##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050236_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3147151 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.64891007771791 31.0 31.0 34.0 27.0 34.0 2 30.63532858766548 31.0 31.0 34.0 27.0 34.0 3 30.841489969817147 31.0 31.0 34.0 27.0 34.0 4 34.42683747935831 37.0 35.0 37.0 30.0 37.0 5 34.347770729780684 37.0 35.0 37.0 30.0 37.0 6 34.37354261044354 37.0 35.0 37.0 31.0 37.0 7 34.248327455530415 37.0 35.0 37.0 30.0 37.0 8 34.21281629003502 37.0 35.0 37.0 30.0 37.0 9 35.79606475825278 39.0 37.0 39.0 30.0 39.0 10 35.6967774981245 39.0 37.0 39.0 30.0 39.0 11 35.685999813799846 39.0 37.0 39.0 30.0 39.0 12 35.60196031267645 39.0 35.0 39.0 30.0 39.0 13 35.589183677554715 39.0 37.0 39.0 30.0 39.0 14 36.73604031074454 40.0 37.0 41.0 30.0 41.0 15 36.70568555496702 40.0 37.0 41.0 30.0 41.0 16 36.681533234344336 40.0 37.0 41.0 30.0 41.0 17 36.69438390468078 40.0 37.0 41.0 30.0 41.0 18 36.674750274136834 40.0 37.0 41.0 30.0 41.0 19 36.71295562240261 40.0 37.0 41.0 30.0 41.0 20 36.68907561156106 40.0 37.0 41.0 30.0 41.0 21 36.610739363951716 40.0 37.0 41.0 30.0 41.0 22 36.547434806909486 40.0 37.0 41.0 30.0 41.0 23 36.444046059435976 40.0 37.0 41.0 30.0 41.0 24 36.37012936462216 40.0 37.0 41.0 30.0 41.0 25 36.09199590359662 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8453787679540348 1101 2 0.9282407967653228 1101 3 0.9310535302038794 1101 4 0.7309769183323027 1101 5 0.915870192344606 1101 6 0.8386119777196583 1101 7 0.9324543958106659 1101 8 0.9173767494780236 1101 9 1.0598362231889098 1101 10 1.0620234509344328 1101 11 1.1038919761194137 1101 12 1.1499454164111995 1101 13 1.1193071175831335 1101 14 1.2756322828297826 1101 15 1.3894936704920156 1101 16 1.356343130265735 1101 17 1.303853963068292 1101 18 1.2756735444536176 1101 19 1.330448100073312 1101 20 1.317829761692174 1101 21 1.2990065937600264 1101 22 1.1926001908089958 1101 23 1.2570628200501304 1101 24 1.3643666314583598 1101 25 1.3884592030905836 1102 1 0.9072184800140448 1102 2 0.9474095701494072 1102 3 0.8120586457764318 1102 4 0.7361175259237243 1102 5 0.766319356189257 1102 6 0.8446761930119138 1102 7 0.8844066597877642 1102 8 0.8370306469406046 1102 9 0.9194623717908073 1102 10 0.9174687480904851 1102 11 0.903603063409065 1102 12 1.0020367951385722 1102 13 1.093140901537197 1102 14 1.2747006089470716 1102 15 1.2867771776057566 1102 16 1.190134075026549 1102 17 1.1975623591211715 1102 18 1.1947144120927717 1102 19 1.2094272297537998 1102 20 1.2298479723823945 1102 21 1.1731996096056463 1102 22 1.2646710479746588 1102 23 1.3154637096484194 1102 24 1.3435462027712717 1102 25 1.4032549706514175 1103 1 0.8123641469495269 1103 2 0.8372887389685211 1103 3 0.7961179057140697 1103 4 0.7031576921385678 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-1.805825917383828 2216 13 -1.6991086868283034 2216 14 -2.0085718761439253 2216 15 -2.0768706031255135 2216 16 -1.9870703182730978 2216 17 -2.0367708270963405 2216 18 -2.1734506228064348 2216 19 -2.0981482048543043 2216 20 -2.186836317167973 2216 21 -2.1298152065417497 2216 22 -2.218663443844875 2216 23 -2.1487743264694927 2216 24 -2.27273660079004 2216 25 -2.3019132657535266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51391.0 3 2.0 4 899.0 5 6769.0 6 9068.0 7 7431.0 8 5887.0 9 5121.0 10 4477.0 11 4247.0 12 3894.0 13 3632.0 14 3425.0 15 3518.0 16 3525.0 17 3922.0 18 4442.0 19 5962.0 20 8262.0 21 10744.0 22 13452.0 23 15585.0 24 18419.0 25 20699.0 26 22744.0 27 25566.0 28 29514.0 29 35865.0 30 46127.0 31 60017.0 32 77809.0 33 102946.0 34 140167.0 35 192810.0 36 280340.0 37 489739.0 38 1428734.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2080057320582 18.409513429325095 13.333546104610061 25.04893473400665 2 16.596144789950092 21.838002627252372 36.78915950167365 24.776693081123884 3 17.96653544745708 25.841753382662603 29.537032064873912 26.6546791050064 4 12.927491689680714 17.566780457904073 36.421553159827916 33.0841746925873 5 14.989478951058901 36.286367324177846 32.93462308354104 15.789530641222205 6 31.46106955257928 37.51823086537961 16.54071951996399 14.47998006207713 7 27.794413904864797 32.039496679482696 21.72253820978043 18.443551205872073 8 26.535889479126084 32.70683739554782 20.15888106448597 20.59839206084012 9 27.173994207448338 15.116113175592483 19.43113520349396 38.27875741346522 10 16.278602741084107 27.129627628948267 31.056256664916894 25.53551296505073 11 34.70814007395359 23.119694932714438 23.128020033624512 19.044144959707467 12 24.55625532239409 24.863524282827257 28.840417148594888 21.739803246183765 13 29.118279405886106 21.34149092820287 25.387654170344764 24.15257549556626 14 23.510500097240776 20.870285887884563 26.522040607240054 29.097173407634607 15 24.229572383796764 27.62884999321587 24.18651620199355 23.95506142099381 16 24.062107281702247 27.176400122651234 24.631228085226724 24.130264510419792 17 23.977687488838342 25.72167068009688 25.720558486450624 24.580083344614152 18 25.053326211521004 24.378332193144313 27.127336924107897 23.441004671226786 19 25.82778700312603 24.799324844318278 26.803280199140424 22.569607953415275 20 25.049157490813556 24.6700378897169 26.72149723485142 23.559307384618123 21 25.00907995796686 24.773239369935123 26.92087583225004 23.29680483984797 22 24.495409753970947 24.56747591757258 27.043940023666174 23.893174304790303 23 23.755864516754286 24.195157136479402 27.31936176263582 24.729616584130497 24 23.65118897971064 25.333199028429 27.148698407533615 23.866913584326745 25 23.82919808183562 24.772995713015103 27.33206290374192 24.065743301407355 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2114.0 1 2114.0 2 3130.5 3 4147.0 4 4147.0 5 4147.0 6 9141.5 7 14136.0 8 14136.0 9 14136.0 10 14753.5 11 15371.0 12 15371.0 13 15371.0 14 16123.0 15 16875.0 16 16875.0 17 16875.0 18 28706.0 19 40537.0 20 40537.0 21 40537.0 22 57789.5 23 75042.0 24 75042.0 25 75042.0 26 102084.0 27 129126.0 28 129126.0 29 129126.0 30 150216.0 31 171306.0 32 171306.0 33 171306.0 34 195250.0 35 219194.0 36 219194.0 37 219194.0 38 241477.5 39 263761.0 40 263761.0 41 263761.0 42 296180.5 43 328600.0 44 328600.0 45 328600.0 46 349733.0 47 370866.0 48 370866.0 49 370866.0 50 377912.0 51 384958.0 52 384958.0 53 384958.0 54 360355.5 55 335753.0 56 335753.0 57 335753.0 58 308145.5 59 280538.0 60 280538.0 61 280538.0 62 241447.5 63 202357.0 64 202357.0 65 202357.0 66 165977.0 67 129597.0 68 129597.0 69 129597.0 70 100387.5 71 71178.0 72 71178.0 73 71178.0 74 56861.0 75 42544.0 76 42544.0 77 42544.0 78 34213.5 79 25883.0 80 25883.0 81 25883.0 82 19584.5 83 13286.0 84 13286.0 85 13286.0 86 10097.5 87 6909.0 88 6909.0 89 6909.0 90 4833.5 91 2758.0 92 2758.0 93 2758.0 94 1508.0 95 258.0 96 258.0 97 258.0 98 157.5 99 57.0 100 57.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4415422075394539 2 0.0043531435256840235 3 0.0 4 0.005083963241674772 5 0.17012211997454205 6 0.04407160635126818 7 0.0016205132832838335 8 5.401710944279445E-4 9 1.9064862156280396E-4 10 0.002383107769535049 11 0.0013345403509396276 12 0.00225600868849318 13 0.004607341687767762 14 0.010263250794130947 15 0.0031774770260467326 16 5.083963241674772E-4 17 0.006895125146521409 18 3.812972431256079E-4 19 7.943692565116832E-4 20 0.003908296742037481 21 0.003495224728651405 22 0.001366315121200095 23 0.001461639431981497 24 0.002637305931618788 25 0.005846557727925988 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3147151.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.37917098655392 #Duplication Level Percentage of deduplicated Percentage of total 1 84.80296893337608 44.419092099287255 2 8.315985479698993 8.711688507257062 3 2.3771849122368414 3.7354492495412908 4 1.1599044202680406 2.430193278291176 5 0.6710317133676006 1.7574042425940894 6 0.45515297112916997 1.43043211798877 7 0.3457637518008915 1.26775730735719 8 0.24929595928801382 1.0446332542243089 9 0.19615982946753707 0.9247220323536044 >10 1.2319344478030305 12.003341243132756 >50 0.1016145608649571 3.6792268330071445 >100 0.07721051616869799 8.473984266539226 >500 0.010811791563070902 3.986777629162143 >1k 0.004616270554888426 3.6467673144832213 >5k 1.822212061140168E-4 0.8333068363325382 >10k+ 1.822212061140168E-4 1.655223788448253 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 24028 0.7634841798185089 No Hit TATCAACGCAGAGTACTTTTTTTTT 17010 0.5404888421305493 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 10988 0.34914117562201497 No Hit GGTATCAACGCAGAGTACTTTTTTT 9904 0.3146973246596684 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9043 0.287339247465406 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 7245 0.23020821053708573 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 4553 0.14467052899590774 No Hit GTACAAAGGGCAGGGACTTAATCAA 3667 0.11651808254513368 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.1774770260467325E-5 0.0 0.0 0.0 3.1774770260467325E-5 7 3.1774770260467325E-5 0.0 0.0 0.0 3.1774770260467325E-5 8 3.1774770260467325E-5 0.0 0.0 0.0 3.1774770260467325E-5 9 3.1774770260467325E-5 0.0 0.0 3.1774770260467325E-5 3.1774770260467325E-5 10 3.1774770260467325E-5 0.0 0.0 3.1774770260467325E-5 3.1774770260467325E-5 11 3.1774770260467325E-5 0.0 0.0 6.354954052093465E-5 3.1774770260467325E-5 12 3.1774770260467325E-5 0.0 0.0 6.354954052093465E-5 6.354954052093465E-5 13 3.1774770260467325E-5 3.1774770260467325E-5 0.0 6.354954052093465E-5 6.354954052093465E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2945 0.0 16.391607 1 CGTCTTA 60 4.1184074E-4 12.65778 15 GTATCAA 8620 0.0 12.4951935 1 GTATTAG 420 0.0 11.8116455 1 TCGATTA 180 3.6379788E-11 10.548149 13 CGGTTCT 470 0.0 10.503264 12 GCTCGTA 215 1.8189894E-12 10.155822 9 TAGGACG 105 4.050899E-5 9.965033 4 CGTCGTA 330 0.0 9.781166 10 GTATAAA 430 0.0 9.762039 1 TAAACGC 335 0.0 9.635026 18 ATAAGAC 230 3.6379788E-12 9.512076 3 CGTAGAC 80 0.004470525 9.512075 3 CGGTCCA 310 0.0 9.493485 10 ACCGTCC 140 1.0694293E-6 9.4934845 8 ACGTCTG 260 0.0 9.493334 7 TCGTTTG 90 0.0011215191 9.493184 14 ATTAGCT 375 0.0 9.385248 3 CTCGTAG 235 7.2759576E-12 9.291496 10 GTCTTAC 185 6.2373147E-9 9.282331 1 >>END_MODULE