11.5)