FastQCFastQC Report
Thu 2 Feb 2017
SRR4050235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931224
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT114240.29059651650478324No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN90530.2302845118975668No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN66600.16941288514722133No Hit
TATCAACGCAGAGTACTTTTTTTTT65740.16722527131498993No Hit
GGTATCAACGCAGAGTACTTTTTTT40440.1028687248551596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.015.1501791
GTATCAA68300.012.8539161
TAGATCG1001.930095E-611.4008615
CTACGTT600.005874492711.08431154
TCGCGTA1855.456968E-1210.7829419
TATCCCG1158.8190063E-710.7399425
GTTTTCG3050.010.58987915
CGCGTAA1909.094947E-1210.49931210
CGAGCCG4800.010.48930115
CGCGTGA1002.4026567E-510.44931710
CGTCGTA3650.010.41027710
GCGTTAT1752.3646862E-1010.3157251
AGGCCCG5450.010.283730510
CGCTTAC1303.848436E-710.23036318
GACGGCC5900.010.14366411
TAGGACG1803.947207E-1010.0286644
GTCCTAT7150.09.8335411
GGACGGC6150.09.731070510
AGATCGA1606.64204E-89.5002316
GATTACG1002.7579814E-49.49974312
Produced by FastQC (version 0.11.5)