##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050235_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3931224
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05963486181403	31.0	31.0	34.0	26.0	34.0
2	30.171663838031108	31.0	31.0	34.0	26.0	34.0
3	30.294566781236583	31.0	31.0	34.0	26.0	34.0
4	33.67461940606793	37.0	35.0	37.0	28.0	37.0
5	33.770887387744885	37.0	35.0	37.0	29.0	37.0
6	33.83989948168814	37.0	35.0	37.0	30.0	37.0
7	33.732276257979706	37.0	35.0	37.0	29.0	37.0
8	33.68041912646036	37.0	35.0	37.0	29.0	37.0
9	35.16167000404963	39.0	35.0	39.0	29.0	39.0
10	35.071218022682	39.0	35.0	39.0	28.0	39.0
11	35.09658900128815	39.0	35.0	39.0	29.0	39.0
12	34.99663565342499	39.0	35.0	39.0	27.0	39.0
13	34.98372796869372	39.0	35.0	39.0	27.0	39.0
14	35.85252506598454	40.0	36.0	41.0	27.0	41.0
15	35.99139021332796	40.0	36.0	41.0	27.0	41.0
16	36.01798244007465	40.0	36.0	41.0	27.0	41.0
17	36.01378807211189	40.0	36.0	41.0	27.0	41.0
18	35.843188533647535	40.0	36.0	41.0	27.0	41.0
19	35.984344570545964	40.0	37.0	41.0	27.0	41.0
20	35.95624314462874	40.0	37.0	41.0	27.0	41.0
21	35.92247579888605	40.0	37.0	41.0	27.0	41.0
22	35.85240322098156	40.0	36.0	41.0	27.0	41.0
23	35.75426355760954	40.0	36.0	41.0	27.0	41.0
24	35.7019956125624	40.0	36.0	41.0	27.0	41.0
25	35.425055656965874	40.0	36.0	41.0	26.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.2000243233760344
1101	2	1.1544953074753934
1101	3	1.1066777056588464
1101	4	1.2054165141509685
1101	5	1.1582368634259836
1101	6	1.0844815807773713
1101	7	1.1761154914302736
1101	8	1.1625972819600818
1101	9	1.3266142464095196
1101	10	1.4195420268528025
1101	11	1.3213860412447858
1101	12	1.367558552408724
1101	13	1.3340204415216164
1101	14	1.7870839660470992
1101	15	1.672959582525074
1101	16	1.672614793454656
1101	17	1.6361138422767212
1101	18	1.7896879653363555
1101	19	1.7075957774799235
1101	20	1.682202553173866
1101	21	1.6540687294727476
1101	22	1.7056981639174253
1101	23	1.6795467297042137
1101	24	1.6688101463830236
1101	25	1.701921532161549
1102	1	1.069204079472506
1102	2	1.0658748705046825
1102	3	0.9646227104477489
1102	4	1.1003088316348908
1102	5	0.991511933091175
1102	6	1.044247879877986
1102	7	0.9957531228946763
1102	8	0.9669094784132355
1102	9	1.1280597495057805
1102	10	1.1964692289493541
1102	11	1.1219155820578237
1102	12	1.1293607983919145
1102	13	1.1713198219069625
1102	14	1.646056637679436
1102	15	1.4791897202114228
1102	16	1.4605082705958665
1102	17	1.3317242799326934
1102	18	1.5080004188195488
1102	19	1.380187218258719
1102	20	1.38510835257091
1102	21	1.4589631722520906
1102	22	1.5174901838524377
1102	23	1.5624791795351882
1102	24	1.5915126306365295
1102	25	1.5081389392301148
1103	1	0.6111915712626441
1103	2	0.5897796328530731
1103	3	0.6271370534463117
1103	4	0.744875494165754
1103	5	0.706191421329315
1103	6	0.6221450244338129
1103	7	0.6345489913770876
1103	8	0.6888200681583783
1103	9	0.7633935720784635
1103	10	0.8220118421057165
1103	11	0.7453735288588987
1103	12	0.9012250402607762
1103	13	0.8992199589250305
1103	14	1.2155487744908626
1103	15	1.0596916831678485
1103	16	1.0599875257042655
1103	17	1.0388878887122956
1103	18	1.181777041676284
1103	19	1.0845688605191697
1103	20	1.0434087229445694
1103	21	1.0371628801482586
1103	22	1.0681039420928258
1103	23	0.9835818538789312
1103	24	1.0138803260467881
1103	25	1.0163690573180375
1104	1	0.7295610407252191
1104	2	0.730754993060085
1104	3	0.7497910676527582
1104	4	0.8138209940811905
1104	5	0.6851965716478574
1104	6	0.7735920526121589
1104	7	0.7108690688600845
1104	8	0.7866358196745722
1104	9	0.807999720853303
1104	10	0.8673472301842367
1104	11	0.8694920239137147
1104	12	0.9505599655731132
1104	13	0.9997254399604572
1104	14	1.3674622625421762
1104	15	1.2359834379883097
1104	16	1.1323233683700948
1104	17	1.0495616541172836
1104	18	1.1838242016137173
1104	19	1.1284815196016424
1104	20	1.0576842287092276
1104	21	1.033332988671411
1104	22	1.098685030258224
1104	23	1.0564751794133116
1104	24	1.137136806950032
1104	25	1.250034716927388
1105	1	0.5429512618705843
1105	2	0.6091598145314485
1105	3	0.5841808007347637
1105	4	0.6998703567641726
1105	5	0.6520408300498204
1105	6	0.6084706679534762
1105	7	0.6895565488128241
1105	8	0.676544082912315
1105	9	0.7072190462474381
1105	10	0.6906679785211836
1105	11	0.7021589840399187
1105	12	0.7237022192869418
1105	13	0.7576036191439641
1105	14	1.0405980154636936
1105	15	0.8857571575022973
1105	16	0.9890098112196881
1105	17	0.9498672165529811
1105	18	1.0814054785255465
1105	19	0.8761046359151763
1105	20	0.9279278471611008
1105	21	0.8796636441654542
1105	22	0.8533508605568088
1105	23	0.8489245343356586
1105	24	0.8618783941562711
1105	25	0.847828932582857
1106	1	0.5030006183950526
1106	2	0.4368855756193746
1106	3	0.4282283272790508
1106	4	0.547803450141835
1106	5	0.45004883892720926
1106	6	0.43253683463048986
1106	7	0.4074816694198873
1106	8	0.5062743505185523
1106	9	0.7218566919966065
1106	10	0.6899695987038044
1106	11	0.6421053337344702
1106	12	0.6278604534109604
1106	13	0.6674384573946668
1106	14	1.0253607041659123
1106	15	0.8260547555587792
1106	16	0.7633670157389361
1106	17	0.7721696395018753
1106	18	0.9348999026483966
1106	19	0.8536695692889964
1106	20	0.8894843879008434
1106	21	0.9366582499811074
1106	22	0.8790778361016507
1106	23	0.8281519050425317
1106	24	0.8804424098563857
1106	25	0.8269051670784364
1107	1	0.33997439069018043
1107	2	0.3317340259627741
1107	3	0.30084565030914945
1107	4	0.3765947674633736
1107	5	0.324544056376169
1107	6	0.28612043289678724
1107	7	0.24675051590844532
1107	8	0.2708669928512961
1107	9	0.3659084407883455
1107	10	0.33525687521829894
1107	11	0.2887106840680076
1107	12	0.2840552013131372
1107	13	0.2440149487933354
1107	14	0.5265936852537862
1107	15	0.42011442233599894
1107	16	0.22927205318764265
1107	17	0.26518518597605834
1107	18	0.3437477025753921
1107	19	0.3591724960164555
1107	20	0.3598619601850288
1107	21	0.2804123059362382
1107	22	0.33238578309534716
1107	23	0.2992148148275504
1107	24	0.34185360269723475
1107	25	0.3292793040399715
1108	1	0.3016551914177086
1108	2	0.3667467820472261
1108	3	0.2948926241805303
1108	4	0.4082872725880691
1108	5	0.2203672474390359
1108	6	0.25752137277752496
1108	7	0.2838028171150384
1108	8	0.2858367459890587
1108	9	0.3596185946539734
1108	10	0.4205417116242103
1108	11	0.36376407365190744
1108	12	0.3359807132859842
1108	13	0.3366343727151744
1108	14	0.712739896952975
1108	15	0.517793624386826
1108	16	0.5773940421901571
1108	17	0.4499466994310737
1108	18	0.6676985569907856
1108	19	0.5440308319194926
1108	20	0.4890880835928968
1108	21	0.5660097637378101
1108	22	0.5254095534684637
1108	23	0.5950454839331272
1108	24	0.550946510515935
1108	25	0.5483234421367342
1109	1	0.10487403195655176
1109	2	0.22608734153488896
1109	3	0.14727599229968646
1109	4	0.20504801273255424
1109	5	0.1684029681546022
1109	6	0.24564765593645888
1109	7	0.16463814114322872
1109	8	0.20372389234162114
1109	9	0.14730701247121658
1109	10	0.23658653307462885
1109	11	0.13047117228919092
1109	12	0.24317920108234858
1109	13	0.245634322542152
1109	14	0.42084180620216927
1109	15	0.24776313518530202
1109	16	0.34374535133578377
1109	17	0.3026754615575342
1109	18	0.3594096152034254
1109	19	0.24097798248894975
1109	20	0.24343707890838573
1109	21	0.33009361845450513
1109	22	0.26135988804551147
1109	23	0.29892892717715824
1109	24	0.2844829651499978
1109	25	0.3032206241983175
1110	1	-0.10100659795041267
1110	2	-0.09230157848949716
1110	3	-0.16728230364605778
1110	4	-0.04710226927639383
1110	5	-0.11623367727350598
1110	6	-0.08715834561868974
1110	7	-0.12643500443473243
1110	8	-0.09638218217256878
1110	9	-0.12572634274249594
1110	10	-0.1517961457434751
1110	11	-0.08157863459631187
1110	12	-0.040821702058089215
1110	13	-0.0654843369551088
1110	14	0.09013072756616936
1110	15	-0.06552335127849318
1110	16	-0.10925365674928855
1110	17	-0.17042784355258078
1110	18	-0.0256993294858745
1110	19	-0.0277686047418797
1110	20	-0.2039238745535883
1110	21	-0.050411599345892455
1110	22	-0.06844984963588274
1110	23	-0.13698079236944238
1110	24	-0.11551789960147119
1110	25	-0.18588388216655005
1111	1	-0.4049231528719339
1111	2	-0.35269170606925826
1111	3	-0.4568137573946558
1111	4	-0.3229749806871851
1111	5	-0.3632026094752092
1111	6	-0.4322059510825227
1111	7	-0.36462250496589377
1111	8	-0.36392726297825817
1111	9	-0.5483811357870394
1111	10	-0.4513778266096935
1111	11	-0.4194977066010779
1111	12	-0.3866173287183301
1111	13	-0.5120596423435941
1111	14	-0.29770392127448275
1111	15	-0.47121868628667585
1111	16	-0.47012956624514146
1111	17	-0.46571229391242497
1111	18	-0.44727663945107565
1111	19	-0.5914266173029077
1111	20	-0.5288789239992013
1111	21	-0.5385139746492129
1111	22	-0.5933702023527019
1111	23	-0.5463624700578933
1111	24	-0.6160779339952
1111	25	-0.6577107573141205
1112	1	-0.5626568076553866
1112	2	-0.5853481623934726
1112	3	-0.6375176798085569
1112	4	-0.44073209684261627
1112	5	-0.4926163109906341
1112	6	-0.6450563426191138
1112	7	-0.5757627529036
1112	8	-0.5358492451727273
1112	9	-0.6594325424580916
1112	10	-0.6364495792467793
1112	11	-0.5880744737487262
1112	12	-0.48894570472841536
1112	13	-0.6537718844299079
1112	14	-0.4253563418081825
1112	15	-0.549549586840719
1112	16	-0.7407885169078128
1112	17	-0.7753727726024877
1112	18	-0.6090705935992986
1112	19	-0.6760739119984578
1112	20	-0.6293217525376704
1112	21	-0.5944359706216389
1112	22	-0.6587645815329495
1112	23	-0.7478031752909757
1112	24	-0.6952803339017208
1112	25	-0.6381241240077529
1113	1	-0.8850292752677333
1113	2	-1.0957150268694669
1113	3	-1.0139040901796221
1113	4	-0.889205945276025
1113	5	-1.0221802655180952
1113	6	-1.0150354050447206
1113	7	-1.0275557497924694
1113	8	-1.0097580819149528
1113	9	-1.0220642748375113
1113	10	-1.1295600584201466
1113	11	-1.1125626092356384
1113	12	-1.0903186434277572
1113	13	-1.1701812320502967
1113	14	-1.0838667880020907
1113	15	-1.2141877046913763
1113	16	-1.2454083814020223
1113	17	-1.2867644283265776
1113	18	-1.261923148428501
1113	19	-1.2016756522889054
1113	20	-1.3595302818762462
1113	21	-1.2031852818634547
1113	22	-1.2548783414546065
1113	23	-1.7179623686362788
1113	24	-1.5966943789035142
1113	25	-1.6467225154328204
1114	1	-2.234070048659838
1114	2	-2.432043114796155
1114	3	-2.12572543438867
1114	4	-5.244084858899495
1114	5	-2.7951561616047336
1114	6	-2.3123860968162226
1114	7	-2.0808464098226267
1114	8	-2.246283169177289
1114	9	-2.81650907811499
1114	10	-2.624420544136541
1114	11	-2.3082708050407206
1114	12	-2.565307175416919
1114	13	-2.5317709092587464
1114	14	-2.446243408023392
1114	15	-3.161068405742739
1114	16	-3.0449973125306826
1114	17	-2.7934877144521337
1114	18	-2.787699150495378
1114	19	-2.7110731599078832
1114	20	-2.9062568238623427
1114	21	-2.848590761727209
1114	22	-2.9848604012675324
1114	23	-3.1001572272765614
1114	24	-3.103114817271525
1114	25	-3.1296687924156004
1115	1	-2.854811883963386
1115	2	-2.2636212053591684
1115	3	-1.8112133960529988
1115	4	-1.8051651367329278
1115	5	-1.684565849042123
1115	6	-1.7681208360134448
1115	7	-1.8449851929656162
1115	8	-1.782933139123191
1115	9	-2.062382734028972
1115	10	-1.9573518204338782
1115	11	-1.9710160798687468
1115	12	-2.353318262039508
1115	13	-2.5248818422714976
1115	14	-2.8497230046306186
1115	15	-2.5735984598401203
1115	16	-2.4487893882670093
1115	17	-2.4770275862213182
1115	18	-2.409444124782425
1115	19	-2.3361912892072922
1115	20	-2.532981845568081
1115	21	-2.383004122443161
1115	22	-2.534720065329516
1115	23	-2.58978731142804
1115	24	-2.8819289326792585
1115	25	-2.8874147351124577
1116	1	-1.300879282789925
1116	2	-1.2941357231867592
1116	3	-1.393836133127369
1116	4	-1.250226567955032
1116	5	-1.3357272752494325
1116	6	-1.4581549366102067
1116	7	-1.5073085468596048
1116	8	-1.511974812058348
1116	9	-1.8378729301074017
1116	10	-2.2787915676882733
1116	11	-1.9836252770002787
1116	12	-1.9227475785231292
1116	13	-1.9147033156178708
1116	14	-2.759682886281489
1116	15	-2.575281926065479
1116	16	-2.525118559321619
1116	17	-2.231753026269459
1116	18	-2.0814319889818123
1116	19	-2.028712225970743
1116	20	-2.008612455868466
1116	21	-2.2247277070176565
1116	22	-2.155905061066939
1116	23	-2.5142284126767223
1116	24	-2.5204891854248572
1116	25	-2.5351340069644195
1201	1	1.160293665068977
1201	2	1.147870081249323
1201	3	1.0964786603862358
1201	4	1.1770621069407454
1201	5	1.060392801416988
1201	6	1.046129226637376
1201	7	1.075356613011131
1201	8	1.064392191799847
1201	9	1.2703852214143296
1201	10	1.346428431464659
1201	11	1.2579129994035512
1201	12	1.3653105438217992
1201	13	1.3129957169088868
1201	14	1.7623531168190567
1201	15	1.6301652353474552
1201	16	1.50822837640807
1201	17	1.533905418230411
1201	18	1.6686679314878603
1201	19	1.590319440273511
1201	20	1.549403306328479
1201	21	1.4929869290487687
1201	22	1.5031337850913005
1201	23	1.5608399007090483
1201	24	1.5802671122941732
1201	25	1.5520334026799247
1202	1	0.9712677856989913
1202	2	0.9551081171304254
1202	3	0.9404566906913381
1202	4	1.0423912247511353
1202	5	0.9562284186285126
1202	6	0.9058470124645481
1202	7	0.9130920533274391
1202	8	0.9305480532415231
1202	9	1.1171786863781463
1202	10	1.0972487842916934
1202	11	1.120951009212412
1202	12	1.107042256025487
1202	13	1.1394830423253097
1202	14	1.4918524366989985
1202	15	1.3457582026107815
1202	16	1.379702734386747
1202	17	1.2892550266621825
1202	18	1.4764044313864133
1202	19	1.2980932445035052
1202	20	1.3799472485440774
1202	21	1.3017757501903517
1202	22	1.367044387639531
1202	23	1.4719097729906494
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2211	7	-0.28477996089146984
2211	8	-0.24999453831956941
2211	9	-0.33985748150372075
2211	10	-0.30811716367812636
2211	11	-0.31308518100338745
2211	12	-0.26292785570490906
2211	13	-0.33692472404183604
2211	14	-0.09366698695876607
2211	15	-0.2453893735194157
2211	16	-0.38840566787030895
2211	17	-0.443406888655133
2211	18	-0.287724986612929
2211	19	-0.39583116623847303
2211	20	-0.39009529020261624
2211	21	-0.38840419716834873
2211	22	-0.30707115572732846
2211	23	-0.271991573385705
2211	24	-0.24249782733640757
2211	25	-0.23141220494380121
2212	1	-0.3931689637605267
2212	2	-0.42897811116593587
2212	3	-0.4495959299972334
2212	4	-0.3866041659955286
2212	5	-0.4489638745735931
2212	6	-0.47070639739015974
2212	7	-0.5562794235737485
2212	8	-0.4799435978778064
2212	9	-0.5614060036302746
2212	10	-0.7743473933455078
2212	11	-0.6966105791953368
2212	12	-0.6373070879911324
2212	13	-0.5768505111162696
2212	14	-0.7185287862662548
2212	15	-0.7160595214391563
2212	16	-0.764628827373599
2212	17	-0.9306252560391428
2212	18	-0.5912985399492356
2212	19	-0.7764088416138364
2212	20	-0.807544002017643
2212	21	-0.7803721636337713
2212	22	-0.9724314475219202
2212	23	-0.9765911082846088
2212	24	-0.8788536206243052
2212	25	-0.8988896579445012
2213	1	-0.8899139625265668
2213	2	-0.67522486883475
2213	3	-0.697511546669638
2213	4	-0.6221158758272196
2213	5	-0.6027151688372641
2213	6	-0.7618251727844765
2213	7	-0.8351898815850731
2213	8	-0.758573194993744
2213	9	-0.84948507621273
2213	10	-0.9071518089536212
2213	11	-0.9154683337183513
2213	12	-0.8820924688923668
2213	13	-1.0100141734214603
2213	14	-0.8070865225196115
2213	15	-0.9282983469509816
2213	16	-0.9924881975098856
2213	17	-1.0326024597791132
2213	18	-0.9822332729690828
2213	19	-1.0442042916542817
2213	20	-1.0704157331424113
2213	21	-0.8867788076398071
2213	22	-1.0728890716287722
2213	23	-1.0529618558853073
2213	24	-1.0674846724857545
2213	25	-1.1006896679848808
2214	1	-1.1867922276985752
2214	2	-1.0769053980304761
2214	3	-1.0948999434849647
2214	4	-0.9064462861754166
2214	5	-0.9157751583289553
2214	6	-1.1064847263574293
2214	7	-1.0393439946319134
2214	8	-1.0957825491857989
2214	9	-1.179158408154045
2214	10	-1.2489370433129707
2214	11	-1.199959680357658
2214	12	-1.1819303501434746
2214	13	-1.313270178224009
2214	14	-1.2196087588275262
2214	15	-1.3940105429551863
2214	16	-1.272489655173139
2214	17	-1.315276482427258
2214	18	-1.2397504432238975
2214	19	-1.3437831473229807
2214	20	-1.4135480856838214
2214	21	-1.4578046911777278
2214	22	-1.4789768439969748
2214	23	-1.3997825719733257
2214	24	-1.506511237052031
2214	25	-1.4580977154811379
2215	1	-1.6332071638270804
2215	2	-1.7843916981969308
2215	3	-1.6153243739457679
2215	4	-1.864128768124779
2215	5	-1.7056392383271586
2215	6	-1.4233201376688598
2215	7	-1.6293492155987153
2215	8	-1.6819335808471791
2215	9	-2.001574799824809
2215	10	-1.6857881175248082
2215	11	-1.9142309891049791
2215	12	-1.9285705705731928
2215	13	-1.84801029981341
2215	14	-2.532067717615199
2215	15	-2.307046431064954
2215	16	-1.9902899877495628
2215	17	-2.06125046752377
2215	18	-1.9454646123615689
2215	19	-2.1292583260807234
2215	20	-2.380230115482469
2215	21	-2.1309578886660034
2215	22	-2.4900910083050505
2215	23	-2.2122899933236653
2215	24	-2.312476670421418
2215	25	-2.3601811893309232
2216	1	-1.6951153838200241
2216	2	-1.5993210241743299
2216	3	-1.5392659742258203
2216	4	-1.3529567965365317
2216	5	-1.9277198683563022
2216	6	-1.650829356174583
2216	7	-1.7356704417541593
2216	8	-2.1207237821768565
2216	9	-2.078468511120782
2216	10	-2.134856777089823
2216	11	-2.050721222386585
2216	12	-2.2988090980723115
2216	13	-2.250452188149495
2216	14	-2.1939547788795934
2216	15	-2.221393739273502
2216	16	-2.2957490822659494
2216	17	-2.394550905381159
2216	18	-2.654580603126668
2216	19	-2.4841267784571954
2216	20	-2.6181409364309545
2216	21	-2.7787929678829357
2216	22	-2.9618308280343086
2216	23	-2.5660905306616897
2216	24	-2.649747394011243
2216	25	-2.6940245654575605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	138632.0
3	8.0
4	885.0
5	5965.0
6	7970.0
7	7599.0
8	7016.0
9	6256.0
10	5884.0
11	5606.0
12	5409.0
13	5315.0
14	5014.0
15	5184.0
16	5261.0
17	5584.0
18	6169.0
19	7884.0
20	10237.0
21	13341.0
22	16760.0
23	19749.0
24	22979.0
25	26441.0
26	29517.0
27	33198.0
28	37803.0
29	45137.0
30	57147.0
31	72920.0
32	93679.0
33	124222.0
34	168726.0
35	236257.0
36	347842.0
37	606088.0
38	1737540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.898740051275055	18.038518760270627	12.677840430292314	25.384900758162004
2	16.161103763330033	20.883645717168328	38.59310122311747	24.362149296384167
3	18.282517905108687	24.91991672876161	29.473690948989965	27.32387441713974
4	12.480688264738953	16.960959629030302	38.38915608133042	32.16919602490032
5	14.797017878081128	35.9770962217884	34.11706068179618	15.108825218334292
6	31.391719395245193	37.46255248415837	17.22569078258936	13.920037338007074
7	27.97961583090207	31.498587349929945	22.15816557846932	18.363631240698666
8	25.91928060454381	33.53278689154434	20.674832813248	19.87309969066385
9	26.84043741402533	15.290538361830844	19.549939006165463	38.31908521797837
10	15.47691675669252	27.20471136149311	32.533934496011454	24.78443738580291
11	34.714949066350904	22.907937278779734	23.563744349990536	18.813369304878826
12	23.958045182111572	24.6383381998873	29.651631576251212	21.75198504174991
13	28.723523053832402	21.391144213874817	25.29549168361362	24.589841048679162
14	22.76725803510512	20.88453768565206	26.88553752789007	29.462666751352756
15	24.14977538047014	27.840630266521188	23.60469523970515	24.40489911330352
16	24.102789772456575	27.372935667253923	24.628423947873564	23.895850612415938
17	23.69949673471813	26.37803706048814	25.781767084073582	24.140699120720143
18	24.423596063907713	25.665867564120298	26.798621035862286	23.111915336109703
19	25.55411699469696	25.747567146001977	26.000437971347452	22.69787788795361
20	24.815795742413723	25.66608351754186	26.075346623669027	23.442774116375393
21	24.85455727372431	25.682954578634863	25.97967976731213	23.482808380328695
22	24.170027367997758	25.618597431736887	26.325708628795436	23.88566657146992
23	23.620294179799313	25.32479524599297	26.360456702291835	24.694453871915883
24	23.632052792376438	26.07069295117161	26.36516793347775	23.932086322974197
25	23.891233235700593	25.714112284821233	26.26812429375058	24.12653018572759
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15247.0
1	15247.0
2	14018.0
3	12789.0
4	12789.0
5	12789.0
6	9722.0
7	6655.0
8	6655.0
9	6655.0
10	8922.5
11	11190.0
12	11190.0
13	11190.0
14	16279.5
15	21369.0
16	21369.0
17	21369.0
18	35594.0
19	49819.0
20	49819.0
21	49819.0
22	71945.5
23	94072.0
24	94072.0
25	94072.0
26	127417.5
27	160763.0
28	160763.0
29	160763.0
30	193045.0
31	225327.0
32	225327.0
33	225327.0
34	262523.0
35	299719.0
36	299719.0
37	299719.0
38	330890.0
39	362061.0
40	362061.0
41	362061.0
42	395056.0
43	428051.0
44	428051.0
45	428051.0
46	449294.5
47	470538.0
48	470538.0
49	470538.0
50	472728.5
51	474919.0
52	474919.0
53	474919.0
54	439815.5
55	404712.0
56	404712.0
57	404712.0
58	364246.0
59	323780.0
60	323780.0
61	323780.0
62	278107.0
63	232434.0
64	232434.0
65	232434.0
66	190459.0
67	148484.0
68	148484.0
69	148484.0
70	115701.5
71	82919.0
72	82919.0
73	82919.0
74	66069.0
75	49219.0
76	49219.0
77	49219.0
78	39842.0
79	30465.0
80	30465.0
81	30465.0
82	23015.0
83	15565.0
84	15565.0
85	15565.0
86	11550.0
87	7535.0
88	7535.0
89	7535.0
90	5383.5
91	3232.0
92	3232.0
93	3232.0
94	1765.5
95	299.0
96	299.0
97	299.0
98	180.0
99	61.0
100	61.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23501586274402067
2	0.6623636811334078
3	0.6591840098656296
4	0.6668406582784394
5	0.6840108831244416
6	0.6976707508908166
7	0.6922525910505226
8	0.6882589239381933
9	0.6799663412718278
10	0.6814671461102191
11	0.6874958028339265
12	0.6874194907234998
13	0.6838582589035883
14	0.6768375447443341
15	0.6828407640978993
16	0.6913368457254025
17	0.6898614782571535
18	0.6828407640978993
19	0.6832223246500326
20	0.6902176014391447
21	0.6852318768912685
22	0.687546677574211
23	0.690599161991278
24	0.6951270138765941
25	0.6816197703310725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3931224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.761920862629374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.93796348418425	54.31217541530978
2	7.2082835008498325	8.903948702697686
3	1.7260663312507103	3.198155164630662
4	0.8185462071998368	2.02219944285927
5	0.4564497773769202	1.4095607514059072
6	0.3116479056759524	1.1548783972417414
7	0.23445834287352146	1.013641833269636
8	0.16983429658031848	0.8391433908123166
9	0.1350655126056423	0.7507714950738136
>10	0.8618009915188959	10.15715503275801
>50	0.07844691062629928	3.3594160084990063
>100	0.0519535248091713	6.472175903977681
>500	0.0064469221408354	2.6957170330028837
>1k	0.002869920126559817	2.845070885451518
>5k	1.247791359373832E-4	0.572523248555854
>10k+	4.1593045312461065E-5	0.29346729445425923
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11424	0.29059651650478324	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	9053	0.2302845118975668	No Hit
GNNNNNNNNNNNNNNNNNNNNNNNN	6660	0.16941288514722133	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6574	0.16722527131498993	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4044	0.1028687248551596	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5437370142225423E-5
2	0.0	2.5437370142225423E-5	0.0	2.5437370142225423E-5	2.5437370142225423E-5
3	0.0	2.5437370142225423E-5	0.0	2.5437370142225423E-5	2.5437370142225423E-5
4	0.0	2.5437370142225423E-5	0.0	2.5437370142225423E-5	2.5437370142225423E-5
5	0.0	2.5437370142225423E-5	0.0	2.5437370142225423E-5	2.5437370142225423E-5
6	0.0	5.087474028445085E-5	0.0	2.5437370142225423E-5	2.5437370142225423E-5
7	0.0	5.087474028445085E-5	0.0	2.5437370142225423E-5	2.5437370142225423E-5
8	0.0	5.087474028445085E-5	0.0	2.5437370142225423E-5	2.5437370142225423E-5
9	0.0	5.087474028445085E-5	0.0	7.631211042667628E-5	2.5437370142225423E-5
10	0.0	5.087474028445085E-5	0.0	1.017494805689017E-4	7.631211042667628E-5
11	0.0	5.087474028445085E-5	0.0	1.017494805689017E-4	7.631211042667628E-5
12	0.0	7.631211042667628E-5	0.0	1.017494805689017E-4	1.7806159099557797E-4
13	0.0	7.631211042667628E-5	0.0	1.017494805689017E-4	2.034989611378034E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2195	0.0	15.150179	1
GTATCAA	6830	0.0	12.853916	1
TAGATCG	100	1.930095E-6	11.400861	5
CTACGTT	60	0.0058744927	11.0843115	4
TCGCGTA	185	5.456968E-12	10.782941	9
TATCCCG	115	8.8190063E-7	10.739942	5
GTTTTCG	305	0.0	10.589879	15
CGCGTAA	190	9.094947E-12	10.499312	10
CGAGCCG	480	0.0	10.489301	15
CGCGTGA	100	2.4026567E-5	10.449317	10
CGTCGTA	365	0.0	10.410277	10
GCGTTAT	175	2.3646862E-10	10.315725	1
AGGCCCG	545	0.0	10.2837305	10
CGCTTAC	130	3.848436E-7	10.230363	18
GACGGCC	590	0.0	10.143664	11
TAGGACG	180	3.947207E-10	10.028664	4
GTCCTAT	715	0.0	9.833541	1
GGACGGC	615	0.0	9.7310705	10
AGATCGA	160	6.64204E-8	9.500231	6
GATTACG	100	2.7579814E-4	9.499743	12
>>END_MODULE