FastQCFastQC Report
Thu 2 Feb 2017
SRR4050236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147151
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT240280.7634841798185089No Hit
TATCAACGCAGAGTACTTTTTTTTT170100.5404888421305493No Hit
GTACTTTTTTTTTTTTTTTTTTTTT109880.34914117562201497No Hit
GGTATCAACGCAGAGTACTTTTTTT99040.3146973246596684No Hit
ACGCAGAGTACTTTTTTTTTTTTTT90430.287339247465406No Hit
GAGTACTTTTTTTTTTTTTTTTTTT72450.23020821053708573No Hit
GCAGAGTACTTTTTTTTTTTTTTTT45530.14467052899590774No Hit
GTACAAAGGGCAGGGACTTAATCAA36670.11651808254513368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.016.3916071
CGTCTTA604.1184074E-412.6577815
GTATCAA86200.012.49519351
GTATTAG4200.011.81164551
TCGATTA1803.6379788E-1110.54814913
CGGTTCT4700.010.50326412
GCTCGTA2151.8189894E-1210.1558229
TAGGACG1054.050899E-59.9650334
CGTCGTA3300.09.78116610
GTATAAA4300.09.7620391
TAAACGC3350.09.63502618
ATAAGAC2303.6379788E-129.5120763
CGTAGAC800.0044705259.5120753
CGGTCCA3100.09.49348510
ACCGTCC1401.0694293E-69.49348458
ACGTCTG2600.09.4933347
TCGTTTG900.00112151919.49318414
ATTAGCT3750.09.3852483
CTCGTAG2357.2759576E-129.29149610
GTCTTAC1856.2373147E-99.2823311
Produced by FastQC (version 0.11.5)