FastQCFastQC Report
Thu 2 Feb 2017
SRR4050235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931224
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT184250.46868354487050345No Hit
TATCAACGCAGAGTACTTTTTTTTT123040.3129814022299416No Hit
GGTATCAACGCAGAGTACTTTTTTT67260.1710917515766082No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57280.14570525617466723No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53730.1366749897741772No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41530.10564139820066218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.015.9794141
GTATCAA72350.013.4756351
TAGATCG1103.8100552E-812.0931925
TTAACCG658.0155017E-411.6945165
CGTCTTA752.0793203E-411.39765615
AACCGCG2700.010.5531177
TCGTTAA2900.010.4808712
GCGTTAT1752.237357E-1010.3473151
CGTCGTA3400.010.33650410
AGAACCG4250.010.2842955
ATACCGT4400.010.1449966
CCGTCCG951.6480571E-49.9983259
CGCGTAT951.6491153E-49.997697
ACCGTCC1554.083995E-89.8041868
CGACCAT3750.09.62505410
GAACCGC4200.09.4974426
GCGGTAA1304.267098E-69.49575118
CGCCAGT4200.09.49575118
CGGTTCT5250.09.40782912
ATAACGC4550.09.3961833
Produced by FastQC (version 0.11.5)