##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050236_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3147151
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.90782615768992	31.0	30.0	34.0	26.0	34.0
2	29.929183569520497	31.0	30.0	34.0	26.0	34.0
3	30.081290983495865	31.0	31.0	34.0	26.0	34.0
4	33.49040672023681	37.0	35.0	37.0	28.0	37.0
5	33.54014059064849	37.0	35.0	37.0	28.0	37.0
6	33.57993404193189	37.0	35.0	37.0	28.0	37.0
7	33.45285211926596	37.0	35.0	37.0	28.0	37.0
8	33.406386919470975	37.0	35.0	37.0	28.0	37.0
9	34.83376870064385	39.0	35.0	39.0	27.0	39.0
10	34.72487402097961	39.0	35.0	39.0	27.0	39.0
11	34.75276750305276	39.0	35.0	39.0	27.0	39.0
12	34.67526534316275	39.0	35.0	39.0	27.0	39.0
13	34.65294452029788	39.0	35.0	39.0	27.0	39.0
14	35.70308383677809	40.0	36.0	41.0	27.0	41.0
15	35.691724991905375	40.0	36.0	41.0	27.0	41.0
16	35.70152242456749	40.0	36.0	41.0	27.0	41.0
17	35.68942576952933	40.0	36.0	41.0	27.0	41.0
18	35.62978674998435	40.0	36.0	41.0	27.0	41.0
19	35.67802434646447	40.0	36.0	41.0	27.0	41.0
20	35.65195092323184	40.0	36.0	41.0	27.0	41.0
21	35.57373033578624	40.0	36.0	41.0	26.0	41.0
22	35.48945125289508	40.0	36.0	41.0	26.0	41.0
23	35.40918437024471	40.0	36.0	41.0	26.0	41.0
24	35.361990257219944	40.0	36.0	41.0	25.0	41.0
25	35.03230223144679	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.2214467742782809
1101	2	1.2904899233105702
1101	3	1.2522334456405417
1101	4	1.3444885444234558
1101	5	1.358716647189155
1101	6	1.2427806501092462
1101	7	1.2141260019395403
1101	8	1.269766223357486
1101	9	1.3781757669950565
1101	10	1.440630993000191
1101	11	1.3990658525553812
1101	12	1.4612585836493466
1101	13	1.47932952309624
1101	14	1.7461341330753015
1101	15	1.6959786778341837
1101	16	1.6434692526411325
1101	17	1.6893443489517068
1101	18	1.7968730093727316
1101	19	1.7826559135971465
1101	20	1.7873498122359308
1101	21	1.769707943291742
1101	22	1.8223279948585684
1101	23	1.8784718847773973
1101	24	1.885933681685188
1101	25	1.810030956387017
1102	1	1.110113029908252
1102	2	1.141704358693076
1102	3	1.1891560227820683
1102	4	1.2220257992794927
1102	5	1.1277246197435815
1102	6	1.168515193975324
1102	7	1.1139476676204367
1102	8	1.087156349860905
1102	9	1.3112469514087124
1102	10	1.3404520611861344
1102	11	1.3516073063072085
1102	12	1.393751930692403
1102	13	1.3264900149612586
1102	14	1.6220260660748522
1102	15	1.709664359518868
1102	16	1.7207611999686065
1102	17	1.6817170271087178
1102	18	1.6575906617576948
1102	19	1.7281373072158885
1102	20	1.6230384607220643
1102	21	1.7529552602555825
1102	22	1.7631724093831238
1102	23	1.7114213773936413
1102	24	1.785927963658466
1102	25	1.588377476334415
1103	1	1.0509607331399877
1103	2	1.1165077821283518
1103	3	1.1005014035940093
1103	4	1.185235017561169
1103	5	1.1845474947783714
1103	6	1.2126954073498624
1103	7	1.0847773814346624
1103	8	1.1097099180028565
1103	9	1.2939561714686576
1103	10	1.3403345842658467
1103	11	1.3993247197432837
1103	12	1.2676709096279097
1103	13	1.446466681844278
1103	14	1.56165504350772
1103	15	1.5250717743686621
1103	16	1.6105999687304546
1103	17	1.5922960849638272
1103	18	1.536332529509579
1103	19	1.6126185949318739
1103	20	1.6754696772628108
1103	21	1.7263762459680763
1103	22	1.774574950441142
1103	23	1.6918413534937002
1103	24	1.711774686619215
1103	25	1.6988439586241881
1104	1	0.9140492053193334
1104	2	0.9912620449742988
1104	3	0.9956426011966606
1104	4	1.0222984208299195
1104	5	1.0508831205953726
1104	6	1.0462511426413528
1104	7	0.994531089338814
1104	8	1.0287715518365275
1104	9	1.0934297634686487
1104	10	1.243607061109259
1104	11	1.1325106214152498
1104	12	1.1719407401856827
1104	13	1.2086793878425013
1104	14	1.2881774976006426
1104	15	1.3157721831970406
1104	16	1.326286812315118
1104	17	1.296091759224474
1104	18	1.3133427264054731
1104	19	1.348180008915044
1104	20	1.408249100021294
1104	21	1.3586151532983237
1104	22	1.3832655621018048
1104	23	1.378350750507991
1104	24	1.4231161871648013
1104	25	1.297002264167908
1105	1	0.8054127980030188
1105	2	0.9051774138438127
1105	3	0.9056689511730802
1105	4	1.0142766251555244
1105	5	0.9192802973864858
1105	6	0.8978135955232034
1105	7	0.8657111268041504
1105	8	0.8290301563392077
1105	9	0.9567246101956997
1105	10	1.0933301219926364
1105	11	0.9161235777149415
1105	12	1.0153813812699397
1105	13	1.0509956768201363
1105	14	1.1950887725438548
1105	15	1.1390271581698954
1105	16	1.1837423760059878
1105	17	1.2057484013605517
1105	18	1.1721897204334581
1105	19	1.1530779542722698
1105	20	1.1850201996035779
1105	21	1.1841675840620383
1105	22	1.3144540672638456
1105	23	1.2678049399069593
1105	24	1.211223435810716
1105	25	1.224754788293403
1106	1	0.7585875084712264
1106	2	0.8004761836153413
1106	3	0.7800076470407724
1106	4	0.8324743179138494
1106	5	0.8603930608191064
1106	6	0.8096896563887199
1106	7	0.8289398381435831
1106	8	0.8502065341500327
1106	9	1.0040838999978732
1106	10	0.9952896250944931
1106	11	0.9983874083970505
1106	12	1.0123508734063904
1106	13	1.0016731286399434
1106	14	1.2174266572719006
1106	15	1.3178417676962795
1106	16	1.1668267345297139
1106	17	1.2141973971997118
1106	18	1.145926070004606
1106	19	1.1686086498996247
1106	20	1.1979695183964836
1106	21	1.188884586450392
1106	22	1.2194805044080326
1106	23	1.185368742048972
1106	24	1.2139876945943087
1106	25	1.1995640202643258
1107	1	0.6144593849157438
1107	2	0.6590392801158735
1107	3	0.6594642367571737
1107	4	0.6959059463772945
1107	5	0.7067313519612171
1107	6	0.6192354775994389
1107	7	0.6316482791075941
1107	8	0.5130392828788573
1107	9	0.69433324061837
1107	10	0.7652561349602607
1107	11	0.7115373843784099
1107	12	0.6196930981664295
1107	13	0.6574656982713307
1107	14	0.7513126457973414
1107	15	0.7320082304311413
1107	16	0.7394672435435012
1107	17	0.815630811199398
1107	18	0.8264895562414765
1107	19	0.8310611791071878
1107	20	0.801783949517386
1107	21	0.868929179538469
1107	22	0.8489837718487365
1107	23	0.78690185604232
1107	24	0.7929335484341138
1107	25	0.6870335437889921
1108	1	0.5709236083641684
1108	2	0.6858937115194337
1108	3	0.5942684138335785
1108	4	0.6600161608029467
1108	5	0.7332604602884061
1108	6	0.6104948912989556
1108	7	0.5656652102864825
1108	8	0.5677492780259712
1108	9	0.6580498320952657
1108	10	0.6557570606032712
1108	11	0.6530637067897587
1108	12	0.7082744379223485
1108	13	0.6220522144386251
1108	14	0.6908043509575705
1108	15	0.7747459768948204
1108	16	0.7075460400278502
1108	17	0.814940623015282
1108	18	0.795139914815806
1108	19	0.7595745784637344
1108	20	0.8062647806189887
1108	21	0.7294788137746764
1108	22	0.7595918204494865
1108	23	0.6644222112016536
1108	24	0.6622974609383832
1108	25	0.6821375213578733
1109	1	0.2790574820919929
1109	2	0.46631572458574766
1109	3	0.3834765675572065
1109	4	0.44907529461649887
1109	5	0.4415038621936489
1109	6	0.2318096655431603
1109	7	0.24156646447566743
1109	8	0.275312747981836
1109	9	0.3911405250049711
1109	10	0.34161842939088416
1109	11	0.40122967160774436
1109	12	0.36875764528024035
1109	13	0.3224116266584929
1109	14	0.37624163097010666
1109	15	0.374479164257167
1109	16	0.3621156413144959
1109	17	0.3720029308155901
1109	18	0.3239864824016365
1109	19	0.33828896734519276
1109	20	0.331023142400376
1109	21	0.32819686802158543
1109	22	0.40931034718231274
1109	23	0.32079752930984995
1109	24	0.33937194001579485
1109	25	0.37806899067587807
1110	1	-0.006118604419832252
1110	2	0.2162098481166801
1110	3	0.12619113175119878
1110	4	0.08387663804945333
1110	5	0.12515485827100292
1110	6	0.06170524153384349
1110	7	-0.14381661293728598
1110	8	-0.040835273361018665
1110	9	-0.06390449932411713
1110	10	-0.07208730549412934
1110	11	0.007608347098305046
1110	12	-0.15372424686246688
1110	13	-0.04845881600861901
1110	14	-0.008279279257500605
1110	15	-0.08784981495365685
1110	16	-0.14708232164147717
1110	17	-0.047868026443858014
1110	18	-0.009744610522780306
1110	19	-0.016844289298063586
1110	20	-0.05656558900449937
1110	21	-0.08930045612135018
1110	22	0.03278788971579871
1110	23	-0.05859838283141272
1110	24	-0.025216114164337
1110	25	0.1189429006290652
1111	1	-0.1782480137301512
1111	2	-0.12564951994938767
1111	3	-0.2274188695320838
1111	4	-0.2088171142312376
1111	5	-0.1345933890453921
1111	6	-0.2549549131338935
1111	7	-0.23704411015743432
1111	8	-0.2788116021851792
1111	9	-0.2829079699208279
1111	10	-0.21800514246856295
1111	11	-0.2373328334207585
1111	12	-0.16008502402950597
1111	13	-0.15147467206882936
1111	14	-0.2707690900701891
1111	15	-0.221120083460697
1111	16	-0.26638152062976417
1111	17	-0.2111413561878237
1111	18	-0.2735757019361884
1111	19	-0.3123273517680829
1111	20	-0.2731895393479391
1111	21	-0.17090278065404618
1111	22	-0.10531103196886704
1111	23	-0.33313240450095805
1111	24	-0.27725162164500716
1111	25	-0.37421125594998017
1112	1	-0.35276262126845737
1112	2	-0.2414262465623871
1112	3	-0.37813037924197346
1112	4	-0.3197779908945009
1112	5	-0.2829432486216632
1112	6	-0.41698463655325213
1112	7	-0.5174955756748574
1112	8	-0.41197365055463564
1112	9	-0.6422066610913859
1112	10	-0.6469871435968528
1112	11	-0.47671605783808246
1112	12	-0.6728664754399034
1112	13	-0.5579741096253557
1112	14	-0.8197814284399954
1112	15	-0.7013876064841327
1112	16	-0.605570799021244
1112	17	-0.6357449407186877
1112	18	-0.6253698387777007
1112	19	-0.5569900592137742
1112	20	-0.5846486191182834
1112	21	-0.6795844852830939
1112	22	-0.6746591215324642
1112	23	-0.7067413181242017
1112	24	-0.7309080486420214
1112	25	-0.8259384303741442
1113	1	-0.5440193442952577
1113	2	-0.495258119328966
1113	3	-0.5972840366804952
1113	4	-0.4646839306012609
1113	5	-0.5582677057132486
1113	6	-0.6342763496205635
1113	7	-0.6623896779908307
1113	8	-0.6265246216517184
1113	9	-0.6309357923825871
1113	10	-0.6500474951184643
1113	11	-0.5519442846625608
1113	12	-0.6140839871097086
1113	13	-0.720982507868122
1113	14	-0.7540678261328324
1113	15	-0.7927420909188001
1113	16	-0.7162878451084538
1113	17	-0.738441897209718
1113	18	-0.7277532974136776
1113	19	-0.7580157819926328
1113	20	-0.8546039831482872
1113	21	-0.8857263412289953
1113	22	-0.8279102930803148
1113	23	-0.9390369240204066
1113	24	-1.0768395824603587
1113	25	-1.1670086565956908
1114	1	-0.7247836333449555
1114	2	-0.6223790442666939
1114	3	-0.7017050772322726
1114	4	-0.6963993330107385
1114	5	-0.842893985765528
1114	6	-0.8319913316729668
1114	7	-0.7007942421937159
1114	8	-0.7655492917934623
1114	9	-0.9026391356795855
1114	10	-0.885503092762562
1114	11	-0.8694054927738719
1114	12	-1.0875546053864866
1114	13	-0.9290216308751909
1114	14	-0.9767044350495411
1114	15	-0.9738609888858392
1114	16	-0.9245048114935202
1114	17	-1.2487972637842262
1114	18	-1.154576897388445
1114	19	-1.0617538532510835
1114	20	-1.0541110938717964
1114	21	-1.0366464508768871
1114	22	-0.9536141771987161
1114	23	-1.2816084124616935
1114	24	-1.119280906033545
1114	25	-1.1897212848386758
1115	1	-1.2467662217500433
1115	2	-1.2345202840639224
1115	3	-1.3462811971657693
1115	4	-1.408537510831998
1115	5	-1.2518322408622211
1115	6	-1.3259040603070815
1115	7	-1.4146468108916679
1115	8	-1.4534933453887788
1115	9	-2.0503482568009375
1115	10	-1.8135611781310885
1115	11	-1.6905357470220466
1115	12	-1.608331090926228
1115	13	-1.6634282972703787
1115	14	-1.8218652029931164
1115	15	-1.9105300135896712
1115	16	-1.9619263562509275
1115	17	-1.8906827907076789
1115	18	-1.7468406730447228
1115	19	-1.8350986263791853
1115	20	-1.876813183496779
1115	21	-1.8547957294658772
1115	22	-2.0476842345776163
1115	23	-1.845198361478218
1115	24	-2.1521395383909265
1115	25	-1.9692896661803232
1116	1	-1.452846436805931
1116	2	-1.5859700183662326
1116	3	-1.5345741542045062
1116	4	-1.7768426716612673
1116	5	-1.4302307088677608
1116	6	-1.4725820977759554
1116	7	-1.5299311559568523
1116	8	-1.5497034347847958
1116	9	-1.965363032928984
1116	10	-2.7872053867982203
1116	11	-2.124327236210526
1116	12	-1.8746807533479952
1116	13	-1.9315518257131146
1116	14	-2.0459076343128686
1116	15	-2.835221739860536
1116	16	-2.3322172425257364
1116	17	-2.1748273554005664
1116	18	-2.2134373093396533
1116	19	-2.134673992675559
1116	20	-2.22554144058293
1116	21	-2.1103421269254596
1116	22	-2.272507362197423
1116	23	-2.4188510555742653
1116	24	-2.2253857055343502
1116	25	-2.4309523509786217
1201	1	1.10931263642577
1201	2	1.16262497423347
1201	3	1.1341565378925722
1201	4	1.1638272531942206
1201	5	1.0565208044547916
1201	6	1.0061154907818164
1201	7	1.0060424481865766
1201	8	1.0490162593218386
1201	9	1.2150325490359393
1201	10	1.2959411961528602
1201	11	1.26457180493815
1201	12	1.1902147372890681
1201	13	1.2559583268415864
1201	14	1.4773762585298371
1201	15	1.4239491304857808
1201	16	1.3957541716174262
1201	17	1.351705567287894
1201	18	1.5009436004558054
1201	19	1.3707155407555973
1201	20	1.416208183116943
1201	21	1.3921108576952363
1201	22	1.5259386328365991
1201	23	1.5457945054432187
1201	24	1.53571235061532
1201	25	1.7218645500410332
1202	1	1.0437169230817709
1202	2	1.071702598709681
1202	3	1.00392516034508
1202	4	1.0007677383299125
1202	5	1.073644955674773
1202	6	1.0126629671450544
1202	7	1.0957597059068505
1202	8	1.1075662341984085
1202	9	1.1207868511347812
1202	10	1.2439249258524399
1202	11	1.1442368681615989
1202	12	1.1792836261881945
1202	13	1.2534772731327664
1202	14	1.4416525885733051
1202	15	1.3497841963368202
1202	16	1.2996097533976396
1202	17	1.3261329721069828
1202	18	1.4171726431379668
1202	19	1.373837645222956
1202	20	1.2598063189125313
1202	21	1.4405657669051593
1202	22	1.466835769687826
1202	23	1.4622515028939986
1202	24	1.4830168834348143
1202	25	1.5504449265697602
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2211	6	-0.4042709951190986
2211	7	-0.523202558244094
2211	8	-0.3984243318393297
2211	9	-0.4170433375444631
2211	10	-0.5414052628268067
2211	11	-0.4833574714610407
2211	12	-0.5509993926797279
2211	13	-0.49383404762194516
2211	14	-0.6613662793512276
2211	15	-0.579932784795254
2211	16	-0.6067581711306644
2211	17	-0.6044802354401853
2211	18	-0.736978300258258
2211	19	-0.7355019774185507
2211	20	-0.5911370773138884
2211	21	-0.6248115915854058
2211	22	-0.6260887586140598
2211	23	-0.6679371763512876
2211	24	-0.6010397203387683
2211	25	-0.6809229979585467
2212	1	-0.6323824322409841
2212	2	-0.5733087942933466
2212	3	-0.5618204561275348
2212	4	-0.5825779837363925
2212	5	-0.6413229494020598
2212	6	-0.6166861728221562
2212	7	-0.6295008048331425
2212	8	-0.6856559710423724
2212	9	-0.8024736318173922
2212	10	-0.7797742571858777
2212	11	-0.7949241483165679
2212	12	-0.6405936906206477
2212	13	-0.7087066405027116
2212	14	-0.8588100785009161
2212	15	-0.7815389203442038
2212	16	-0.912517889918
2212	17	-0.8284523642644857
2212	18	-0.823075918842747
2212	19	-0.8102274338333686
2212	20	-0.8188422208379365
2212	21	-0.8961169240665825
2212	22	-0.738408485181985
2212	23	-0.7996673844819995
2212	24	-0.8587204105880275
2212	25	-0.9611137612897451
2213	1	-1.0792761480389927
2213	2	-0.9018684283743603
2213	3	-0.9497697503809768
2213	4	-0.9619577885355426
2213	5	-0.8386367169712443
2213	6	-0.9313891087430051
2213	7	-0.9383491507066424
2213	8	-0.9914613113059758
2213	9	-0.9153642379864948
2213	10	-1.1684144296264733
2213	11	-1.0381143535000774
2213	12	-1.0751188317076696
2213	13	-1.078577120477533
2213	14	-1.23355055908781
2213	15	-1.2227481927907036
2213	16	-1.2656852183941538
2213	17	-1.1818832068433807
2213	18	-1.2082719917513813
2213	19	-1.171214468831181
2213	20	-1.2969410979374487
2213	21	-1.2368497993060572
2213	22	-1.2568669303892008
2213	23	-1.2506618184575018
2213	24	-1.1676383588504322
2213	25	-1.145623532977659
2214	1	-1.259115259176415
2214	2	-1.343707939868672
2214	3	-1.302194173556856
2214	4	-1.3751381266059823
2214	5	-1.348229805962525
2214	6	-1.3727668311394297
2214	7	-1.4095808320474248
2214	8	-1.45564538680663
2214	9	-1.5306064005138182
2214	10	-2.0084969258530236
2214	11	-1.9532814727646652
2214	12	-1.8237211306645165
2214	13	-1.8308295334658027
2214	14	-2.17518847291214
2214	15	-2.087767029974266
2214	16	-1.9930409611703936
2214	17	-2.0985604501226334
2214	18	-1.9190872640825987
2214	19	-2.0614363938719933
2214	20	-2.124398295095915
2214	21	-2.048299141251526
2214	22	-1.9098457708112946
2214	23	-2.135116279483455
2214	24	-2.054143601403581
2214	25	-1.9021783632862181
2215	1	-1.7642258152766779
2215	2	-1.5439343124306681
2215	3	-1.5786611763476763
2215	4	-2.2843181575234865
2215	5	-1.890034289608245
2215	6	-1.580704943818965
2215	7	-1.7307554250563513
2215	8	-1.911451745265257
2215	9	-2.027452165183057
2215	10	-2.0413406968161993
2215	11	-2.125136834753363
2215	12	-2.196821185064735
2215	13	-2.1578124532755467
2215	14	-2.328158053967016
2215	15	-2.3184750307700526
2215	16	-2.3392839771884724
2215	17	-2.4120347299669973
2215	18	-2.726439933669809
2215	19	-3.128711423588676
2215	20	-2.7089998881056374
2215	21	-2.537726963992931
2215	22	-3.466534752401614
2215	23	-2.7720200270220374
2215	24	-2.772219270818269
2215	25	-2.6748109298115637
2216	1	-3.2691775563647774
2216	2	-5.88599186778406
2216	3	-3.662723972979176
2216	4	-6.229083213037274
2216	5	-3.9517751802965044
2216	6	-2.898980569901642
2216	7	-2.735110407313986
2216	8	-2.6307768607921744
2216	9	-2.8394941085686938
2216	10	-2.8186139325733635
2216	11	-2.755571206087886
2216	12	-2.9191239256313395
2216	13	-2.83857115542585
2216	14	-3.4557661491588902
2216	15	-3.358952223971855
2216	16	-3.332381151405862
2216	17	-3.2125770360964623
2216	18	-3.1340060624446906
2216	19	-3.200998617991395
2216	20	-3.503918842416361
2216	21	-3.6811148267984493
2216	22	-3.6873869289468146
2216	23	-3.5866891898487765
2216	24	-3.635991280155835
2216	25	-3.978119877328041
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	119020.0
3	9.0
4	753.0
5	5238.0
6	7609.0
7	7527.0
8	7007.0
9	5780.0
10	5339.0
11	5314.0
12	5254.0
13	4781.0
14	4566.0
15	4449.0
16	4503.0
17	4818.0
18	5649.0
19	7257.0
20	9854.0
21	12349.0
22	15388.0
23	18419.0
24	20607.0
25	23382.0
26	25723.0
27	28590.0
28	31458.0
29	37740.0
30	47037.0
31	59778.0
32	76352.0
33	99924.0
34	136151.0
35	188715.0
36	277446.0
37	485340.0
38	1348025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72949842955155	17.742605971373013	12.717808997098379	24.81008660197706
2	16.459470416816927	21.213725990252588	37.98502273660323	24.341780856327258
3	18.239700194739005	25.48303114383964	29.33556715135397	26.941701510067382
4	12.64798098059524	16.987102754203384	37.399452308740145	32.965463956461235
5	15.310885630412175	35.85090831220985	33.05515108234683	15.783054975031138
6	31.674102555730755	37.090361897006375	16.664201907915334	14.571333639347536
7	28.171740345624436	31.784987766951623	21.704008252265243	18.339263635158698
8	26.52411716989866	32.52637590376565	20.228568757894845	20.720938168440853
9	27.25679890788566	15.301891835229009	19.312339259622018	38.12896999726331
10	16.38339493362659	26.837542935639107	31.019631012749787	25.759431117984512
11	34.74736727206845	23.195822333957842	23.078090204321903	18.978720189651803
12	24.683682478221257	24.84537046106844	28.55731168359733	21.91363537711297
13	29.041959391782648	21.491644069733532	25.119456175500943	24.346940362982878
14	23.640469552946357	21.138302218044778	25.921453478172346	29.299774750836523
15	24.322984126293065	27.847045664518205	23.631386569229225	24.198583639959512
16	24.20037286056526	27.39308748266621	24.221019758280846	24.185519898487687
17	24.081732219035892	26.05877144289482	25.14333678430047	24.716159553768822
18	25.431336681113386	24.801883174299906	26.234880726531873	23.531899418054834
19	26.173080050659948	25.23338664836284	25.68232779650989	22.911205504467322
20	25.279739599039374	25.26395752862381	25.685047496669117	23.771255375667696
21	25.290347405312176	25.406710450329783	25.772861897072964	23.530080247285078
22	24.731421356248116	25.08070085123671	26.073954410542026	24.113923381973144
23	23.977307757931065	24.779385425913294	26.24195433445576	25.001352481699886
24	23.819042198261208	25.92357715561093	26.161910353424606	24.095470292703258
25	24.071223114858796	25.41350735744893	26.18534720788295	24.32992231980933
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15569.0
1	15569.0
2	13095.0
3	10621.0
4	10621.0
5	10621.0
6	10213.0
7	9805.0
8	9805.0
9	9805.0
10	11034.0
11	12263.0
12	12263.0
13	12263.0
14	14605.5
15	16948.0
16	16948.0
17	16948.0
18	27808.5
19	38669.0
20	38669.0
21	38669.0
22	54664.5
23	70660.0
24	70660.0
25	70660.0
26	94808.5
27	118957.0
28	118957.0
29	118957.0
30	141101.5
31	163246.0
32	163246.0
33	163246.0
34	189713.0
35	216180.0
36	216180.0
37	216180.0
38	241197.0
39	266214.0
40	266214.0
41	266214.0
42	298112.0
43	330010.0
44	330010.0
45	330010.0
46	352230.0
47	374450.0
48	374450.0
49	374450.0
50	381533.5
51	388617.0
52	388617.0
53	388617.0
54	364059.0
55	339501.0
56	339501.0
57	339501.0
58	309728.5
59	279956.0
60	279956.0
61	279956.0
62	241393.0
63	202830.0
64	202830.0
65	202830.0
66	166233.0
67	129636.0
68	129636.0
69	129636.0
70	100518.5
71	71401.0
72	71401.0
73	71401.0
74	57061.5
75	42722.0
76	42722.0
77	42722.0
78	34191.5
79	25661.0
80	25661.0
81	25661.0
82	19249.5
83	12838.0
84	12838.0
85	12838.0
86	10012.5
87	7187.0
88	7187.0
89	7187.0
90	5052.5
91	2918.0
92	2918.0
93	2918.0
94	1580.5
95	243.0
96	243.0
97	243.0
98	146.0
99	49.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.40341248322689316
2	0.6994897925139276
3	0.7135342409690543
4	0.7232890954390176
5	0.7327262022063765
6	0.7344102650301813
7	0.7513144428087499
8	0.739621327352898
9	0.7287861306940786
10	0.7215097083044315
11	0.7343149407193998
12	0.7382232374614374
13	0.7372382195833628
14	0.7466753263507216
15	0.7425446062168609
16	0.7370157961915396
17	0.7431801016220703
18	0.746357578648117
19	0.749217307971559
20	0.7420362098926934
21	0.740574570460712
22	0.7491855332012987
23	0.7392082553395118
24	0.7426717052979027
25	0.7449912635269169
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3147151.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.18005065255511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.02905550953439	45.21849478935959
2	8.121904425862121	8.638465775251184
3	2.3749537988639795	3.789004899631939
4	1.1709822845434363	2.490915888210586
5	0.6734255909031467	1.79064035174781
6	0.4557937792860422	1.4543481761730752
7	0.3343076333818833	1.2444947813749065
8	0.24506744300336816	1.0426159225769025
9	0.19244188351713215	0.9210662201802781
>10	1.211096305799788	11.923436890447846
>50	0.09964282646230964	3.699925815867264
>100	0.07567722196863552	8.38752365988595
>500	0.010534526742104323	3.8467083646950813
>1k	0.004815783653376845	4.081107201540032
>5k	1.805918870016317E-4	0.6701591440195087
>10k+	1.2039459133442114E-4	0.8010921190381286
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14408	0.4578108899128132	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	10616	0.3373209610851211	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9116	0.28965880569442015	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6229	0.19792504395245097	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5589	0.17758919098575188	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	4751	0.15096193350748024	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4497	0.14289114186132157	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4066	0.12919621587906013	No Hit
GTACAAAGGGCAGGGACTTAATCAA	3633	0.11543774035627778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.1774770260467325E-5	0.0
5	0.0	0.0	0.0	3.1774770260467325E-5	0.0
6	3.1774770260467325E-5	0.0	0.0	3.1774770260467325E-5	0.0
7	6.354954052093465E-5	0.0	0.0	3.1774770260467325E-5	0.0
8	6.354954052093465E-5	0.0	0.0	3.1774770260467325E-5	0.0
9	6.354954052093465E-5	0.0	0.0	6.354954052093465E-5	0.0
10	6.354954052093465E-5	0.0	0.0	6.354954052093465E-5	0.0
11	6.354954052093465E-5	0.0	0.0	9.532431078140197E-5	0.0
12	6.354954052093465E-5	0.0	0.0	9.532431078140197E-5	3.1774770260467325E-5
13	6.354954052093465E-5	0.0	0.0	9.532431078140197E-5	3.1774770260467325E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2630	0.0	14.780551	1
GTATCAA	8180	0.0	12.920357	1
TAGATCG	75	1.480036E-5	12.666399	5
GCGTTAT	140	6.7666406E-10	11.541014	1
TATACGG	60	0.0058788336	11.083099	5
CCGTCCA	160	5.456968E-10	10.686591	9
TCTATAC	205	3.6379788E-12	10.194907	3
ACCGCTT	75	0.0026507224	10.132471	8
CCTATTA	265	0.0	10.038167	2
GTCTAAT	190	9.822543E-11	10.004594	1
CCCTATT	270	0.0	9.856378	1
GGTTAGG	135	6.4083724E-7	9.856378	1
TTTTCGG	290	0.0	9.827537	16
TCGAACG	215	1.0913936E-11	9.720726	3
AACGTCT	255	0.0	9.685606	6
GTTTTCG	305	0.0	9.655844	15
TTTCGTG	90	0.001114228	9.499952	18
TAGACCG	120	1.7020091E-5	9.4998	5
ATCGCCA	365	0.0	9.369816	16
CGAACGA	265	0.0	9.320708	16
>>END_MODULE